Labshake search
Citations for Jena Bioscience :
1 - 50 of 94 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified with 4% Glutathione Agarose (Jena Biosciences) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Genome-labeled AdV was produced by growing the virus in A549 cells in the presence of 2.5 μM EdC (5-ethynyl-2’-deoxycytidine, Jena Biosciences) as described 52 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 500 μg of protein was incubated overnight at 4°C with m7GTP agarose beads (Jena biosciences, #AC-155S). Beads were washed with cap binding buffer and suspended in 20 μL Laemmli buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were starved of methionine for one hour then 4-Azido-L-homoalanine HCl (L-AHA) (Jena Bioscience) was added to the media (final concentration 40μM ...
-
bioRxiv - Molecular Biology 2019Quote: ... 4-thio-UTP (4SU, Jena Bioscience) or 6-thio-GTP (6SG ...
-
bioRxiv - Immunology 2020Quote: ... purified naïve C57BL/6 B2 cells were preincubated with a mixture of NIP-OVA and HIV-1 p17/p24/gp120 fusion protein (Jena Biosciences) covalently bound to 1 μm beads (1:1 bead:Bcell ratio ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell lysates (500 mg of protein) were incubated with 50 μl of 7-methyl-GTP-Agarose slurry (Jena Biosciences, AC-155S) for 16 h at 4 °C ...
-
bioRxiv - Biochemistry 2020Quote: ... JBScreen Classic 1-4 and 6 (Jena Bioscience) and Crystal Screen ...
-
bioRxiv - Microbiology 2023Quote: ... JBScreen Classic 1-4 and 6 (Jena Bioscience) and Crystal Screen ...
-
bioRxiv - Immunology 2020Quote: ... and 4 mM of each ribonucleotide triphosphate (Jena Bioscience) and incubated at 37 °C overnight ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were purified by Ni2+-NTA chromatography (Jena Bioscience) and eluted stepwise in binding buffer with 40–250 mM imidazole ...
-
bioRxiv - Molecular Biology 2021Quote: ... Extracted proteins were incubated overnight with Alkyne agarose beads (Jena Bioscience), and downstream preparations were done using Click Chemistry Capture Kit following the manufacturer’s guidelines ...
-
bioRxiv - Biophysics 2023Quote: ... 50 mM each of Azide-(PEG)4-NHS (Jena Biosciences GmbH, Jena, Germany) and Methyl-(PEG)4-NHS (Life technologies ...
-
bioRxiv - Biophysics 2024Quote: ... 50 mM each of azide-(PEG)4-NHS (Jena Biosciences GmbH, Jena, Germany) and methyl-(PEG)4-NHS (Life technologies ...
-
bioRxiv - Biophysics 2022Quote: ... The 4 bp overhangs formed were filled in with biotin-11-dGTP (Jena Bioscience), biotin-16-dUTP (Roche) ...
-
bioRxiv - Neuroscience 2022Quote: ... 0.4 μM 5/6- TAMRA-PEG 4 -Alkyne (Jena Bioscience; Jena, Germany; CLK-TA108) and 0.2 mM freshly prepared CuSO4 in 1x PBS pH7.8 ...
-
bioRxiv - Biophysics 2019Quote: ... Control reactions for azide-bearing proteins were performed using TAMRA-DBCO (Jena Bioscience). Reaction mixtures were incubated for varying durations (0.5 hours to overnight ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cap-bound proteins were pulled down using γ-Aminophenyl-m7GTP beads (Jena Bioscience).
-
bioRxiv - Microbiology 2022Quote: ... Then GST-tagged proteins were pulled down using Glutathione magnetic agarose (Jena Bioscience). Beads were washed with protein binding buffer and proteins are then eluted using Laemmli buffer ...
-
bioRxiv - Neuroscience 2023Quote: ... and 32μg of protein was clicked to 31μM biotin-alkyne (Jena biosciences, #CLK-TA105) mixed in PBS pH 7.8-PI (Calbiochem ...
-
bioRxiv - Biochemistry 2023Quote: ... His-tagged and Strep-tagged proteins were purified with Ni-NTA Agarose (Jena Bioscience) (except for AqTuA that was purified with a HiScreen Ni FF column (Cytiva) ...
-
bioRxiv - Biochemistry 2020Quote: ... ∼900 μL of 4 μM forked DNA duplex and 100 μM ATPγS (Jena Bioscience, #NU-406-50) in Buffer S was applied to the column at 0.05 mL/min ...
-
bioRxiv - Molecular Biology 2021Quote: ... 4 samples each) were extracted with CHAPS Lysis buffer from the Click Chemistry Capture Kit (Jena Bioscience). Input cell numbers were adjusted to give similar amount of total injected peptides ...
-
bioRxiv - Biophysics 2021Quote: ... The protein was incubated with ~20× molar excess of Dibenzylcyclooctyne(DBCO)-Sulfo-Cy5 (Jena Bioscience) at 4°C overnight in the dark ...
-
bioRxiv - Cancer Biology 2020Quote: ... Proteins were then extracted and probes were clicked onto azide-functionalized magnetic beads (Jena Bioscience) as described above ...
-
bioRxiv - Cell Biology 2021Quote: ... Total proteins (500μg) were incubated with 15μL Immobilized γ-Aminophenyl-m7GTP (Jena Bioscience, #AC-155) at 4°C for 3 hours ...
-
bioRxiv - Biochemistry 2022Quote: ... 4 mM DMSO was used to dissolve 25 mM dibenzylcyclooctyne NHS (DBCO-NHS) ester purchased from Jena Bioscience. The DBCO-NHS ester was 33.3-fold molar excess over antibodies ...
-
bioRxiv - Immunology 2023Quote: ... they were injected intraperitoneally with 1.5mg L-AHA (Thermo) and then 4 hours later 1mg OPP (Jena Bioscience) dissolved in 100μl PBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... Quantitative protein occupancy was performed by qPCR according to the manufacturer’s instructions (Jena Bioscience, PCR-369). Mean values for the analyzed regions were expressed as fold enrichment compared to a negative control without protein occupation (SOX2 gene) ...
-
bioRxiv - Developmental Biology 2020Quote: ... 250 µg of protein was pre-cleared using 50 µL of agarose beads (Jena Biosciences, AC-100S) in Cap Lysis Buffer for 30 min at 4°C with rocking ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Alkyne labelled proteins were enriched and affinity purified by direct attachment to azide agarose beads (Jena Bioscience) using copper catalysed click chemistry ...
-
bioRxiv - Biophysics 2023Quote: ... we ran PCR reactions with added biotin-16-dUTP or 5-DBCO-(PEG)4-dUTP (Jena Biosciences GmbH, Jena, Germany), respectively ...
-
bioRxiv - Cell Biology 2024Quote: ... overnight at 4°C in click reagents (1:1 DMSO/H2O with 20 μM picolyl-azide-sulfo-Cy5 (Jena Bioscience), 300 μM CuSO4 ...
-
bioRxiv - Molecular Biology 2022Quote: C15-az and C17-az modified proteins were pulled down using the Click Chemistry Capture Kit (Jena Bioscience) and alkyne agarose beads (Jena Bioscience) ...
-
bioRxiv - Microbiology 2023Quote: ... Eluted protein was incubated with an ATP-agarose resin (Immobilized γ-Aminophenyl-ATP C10-spacer from Jena Bioscience) overnight at 4 °C with gentle agitation ...
-
bioRxiv - Microbiology 2024Quote: ... The following primary antibodies were diluted 1:400 in blocking buffer and incubated over night at 4°C: mouse anti-dsRNA J2 (RNT-SCI-10010200; Jena bioscience), mouse anti-DDX5 (67025 Proteintech ...
-
bioRxiv - Microbiology 2021Quote: ... crescentus ParBΔCTD-CTPɣS complex crystal were also set up in sitting-drop vapour diffusion format in MRC2 96-well crystallization plates with drops comprised of 0.3 µL precipitant solution and 0.3 µL of protein solution (∼10 mg/mL) supplemented with 1 mM CTPɣS (Jena Biosciences) and 1 mM MgCl2 ...
-
bioRxiv - Biophysics 2021Quote: ... GMPCPP seeds were prepared by polymerizing a 1:4 molar ratio of TAMRA labelled:unlabelled tubulin in the presence of guanosine-5’-[(α, β)-methyleno]triphosphate (GMPCPP, Jena Biosciences) in two cycles ...
-
bioRxiv - Biochemistry 2023Quote: The kinetic measurements for the linked complexes were performed on Fluoromax-4 (Horiba) where the intensity of fluorescence emission by mant-labelled GDP/GMPPNP (Jena Bioscience, Germany) at 440 nm was monitored after the excitation at 360 nm (protocol similar to the one used in Baranwal ...
-
bioRxiv - Microbiology 2021Quote: To verify the functionality of mutated SoGGDEF proteins they were tested for GTP binding using MANT-GTP (Jena Bioscience, Germany). Proteins were diluted to 1 μM and 100 μl of each sample was added in triplicates into a white round bottom 96-well plate before MANT-GTP with a final concentration of 1 μM was added ...
-
bioRxiv - Neuroscience 2022Quote: ... 250-300 μg of protein lysate together with 30 μl of 7-methyl GTP-agarose beads (Jena bioscience #AC-141) were incubated for 90 min at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... expression of protein was induced in 45 mL BHI medium supplemented with bleomycin and tetracyclin 10 mg/mL (Jena Bioscience). 5 ml of culture from nonclonal selection was added incubated for 72 h at 26°C with shaking at 100 rpm ...
-
bioRxiv - Microbiology 2023Quote: ... 20 μl of the prepared protein were mixed with 5 μl of 500-fold diluted JBS Thermofluor Dye (Jena Bioscience), prepared in the same buffer ...
-
bioRxiv - Biophysics 2021Quote: ... were polymerized from 4 mg/ml tubulin for 2 h at 37 °C in BRB80 supplemented with 1mM MgCl2 and 1mM GMPCPP (Jena Bioscience, NU-405). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Capturing of ATP binding proteins was performed on 75 μl of this mixture using 30 μl of Aminophenyl-ATP- or naked agarose beads (Jena Bioscience) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... GMPPNP binding Irgb6 crystals diffracting to 1.6 Å resolution were obtained from sitting drops with a 9 mg/ml protein solution containing 2 mM GMPPNP (Jena Bioscience) and a reservoir solution consisting of 0.1 M Sodium citrate buffer pH 5.6 ...
-
bioRxiv - Physiology 2022Quote: ... glycoproteins were enriched using Concanavalin A and azido-modifed proteins were immobilized on magnetic DBCO-beads (Jena Bioscience #CLK-1037-1) via click chemistry ...
-
bioRxiv - Microbiology 2023Quote: ... Protein-containing fractions were pooled and incubated with an ATP-agarose resin (Immobilized γ-Aminophenyl-ATP C10-spacer from Jena Bioscience) overnight at 4 °C with gentle agitation ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were measured using BCA and 300 µg of protein lysate was added to 50 µl of m7GTP beads (Jena Bioscience) for 2h at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... The chamber was pre-equilibrated with an oxygen scavenger cocktail 32 in 20 mM HEPES pH 7.4 with 150 mM NaCl and time-lapse images were acquired while flowing desired proteins in the same buffer in the absence or presence of 1 mM GTP (Jena Bioscience) and 1 mM MgCl2.