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Citations for Jena Bioscience :
51 - 86 of 86 citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... pH = 6.9) with 1mM MgCl2 and 1mM guanosine-5’-[(α,β)-methyleno] triphosphate (GMPCPP, Jena Bioscience, Jena, Germany) for 1.5 h at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells were incubated with pre-warmed fresh media with 400 µM 5-ethynyl uridine (5EU, Jena Biosciences) for 1 ...
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bioRxiv - Biochemistry 2022Quote: ... eluted C3-SC1TECs were pre-warmed at 37 °C for 2 minutes before 18.75 μl of C3-SC1TECs were mixed with 6.25 μl of pre-warmed Chase Mix G (2.5 μl 5 mM NTPs, 2.5 μl 100 μg/ml rifampicin, 1.25 μl of ppGpp (Jena Bioscience) in nuclease-free water at 20X final ppGpp concentration) ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... Doubly stabilized MTs were polymerized by incubating 5 µM tubulin with 0.5 mM GMPCPP (Jena BioScience, #NU-405L) for 20 min at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... then 5×106 cells were plated on ESAW agar plates containing 450 μg mL-1 nourseothricin (Jena Bioscience), and incubated in constant light at 18 °C.
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were labeled at the 3’ end using pCp-Cy5 (Cytidine-5’-phosphate-3’-(6-aminohexyl) phosphate) (Jena Biosciences). For each binding experiment ...
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bioRxiv - Genetics 2021Quote: ... the PCR labelling Cy-3 and Cy-5 kits (Jena Bioscience, ref# PP-301L-Cy3 and #APP-101-Cy5) according to the manufacturer’s instructions with M13 universal primers ...
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bioRxiv - Microbiology 2020Quote: ... For phasing the crystals were soaked overnight in 50 mM 5-amino-2,4,6-triiodoisophthalic acid (I3C)/LiOH (Magic Triangle, Jena Biosciences)42 in cryobuffer ...
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bioRxiv - Microbiology 2023Quote: ... and pellets were resuspended using 1/5 or 1/10 dilutions of detergents stocks from the JBScreen (Jena Bioscience) or Detergent Screen (Hampton) ...
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bioRxiv - Plant Biology 2023Quote: ... RNA probes were prepared by in vitro transcription in the presence of 5-azido-C3-UTP nucleotides (Jena Biosciences) with final linking to sulpho-cyanine cy5.5 or cy7.5 dye (Lumiprobe ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown on coverslips and transcription levels were measured by pulse labeling with 5’ethynyl uridine (EU, Jena Bioscience) in Ham’s F10 medium supplemented with 10% dialyzed FCS and 20 mM HEPES buffer (both Gibco) ...
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bioRxiv - Biophysics 2020Quote: ... 5’-TAGTCCAGAACGAGACCGCAACAGCACAACCCAAACTG-3’ and 5’-AATCTGCTGCAATGCCACAG-3’ (underline section indicates overhang) with a 10:1 ratio of dTTP:digoxigenin-11-dUTP (Jena Bioscience). The labelled fragments were purified using a PCR purification kit (QIAquick ...
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bioRxiv - Biochemistry 2021Quote: ... cells were labelled for 20 minutes with EU (5-ethynyl uridine, Jena Bioscience CLK-N002, final concentration at 1 mM). Harvested cells were firstly labeled with Zombie AquaTM (BioLegend ...
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bioRxiv - Biochemistry 2021Quote: ... RNAs were fluorescently labeled with cyanine 5 at the 3’ end with the help of pCp-Cy5 (Jena Bioscience, 84179) and T4 RNA ligase I (NEB ...
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bioRxiv - Microbiology 2020Quote: ... Genome-labeled AdV was produced by growing the virus in A549 cells in the presence of 2.5 μM EdC (5-ethynyl-2’-deoxycytidine, Jena Biosciences) as described 52 ...
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bioRxiv - Pathology 2023Quote: Nascent RNA was labeled by intraperitoneal injection of 88 mg/kg 5-ethynyl-uridine (EU) (Jena Biosciences, #CLK-N002-10) to P25 mice ...
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bioRxiv - Biophysics 2023Quote: ... we ran PCR reactions with added biotin-16-dUTP or 5-DBCO-(PEG)4-dUTP (Jena Biosciences GmbH, Jena, Germany), respectively ...
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bioRxiv - Microbiology 2023Quote: ... 20 μl of the prepared protein were mixed with 5 μl of 500-fold diluted JBS Thermofluor Dye (Jena Bioscience), prepared in the same buffer ...
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bioRxiv - Plant Biology 2022Quote: For dsRNA labeling with the fluorochrome we followed the same dsRNA synthesis method described above but including fluorescein-X-(5-aminoallyl)-UTP (Jena Bioscience) in the reaction mix following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... short oligo DNAs for each target (see Supplementary Table 6) were pooled and labelled with 5-Propargylamino-ddUTP-Cy3 or -Cy5 (Jena Biosciences) using the Terminal Deoxynucleotidyl Transferase (Thermo Fisher)52 ...
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bioRxiv - Biophysics 2021Quote: ... GMPCPP seeds were prepared by polymerizing a 1:4 molar ratio of TAMRA labelled:unlabelled tubulin in the presence of guanosine-5’-[(α, β)-methyleno]triphosphate (GMPCPP, Jena Biosciences) in two cycles ...
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bioRxiv - Molecular Biology 2021Quote: ... The 100% m6A modified full-length PAN transcript was synthesized using the MEGAscript T7 Transcription Kit following the manufacturer’s protocol but substituting adenosine-triphosphate with N6-methyladenosine-5’-triphosphate (Jena Bioscience NU-1101S). RNAs were purified using the MEGAclear Transcription Clean-Up Kit (ThermoFisher AM1908).
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bioRxiv - Microbiology 2020Quote: ... The cells were then washed again 3x in 1xPBS and then incubated with 5 µM DBCO-488 (Jena Bioscience, CLK-1278-1) at 37 °C for 30 min or 5 µM Click-IT Alexa Fluor® 488 DIBO alkyne dye (ThermoScientific ...
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bioRxiv - Biochemistry 2022Quote: Guanine nucleotide exchange activity was measured by following changes in the fluorescence of the GDP derivative mant-dGDP [2’-Deoxy-3’-O-(N-Methyl-anthraniloyl) guanosine-5’-diphosphate sodium salt (Jena Bioscience GmbH)] (Margarit et al. ...
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bioRxiv - Biochemistry 2023Quote: Nucleotide binding was analyzed using the non-hydrolysable GTP analogue N- methylanthraniloyl guanosine-5’-[(β,γ)-imido]triphosphate (mant-GMPPNP, Jena Bioscience). Varying protein concentrations of non-farnesylated GBP1 ranging from 0.02 µM to 40 µM were prepared in a 96-well plate in 50 mM Tris-HCl pH 7.9 ...
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bioRxiv - Biochemistry 2023Quote: In vitro transcribed RNase E 5’ UTR (4.5 µM) was incubated with 33 µM of pCp Cy5 (Jena Bioscience #NU-1706-CY5) in a total reaction volume of 100 µL consisting of 50 units of T4 RNA ligase 1 (NEB #M0204S) ...
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bioRxiv - Biophysics 2020Quote: ... as a function of load in the presence of the non-hydrolyzable ATP analog adenosine-5’-[(β, γ)-imido] triphosphate (AMPPNP, Jena Bioscience). With AMPPNP ...
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bioRxiv - Cell Biology 2020Quote: ... from a mixture of unlabeled and HiLyte647-labeled tubulin (5:1, total tubulin concentration 100 μM) and 1 mM GMPCPP (Jena Bioscience, Jena, Germany) incubated at 37°C for 20 min ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and 40 cycles of PCR with either the sense or anti-sense primer in a Taq polymerase PCR mixture to which 0.067 mM of Digoxigenin-5-aminoallyl-dUTP (Jena Bioscience GmbH, Jena, Germany) had been added and in which the concentration of dTTP had been lowered to 0.133 mM ...
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bioRxiv - Cell Biology 2021Quote: ... Biotin and Alexa-Fluor-633-labeled porcine tubulin were generated in the laboratory as described (Al-Bassam, 2014) and were polymerized using the non-hydrolysable GTP analog guanosine-5’-[(α,β)-methyleno] triphosphate (termed GMPCPP; Jena Biosciences) or using the MT stabilizing drug ...
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bioRxiv - Biophysics 2019Quote: ... Biotin and Alexa-Fluor-633-labeled porcine tubulin were generated in the laboratory as described (Al-Bassam, 2014) and were polymerized using the non-hydrolyzable GTP analog guanosine-5’-[(α,β)-methyleno] triphosphate (termed GMPCPP; Jena Biosciences) or using the MT stabilizing drug ...
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bioRxiv - Plant Biology 2022Quote: ... The adenosine-5’-[(β,γ)-methyleno]triphosphate (APPCP) complex was prepared by soaking crystals in crystallization buffer supplemented with 10 mM APPCP (Jena Biosciences), 10 mM uridine and 10 mM MgCl2 for 3 min ...
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bioRxiv - Pathology 2022Quote: ... extracts were treated with 6μL/100μL of extract of a premix solution containing: 60μM of TAMRA-biotin-azide (Jena Bioscience, catalog number CLK-1048-5), 1mM CuSO4 in water (freshly prepared) ...
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bioRxiv - Cell Biology 2023Quote: ... as in 74 from a mixture of unlabeled, rhodamine- and biotin-labeled tubulins (8:1:1, total tubulin concentration 5 μM) supplemented with 1 mM GMPCPP (Jena Bioscience, NU-405L). A flow chamber was prepared as for assays with taxol-stabilized microtubules ...
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bioRxiv - Cell Biology 2020Quote: ... The solubilised proteins in the supernatant were used for the click reaction in a final volume of 120 μl (0.2% w/v final SDS concentration) with the following components added in order to the indicated final concentrations: 20 μM picolyl-biotin-azide (Jena Bioscience CLK-1167-5), 1.2 mM BTTAA (Jena Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... the thawed cryo-sections were subjected to a CuAAC reaction (as described above) using 5 mM biotin-azide in DMSO (> 95% purity, Jena Bioscience GmbH, Jena, Germany) instead of Azide-Fluor 488 ...