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Citations for Jena Bioscience :
151 - 189 of 189 citations for 6 1 Aminoethyl 2H 1 4 benzoxazin 3 4H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Then, 0.9 ml of turbo DNase solution (15 mM MgSO4, 10 µg ml−1 turbo DNase (Jena Bioscience, EN-180), 1 mM TCEP and 0.5 mM phenylmethanesulfonyl fluoride ...
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bioRxiv - Immunology 2021Quote: ... XCL1-K(N3) and sdAb-K(N3) were subsequently reacted with DBCO-Cy5.5 (3 equiv.) (CLK-1046, Jena BioSciences) overnight at RT on an end-over-end shaker ...
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bioRxiv - Genetics 2021Quote: ... the PCR labelling Cy-3 and Cy-5 kits (Jena Bioscience, ref# PP-301L-Cy3 and #APP-101-Cy5) according to the manufacturer’s instructions with M13 universal primers ...
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bioRxiv - Biophysics 2021Quote: ... MTs were prepared either with 0.75 µM mung tubulin or 30 µM goat brain tubulin in BRB-80 buffer with 1 mM GMPCPP (Jena Bioscience, Germany) and incubated at 37°C for 60 min ...
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bioRxiv - Microbiology 2020Quote: ... The assay was carried out at 37°C and the reaction was started by addition of 1 mM pppGpp (Jena Bioscience), and samples for the nucleotide measurement were taken after 15 min ...
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bioRxiv - Biochemistry 2020Quote: ... Additives as indicated were added during the primary antibody incubation step at a final concentration of 1 µM for adenosine (Jena Bioscience), AMP (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... For synthesis of the 4sU-labeled spike-ins 1/10 of UTP in the transcription reactions was replaced with 4sUTP (Jena BioScience).
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bioRxiv - Molecular Biology 2019Quote: ... GMPCPP-stabilized seeds were made by two rounds of polymerization in 25 µM tubulin (40% dig-tubulin) supplemented with 1 mM GMPCPP (Jena Biosciences) as described (Volkov et al. ...
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bioRxiv - Physiology 2022Quote: ... glycoproteins were enriched using Concanavalin A and azido-modifed proteins were immobilized on magnetic DBCO-beads (Jena Bioscience #CLK-1037-1) via click chemistry ...
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bioRxiv - Microbiology 2023Quote: ... 500 ng of RNA was reverse-transcribed with gene specific primers (Supplementary information 1) using the SCRIPT cDNA Synthesis Kit (Jena Bioscience) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The chamber was pre-equilibrated with an oxygen scavenger cocktail 32 in 20 mM HEPES pH 7.4 with 150 mM NaCl and time-lapse images were acquired while flowing desired proteins in the same buffer in the absence or presence of 1 mM GTP (Jena Bioscience) and 1 mM MgCl2.
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bioRxiv - Biochemistry 2020Quote: ... ∼900 μL of 4 μM forked DNA duplex and 100 μM ATPγS (Jena Bioscience, #NU-406-50) in Buffer S was applied to the column at 0.05 mL/min ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 samples each) were extracted with CHAPS Lysis buffer from the Click Chemistry Capture Kit (Jena Bioscience). Input cell numbers were adjusted to give similar amount of total injected peptides ...
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bioRxiv - Biochemistry 2020Quote: Guanosine nucleotides labeled with fluorescent N-methyl-anthranoyl (mant) groups O-linked with 2’ or 3’ of ribose (Jena Biosciences), were used for binding studies ...
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bioRxiv - Biochemistry 2021Quote: ... RNAs were fluorescently labeled with cyanine 5 at the 3’ end with the help of pCp-Cy5 (Jena Bioscience, 84179) and T4 RNA ligase I (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... in the presence of 0.4μL 5-(3-Aminoallyl)-uridine-5’-triphosphate labeled with ATTO 680(Aminoallyl-UTP-ATTO-680, NU-821-680, Jena bioscience) in each reaction ...
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bioRxiv - Biophysics 2021Quote: Tubulin polymerization kinetics were measured for concentrations ranging betwee 0.25 µM and 2 µM were polymerized by incubation with BRB-80 buffer containing 10% glycerol and either 1 mM GTP (Jena Bioscience, Germany) with 1 mM MgCl2 or 10 µM taxol ...
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bioRxiv - Biochemistry 2021Quote: ... RHEB was preincubated for 1 h with a 30-fold molar excess of GTPγS (Jena Bioscience NU-412-20, lot IT008-18). Next ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then washed again 3x in 1xPBS and then incubated with 5 µM DBCO-488 (Jena Bioscience, CLK-1278-1) at 37 °C for 30 min or 5 µM Click-IT Alexa Fluor® 488 DIBO alkyne dye (ThermoScientific ...
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bioRxiv - Biochemistry 2023Quote: ... the sequence of corresponding constructs of proLEG were cloned into pLEXSY-sat2.1 vectors and transfected into LEXSY P10 host strain of Leishmania tarentolae cells of the LEXSYcon2.1 expression system (Jena Biosciences, Jena, Germany). The resulting constructs carried N-terminal His6-tags and an N-terminal signal sequence for secretion into the supernatant ...
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bioRxiv - Synthetic Biology 2023Quote: ... we added all four nucleotides at a final concentration of 1 µM (Biotin-7-dATP, Jena Biosciences, and dC-Atto647N or dU-Atto488, Jena Biosciences with respective pair of unmodified nucleotides ...
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bioRxiv - Developmental Biology 2023Quote: ... After two additional washes the Tn5 was added (1:100) in CUT&Tag med buffer (20 mM HEPES [pH 7.5] (Jena Bioscience, #CSS-511), 300 mM NaCl (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 μg of protein was incubated overnight at 4°C with m7GTP agarose beads (Jena biosciences, #AC-155S). Beads were washed with cap binding buffer and suspended in 20 μL Laemmli buffer ...
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bioRxiv - Biochemistry 2022Quote: ... 4 mM DMSO was used to dissolve 25 mM dibenzylcyclooctyne NHS (DBCO-NHS) ester purchased from Jena Bioscience. The DBCO-NHS ester was 33.3-fold molar excess over antibodies ...
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bioRxiv - Immunology 2023Quote: ... they were injected intraperitoneally with 1.5mg L-AHA (Thermo) and then 4 hours later 1mg OPP (Jena Bioscience) dissolved in 100μl PBS ...
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bioRxiv - Molecular Biology 2023Quote: T7 promoter containing double stranded DNA (Supplementary Table 3) was used as a template for in vitro transcription with HighYield T7 mRNA Synthesis Kit (ac4CTP) (Jena Bioscience).
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bioRxiv - Cell Biology 2019Quote: ... fluorescently labelled and non-labelled tubulin in a ratio 18:12:70 (total of 20 μM tubulin) with 1 mM GMPCPP (NU-405 Jena BioScience, Jena, Germany) at 37°C for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... and GTP or GDPCP were assembled in 1×10 buffer with 10 mM βME by preincubation of eEF1A and GTP (PromegaTM Corp., Madison, WI) or GDPCP (Jena Bioscience, Jena, Germany) for 5 minutes at 37°C followed by addition of tRNAAla or Ala-tRNAAla for 1 minute at 37°C and were then kept on ice until use ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Dried lipid films were click reacted in a 40 μl reaction mix containing 0.45 mM fluorogenic dye 3-azido-7-hydroxycoumarin (Jena Bioscience, CLK-FA047), 1.4 mM Cu(I)tetra(acetonitrile ...
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bioRxiv - Biochemistry 2022Quote: Guanine nucleotide exchange activity was measured by following changes in the fluorescence of the GDP derivative mant-dGDP [2’-Deoxy-3’-O-(N-Methyl-anthraniloyl) guanosine-5’-diphosphate sodium salt (Jena Bioscience GmbH)] (Margarit et al. ...
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bioRxiv - Evolutionary Biology 2024Quote: ... strains with MYO3 or MYO5 locus fused at its 3’ to the DHFR F[1,2] with a nourseothricin-resistance marker (NAT 100 μg/ml, Jena Bioscience GmbH, Germany). To insert the variant libraries instead of the wild-type SH3 domains ...
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bioRxiv - Cell Biology 2021Quote: ... kept for 2 h in DMEM to remove remaining TCO*K and were labeled with 1 µM Tet-Cy3 (Jena Bioscience, CLK-014-05) or Tet-BDP-FL (Jena Bioscience ...
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bioRxiv - Molecular Biology 2022Quote: ... single crystals were then soaked for 2 hours at 18°C in a solution containing 1 mM (Ta6Br12)2+ cluster (JBS Tantalum Cluster Derivatization Kit from Jena Bioscience GmbH, Jena, Germany). 25% glycerol cryo-protected native crystals or 50/50 paratone/paraffin oil mixture cryo-protected derivative crystals were flash-frozen in liquid nitrogen.
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bioRxiv - Biophysics 2023Quote: ... we ran PCR reactions with added biotin-16-dUTP or 5-DBCO-(PEG)4-dUTP (Jena Biosciences GmbH, Jena, Germany), respectively ...
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bioRxiv - Biophysics 2022Quote: ... The 2’-deoxy-ATP labeled with N-Methylanthraniloyl at the 3’-ribose position (mantATP) was also purchased from Jena Biosciences (10 mM stock). ATP and ADP were prepared from powder (MilliporeSigma ...
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bioRxiv - Biochemistry 2023Quote: The kinetic measurements for the linked complexes were performed on Fluoromax-4 (Horiba) where the intensity of fluorescence emission by mant-labelled GDP/GMPPNP (Jena Bioscience, Germany) at 440 nm was monitored after the excitation at 360 nm (protocol similar to the one used in Baranwal ...
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bioRxiv - Biophysics 2021Quote: ... were polymerized from 4 mg/ml tubulin for 2 h at 37 °C in BRB80 supplemented with 1mM MgCl2 and 1mM GMPCPP (Jena Bioscience, NU-405). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter) ...
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bioRxiv - Immunology 2024Quote: ... Immunostaining was performed in four steps to avoid unspecific binding of the secondary antibodies: i) incubation overnight at 4LJ with the mouse monoclonal IgG2a J2 antibody anti-dsRNA (Jena Bioscience, cat. RNT-SCI-10010500, dilution 1:200), ii ...
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bioRxiv - Microbiology 2023Quote: ... cultures (with an OD600 of approximately 0.3) were pulse-labeled with 250 µM 4-azido-L-homoalanine (AHA, Jena Bioscience, Cat. No. CLK-AA005) and incubated for 15 min at 37 °C ...