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Citations for Jena Bioscience :
51 - 100 of 201 citations for Mouse IgG2b Isotype Control Antibody Biotin A 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Mouse anti-dsRNA J2 (1:100, RNT-SCI-10010200; Jena Bioscience), sheep anti-SARS-CoV-2 nsp3 (1:200 ...
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bioRxiv - Biophysics 2022Quote: ... The 4 bp overhangs formed were filled in with biotin-11-dGTP (Jena Bioscience), biotin-16-dUTP (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... This buffer contained 50 µM acetylene-PEG4-Biotin (Jena Bioscience, Cat. No. CLK-TA105), 1 mM TCEP (Tris(2-carboxyethyl)phosphine hydrochloride Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and 32μg of protein was clicked to 31μM biotin-alkyne (Jena biosciences, #CLK-TA105) mixed in PBS pH 7.8-PI (Calbiochem ...
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bioRxiv - Cell Biology 2021Quote: ... supernatants were supplemented with 50 µM trifunctional linker TAMRA-azide-PEG3-biotin (TFL, Jena Bioscience), and click chemistry performed as described ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with 0.5 nmol picolyl-azide PEG4-biotin in 7.5 ml PBS (Jena Bioscience) per dish ...
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bioRxiv - Genomics 2023Quote: ... Probe for FISH experiment was prepared by PCR labeling with biotin-16-dUTP (Jena BioScience) using M ...
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bioRxiv - Neuroscience 2023Quote: ... EPEA-displaying VLPs were first biotinylated using biotin-PEG4-NHS ester (Jena Bioscience, CLK-B103), after which it was immobilised on a 4PCP-STA streptavidin chip to a final density of 58 pg/mm2 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were subjected to click-chemistry reaction with 20mM of Cy5-or Biotin-azide (Jena Bioscience) for 30min ...
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bioRxiv - Biophysics 2019Quote: ... MA USA.) and either biotin-16-dUTP or digoxigenin-11-dUTP (Jena Biosciences GmbH, Jena, Germany) was added to the PCR reactions to a final concentration of 40 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... each pellet was resuspended in 5mL of click reaction mix (10µM biotin azide, Jena Bioscience CLK-047), 10mM sodium ascorbate ...
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bioRxiv - Cancer Biology 2022Quote: ... the beads were treated with terminal deoxynucleotidyl transferase (EP0162) and biotinylated nucleotides (Biotin-11-dCTP, Jena Bioscience). The beads were then washed with IP buffer (50mM Tris–HCl pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... 20 OD254 units of polysomes were incubated with 100 pmol of biotin-dc-puromycin (Jena Bioscience, Germany) per OD254 unit at 37°C for 15 min ...
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bioRxiv - Biophysics 2019Quote: ... Control reactions for azide-bearing proteins were performed using TAMRA-DBCO (Jena Bioscience). Reaction mixtures were incubated for varying durations (0.5 hours to overnight ...
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bioRxiv - Neuroscience 2021Quote: ... or 90 min in ∼ 30 µL Tyrode’s with 10 µM biotin-AMP (Jena Bioscience; NU-894-BIO-S) and with or without 0.3 µM recombinant BirA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 12 O.D.254 U of polysomes were incubated with 100 pmol of biotin-dc-puromycin (Jena Bioscience, Germany) per O.D.254 unit at 37°C for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were afterwards subjected to an upscaled click chemistry reaction with biotin-picolyl-azide (Jena Bioscience CLK-1167) for one hour at room temperature and washed twice with 10% ethanol in PBS afterwards ...
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bioRxiv - Neuroscience 2022Quote: ... 10µl of the beads / ligase mix were transferred to a new tube and 1µl of pCp-Biotin (Jena Bioscience) were added to validate IP of the RNA-protein-complexes by western blot ...
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bioRxiv - Microbiology 2023Quote: ... lysate was added to 30 µl m7G-functionalized (or control) agarose resin (Jena Bioscience) and incubated at 4°C for 4 h ...
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bioRxiv - Cell Biology 2021Quote: ... Click-it chemistry was performed using the Click-it reaction cocktail containing Azide-PEG3-Biotin Conjugate (20 μM, Jena Bioscience), 1× Click-it reaction buffer (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... we ran PCR reactions with added biotin-16-dUTP or 5-DBCO-(PEG)4-dUTP (Jena Biosciences GmbH, Jena, Germany), respectively ...
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bioRxiv - Cancer Biology 2019Quote: ... We added 52 μL of incorporation master mix: 37.5 μL of 0.4 mM biotin–dATP (Jena Biosciences, NU-835-BIO14-S); 1.5 μL of a dCTP ...
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bioRxiv - Evolutionary Biology 2019Quote: The whole bonobo Y chromosome painting probe (WBY) was prepared from flow-sorted bonobo Y chromosomes and labeled with biotin-16-dUTP (Jena BioScience) using DOP-PCR according to (Yang et al ...
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bioRxiv - Genomics 2019Quote: ... a modified RNA linker ligation was performed with standard eCLIP ligation conditions (buffer and High Concentration T4 RNA Ligase) but with 500 pmol pCp-Biotin (Jena Bioscience) in place of the RNA adapter ...
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bioRxiv - Molecular Biology 2021Quote: The in vitro RNA-primed DNA replication assay was carried out using M13mp18 ssDNA as the template and in the presence of Biotin-11-dCTP (Jena Bioscience). Reactions (20 µl ...
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bioRxiv - Biophysics 2021Quote: ... we labeled the vimentin monomers with the fluorescent dye ATTO647N (AD 647N-41, AttoTech, Siegen, Germany) and with biotin via malemeide (B1267-25MG, Jena Bioscience, Jena, Germany) as described in Refs ...
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bioRxiv - Pathology 2022Quote: ... extracts were treated with 6μL/100μL of extract of a premix solution containing: 60μM of TAMRA-biotin-azide (Jena Bioscience, catalog number CLK-1048-5), 1mM CuSO4 in water (freshly prepared) ...
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bioRxiv - Microbiology 2023Quote: ... Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) was performed by transferring 120 µL reagent-mixture containing 167 µM Biotin-Azide (Jena Bioscience, CLK-AZ104P4), 1.67 mM TBTA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... The solubilised proteins in the supernatant were used for the click reaction in a final volume of 120 μl (0.2% w/v final SDS concentration) with the following components added in order to the indicated final concentrations: 20 μM picolyl-biotin-azide (Jena Bioscience CLK-1167-5), 1.2 mM BTTAA (Jena Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... the thawed cryo-sections were subjected to a CuAAC reaction (as described above) using 5 mM biotin-azide in DMSO (> 95% purity, Jena Bioscience GmbH, Jena, Germany) instead of Azide-Fluor 488 ...
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bioRxiv - Microbiology 2024Quote: ... The following primary antibodies were diluted 1:400 in blocking buffer and incubated over night at 4°C: mouse anti-dsRNA J2 (RNT-SCI-10010200; Jena bioscience), mouse anti-DDX5 (67025 Proteintech ...
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bioRxiv - Immunology 2020Quote: ... Vero E6 cells were incubated with a 1:500 dilution of an anti-dsRNA J2 antibody (Jena Bioscience) in PBS supplemented with 1% FCS at 4°C overnight with shaking ...
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bioRxiv - Neuroscience 2020Quote: ... Endogenous and overexpressed PML was immunofluorescently labelled with anti-PML antibody (rabbit, ABD-030, Jena Bioscience, Germany, 1:500), followed by secondary antibody coupled with STAR 580 STED dye (goat-anti-rabbit ...
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Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translationbioRxiv - Biochemistry 2024Quote: ... 50 μl of the blank agarose bead control slurry and 50 μl of the γ-aminophenyl-m7GTP agarose C10-linked bead slurry (Jena Biosciences) were taken to separate 1.5 ml microcentrifuge tubes ...
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bioRxiv - Biochemistry 2020Quote: ... Additives as indicated were added during the primary antibody incubation step at a final concentration of 1 µM for adenosine (Jena Bioscience), AMP (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... anti-dsRNA monoclonal antibody (Jena Bioscience, Germany ...
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bioRxiv - Biochemistry 2023Quote: ... 0.9 mL of DNase solution (15 mM MgSO4, 1 mM DTT and 1 mM PMSF, 1 x Turbo DNase (Jena biosciences)) was added and the samples were allowed to shake at 37 °C for 10 min before transferring to ice ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with SiR-Tet (1–2 µM, 1 h; Spirochrome, Stein am Rhein, Switzerland) or TAMRA-Tet (2 µM, 1 h; Jena BioScience, Germany) and washed again with fresh medium (3 × quick wash and 3 × 30 min wash ...
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bioRxiv - Molecular Biology 2023Quote: ... Each fraction was then incubated with anti-dsRNA monoclonal antibody J2 (SCICONS/Jena Bioscience RNT-SCI-10010200 ...
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bioRxiv - Cell Biology 2019Quote: ... 12 μM unlabeled tubulin + 1 μM Alexa-568 tubulin + 1 μM biotin tubulin was polymerized in BRB80 (80 mM Pipes, 1 mM EGTA, 1 mM MgCl2) in the presence of 1 mM GMPCPP (Jena Bioscience, NU-405L) for 1 h at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM GTP (Roth, K056.4) or 1 mM GMP (Jena Biosciences, JBS-NU-1028), was added to the washed beads and incubated gently by rocking for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM GMPCPP (Jena Biosciences) and 4 mM MgCl2 ...
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bioRxiv - Cell Biology 2020Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GMPCPP (Jena Bioscience, Jena, Germany). The polymerized microtubules were centrifuged for 30 min at 18000 x g in a Microfuge 18 Centrifuge (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GMPCPP (Jena Bioscience, Jena, Germany). The polymerized microtubules were centrifuged for 30 min at 18.000 x g in a Microfuge 18 Centrifuge (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... and 1 μL of 3-azido-7-hydroxycoumarin solution (1 mM in EtOH; Jena Bioscience) was added ...
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bioRxiv - Neuroscience 2024Quote: ... pH 6.9) supplemented with 1 mM MgCl2 and 1 mM GTP (Jena Bioscience, Jena, Germany) for 30 minutes at 37 °C prior to centrifugation at 18.000 x g for 30 min in a Microfuge 18 Centrifuge (Beckman Coulter ...
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bioRxiv - Biophysics 2021Quote: ... 1 mM GMPCPP (Jena Bioscience, Germany), 1 mM MgCl2 and 4 mg mL-1 of porcine brain tubulin ...
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bioRxiv - Cell Biology 2021Quote: ... with 1 mM GMPCPP (Jena Bioscience) in PEM80 (80 mM Pipes ...
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bioRxiv - Biophysics 2023Quote: ... and Wizard 1&2 (Jena Bioscience) screens were used at 16 °C ...
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bioRxiv - Biochemistry 2023Quote: ... and 1 mM GTP (Jena Bioscience). A 12.5 µM solution of fluorescently labeled tubulin in 1x assay buffer containing an oxygen scavenger system (freshly mixed 0.035 mg/ml catalase (Sigma Aldrich) ...