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Citations for Jena Bioscience :
51 - 100 of 107 citations for 6 Chloro 5 methylisatin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were pulsed with OPP (20μM, Jena Bioscience, cat: NU-931-5) in complete RPMI supplemented with the cytoplasmic translation inhibitor harringtonine (1μM ...
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bioRxiv - Cancer Biology 2023Quote: EDA-m7GTP-5-FAM (NU-824-5FM) was purchased from Jena Bioscience and used as received ...
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bioRxiv - Biophysics 2019Quote: ... Cy3 labeled 2’/3’-O-(2-aminoethyl-carbamoyl)-adenosine-5’-triphosphate (Jena Bioscience) was added into the imaging buffer instead of ATP.
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bioRxiv - Cell Biology 2023Quote: ... followed by 30 min incubation with 50μM OPP (Jena Bioscience, NU-913-5). Cultures were then washed with room temperature PBS and fixed with 4 % of PFA ...
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bioRxiv - Cell Biology 2023Quote: ... This media was changed and ATP (5 mM) (Jena Biosciences cat. NU-1010) was added for 24 h prior to collection of conditioned media ...
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bioRxiv - Cell Biology 2023Quote: ... This media was changed and ATP (5 mM) (Jena Biosciences cat. NU-1010) was added for 24 h prior to collection of conditioned media ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM of each triphosphate ribonucleotide (standard nucleotides were purchased from Jena Bioscience GmBH and pseudouridine and N1-methylpseudouridine from BOC sciences) ...
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bioRxiv - Neuroscience 2020Quote: ... AMP-CPP (α,β-methyleneadenosine 5’-triphosphate lithium salt, Jena Bioscience #NU-421-25), AMP-PCP (β,γ-Methyleneadenosine 5′-triphosphate disodium salt ...
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bioRxiv - Plant Biology 2023Quote: ... DNA oligonucleotides used as probes were extended with 5-Azido-PEG4-dCTP (Jena Bioscience) and terminal transferase (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... All exchange experiments reported in this article were initiated by incubation of G-actin (1 µM) with N6-(6-Amino)hexyl-ATP-ATTO-488 (0.1 µM; Jena Bioscience, Germany, ref. NU-805-488) in G+ME buffer (5mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 % of UTP (resp. GTP) was substituted with either 5-ethynyl-UTP (5EU, Jena Bioscience), 5-bromo-UTP (5BrU ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 pmols of RNA were incubated with 150 pmoles of pCp-Cy-5 (Jena Biosciences), 20 units of T4-RNA ligase (NEB) ...
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Tail Length and E525K Dilated Cardiomyopathy Mutant Alter Human β-Cardiac Myosin Super-Relaxed StatebioRxiv - Molecular Biology 2023Quote: The fluorescence of 3’-O-(N-Methyl-anthraniloyl)-2’-deoxyadenosine-5’-triphosphate (mantATP) (Jena Biosciences) was measured with 290 nm excitation and a 395 nm long-pass emission filter in a stopped-flow apparatus (Applied Photophysics ...
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bioRxiv - Biochemistry 2020Quote: ... 3’dGTP and cytidine-5’-[(α,β)-methyleno]triphosphate (CMPCPP) were from Jena Bioscience (Jena, Germany); 2’dGTP ...
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bioRxiv - Cell Biology 2021Quote: ... Cells migrating in microfluidic devices were pulsed with 1 mM 5-ethynyl uridine (EU, Jena Bioscience) in complete DMEM for 4 hours ...
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bioRxiv - Plant Biology 2021Quote: ... The in vitro transcription reaction contained 0.25 mM 5-Azido-C3-UTP (Jena Bioscience, Jena, Germany). Purified RNA probes were Click-labelled with either Cy5.5-alkyne or Cy7.5-alkyne (Lumiprobe ...
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bioRxiv - Biophysics 2021Quote: ... was amplified by PCR in the presence of 1:5 Digoxigenin-11-dUTP:dTTP (Jena Bioscience, Germany) using primers CD21 and CD26 (Supplementary Table 1) ...
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bioRxiv - Molecular Biology 2022Quote: ... USA (Catalog No. S3190) and 7-Methyl-guanosine-5’-monophosphate (m7GMP) was purchased from Jena Bioscience, Jena ...
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bioRxiv - Genomics 2023Quote: ... instead of UTP or N4-Acetyl-cytidine-5’-triphosphate (N4-Acetyl-CTP, Jena Bioscience, NU-988S) instead of CTP ...
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bioRxiv - Cell Biology 2023Quote: ... Guanosine-5’-[(α,β)-methyleno] triphosphate (GMPCPP) was purchased from Jena Bioscience (NU-405S, Jena, Germany,). Glutathione Sepharose 4B beads (17075605 ...
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bioRxiv - Cell Biology 2023Quote: ... Guanosine-5’-[(α,β)-methyleno] triphosphate (GMPCPP) was purchased from Jena Bioscience (NU-405S, Jena, Gemany). Taxol was purchased from Cytoskeleton (TXD01 ...
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bioRxiv - Biophysics 2023Quote: ... pH 7.4) with 1 mM guanosine-5’-[(α,β)-methyleno]triphosphate (GMPCPP; NU-405S, Jena Bioscience) and the solution was incubated for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were injected with either 50 mM or 250 mM 5-ethynyl uridine (Jena Biosciences, Jena, Germany) using a PLI-100 Plus Pico-Injector (Harvard Apparatus ...
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bioRxiv - Biophysics 2020Quote: ... pH = 6.9) with 1mM MgCl2 and 1mM guanosine-5’-[(α,β)-methyleno] triphosphate (GMPCPP, Jena Bioscience, Jena, Germany) for 1.5 h at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells were incubated with pre-warmed fresh media with 400 µM 5-ethynyl uridine (5EU, Jena Biosciences) for 1 ...
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bioRxiv - Biochemistry 2022Quote: ... eluted C3-SC1TECs were pre-warmed at 37 °C for 2 minutes before 18.75 μl of C3-SC1TECs were mixed with 6.25 μl of pre-warmed Chase Mix G (2.5 μl 5 mM NTPs, 2.5 μl 100 μg/ml rifampicin, 1.25 μl of ppGpp (Jena Bioscience) in nuclease-free water at 20X final ppGpp concentration) ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... Doubly stabilized MTs were polymerized by incubating 5 µM tubulin with 0.5 mM GMPCPP (Jena BioScience, #NU-405L) for 20 min at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... then 5×106 cells were plated on ESAW agar plates containing 450 μg mL-1 nourseothricin (Jena Bioscience), and incubated in constant light at 18 °C.
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bioRxiv - Genetics 2021Quote: ... the PCR labelling Cy-3 and Cy-5 kits (Jena Bioscience, ref# PP-301L-Cy3 and #APP-101-Cy5) according to the manufacturer’s instructions with M13 universal primers ...
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bioRxiv - Microbiology 2020Quote: ... For phasing the crystals were soaked overnight in 50 mM 5-amino-2,4,6-triiodoisophthalic acid (I3C)/LiOH (Magic Triangle, Jena Biosciences)42 in cryobuffer ...
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bioRxiv - Microbiology 2023Quote: ... and pellets were resuspended using 1/5 or 1/10 dilutions of detergents stocks from the JBScreen (Jena Bioscience) or Detergent Screen (Hampton) ...
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bioRxiv - Plant Biology 2023Quote: ... RNA probes were prepared by in vitro transcription in the presence of 5-azido-C3-UTP nucleotides (Jena Biosciences) with final linking to sulpho-cyanine cy5.5 or cy7.5 dye (Lumiprobe ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown on coverslips and transcription levels were measured by pulse labeling with 5’ethynyl uridine (EU, Jena Bioscience) in Ham’s F10 medium supplemented with 10% dialyzed FCS and 20 mM HEPES buffer (both Gibco) ...
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bioRxiv - Biophysics 2020Quote: ... 5’-TAGTCCAGAACGAGACCGCAACAGCACAACCCAAACTG-3’ and 5’-AATCTGCTGCAATGCCACAG-3’ (underline section indicates overhang) with a 10:1 ratio of dTTP:digoxigenin-11-dUTP (Jena Bioscience). The labelled fragments were purified using a PCR purification kit (QIAquick ...
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bioRxiv - Biochemistry 2021Quote: ... cells were labelled for 20 minutes with EU (5-ethynyl uridine, Jena Bioscience CLK-N002, final concentration at 1 mM). Harvested cells were firstly labeled with Zombie AquaTM (BioLegend ...
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bioRxiv - Biochemistry 2021Quote: ... RNAs were fluorescently labeled with cyanine 5 at the 3’ end with the help of pCp-Cy5 (Jena Bioscience, 84179) and T4 RNA ligase I (NEB ...
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bioRxiv - Microbiology 2020Quote: ... Genome-labeled AdV was produced by growing the virus in A549 cells in the presence of 2.5 μM EdC (5-ethynyl-2’-deoxycytidine, Jena Biosciences) as described 52 ...
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bioRxiv - Pathology 2023Quote: Nascent RNA was labeled by intraperitoneal injection of 88 mg/kg 5-ethynyl-uridine (EU) (Jena Biosciences, #CLK-N002-10) to P25 mice ...
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bioRxiv - Biophysics 2023Quote: ... we ran PCR reactions with added biotin-16-dUTP or 5-DBCO-(PEG)4-dUTP (Jena Biosciences GmbH, Jena, Germany), respectively ...
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bioRxiv - Microbiology 2023Quote: ... 20 μl of the prepared protein were mixed with 5 μl of 500-fold diluted JBS Thermofluor Dye (Jena Bioscience), prepared in the same buffer ...
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bioRxiv - Plant Biology 2022Quote: For dsRNA labeling with the fluorochrome we followed the same dsRNA synthesis method described above but including fluorescein-X-(5-aminoallyl)-UTP (Jena Bioscience) in the reaction mix following manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... GMPCPP seeds were prepared by polymerizing a 1:4 molar ratio of TAMRA labelled:unlabelled tubulin in the presence of guanosine-5’-[(α, β)-methyleno]triphosphate (GMPCPP, Jena Biosciences) in two cycles ...
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bioRxiv - Molecular Biology 2021Quote: ... The 100% m6A modified full-length PAN transcript was synthesized using the MEGAscript T7 Transcription Kit following the manufacturer’s protocol but substituting adenosine-triphosphate with N6-methyladenosine-5’-triphosphate (Jena Bioscience NU-1101S). RNAs were purified using the MEGAclear Transcription Clean-Up Kit (ThermoFisher AM1908).
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bioRxiv - Microbiology 2020Quote: ... The cells were then washed again 3x in 1xPBS and then incubated with 5 µM DBCO-488 (Jena Bioscience, CLK-1278-1) at 37 °C for 30 min or 5 µM Click-IT Alexa Fluor® 488 DIBO alkyne dye (ThermoScientific ...
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bioRxiv - Biochemistry 2022Quote: Guanine nucleotide exchange activity was measured by following changes in the fluorescence of the GDP derivative mant-dGDP [2’-Deoxy-3’-O-(N-Methyl-anthraniloyl) guanosine-5’-diphosphate sodium salt (Jena Bioscience GmbH)] (Margarit et al. ...
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bioRxiv - Biochemistry 2023Quote: Nucleotide binding was analyzed using the non-hydrolysable GTP analogue N- methylanthraniloyl guanosine-5’-[(β,γ)-imido]triphosphate (mant-GMPPNP, Jena Bioscience). Varying protein concentrations of non-farnesylated GBP1 ranging from 0.02 µM to 40 µM were prepared in a 96-well plate in 50 mM Tris-HCl pH 7.9 ...
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bioRxiv - Biochemistry 2023Quote: In vitro transcribed RNase E 5’ UTR (4.5 µM) was incubated with 33 µM of pCp Cy5 (Jena Bioscience #NU-1706-CY5) in a total reaction volume of 100 µL consisting of 50 units of T4 RNA ligase 1 (NEB #M0204S) ...
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bioRxiv - Biophysics 2020Quote: ... as a function of load in the presence of the non-hydrolyzable ATP analog adenosine-5’-[(β, γ)-imido] triphosphate (AMPPNP, Jena Bioscience). With AMPPNP ...
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bioRxiv - Cell Biology 2020Quote: ... from a mixture of unlabeled and HiLyte647-labeled tubulin (5:1, total tubulin concentration 100 μM) and 1 mM GMPCPP (Jena Bioscience, Jena, Germany) incubated at 37°C for 20 min ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and 40 cycles of PCR with either the sense or anti-sense primer in a Taq polymerase PCR mixture to which 0.067 mM of Digoxigenin-5-aminoallyl-dUTP (Jena Bioscience GmbH, Jena, Germany) had been added and in which the concentration of dTTP had been lowered to 0.133 mM ...