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Citations for Cytoskeleton :
1 - 50 of 101 citations for Zika Virus Vero Cell Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cell lysate (125 μg) was added to 5 μg GGA3-PBD beads (Cytoskeleton, USA) to pull down ARFs-GTP and incubated at 4°C on a rotator for 1h and then the beads were extensively washed to remove unbound protein ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were then used for G-LISA small GTPase activation assay (Cytoskeleton cat# BK135), where levels of activated RhoA ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were harvested as outlined in the G-lisa RhoA Activation Assay Kit (Cytoskeleton Inc) using the supplied lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were lysed and protein lysates were used for G-LISA analysis per manufacturer’s (Cytoskeleton) instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cells lysates were prepared according to manufacturer’s instructions (RhoA Pull-Down Activation Assay Biochem Kit; Cytoskeleton Inc., BK036). Specifically ...
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bioRxiv - Cell Biology 2023Quote: ... GTP-bound ARF6 was detected in cell lysates using the ARF6 Activation Assay Biochem Kit™ (Cytoskeleton, BK-033) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
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bioRxiv - Neuroscience 2023Quote: ... Cleared lysates were incubated with Rhotekin-Rho binding domain (RBD) beads (60 µg; Cytoskeleton Inc.) or with PAK-p21 binding domain (PBD ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration of the cleared lysates was determined by Precision Red Advanced Protein Assay Reagent (Cytoskeleton Inc.). Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: RHOA activity was measured on LN-F lysates using the RHOA G-LISA Activation Assay kit (#BK124, Cytoskeleton). Data are the mean ± SEM of arbitrary units of RHOA activity.
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bioRxiv - Cell Biology 2021Quote: ... Lysates were spun at maximum speed for 10mins before protein level was measured using Precision Red (Cytoskeleton, Inc., #ADV02). Samples were run using precast 4-12% NuPAGE Bis-Tris Acrylamide Gels (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volumes of G-actin and F-actin lysates were analysed by SDS-PAGE and immunoblotting with anti-actin rabbit polyclonal antibody (Cat# AANO1, Cytoskeleton). Densitometric analyses of the G-actin and F-actin levels were performed using ImageJ software ...
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bioRxiv - Neuroscience 2023Quote: F- and G-actin fractions from lysates were prepared using a G-Actin/F-Actin in Vivo Assay Biochem kit (Cytoskeleton, #BK037) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Western blots to determine the ratio of G-actin to F-actin were performed on retinal lysates that were processed using a G-actin/F-actin In Vivo Assay Kit (Cytoskeleton, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Active GTP-bound GTPase levels in brain lysates were measured using G-LISA activation assay kits for Rac1 (Cytoskeleton, Inc., BK128) and RhoA (Cytoskeleton ...
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bioRxiv - Biochemistry 2024Quote: ... Normalized Lysates were incubated with 20μL of anti-FLAG affinity gel or anti-Acetyl-Lysine (mAb mix) Affinity Beads (Cytoskeleton, Inc., AAC04-beads) at 4°C for 2 h ...
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bioRxiv - Microbiology 2022Quote: ... live-cell SiR-actin (Cytoskeleton, Inc.) was added to the complete medium at the basal side ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were imaged with Citifluor (Cytoskeleton Inc.) anti-bleaching agent ...
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bioRxiv - Cell Biology 2022Quote: ... cells stained with Flipper-TR (Cytoskeleton, CY-SC020) were excited using a 488nm pulsed laser with a modulation frequency of 20MHz and imaged through a 595/40nm bandpass filter followed by MPD APD detectors ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with MemGlow-488 (Cytoskeleton Inc.) at 200nM to label the plasma membrane.
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bioRxiv - Cell Biology 2023Quote: ... Cells were treated with SiR-DNA (Cytoskeleton Inc.), plated and imaged in 4-well cover glass dishes (Cellvis) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were pre-incubated with 50nM siR-actin (Cytoskeleton) for 1hr prior to trypsinization and immediate transfer to an imaging chamber ...
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bioRxiv - Immunology 2020Quote: ... cells were treated with C3 transferase (Cytoskeleton Inc., #CT04) 1.5 μg/ml for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: Cell tracing dyes including SPY555-Actin (Cytoskeleton CY-SC202), SPY650-Tubulin (Cytoskeleton CY-SC503 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were overlaid with SIR-Actin (Cytoskeleton, CY-SC001) at a final concentration of 1 µM in FluoroBrite DMEM containing 10% FBS and incubated for 1 h ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were incubated with 25 nM SiR-Tubulin (Cytoskeleton Inc.) for 4 h prior to imaging ...
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bioRxiv - Developmental Biology 2020Quote: ... YAP (Cell 1:200, Signaling) or Phalloidin (3.5:500, Cytoskeleton). Immunodetection was performed with either rhodamine or fluorescein-conjugated anti-mouse or anti-rabbit secondary antibody diluted in blocking solution ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated with 1ug/ul C3 transferase (Cytoskeleton, Inc.) diluted in serum-free media for 4 hours ...
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bioRxiv - Cell Biology 2023Quote: NK cells were labelled with SPY650-DNA (Cytoskeleton, CY-SC501) and Cellbrite Steady 550 with Cellbrite Enhancer reagent (Biotium ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with 1 µm sirDNA (Cytoskeleton, CY-SC007) and 1 µm verapamil (Cytoskeleton ...
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bioRxiv - Cell Biology 2021Quote: ... Splenic B-cells were preloaded with SiR-Lysosome (1 µM, Cytoskeleton) in the presence of verapamil (10 µM ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with 100 nM rhodamine-phalloidin (Cytoskeleton Inc, #PHDR1) in blocking buffer for 30 minutes at RT in the dark ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated with 1:100 rhodamine phalloidin (# PHDR1, Cytoskeleton Inc.) for 60 min at room temperature.
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bioRxiv - Immunology 2020Quote: ... or 1 μM of the Cell permeant C3 transferase (from Cytoskeleton).
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bioRxiv - Biophysics 2022Quote: ... COS-7 cells were incubated with SiR-Tubulin (Cytoskeleton, CY-SC002) for ~20 mins before imaging without washing.
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bioRxiv - Cell Biology 2024Quote: ... Cell nuclei were visualized via SPY650-DNA (Cytoskeleton, cat. #: CY-SC501) probe that was incubated for 2 hours at 37° C at a 1:1,000 dilution in complete media ...
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bioRxiv - Cancer Biology 2024Quote: ... we stained cells with SPY555-actin (1:1,000; Cytoskeleton CY-SC202) at 37 °C for 1 hr ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell-permeable C3 transferase-based Rho inhibitor 1 was from Cytoskeleton. Saracatinib and defactinib were from Selleck Chemicals ...
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bioRxiv - Bioengineering 2019Quote: ... transfected cells were stained by SiR-DNA nuclear staining kit (Cytoskeleton. Inc) for 2 hours before imaging ...
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bioRxiv - Cell Biology 2021Quote: ... WT Caco-2 cells were treated with 1ug/ml CN03 (Cytoskeleton, Inc) or 1μg/ml C3 Transferase (Cytoskeleton ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with 1 μM SiR-DNA (Cytoskeleton, Inc. CY-SC007) for 1 hour prior to imaging and kept in SiR-DNA-containing live imaging media during imaging ...
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bioRxiv - Biochemistry 2022Quote: ... Finally cells were stained with Actin-Stain 488 Phalloidin (1:1000; Cytoskeleton) and with 2 µg/ml Hoechst 33342 (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... dissociated cells were stained with SiR-DNA (1µM, Cytoskeleton Inc., CY-SC007) and CellTracker Green CMFDA Dye (2.5µg/ml ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated in culture medium containing SiR-Tubulin (Spirochrome/Cytoskeleton Inc.) for 1 hr at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... cells were pre-extracted for 5 minutes with cold CSK buffer (‘Cytoskeleton buffer’ ...
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bioRxiv - Neuroscience 2023Quote: ... cells were treated with 1 μM Sir-Actin (Cytoskeleton, Denver, CO, USA) for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were labeled by adding the dyes MemGlow 488 (Cytoskeleton; 1:200), SPY555-Actin (Cytoskeleton ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were labeled with the dyes SPY555-Actin (Cytoskeleton; 1:1,000) and NucSpot Live 650 (Biotium ...
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bioRxiv - Biophysics 2021Quote: ... Cells were incubated overnight with 100nM SiR-Tubulin (Cytoskeleton, Inc.; Cat.#CY-SC002), then seeded at high density onto micropatterned coverslips (same as for immunofluorescence ...
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bioRxiv - Biophysics 2021Quote: Cells were incubated with 1-2 μM of SiR-Actin (Cytoskeleton, Inc. Spirochrome) for 15-30 min in calcium-free buffer ...