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Citations for Jena Bioscience :
151 - 200 of 269 citations for 5 Pyrimidinecarbonitrile 1 2 3 4 tetrahydro 4 oxo 6 phenyl 2 thioxo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... AMP-CPP (α,β-methyleneadenosine 5’-triphosphate lithium salt, Jena Bioscience #NU-421-25), AMP-PCP (β,γ-Methyleneadenosine 5′-triphosphate disodium salt ...
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bioRxiv - Plant Biology 2023Quote: ... DNA oligonucleotides used as probes were extended with 5-Azido-PEG4-dCTP (Jena Bioscience) and terminal transferase (NEB) ...
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bioRxiv - Immunology 2021Quote: ... XCL1-K(N3) and sdAb-K(N3) were subsequently reacted with DBCO-Cy5.5 (3 equiv.) (CLK-1046, Jena BioSciences) overnight at RT on an end-over-end shaker ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 % of UTP (resp. GTP) was substituted with either 5-ethynyl-UTP (5EU, Jena Bioscience), 5-bromo-UTP (5BrU ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 pmols of RNA were incubated with 150 pmoles of pCp-Cy-5 (Jena Biosciences), 20 units of T4-RNA ligase (NEB) ...
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bioRxiv - Plant Biology 2021Quote: ... The in vitro transcription reaction contained 0.25 mM 5-Azido-C3-UTP (Jena Bioscience, Jena, Germany). Purified RNA probes were Click-labelled with either Cy5.5-alkyne or Cy7.5-alkyne (Lumiprobe ...
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bioRxiv - Molecular Biology 2022Quote: ... USA (Catalog No. S3190) and 7-Methyl-guanosine-5’-monophosphate (m7GMP) was purchased from Jena Bioscience, Jena ...
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bioRxiv - Genomics 2023Quote: ... instead of UTP or N4-Acetyl-cytidine-5’-triphosphate (N4-Acetyl-CTP, Jena Bioscience, NU-988S) instead of CTP ...
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bioRxiv - Cell Biology 2023Quote: ... Guanosine-5’-[(α,β)-methyleno] triphosphate (GMPCPP) was purchased from Jena Bioscience (NU-405S, Jena, Germany,). Glutathione Sepharose 4B beads (17075605 ...
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bioRxiv - Cell Biology 2023Quote: ... Guanosine-5’-[(α,β)-methyleno] triphosphate (GMPCPP) was purchased from Jena Bioscience (NU-405S, Jena, Gemany). Taxol was purchased from Cytoskeleton (TXD01 ...
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bioRxiv - Molecular Biology 2023Quote: T7 promoter containing double stranded DNA (Supplementary Table 3) was used as a template for in vitro transcription with HighYield T7 mRNA Synthesis Kit (ac4CTP) (Jena Bioscience).
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were injected with either 50 mM or 250 mM 5-ethynyl uridine (Jena Biosciences, Jena, Germany) using a PLI-100 Plus Pico-Injector (Harvard Apparatus ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Dried lipid films were click reacted in a 40 μl reaction mix containing 0.45 mM fluorogenic dye 3-azido-7-hydroxycoumarin (Jena Bioscience, CLK-FA047), 1.4 mM Cu(I)tetra(acetonitrile ...
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bioRxiv - Evolutionary Biology 2024Quote: ... strains with MYO3 or MYO5 locus fused at its 3’ to the DHFR F[1,2] with a nourseothricin-resistance marker (NAT 100 μg/ml, Jena Bioscience GmbH, Germany). To insert the variant libraries instead of the wild-type SH3 domains ...
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bioRxiv - Biophysics 2020Quote: ... pH = 6.9) with 1mM MgCl2 and 1mM guanosine-5’-[(α,β)-methyleno] triphosphate (GMPCPP, Jena Bioscience, Jena, Germany) for 1.5 h at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells were incubated with pre-warmed fresh media with 400 µM 5-ethynyl uridine (5EU, Jena Biosciences) for 1 ...
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bioRxiv - Biochemistry 2022Quote: ... eluted C3-SC1TECs were pre-warmed at 37 °C for 2 minutes before 18.75 μl of C3-SC1TECs were mixed with 6.25 μl of pre-warmed Chase Mix G (2.5 μl 5 mM NTPs, 2.5 μl 100 μg/ml rifampicin, 1.25 μl of ppGpp (Jena Bioscience) in nuclease-free water at 20X final ppGpp concentration) ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... Doubly stabilized MTs were polymerized by incubating 5 µM tubulin with 0.5 mM GMPCPP (Jena BioScience, #NU-405L) for 20 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... For phasing the crystals were soaked overnight in 50 mM 5-amino-2,4,6-triiodoisophthalic acid (I3C)/LiOH (Magic Triangle, Jena Biosciences)42 in cryobuffer ...
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bioRxiv - Plant Biology 2023Quote: ... RNA probes were prepared by in vitro transcription in the presence of 5-azido-C3-UTP nucleotides (Jena Biosciences) with final linking to sulpho-cyanine cy5.5 or cy7.5 dye (Lumiprobe ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown on coverslips and transcription levels were measured by pulse labeling with 5’ethynyl uridine (EU, Jena Bioscience) in Ham’s F10 medium supplemented with 10% dialyzed FCS and 20 mM HEPES buffer (both Gibco) ...
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bioRxiv - Pathology 2023Quote: Nascent RNA was labeled by intraperitoneal injection of 88 mg/kg 5-ethynyl-uridine (EU) (Jena Biosciences, #CLK-N002-10) to P25 mice ...
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bioRxiv - Microbiology 2023Quote: ... 20 μl of the prepared protein were mixed with 5 μl of 500-fold diluted JBS Thermofluor Dye (Jena Bioscience), prepared in the same buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 0.9 mL of DNase solution (15 mM MgSO4, 1 mM DTT and 1 mM PMSF, 1 x Turbo DNase (Jena biosciences)) was added and the samples were allowed to shake at 37 °C for 10 min before transferring to ice ...
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bioRxiv - Plant Biology 2022Quote: For dsRNA labeling with the fluorochrome we followed the same dsRNA synthesis method described above but including fluorescein-X-(5-aminoallyl)-UTP (Jena Bioscience) in the reaction mix following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 12 μM unlabeled tubulin + 1 μM Alexa-568 tubulin + 1 μM biotin tubulin was polymerized in BRB80 (80 mM Pipes, 1 mM EGTA, 1 mM MgCl2) in the presence of 1 mM GMPCPP (Jena Bioscience, NU-405L) for 1 h at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM GTP (Roth, K056.4) or 1 mM GMP (Jena Biosciences, JBS-NU-1028), was added to the washed beads and incubated gently by rocking for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM GMPCPP (Jena Biosciences) and 4 mM MgCl2 ...
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bioRxiv - Molecular Biology 2021Quote: ... The 100% m6A modified full-length PAN transcript was synthesized using the MEGAscript T7 Transcription Kit following the manufacturer’s protocol but substituting adenosine-triphosphate with N6-methyladenosine-5’-triphosphate (Jena Bioscience NU-1101S). RNAs were purified using the MEGAclear Transcription Clean-Up Kit (ThermoFisher AM1908).
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bioRxiv - Biochemistry 2023Quote: Nucleotide binding was analyzed using the non-hydrolysable GTP analogue N- methylanthraniloyl guanosine-5’-[(β,γ)-imido]triphosphate (mant-GMPPNP, Jena Bioscience). Varying protein concentrations of non-farnesylated GBP1 ranging from 0.02 µM to 40 µM were prepared in a 96-well plate in 50 mM Tris-HCl pH 7.9 ...
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bioRxiv - Biochemistry 2023Quote: In vitro transcribed RNase E 5’ UTR (4.5 µM) was incubated with 33 µM of pCp Cy5 (Jena Bioscience #NU-1706-CY5) in a total reaction volume of 100 µL consisting of 50 units of T4 RNA ligase 1 (NEB #M0204S) ...
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bioRxiv - Cell Biology 2020Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GMPCPP (Jena Bioscience, Jena, Germany). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GMPCPP (Jena Bioscience, Jena, Germany). The polymerized microtubules were centrifuged for 30 min at 18.000 x g in a Microfuge 18 Centrifuge (Beckman Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GTP (Jena Bioscience, Jena, Germany) for 30 minutes at 37 °C prior to centrifugation at 18.000 x g for 30 min in a Microfuge 18 Centrifuge (Beckman Coulter ...
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bioRxiv - Biophysics 2020Quote: ... as a function of load in the presence of the non-hydrolyzable ATP analog adenosine-5’-[(β, γ)-imido] triphosphate (AMPPNP, Jena Bioscience). With AMPPNP ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and 40 cycles of PCR with either the sense or anti-sense primer in a Taq polymerase PCR mixture to which 0.067 mM of Digoxigenin-5-aminoallyl-dUTP (Jena Bioscience GmbH, Jena, Germany) had been added and in which the concentration of dTTP had been lowered to 0.133 mM ...
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bioRxiv - Cell Biology 2021Quote: ... Biotin and Alexa-Fluor-633-labeled porcine tubulin were generated in the laboratory as described (Al-Bassam, 2014) and were polymerized using the non-hydrolysable GTP analog guanosine-5’-[(α,β)-methyleno] triphosphate (termed GMPCPP; Jena Biosciences) or using the MT stabilizing drug ...
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bioRxiv - Biophysics 2019Quote: ... Biotin and Alexa-Fluor-633-labeled porcine tubulin were generated in the laboratory as described (Al-Bassam, 2014) and were polymerized using the non-hydrolyzable GTP analog guanosine-5’-[(α,β)-methyleno] triphosphate (termed GMPCPP; Jena Biosciences) or using the MT stabilizing drug ...
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bioRxiv - Plant Biology 2022Quote: ... The adenosine-5’-[(β,γ)-methyleno]triphosphate (APPCP) complex was prepared by soaking crystals in crystallization buffer supplemented with 10 mM APPCP (Jena Biosciences), 10 mM uridine and 10 mM MgCl2 for 3 min ...
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bioRxiv - Pathology 2022Quote: ... extracts were treated with 6μL/100μL of extract of a premix solution containing: 60μM of TAMRA-biotin-azide (Jena Bioscience, catalog number CLK-1048-5), 1mM CuSO4 in water (freshly prepared) ...
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bioRxiv - Biophysics 2021Quote: ... 1 mM GMPCPP (Jena Bioscience, Germany), 1 mM MgCl2 and 4 mg mL-1 of porcine brain tubulin ...
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bioRxiv - Cell Biology 2021Quote: ... with 1 mM GMPCPP (Jena Bioscience) in PEM80 (80 mM Pipes ...
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bioRxiv - Biochemistry 2023Quote: ... and 1 mM GTP (Jena Bioscience). A 12.5 µM solution of fluorescently labeled tubulin in 1x assay buffer containing an oxygen scavenger system (freshly mixed 0.035 mg/ml catalase (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... The solubilised proteins in the supernatant were used for the click reaction in a final volume of 120 μl (0.2% w/v final SDS concentration) with the following components added in order to the indicated final concentrations: 20 μM picolyl-biotin-azide (Jena Bioscience CLK-1167-5), 1.2 mM BTTAA (Jena Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... the thawed cryo-sections were subjected to a CuAAC reaction (as described above) using 5 mM biotin-azide in DMSO (> 95% purity, Jena Bioscience GmbH, Jena, Germany) instead of Azide-Fluor 488 ...
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bioRxiv - Biochemistry 2020Quote: ... AF488-Azide (#CLK-1275-1, Jena Bioscience) was used to test success of bead – DBCO coupling ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM GMPPCP (NU-405S, Jena Bioscience) or 1 mM BeF4 (prepared by mixing 50 mM BeSO4 and NaF 100 mM to make 10 mM BeF3) ...
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bioRxiv - Microbiology 2021Quote: ... Labeling was achieved by using a 1:1 molar ratio of dCTP:Biotin-11-dCTP (NU-809-BIOX, Jena Bioscience) in the dNTPs used for the PCR (GoTaq DNA polymerase ...
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bioRxiv - Cell Biology 2022Quote: ... was incubated with 1 mM GMPCPP (Jena Biosciences) at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and 50 µg mL-1 nourseothricin (Jena Bioscience).