Labshake search
Citations for Jena Bioscience :
51 - 100 of 187 citations for 7 Bromo 2 chloromethyl 4H pyrido 1 2 a pyrimidin 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... Nuclear run-on (NRO) assays were performed with 2 biotin-11-NTPs (biotin-11-CTP and biotin-11-UTP, Jena Bioscience) as described in D ...
-
bioRxiv - Microbiology 2021Quote: ... GMPPNP binding Irgb6 crystals diffracting to 1.6 Å resolution were obtained from sitting drops with a 9 mg/ml protein solution containing 2 mM GMPPNP (Jena Bioscience) and a reservoir solution consisting of 0.1 M Sodium citrate buffer pH 5.6 ...
-
bioRxiv - Immunology 2023Quote: ... the SPAAC (strain-promoted azide-alkyne cycloaddition) click chemistry reaction was employed by incubating azide-coupled Nbs with 2-fold molar excess of DBCO-AF647 (Jena Bioscience) for 2 h at 25°C ...
-
bioRxiv - Physiology 2023Quote: ... and cDNA was synthesised from 2 µg of RNA by reverse transcription (SCRIPT cDNA Synthesis Kit, Jena Bioscience GmBH, Jena, Germany). Gene expression assays for Il1b (Rn00580432_m1) ...
-
bioRxiv - Cell Biology 2024Quote: ... were polymerized from 4 mg/ml tubulin for 2 h at 37°C in BRB80 supplemented with 1mM MgCl2 and 1mM GMPCPP (#NU-405, Jena Bioscience). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter) ...
-
bioRxiv - Molecular Biology 2019Quote: ... and incubated for 2 hours at 37°C in the absence or presence of N6-methyladenosine (Jena Biosciences, Cat no. NU-1101L) to generate ‘body-labeled’ m6A-RNAs ...
-
bioRxiv - Biochemistry 2022Quote: Guanine nucleotide exchange activity was measured by following changes in the fluorescence of the GDP derivative mant-dGDP [2’-Deoxy-3’-O-(N-Methyl-anthraniloyl) guanosine-5’-diphosphate sodium salt (Jena Bioscience GmbH)] (Margarit et al. ...
-
bioRxiv - Biophysics 2022Quote: ... The 2’-deoxy-ATP labeled with N-Methylanthraniloyl at the 3’-ribose position (mantATP) was also purchased from Jena Biosciences (10 mM stock). ATP and ADP were prepared from powder (MilliporeSigma ...
-
bioRxiv - Zoology 2020Quote: ... in which a third of the dTTP had been replaced with Digoxigenin-X-(5-aminoallyl)-2’-deoxyuridine-5’-triphosphate (Jena Bioscience, Jena, Germany) for 40 cycles ...
-
bioRxiv - Molecular Biology 2022Quote: ... USA (Catalog No. S3190) and 7-Methyl-guanosine-5’-monophosphate (m7GMP) was purchased from Jena Bioscience, Jena ...
-
bioRxiv - Genetics 2021Quote: ... or under amino acid-selective conditions in complete supplemental medium (CSM) (Difco yeast nitrogen extract without amino acids, amino acid powder from Sunrise Science Products, 2% μg/ml nourseothricin (Jena Bioscience, Jena, Germany) and 2 mM methionine was used to select strains containing pBV65 vector derivatives (42) ...
-
bioRxiv - Molecular Biology 2019Quote: ... 4-thio-UTP (4SU, Jena Bioscience) or 6-thio-GTP (6SG ...
-
bioRxiv - Biochemistry 2022Quote: ... in the presence of [α-32P]UTP and 7 mM “anti-reverse” cap analog (m7,3’-OGpppG; Jena Bioscience). All radiolabeled RNAs were gel-purified ...
-
bioRxiv - Microbiology 2024Quote: ... The following primary antibodies were diluted 1:400 in blocking buffer and incubated over night at 4°C: mouse anti-dsRNA J2 (RNT-SCI-10010200; Jena bioscience), mouse anti-DDX5 (67025 Proteintech ...
-
bioRxiv - Biophysics 2021Quote: ... GMPCPP seeds were prepared by polymerizing a 1:4 molar ratio of TAMRA labelled:unlabelled tubulin in the presence of guanosine-5’-[(α, β)-methyleno]triphosphate (GMPCPP, Jena Biosciences) in two cycles ...
-
bioRxiv - Microbiology 2019Quote: ... At least 1000 µg of the cell lysates were incubated with Sepharose beads coupled to 7-methylguanosine (m7GTP, Jena Biosciences). Input cell lysates were collected for western blot analysis while the remaining were incubated overnight with continuous rotation at 4°C ...
-
bioRxiv - Neuroscience 2022Quote: ... 250-300 μg of protein lysate together with 30 μl of 7-methyl GTP-agarose beads (Jena bioscience #AC-141) were incubated for 90 min at 4°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... One half was subjected to a click chemistry reaction with disulfo-Cy5-picolyl-azide (Jena Bioscience CLK-1177) for one hour ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell lysates (500 mg of protein) were incubated with 50 μl of 7-methyl-GTP-Agarose slurry (Jena Biosciences, AC-155S) for 16 h at 4 °C ...
-
bioRxiv - Immunology 2020Quote: ... and 4 mM of each ribonucleotide triphosphate (Jena Bioscience) and incubated at 37 °C overnight ...
-
bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified with 4% Glutathione Agarose (Jena Biosciences) according to the manufacturer’s instructions ...
-
Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Dried lipid films were click reacted in a 40 μl reaction mix containing 0.45 mM fluorogenic dye 3-azido-7-hydroxycoumarin (Jena Bioscience, CLK-FA047), 1.4 mM Cu(I)tetra(acetonitrile ...
-
bioRxiv - Biophysics 2023Quote: ... 50 mM each of Azide-(PEG)4-NHS (Jena Biosciences GmbH, Jena, Germany) and Methyl-(PEG)4-NHS (Life technologies ...
-
bioRxiv - Biophysics 2024Quote: ... 50 mM each of azide-(PEG)4-NHS (Jena Biosciences GmbH, Jena, Germany) and methyl-(PEG)4-NHS (Life technologies ...
-
bioRxiv - Biophysics 2022Quote: ... The 4 bp overhangs formed were filled in with biotin-11-dGTP (Jena Bioscience), biotin-16-dUTP (Roche) ...
-
bioRxiv - Neuroscience 2022Quote: ... 0.4 μM 5/6- TAMRA-PEG 4 -Alkyne (Jena Bioscience; Jena, Germany; CLK-TA108) and 0.2 mM freshly prepared CuSO4 in 1x PBS pH7.8 ...
-
bioRxiv - Biochemistry 2020Quote: ... ∼900 μL of 4 μM forked DNA duplex and 100 μM ATPγS (Jena Bioscience, #NU-406-50) in Buffer S was applied to the column at 0.05 mL/min ...
-
bioRxiv - Molecular Biology 2021Quote: ... 4 samples each) were extracted with CHAPS Lysis buffer from the Click Chemistry Capture Kit (Jena Bioscience). Input cell numbers were adjusted to give similar amount of total injected peptides ...
-
bioRxiv - Molecular Biology 2021Quote: ... 500 μg of protein was incubated overnight at 4°C with m7GTP agarose beads (Jena biosciences, #AC-155S). Beads were washed with cap binding buffer and suspended in 20 μL Laemmli buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... 4 mM DMSO was used to dissolve 25 mM dibenzylcyclooctyne NHS (DBCO-NHS) ester purchased from Jena Bioscience. The DBCO-NHS ester was 33.3-fold molar excess over antibodies ...
-
bioRxiv - Immunology 2023Quote: ... they were injected intraperitoneally with 1.5mg L-AHA (Thermo) and then 4 hours later 1mg OPP (Jena Bioscience) dissolved in 100μl PBS ...
-
bioRxiv - Biophysics 2023Quote: ... we ran PCR reactions with added biotin-16-dUTP or 5-DBCO-(PEG)4-dUTP (Jena Biosciences GmbH, Jena, Germany), respectively ...
-
bioRxiv - Biochemistry 2023Quote: The kinetic measurements for the linked complexes were performed on Fluoromax-4 (Horiba) where the intensity of fluorescence emission by mant-labelled GDP/GMPPNP (Jena Bioscience, Germany) at 440 nm was monitored after the excitation at 360 nm (protocol similar to the one used in Baranwal ...
-
bioRxiv - Biochemistry 2023Quote: ... 0.9 mL of DNase solution (15 mM MgSO4, 1 mM DTT and 1 mM PMSF, 1 x Turbo DNase (Jena biosciences)) was added and the samples were allowed to shake at 37 °C for 10 min before transferring to ice ...
-
bioRxiv - Cell Biology 2019Quote: ... 12 μM unlabeled tubulin + 1 μM Alexa-568 tubulin + 1 μM biotin tubulin was polymerized in BRB80 (80 mM Pipes, 1 mM EGTA, 1 mM MgCl2) in the presence of 1 mM GMPCPP (Jena Bioscience, NU-405L) for 1 h at 37°C ...
-
bioRxiv - Cell Biology 2023Quote: ... as in 74 from a mixture of unlabeled, rhodamine- and biotin-labeled tubulins (8:1:1, total tubulin concentration 5 μM) supplemented with 1 mM GMPCPP (Jena Bioscience, NU-405L). A flow chamber was prepared as for assays with taxol-stabilized microtubules ...
-
bioRxiv - Biochemistry 2023Quote: ... 1 mM GTP (Roth, K056.4) or 1 mM GMP (Jena Biosciences, JBS-NU-1028), was added to the washed beads and incubated gently by rocking for 30 min at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 mM GMPCPP (Jena Biosciences) and 4 mM MgCl2 ...
-
bioRxiv - Microbiology 2023Quote: ... cultures (with an OD600 of approximately 0.3) were pulse-labeled with 250 µM 4-azido-L-homoalanine (AHA, Jena Bioscience, Cat. No. CLK-AA005) and incubated for 15 min at 37 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GMPCPP (Jena Bioscience, Jena, Germany). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter ...
-
bioRxiv - Cell Biology 2020Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GMPCPP (Jena Bioscience, Jena, Germany). The polymerized microtubules were centrifuged for 30 min at 18.000 x g in a Microfuge 18 Centrifuge (Beckman Coulter ...
-
bioRxiv - Neuroscience 2024Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GTP (Jena Bioscience, Jena, Germany) for 30 minutes at 37 °C prior to centrifugation at 18.000 x g for 30 min in a Microfuge 18 Centrifuge (Beckman Coulter ...
-
bioRxiv - Biophysics 2021Quote: ... 1 mM GMPCPP (Jena Bioscience, Germany), 1 mM MgCl2 and 4 mg mL-1 of porcine brain tubulin ...
-
bioRxiv - Cell Biology 2021Quote: ... with 1 mM GMPCPP (Jena Bioscience) in PEM80 (80 mM Pipes ...
-
bioRxiv - Biochemistry 2023Quote: ... and 1 mM GTP (Jena Bioscience). A 12.5 µM solution of fluorescently labeled tubulin in 1x assay buffer containing an oxygen scavenger system (freshly mixed 0.035 mg/ml catalase (Sigma Aldrich) ...
-
bioRxiv - Biochemistry 2020Quote: ... AF488-Azide (#CLK-1275-1, Jena Bioscience) was used to test success of bead – DBCO coupling ...
-
bioRxiv - Biochemistry 2023Quote: ... 1 mM GMPPCP (NU-405S, Jena Bioscience) or 1 mM BeF4 (prepared by mixing 50 mM BeSO4 and NaF 100 mM to make 10 mM BeF3) ...
-
bioRxiv - Microbiology 2021Quote: ... Labeling was achieved by using a 1:1 molar ratio of dCTP:Biotin-11-dCTP (NU-809-BIOX, Jena Bioscience) in the dNTPs used for the PCR (GoTaq DNA polymerase ...
-
bioRxiv - Microbiology 2023Quote: ... and pellets were resuspended using 1/5 or 1/10 dilutions of detergents stocks from the JBScreen (Jena Bioscience) or Detergent Screen (Hampton) ...
-
bioRxiv - Cell Biology 2022Quote: ... was incubated with 1 mM GMPCPP (Jena Biosciences) at 37°C for 30 minutes ...