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Citations for Jena Bioscience :
151 - 176 of 176 citations for 8 4 Chlorophenylthio 2' O methyladenosine 3' 5' cyclic monophosphate sodium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The cells were then washed again 3x in 1xPBS and then incubated with 5 µM DBCO-488 (Jena Bioscience, CLK-1278-1) at 37 °C for 30 min or 5 µM Click-IT Alexa Fluor® 488 DIBO alkyne dye (ThermoScientific ...
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bioRxiv - Biochemistry 2023Quote: Nucleotide binding was analyzed using the non-hydrolysable GTP analogue N- methylanthraniloyl guanosine-5’-[(β,γ)-imido]triphosphate (mant-GMPPNP, Jena Bioscience). Varying protein concentrations of non-farnesylated GBP1 ranging from 0.02 µM to 40 µM were prepared in a 96-well plate in 50 mM Tris-HCl pH 7.9 ...
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bioRxiv - Biochemistry 2023Quote: In vitro transcribed RNase E 5’ UTR (4.5 µM) was incubated with 33 µM of pCp Cy5 (Jena Bioscience #NU-1706-CY5) in a total reaction volume of 100 µL consisting of 50 units of T4 RNA ligase 1 (NEB #M0204S) ...
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bioRxiv - Immunology 2021Quote: ... Azide-coupled Nbs were labeled by SPAAC (strain-promoted azide-alkyne cycloaddition) click chemistry reaction with 2-fold molar excess of DBCO-Cy5.5 (Jena Bioscience) for 2 h at 25°C ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then incubated with a 2–fold excess of ε–ATP or ε–ADP (Jena Bioscience, Jena, Germany) for two hours on ice ...
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bioRxiv - Biophysics 2020Quote: ... as a function of load in the presence of the non-hydrolyzable ATP analog adenosine-5’-[(β, γ)-imido] triphosphate (AMPPNP, Jena Bioscience). With AMPPNP ...
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bioRxiv - Cell Biology 2020Quote: ... from a mixture of unlabeled and HiLyte647-labeled tubulin (5:1, total tubulin concentration 100 μM) and 1 mM GMPCPP (Jena Bioscience, Jena, Germany) incubated at 37°C for 20 min ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and 40 cycles of PCR with either the sense or anti-sense primer in a Taq polymerase PCR mixture to which 0.067 mM of Digoxigenin-5-aminoallyl-dUTP (Jena Bioscience GmbH, Jena, Germany) had been added and in which the concentration of dTTP had been lowered to 0.133 mM ...
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bioRxiv - Cell Biology 2021Quote: ... Biotin and Alexa-Fluor-633-labeled porcine tubulin were generated in the laboratory as described (Al-Bassam, 2014) and were polymerized using the non-hydrolysable GTP analog guanosine-5’-[(α,β)-methyleno] triphosphate (termed GMPCPP; Jena Biosciences) or using the MT stabilizing drug ...
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bioRxiv - Biophysics 2019Quote: ... Biotin and Alexa-Fluor-633-labeled porcine tubulin were generated in the laboratory as described (Al-Bassam, 2014) and were polymerized using the non-hydrolyzable GTP analog guanosine-5’-[(α,β)-methyleno] triphosphate (termed GMPCPP; Jena Biosciences) or using the MT stabilizing drug ...
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bioRxiv - Plant Biology 2022Quote: ... The adenosine-5’-[(β,γ)-methyleno]triphosphate (APPCP) complex was prepared by soaking crystals in crystallization buffer supplemented with 10 mM APPCP (Jena Biosciences), 10 mM uridine and 10 mM MgCl2 for 3 min ...
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bioRxiv - Pathology 2022Quote: ... extracts were treated with 6μL/100μL of extract of a premix solution containing: 60μM of TAMRA-biotin-azide (Jena Bioscience, catalog number CLK-1048-5), 1mM CuSO4 in water (freshly prepared) ...
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bioRxiv - Cell Biology 2021Quote: ... Azide-coupled Nbs were then labelled by SPAAC (strain- promoted azide-alkyne cycloaddition) click chemistry reaction with 2-fold molar excess of Alexa-Fluor conjugated dibenzocyclooctyne (DBCO-AF647, Jena Bioscience) for 2 h at 25 °C ...
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bioRxiv - Molecular Biology 2022Quote: All samples were run in technical triplicates using the following PCR program (Table 2) on a qTOWER3/G cycler (Jena Biosciences).
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bioRxiv - Molecular Biology 2022Quote: ... Supernatants from the centrifugation of the disrupted cells were incubated with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) agarose (Jena Bioscience) on ice for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclear run-on (NRO) assays were performed with 2 biotin-11-NTPs (biotin-11-CTP and biotin-11-UTP, Jena Bioscience) as described in D ...
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bioRxiv - Microbiology 2021Quote: ... GMPPNP binding Irgb6 crystals diffracting to 1.6 Å resolution were obtained from sitting drops with a 9 mg/ml protein solution containing 2 mM GMPPNP (Jena Bioscience) and a reservoir solution consisting of 0.1 M Sodium citrate buffer pH 5.6 ...
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bioRxiv - Immunology 2023Quote: ... the SPAAC (strain-promoted azide-alkyne cycloaddition) click chemistry reaction was employed by incubating azide-coupled Nbs with 2-fold molar excess of DBCO-AF647 (Jena Bioscience) for 2 h at 25°C ...
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bioRxiv - Physiology 2023Quote: ... and cDNA was synthesised from 2 µg of RNA by reverse transcription (SCRIPT cDNA Synthesis Kit, Jena Bioscience GmBH, Jena, Germany). Gene expression assays for Il1b (Rn00580432_m1) ...
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bioRxiv - Cell Biology 2024Quote: ... were polymerized from 4 mg/ml tubulin for 2 h at 37°C in BRB80 supplemented with 1mM MgCl2 and 1mM GMPCPP (#NU-405, Jena Bioscience). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter) ...
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bioRxiv - Cell Biology 2020Quote: ... The solubilised proteins in the supernatant were used for the click reaction in a final volume of 120 μl (0.2% w/v final SDS concentration) with the following components added in order to the indicated final concentrations: 20 μM picolyl-biotin-azide (Jena Bioscience CLK-1167-5), 1.2 mM BTTAA (Jena Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... the thawed cryo-sections were subjected to a CuAAC reaction (as described above) using 5 mM biotin-azide in DMSO (> 95% purity, Jena Bioscience GmbH, Jena, Germany) instead of Azide-Fluor 488 ...
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bioRxiv - Biophysics 2021Quote: Tubulin polymerization kinetics were measured for concentrations ranging betwee 0.25 µM and 2 µM were polymerized by incubation with BRB-80 buffer containing 10% glycerol and either 1 mM GTP (Jena Bioscience, Germany) with 1 mM MgCl2 or 10 µM taxol ...
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bioRxiv - Cell Biology 2021Quote: ... kept for 2 h in DMEM to remove remaining TCO*K and were labeled with 1 µM Tet-Cy3 (Jena Bioscience, CLK-014-05) or Tet-BDP-FL (Jena Bioscience ...
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bioRxiv - Molecular Biology 2022Quote: ... single crystals were then soaked for 2 hours at 18°C in a solution containing 1 mM (Ta6Br12)2+ cluster (JBS Tantalum Cluster Derivatization Kit from Jena Bioscience GmbH, Jena, Germany). 25% glycerol cryo-protected native crystals or 50/50 paratone/paraffin oil mixture cryo-protected derivative crystals were flash-frozen in liquid nitrogen.
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bioRxiv - Genetics 2021Quote: ... or under amino acid-selective conditions in complete supplemental medium (CSM) (Difco yeast nitrogen extract without amino acids, amino acid powder from Sunrise Science Products, 2% μg/ml nourseothricin (Jena Bioscience, Jena, Germany) and 2 mM methionine was used to select strains containing pBV65 vector derivatives (42) ...