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Citations for Jena Bioscience :
151 - 200 of 223 citations for 5 Pyrimidinecarboxaldehyde 2 1 1 dimethylethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... MTs were prepared either with 0.75 µM mung tubulin or 30 µM goat brain tubulin in BRB-80 buffer with 1 mM GMPCPP (Jena Bioscience, Germany) and incubated at 37°C for 60 min ...
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bioRxiv - Microbiology 2020Quote: ... The assay was carried out at 37°C and the reaction was started by addition of 1 mM pppGpp (Jena Bioscience), and samples for the nucleotide measurement were taken after 15 min ...
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bioRxiv - Biochemistry 2020Quote: ... Additives as indicated were added during the primary antibody incubation step at a final concentration of 1 µM for adenosine (Jena Bioscience), AMP (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... purified naïve C57BL/6 B2 cells were preincubated with a mixture of NIP-OVA and HIV-1 p17/p24/gp120 fusion protein (Jena Biosciences) covalently bound to 1 μm beads (1:1 bead:Bcell ratio ...
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bioRxiv - Cell Biology 2020Quote: ... For synthesis of the 4sU-labeled spike-ins 1/10 of UTP in the transcription reactions was replaced with 4sUTP (Jena BioScience).
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bioRxiv - Molecular Biology 2019Quote: ... GMPCPP-stabilized seeds were made by two rounds of polymerization in 25 µM tubulin (40% dig-tubulin) supplemented with 1 mM GMPCPP (Jena Biosciences) as described (Volkov et al. ...
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bioRxiv - Physiology 2022Quote: ... glycoproteins were enriched using Concanavalin A and azido-modifed proteins were immobilized on magnetic DBCO-beads (Jena Bioscience #CLK-1037-1) via click chemistry ...
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bioRxiv - Microbiology 2023Quote: ... 500 ng of RNA was reverse-transcribed with gene specific primers (Supplementary information 1) using the SCRIPT cDNA Synthesis Kit (Jena Bioscience) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: On-gel mito-FUNCAT was performed as described previously.72,114 HeLa S3 and MPV17L2 KO #1 cells were cultured in 6-well plates in methionine-free medium with 50 µM HPG (Jena Bioscience) and 100 µg/ml anisomycin (Alomone Labs ...
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bioRxiv - Biochemistry 2023Quote: ... The chamber was pre-equilibrated with an oxygen scavenger cocktail 32 in 20 mM HEPES pH 7.4 with 150 mM NaCl and time-lapse images were acquired while flowing desired proteins in the same buffer in the absence or presence of 1 mM GTP (Jena Bioscience) and 1 mM MgCl2.
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bioRxiv - Microbiology 2024Quote: ... The following primary antibodies were diluted 1:400 in blocking buffer and incubated over night at 4°C: mouse anti-dsRNA J2 (RNT-SCI-10010200; Jena bioscience), mouse anti-DDX5 (67025 Proteintech ...
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bioRxiv - Biochemistry 2021Quote: ... RHEB was preincubated for 1 h with a 30-fold molar excess of GTPγS (Jena Bioscience NU-412-20, lot IT008-18). Next ...
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bioRxiv - Biochemistry 2023Quote: ... the sequence of corresponding constructs of proLEG were cloned into pLEXSY-sat2.1 vectors and transfected into LEXSY P10 host strain of Leishmania tarentolae cells of the LEXSYcon2.1 expression system (Jena Biosciences, Jena, Germany). The resulting constructs carried N-terminal His6-tags and an N-terminal signal sequence for secretion into the supernatant ...
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bioRxiv - Developmental Biology 2023Quote: ... After two additional washes the Tn5 was added (1:100) in CUT&Tag med buffer (20 mM HEPES [pH 7.5] (Jena Bioscience, #CSS-511), 300 mM NaCl (Sigma Aldrich ...
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bioRxiv - Synthetic Biology 2023Quote: ... we added all four nucleotides at a final concentration of 1 µM (Biotin-7-dATP, Jena Biosciences, and dC-Atto647N or dU-Atto488, Jena Biosciences with respective pair of unmodified nucleotides ...
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bioRxiv - Biochemistry 2020Quote: ... 3’dGTP and cytidine-5’-[(α,β)-methyleno]triphosphate (CMPCPP) were from Jena Bioscience (Jena, Germany); 2’dGTP ...
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bioRxiv - Plant Biology 2021Quote: ... The in vitro transcription reaction contained 0.25 mM 5-Azido-C3-UTP (Jena Bioscience, Jena, Germany). Purified RNA probes were Click-labelled with either Cy5.5-alkyne or Cy7.5-alkyne (Lumiprobe ...
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bioRxiv - Molecular Biology 2022Quote: ... USA (Catalog No. S3190) and 7-Methyl-guanosine-5’-monophosphate (m7GMP) was purchased from Jena Bioscience, Jena ...
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bioRxiv - Molecular Biology 2022Quote: ... 24% PEG 3350 and 1mM Anderson−Evans polyoxotungstate [TeW6O24]6− (TEW) (Jena Biosciences #X-TEW-5) to improve crystal diffraction ...
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bioRxiv - Cell Biology 2023Quote: ... Guanosine-5’-[(α,β)-methyleno] triphosphate (GMPCPP) was purchased from Jena Bioscience (NU-405S, Jena, Germany,). Glutathione Sepharose 4B beads (17075605 ...
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bioRxiv - Cell Biology 2023Quote: ... Guanosine-5’-[(α,β)-methyleno] triphosphate (GMPCPP) was purchased from Jena Bioscience (NU-405S, Jena, Gemany). Taxol was purchased from Cytoskeleton (TXD01 ...
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bioRxiv - Genomics 2023Quote: ... instead of UTP or N4-Acetyl-cytidine-5’-triphosphate (N4-Acetyl-CTP, Jena Bioscience, NU-988S) instead of CTP ...
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bioRxiv - Cell Biology 2019Quote: ... fluorescently labelled and non-labelled tubulin in a ratio 18:12:70 (total of 20 μM tubulin) with 1 mM GMPCPP (NU-405 Jena BioScience, Jena, Germany) at 37°C for 30 min ...
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bioRxiv - Genomics 2023Quote: ... 3 µL of 1 mM each dATP/ddCTP/ddGTP/ddTTP (dATP/ddBTP) (dATP, Thermo Fisher R0141; ddCTP/ddGTP/ddTTP, Jena Bioscience NU-1019S), and 7.5 U of Klenow fragment 3’→5’ exo-(NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... and GTP or GDPCP were assembled in 1×10 buffer with 10 mM βME by preincubation of eEF1A and GTP (PromegaTM Corp., Madison, WI) or GDPCP (Jena Bioscience, Jena, Germany) for 5 minutes at 37°C followed by addition of tRNAAla or Ala-tRNAAla for 1 minute at 37°C and were then kept on ice until use ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were injected with either 50 mM or 250 mM 5-ethynyl uridine (Jena Biosciences, Jena, Germany) using a PLI-100 Plus Pico-Injector (Harvard Apparatus ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and incubated with 2 ml of Ni-NTA Agarose (Jena Bioscience) pre-equilibrated with the lysis buffer for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: All nucleoside triphosphates (NTPs) and 2’-deoxy nucleoside triphosphates (dNTPs) were purchased from Jena Biosciences. Chemical and reagents used in synthesis were obtained from Merck ...
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bioRxiv - Biophysics 2020Quote: ... pH = 6.9) with 1mM MgCl2 and 1mM guanosine-5’-[(α,β)-methyleno] triphosphate (GMPCPP, Jena Bioscience, Jena, Germany) for 1.5 h at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells were incubated with pre-warmed fresh media with 400 µM 5-ethynyl uridine (5EU, Jena Biosciences) for 1 ...
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bioRxiv - Biochemistry 2022Quote: ... eluted C3-SC1TECs were pre-warmed at 37 °C for 2 minutes before 18.75 μl of C3-SC1TECs were mixed with 6.25 μl of pre-warmed Chase Mix G (2.5 μl 5 mM NTPs, 2.5 μl 100 μg/ml rifampicin, 1.25 μl of ppGpp (Jena Bioscience) in nuclease-free water at 20X final ppGpp concentration) ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... Doubly stabilized MTs were polymerized by incubating 5 µM tubulin with 0.5 mM GMPCPP (Jena BioScience, #NU-405L) for 20 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... All exchange experiments reported in this article were initiated by incubation of G-actin (1 µM) with N6-(6-Amino)hexyl-ATP-ATTO-488 (0.1 µM; Jena Bioscience, Germany, ref. NU-805-488) in G+ME buffer (5mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were labeled at the 3’ end using pCp-Cy5 (Cytidine-5’-phosphate-3’-(6-aminohexyl) phosphate) (Jena Biosciences). For each binding experiment ...
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bioRxiv - Genetics 2021Quote: ... the PCR labelling Cy-3 and Cy-5 kits (Jena Bioscience, ref# PP-301L-Cy3 and #APP-101-Cy5) according to the manufacturer’s instructions with M13 universal primers ...
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bioRxiv - Microbiology 2020Quote: ... For phasing the crystals were soaked overnight in 50 mM 5-amino-2,4,6-triiodoisophthalic acid (I3C)/LiOH (Magic Triangle, Jena Biosciences)42 in cryobuffer ...
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bioRxiv - Plant Biology 2023Quote: ... RNA probes were prepared by in vitro transcription in the presence of 5-azido-C3-UTP nucleotides (Jena Biosciences) with final linking to sulpho-cyanine cy5.5 or cy7.5 dye (Lumiprobe ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown on coverslips and transcription levels were measured by pulse labeling with 5’ethynyl uridine (EU, Jena Bioscience) in Ham’s F10 medium supplemented with 10% dialyzed FCS and 20 mM HEPES buffer (both Gibco) ...
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bioRxiv - Biochemistry 2021Quote: ... RNAs were fluorescently labeled with cyanine 5 at the 3’ end with the help of pCp-Cy5 (Jena Bioscience, 84179) and T4 RNA ligase I (NEB ...
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bioRxiv - Pathology 2023Quote: Nascent RNA was labeled by intraperitoneal injection of 88 mg/kg 5-ethynyl-uridine (EU) (Jena Biosciences, #CLK-N002-10) to P25 mice ...
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bioRxiv - Biophysics 2023Quote: ... we ran PCR reactions with added biotin-16-dUTP or 5-DBCO-(PEG)4-dUTP (Jena Biosciences GmbH, Jena, Germany), respectively ...
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bioRxiv - Microbiology 2023Quote: ... 20 μl of the prepared protein were mixed with 5 μl of 500-fold diluted JBS Thermofluor Dye (Jena Bioscience), prepared in the same buffer ...
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bioRxiv - Cell Biology 2021Quote: ... kept for 2 h in DMEM and were labeled with 1µM Tet-Cy3 (Jena Bioscience, CLK-014-05) or Tet-BDP-FL (Jena Bioscience ...
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bioRxiv - Plant Biology 2022Quote: For dsRNA labeling with the fluorochrome we followed the same dsRNA synthesis method described above but including fluorescein-X-(5-aminoallyl)-UTP (Jena Bioscience) in the reaction mix following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... short oligo DNAs for each target (see Supplementary Table 6) were pooled and labelled with 5-Propargylamino-ddUTP-Cy3 or -Cy5 (Jena Biosciences) using the Terminal Deoxynucleotidyl Transferase (Thermo Fisher)52 ...
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bioRxiv - Immunology 2024Quote: ... Immunostaining was performed in four steps to avoid unspecific binding of the secondary antibodies: i) incubation overnight at 4LJ with the mouse monoclonal IgG2a J2 antibody anti-dsRNA (Jena Bioscience, cat. RNT-SCI-10010500, dilution 1:200), ii ...
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bioRxiv - Microbiology 2023Quote: The samples were vitrified in liquid ethane on glow-discharged 200 copper mesh R1.2/1.3 Quantifoil holey carbon coated grids with 2 nm continuous carbon on top (Jena Bioscience) using a Leica EM GP plunger at 85 % humidity with 1.5 s blotting time ...
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bioRxiv - Molecular Biology 2021Quote: ... The 100% m6A modified full-length PAN transcript was synthesized using the MEGAscript T7 Transcription Kit following the manufacturer’s protocol but substituting adenosine-triphosphate with N6-methyladenosine-5’-triphosphate (Jena Bioscience NU-1101S). RNAs were purified using the MEGAclear Transcription Clean-Up Kit (ThermoFisher AM1908).
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bioRxiv - Biochemistry 2023Quote: Nucleotide binding was analyzed using the non-hydrolysable GTP analogue N- methylanthraniloyl guanosine-5’-[(β,γ)-imido]triphosphate (mant-GMPPNP, Jena Bioscience). Varying protein concentrations of non-farnesylated GBP1 ranging from 0.02 µM to 40 µM were prepared in a 96-well plate in 50 mM Tris-HCl pH 7.9 ...
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bioRxiv - Biochemistry 2023Quote: In vitro transcribed RNase E 5’ UTR (4.5 µM) was incubated with 33 µM of pCp Cy5 (Jena Bioscience #NU-1706-CY5) in a total reaction volume of 100 µL consisting of 50 units of T4 RNA ligase 1 (NEB #M0204S) ...