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Citations for Jena Bioscience :
51 - 88 of 88 citations for Recombinant Human CD274 Biotin His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and 32μg of protein was clicked to 31μM biotin-alkyne (Jena biosciences, #CLK-TA105) mixed in PBS pH 7.8-PI (Calbiochem ...
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bioRxiv - Microbiology 2023Quote: ... This buffer contained 50 µM acetylene-PEG4-Biotin (Jena Bioscience, Cat. No. CLK-TA105), 1 mM TCEP (Tris(2-carboxyethyl)phosphine hydrochloride Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... supernatants were supplemented with 50 µM trifunctional linker TAMRA-azide-PEG3-biotin (TFL, Jena Bioscience), and click chemistry performed as described ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with 0.5 nmol picolyl-azide PEG4-biotin in 7.5 ml PBS (Jena Bioscience) per dish ...
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bioRxiv - Genomics 2023Quote: ... Probe for FISH experiment was prepared by PCR labeling with biotin-16-dUTP (Jena BioScience) using M ...
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bioRxiv - Neuroscience 2023Quote: ... EPEA-displaying VLPs were first biotinylated using biotin-PEG4-NHS ester (Jena Bioscience, CLK-B103), after which it was immobilised on a 4PCP-STA streptavidin chip to a final density of 58 pg/mm2 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were subjected to click-chemistry reaction with 20mM of Cy5-or Biotin-azide (Jena Bioscience) for 30min ...
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bioRxiv - Biophysics 2019Quote: ... MA USA.) and either biotin-16-dUTP or digoxigenin-11-dUTP (Jena Biosciences GmbH, Jena, Germany) was added to the PCR reactions to a final concentration of 40 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... each pellet was resuspended in 5mL of click reaction mix (10µM biotin azide, Jena Bioscience CLK-047), 10mM sodium ascorbate ...
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bioRxiv - Cancer Biology 2022Quote: ... the beads were treated with terminal deoxynucleotidyl transferase (EP0162) and biotinylated nucleotides (Biotin-11-dCTP, Jena Bioscience). The beads were then washed with IP buffer (50mM Tris–HCl pH 7.5 ...
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bioRxiv - Synthetic Biology 2023Quote: ... we added all four nucleotides at a final concentration of 1 µM (Biotin-7-dATP, Jena Biosciences, and dC-Atto647N or dU-Atto488 ...
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bioRxiv - Cell Biology 2024Quote: ... 20 OD254 units of polysomes were incubated with 100 pmol of biotin-dc-puromycin (Jena Bioscience, Germany) per OD254 unit at 37°C for 15 min ...
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bioRxiv - Immunology 2023Quote: ... 1 µg/ml of recombinant YF17D-DIII or TBEV-DIII (Jena Bioscience, PR-1450-S) was used ...
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bioRxiv - Neuroscience 2021Quote: ... or 90 min in ∼ 30 µL Tyrode’s with 10 µM biotin-AMP (Jena Bioscience; NU-894-BIO-S) and with or without 0.3 µM recombinant BirA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 12 O.D.254 U of polysomes were incubated with 100 pmol of biotin-dc-puromycin (Jena Bioscience, Germany) per O.D.254 unit at 37°C for 15 min ...
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bioRxiv - Microbiology 2020Quote: Purified recombinant BepA was used with proprietary crystal screens (supplied by Molecular Dimensions and Jena Bioscience) in sitting drop crystallization experiments using 2 μl of protein solution and 2 μl of crystallization mother liquor at 18°C ...
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bioRxiv - Microbiology 2019Quote: Purified recombinant BepA was used with proprietary crystal screens (supplied by Molecular Dimensions and Jena Bioscience) in sitting drop crystallization experiments using 2 µl of protein solution and 2 µl of crystallization mother liquor at 18°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were afterwards subjected to an upscaled click chemistry reaction with biotin-picolyl-azide (Jena Bioscience CLK-1167) for one hour at room temperature and washed twice with 10% ethanol in PBS afterwards ...
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bioRxiv - Neuroscience 2022Quote: ... 10µl of the beads / ligase mix were transferred to a new tube and 1µl of pCp-Biotin (Jena Bioscience) were added to validate IP of the RNA-protein-complexes by western blot ...
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bioRxiv - Genomics 2022Quote: ... human 22 with Atto-594-dUTPs and human 15 was labelled with Atto-425-dUTPs and Atto-594-dUTPs (Jena Bioscience). Hybridization was carried out overnight at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant DFCP1 was loaded with nucleotides by incubation with either 400 µM ADP or ATPγS (Jena Bioscience) in exchange buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... Click-it chemistry was performed using the Click-it reaction cocktail containing Azide-PEG3-Biotin Conjugate (20 μM, Jena Bioscience), 1× Click-it reaction buffer (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... we ran PCR reactions with added biotin-16-dUTP or 5-DBCO-(PEG)4-dUTP (Jena Biosciences GmbH, Jena, Germany), respectively ...
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bioRxiv - Genomics 2024Quote: ... and fragment ends filled-in and biotinylated by adding 27.5 µl of a cocktail containing 15 µL 1 mM biotin-14-dUTP (Jena BioScience), dCTP ...
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bioRxiv - Biophysics 2023Quote: ... The final gene construct was completed from the 3’-terminus by KpnI restriction site followed by 9×His-tag nucleotide sequence and inserted into the integrative inducible expression vector pLEXSY_I-blecherry3 (Jena Bioscience, Germany) via the BglII(5’ ...
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bioRxiv - Cancer Biology 2019Quote: ... We added 52 μL of incorporation master mix: 37.5 μL of 0.4 mM biotin–dATP (Jena Biosciences, NU-835-BIO14-S); 1.5 μL of a dCTP ...
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bioRxiv - Evolutionary Biology 2019Quote: The whole bonobo Y chromosome painting probe (WBY) was prepared from flow-sorted bonobo Y chromosomes and labeled with biotin-16-dUTP (Jena BioScience) using DOP-PCR according to (Yang et al ...
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bioRxiv - Genomics 2019Quote: ... a modified RNA linker ligation was performed with standard eCLIP ligation conditions (buffer and High Concentration T4 RNA Ligase) but with 500 pmol pCp-Biotin (Jena Bioscience) in place of the RNA adapter ...
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bioRxiv - Molecular Biology 2021Quote: The in vitro RNA-primed DNA replication assay was carried out using M13mp18 ssDNA as the template and in the presence of Biotin-11-dCTP (Jena Bioscience). Reactions (20 µl ...
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bioRxiv - Synthetic Biology 2023Quote: ... we added all four nucleotides at a final concentration of 1 µM (Biotin-7-dATP, Jena Biosciences, and dC-Atto647N or dU-Atto488, Jena Biosciences with respective pair of unmodified nucleotides ...
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bioRxiv - Biophysics 2021Quote: ... we labeled the vimentin monomers with the fluorescent dye ATTO647N (AD 647N-41, AttoTech, Siegen, Germany) and with biotin via malemeide (B1267-25MG, Jena Bioscience, Jena, Germany) as described in Refs ...
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bioRxiv - Pathology 2022Quote: ... extracts were treated with 6μL/100μL of extract of a premix solution containing: 60μM of TAMRA-biotin-azide (Jena Bioscience, catalog number CLK-1048-5), 1mM CuSO4 in water (freshly prepared) ...
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bioRxiv - Microbiology 2023Quote: ... Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) was performed by transferring 120 µL reagent-mixture containing 167 µM Biotin-Azide (Jena Bioscience, CLK-AZ104P4), 1.67 mM TBTA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... as in 74 from a mixture of unlabeled, rhodamine- and biotin-labeled tubulins (8:1:1, total tubulin concentration 5 μM) supplemented with 1 mM GMPCPP (Jena Bioscience, NU-405L). A flow chamber was prepared as for assays with taxol-stabilized microtubules ...
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bioRxiv - Cell Biology 2020Quote: ... The solubilised proteins in the supernatant were used for the click reaction in a final volume of 120 μl (0.2% w/v final SDS concentration) with the following components added in order to the indicated final concentrations: 20 μM picolyl-biotin-azide (Jena Bioscience CLK-1167-5), 1.2 mM BTTAA (Jena Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... the thawed cryo-sections were subjected to a CuAAC reaction (as described above) using 5 mM biotin-azide in DMSO (> 95% purity, Jena Bioscience GmbH, Jena, Germany) instead of Azide-Fluor 488 ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant full-length BGLF4 (EBV strain B95-8) was produced and purified from a commercially available Leishmania expression system (Jena Bioscience, Germany ...
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bioRxiv - Microbiology 2020Quote: ... and expression of the host Leishmania tarentolae strain T7-TR were performed according to the Jena Bioscience protocol for inducible expression of recombinant proteins secreted to medium (LEXSinduce Expression kit, Jena Bioscience, Germany). Expression of proKLK13 and proKLK14 was induced with 15 µg/ml of tetracycline (BioShop ...