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Citations for Jena Bioscience :
51 - 100 of 194 citations for 6 1 2 4 TRIAZOL 1YL PURINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were labeled at the 3’ end using pCp-Cy5 (Cytidine-5’-phosphate-3’-(6-aminohexyl) phosphate) (Jena Biosciences). For each binding experiment ...
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bioRxiv - Biophysics 2019Quote: ... 2’-(3’)-bis-O-(N-methylanthraniloyl)-GDP (Jena Biosciences). Rab5 was loaded with mant-GDP in Rab5 buffer + 10mM EDTA and 5-fold excess mant-GDP ...
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bioRxiv - Microbiology 2023Quote: ... packed with 2 ml Ni-NTA agarose (Jena Bioscience) and equilibrated with 10 column volumes (CV ...
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bioRxiv - Immunology 2023Quote: ... 10 μM EdU (5-ethynyl-2’-deoxyuridine, Jena Bioscience) was added to the cell culture medium for 10 min at the end of 72 h culture on anti-CD3ε- and anti-CD28-coated cell culture plates ...
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bioRxiv - Biophysics 2023Quote: ... 50 mM each of Azide-(PEG)4-NHS (Jena Biosciences GmbH, Jena, Germany) and Methyl-(PEG)4-NHS (Life technologies ...
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bioRxiv - Biophysics 2024Quote: ... 50 mM each of azide-(PEG)4-NHS (Jena Biosciences GmbH, Jena, Germany) and methyl-(PEG)4-NHS (Life technologies ...
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 2 mM 3’-deoxyguanosine-5’-triphosphate (3’-dGTP, Jena Bioscience) and 4 mM MgCl2 ...
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... 2 mM 3’-deoxyadenosine-5’-triphosphate (3’-dATP, Jena Bioscience), 4 mM MgCl2 and 2 μM G-actin ...
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bioRxiv - Molecular Biology 2022Quote: ... short oligo DNAs for each target (see Supplementary Table 6) were pooled and labelled with 5-Propargylamino-ddUTP-Cy3 or -Cy5 (Jena Biosciences) using the Terminal Deoxynucleotidyl Transferase (Thermo Fisher)52 ...
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bioRxiv - Biophysics 2022Quote: ... The 4 bp overhangs formed were filled in with biotin-11-dGTP (Jena Bioscience), biotin-16-dUTP (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... 100 uL of alkyne agarose slurry (Jena Bioscience #CLK-1032-2) was washed with IP buffer (1% CHAPS ...
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bioRxiv - Biochemistry 2020Quote: ... ∼900 μL of 4 μM forked DNA duplex and 100 μM ATPγS (Jena Bioscience, #NU-406-50) in Buffer S was applied to the column at 0.05 mL/min ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 samples each) were extracted with CHAPS Lysis buffer from the Click Chemistry Capture Kit (Jena Bioscience). Input cell numbers were adjusted to give similar amount of total injected peptides ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 μg of protein was incubated overnight at 4°C with m7GTP agarose beads (Jena biosciences, #AC-155S). Beads were washed with cap binding buffer and suspended in 20 μL Laemmli buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were starved of methionine for one hour then 4-Azido-L-homoalanine HCl (L-AHA) (Jena Bioscience) was added to the media (final concentration 40μM ...
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bioRxiv - Biochemistry 2022Quote: ... 4 mM DMSO was used to dissolve 25 mM dibenzylcyclooctyne NHS (DBCO-NHS) ester purchased from Jena Bioscience. The DBCO-NHS ester was 33.3-fold molar excess over antibodies ...
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bioRxiv - Immunology 2023Quote: ... they were injected intraperitoneally with 1.5mg L-AHA (Thermo) and then 4 hours later 1mg OPP (Jena Bioscience) dissolved in 100μl PBS ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and incubated with 2 ml of Ni-NTA Agarose (Jena Bioscience) pre-equilibrated with the lysis buffer for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: All nucleoside triphosphates (NTPs) and 2’-deoxy nucleoside triphosphates (dNTPs) were purchased from Jena Biosciences. Chemical and reagents used in synthesis were obtained from Merck ...
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Tail Length and E525K Dilated Cardiomyopathy Mutant Alter Human β-Cardiac Myosin Super-Relaxed StatebioRxiv - Molecular Biology 2023Quote: The fluorescence of 3’-O-(N-Methyl-anthraniloyl)-2’-deoxyadenosine-5’-triphosphate (mantATP) (Jena Biosciences) was measured with 290 nm excitation and a 395 nm long-pass emission filter in a stopped-flow apparatus (Applied Photophysics ...
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bioRxiv - Biophysics 2023Quote: ... we ran PCR reactions with added biotin-16-dUTP or 5-DBCO-(PEG)4-dUTP (Jena Biosciences GmbH, Jena, Germany), respectively ...
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bioRxiv - Biochemistry 2023Quote: The kinetic measurements for the linked complexes were performed on Fluoromax-4 (Horiba) where the intensity of fluorescence emission by mant-labelled GDP/GMPPNP (Jena Bioscience, Germany) at 440 nm was monitored after the excitation at 360 nm (protocol similar to the one used in Baranwal ...
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bioRxiv - Biochemistry 2023Quote: ... 0.9 mL of DNase solution (15 mM MgSO4, 1 mM DTT and 1 mM PMSF, 1 x Turbo DNase (Jena biosciences)) was added and the samples were allowed to shake at 37 °C for 10 min before transferring to ice ...
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bioRxiv - Cell Biology 2021Quote: ... kept for 2 h in DMEM and were labeled with 1µM Tet-Cy3 (Jena Bioscience, CLK-014-05) or Tet-BDP-FL (Jena Bioscience ...
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bioRxiv - Cell Biology 2019Quote: ... 12 μM unlabeled tubulin + 1 μM Alexa-568 tubulin + 1 μM biotin tubulin was polymerized in BRB80 (80 mM Pipes, 1 mM EGTA, 1 mM MgCl2) in the presence of 1 mM GMPCPP (Jena Bioscience, NU-405L) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... as in 74 from a mixture of unlabeled, rhodamine- and biotin-labeled tubulins (8:1:1, total tubulin concentration 5 μM) supplemented with 1 mM GMPCPP (Jena Bioscience, NU-405L). A flow chamber was prepared as for assays with taxol-stabilized microtubules ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM GTP (Roth, K056.4) or 1 mM GMP (Jena Biosciences, JBS-NU-1028), was added to the washed beads and incubated gently by rocking for 30 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: The samples were vitrified in liquid ethane on glow-discharged 200 copper mesh R1.2/1.3 Quantifoil holey carbon coated grids with 2 nm continuous carbon on top (Jena Bioscience) using a Leica EM GP plunger at 85 % humidity with 1.5 s blotting time ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM GMPCPP (Jena Biosciences) and 4 mM MgCl2 ...
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bioRxiv - Microbiology 2023Quote: ... cultures (with an OD600 of approximately 0.3) were pulse-labeled with 250 µM 4-azido-L-homoalanine (AHA, Jena Bioscience, Cat. No. CLK-AA005) and incubated for 15 min at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GMPCPP (Jena Bioscience, Jena, Germany). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GMPCPP (Jena Bioscience, Jena, Germany). The polymerized microtubules were centrifuged for 30 min at 18.000 x g in a Microfuge 18 Centrifuge (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... and 1 μL of 3-azido-7-hydroxycoumarin solution (1 mM in EtOH; Jena Bioscience) was added ...
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bioRxiv - Neuroscience 2024Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GTP (Jena Bioscience, Jena, Germany) for 30 minutes at 37 °C prior to centrifugation at 18.000 x g for 30 min in a Microfuge 18 Centrifuge (Beckman Coulter ...
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bioRxiv - Biochemistry 2020Quote: Guanosine nucleotides labeled with fluorescent N-methyl-anthranoyl (mant) groups O-linked with 2’ or 3’ of ribose (Jena Biosciences), were used for binding studies ...
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bioRxiv - Immunology 2021Quote: ... Azide-coupled Nbs were labeled by SPAAC (strain-promoted azide-alkyne cycloaddition) click chemistry reaction with 2-fold molar excess of DBCO-Cy5.5 (Jena Bioscience) for 2 h at 25°C ...
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bioRxiv - Microbiology 2020Quote: ... Genome-labeled AdV was produced by growing the virus in A549 cells in the presence of 2.5 μM EdC (5-ethynyl-2’-deoxycytidine, Jena Biosciences) as described 52 ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then incubated with a 2–fold excess of ε–ATP or ε–ADP (Jena Bioscience, Jena, Germany) for two hours on ice ...
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bioRxiv - Biophysics 2021Quote: ... 1 mM GMPCPP (Jena Bioscience, Germany), 1 mM MgCl2 and 4 mg mL-1 of porcine brain tubulin ...
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bioRxiv - Cell Biology 2021Quote: ... with 1 mM GMPCPP (Jena Bioscience) in PEM80 (80 mM Pipes ...
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bioRxiv - Biochemistry 2023Quote: ... and 1 mM GTP (Jena Bioscience). A 12.5 µM solution of fluorescently labeled tubulin in 1x assay buffer containing an oxygen scavenger system (freshly mixed 0.035 mg/ml catalase (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... Azide-coupled Nbs were then labelled by SPAAC (strain- promoted azide-alkyne cycloaddition) click chemistry reaction with 2-fold molar excess of Alexa-Fluor conjugated dibenzocyclooctyne (DBCO-AF647, Jena Bioscience) for 2 h at 25 °C ...
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bioRxiv - Molecular Biology 2022Quote: All samples were run in technical triplicates using the following PCR program (Table 2) on a qTOWER3/G cycler (Jena Biosciences).
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bioRxiv - Molecular Biology 2022Quote: ... Supernatants from the centrifugation of the disrupted cells were incubated with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) agarose (Jena Bioscience) on ice for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclear run-on (NRO) assays were performed with 2 biotin-11-NTPs (biotin-11-CTP and biotin-11-UTP, Jena Bioscience) as described in D ...
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bioRxiv - Microbiology 2021Quote: ... GMPPNP binding Irgb6 crystals diffracting to 1.6 Å resolution were obtained from sitting drops with a 9 mg/ml protein solution containing 2 mM GMPPNP (Jena Bioscience) and a reservoir solution consisting of 0.1 M Sodium citrate buffer pH 5.6 ...
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bioRxiv - Immunology 2023Quote: ... the SPAAC (strain-promoted azide-alkyne cycloaddition) click chemistry reaction was employed by incubating azide-coupled Nbs with 2-fold molar excess of DBCO-AF647 (Jena Bioscience) for 2 h at 25°C ...
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bioRxiv - Physiology 2023Quote: ... and cDNA was synthesised from 2 µg of RNA by reverse transcription (SCRIPT cDNA Synthesis Kit, Jena Bioscience GmBH, Jena, Germany). Gene expression assays for Il1b (Rn00580432_m1) ...
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bioRxiv - Cell Biology 2024Quote: ... were polymerized from 4 mg/ml tubulin for 2 h at 37°C in BRB80 supplemented with 1mM MgCl2 and 1mM GMPCPP (#NU-405, Jena Bioscience). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2019Quote: ... and incubated for 2 hours at 37°C in the absence or presence of N6-methyladenosine (Jena Biosciences, Cat no. NU-1101L) to generate ‘body-labeled’ m6A-RNAs ...