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Citations for Cytoskeleton :
101 - 143 of 143 citations for Warfarin Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Pharmacological treatments included 1 unit/ml Rho Activator I (CN01; Cytoskeleton, Denver, CO), 1 mM TAK165 (Mubritinib ...
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bioRxiv - Cell Biology 2020Quote: Microtubule polymerization assays were performed using 1.5 mg/mL purified tubulin (Cytoskeleton, Inc) that was taken from a 10 mg/mL flash frozen stock ...
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bioRxiv - Neuroscience 2020Quote: ... Starved flies were then moved to tubes with standard corn meal and sugar cane syrup supplemented with taxol (10 nM, 100 nM, 1 µM or 10 µM, Paclitaxel Cytoskeleton Inc.) or DMSO (0.01% ...
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bioRxiv - Cell Biology 2022Quote: Prophase I-arrested oocytes were cultured in vitro in milrinone-free CZB medium supplemented with 100 nM SiR-tubulin (Cytoskeleton #NC0958386) in a humidified ...
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bioRxiv - Biophysics 2022Quote: ... then coated with 6μg/ml of Rhodamine Fibronectin (Cat. # FNR01, CYTOSKELETON, Denver, CO, USA) by incubating at 37°C for 1hr ...
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bioRxiv - Biochemistry 2023Quote: ... microtubules were polymerized from 5 mg mL-1 porcine tubulin (Cytoskeleton Inc., Denver, CO) at 37 °C for 20 min in BRB80 supplemented with 1 mM GTP and then diluted 1:10 in BRB80 + 20 µM taxol ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then washed three times in PBS and stored in PBS containing SIR-actin (100 nM; Cytoskeleton; catalogue number: CY-SC001) at 4°C until imaging ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then washed three times in PBS and stored in PBS containing SiR-actin (100 nM; Cytoskeleton; catalog number: CY-SC001) at 4°C until imaging ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed three times in PBS and stored in PBS containing SiR-actin (100 nM; Cytoskeleton; catalog number: CY-SC001) at 4°C until imaging ...
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bioRxiv - Cell Biology 2021Quote: ... Porcine brain microtubules were prepared from frozen aliquots of 5mg/ml tubulin (Cytoskeleton, Denver, CO) in polymerization buffer (BRB80 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then permeabilized for 10 minutes with 0.1% Triton X-100 and stained with Rhodamine Phalloidin for 30 minutes (Cytoskeleton Inc., Denver, CO, USA). Cells were then washed in DPBS and mounted with DAPI-containing Vectashield mounting media (Vector Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... tubulin was labeled by adding 100 nM SiR-tubulin and 10 µM verapamil for 30-60 minutes prior to imaging (Cytoskeleton, Inc. CY-SC002). Actin was labeled by diluting SPY555-actin (Cytoskeleton ...
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bioRxiv - Cell Biology 2020Quote: ... and then placed in 1.5 ml imaging media containing 1 μM SiR-DNA (CY-SC007, Cytoskeleton) + 10 μM Verapamil (for nucleus staining ...
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bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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bioRxiv - Cell Biology 2022Quote: ... Microtubules were made by mixing 1 μl of HiLyte Fluor 488 tubulin (2 mg/ml, Cytoskeleton), 2 μl biotinylated tubulin (2 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: Microtubules were made by mixing 1.5 μL of 2 mg/mL HiLyte Fluor 488 tubulin (Cytoskeleton), 2 μL of 2 mg/mL ...
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bioRxiv - Biophysics 2023Quote: ... we performed additional tests: incubation with 1.0 μg/ml C3-transferase (RhoA inhibitor, Cytoskeleton, cat. CT04) and with 1.0 μg/ml of RhoA activator (Cytoskeleton ...
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bioRxiv - Biophysics 2021Quote: ... 0.2 mg ml−1 κ-casein and) lacking taxol but including 1 μM SiR-tubulin (Cytoskeleton inc.) and the mixture was allowed to bind to coverslips for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... 0.2 μl of 1 mg/ml HiLyte Fluor 647 or X-rhodamine-labelled tubulin (#TL670M, #TL6290M, Cytoskeleton) and 0.5 μl of 0.5 mg/ml biotin tubulin (#T333P ...
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bioRxiv - Cell Biology 2021Quote: Fluorescent MTs were polymerized at 2 mg/ml by incubating tubulin and rhodamine labeled tubulin (Cytoskeleton Inc.) at 10:1 ratio in BRB80 with 1 mM DTT and 1mM GTP for 5 min on ice ...
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bioRxiv - Biophysics 2021Quote: ... for 24 hours and treated with 5ng/ml TGFβ1 and 100nM SiR-actin kit (Cytoskeleton.Inc, CY-SC001).
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bioRxiv - Biophysics 2019Quote: ... the primary antibodies used in the study were sheep anti-tubulin (2.5 μg mL−1, ATN02, Cytoskeleton) and mouse anti-TOM20 (2.5 μg mL−1 ...
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bioRxiv - Cell Biology 2020Quote: A solution of 5 μg/mL Fibronectin labeled with HiLyte fluor 488 (FNR02-A, Cytoskeleton, Inc. USA) in PBS was incubated on glass bottom petri dish for 30 min at 4°C and rinsed with cold PBS thrice ...
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bioRxiv - Biophysics 2023Quote: ... They are next incubated with a 10 μg/mL rhodamine-labelled fibronectin solution (Cytoskeleton, cat. FNR01-A) for 1h and then dried at room temperature for about 5min ...
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bioRxiv - Cell Biology 2023Quote: Stock actin (10 mg/ml) was diluted 1:10 into ATP-supplemented general actin buffer from Cytoskeleton Inc ...
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bioRxiv - Bioengineering 2023Quote: ... we incubated the glass coverslip with a 500 µg/mL solution of green fluorescent laminin (Cytoskeleton, Inc., LMN02) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Gels were then incubated with a 10 μg/ml laminin solution (1:1 rhodamine labeled (LMN01-A, Cytoskeleton, Inc.) to non-labeled laminin-1 (L2020 ...
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bioRxiv - Cell Biology 2023Quote: ... a reaction vessel was prepared on ice containing 3 mg/ml of >97% purified porcine tubulin (Cytoskeleton, Inc. (#T240)) and the substances being tested ...
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bioRxiv - Molecular Biology 2021Quote: ... the pathway was first inhibited with fasudil 50µM (Bio-Techne, Minnesota, USA) or C3-exoenzyme 2µg/ml (Cytoskeleton, Denver, USA) for 15 hours and then activated with PDGF-AA at 50ng/ml for 20 minutes and proceed to the G-LISA or IF assay of myosin light chain phosphorylation (p-MLC) ...
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bioRxiv - Molecular Biology 2021Quote: ... the pathway was activated with PDGF-AA at 50 ng/ml for 20 minutes at 37°C and the G-LISA assay (Cytoskeleton) was performed following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... of each was incubated for 15 min at 37°C in General Tubulin Buffer with 2.25 mg/mL of 99% pure porcine tubulin and 1 mM GTP (all from Cytoskeleton Inc.), in a final volume of 50 μL ...
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bioRxiv - Cell Biology 2022Quote: ... the medium was replaced by a standard growth medium supplemented with 2% (vol/vol) GFR Matrigel and 10 µg/ml of a fluorescently labeled ECM molecule (HiLyte Fluor 488-labeled fibronectin, Cytoskeleton, Cat Number FNR02 ...
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bioRxiv - Bioengineering 2022Quote: ... The beads were cleaned 3 times in PBS and resuspended in a solution of PBS with 20 μg/ml biotinylated fibronectin (Cytoskeleton) or biotinylated BSA (Pierce ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were cultured for nine days and fluorescent labeling of matrices for direct visualization during live cell microscopy was achieved by adding 1 µg/ml rhodamine-coupled fibronectin (Cytoskeleton) at day three ...
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bioRxiv - Biochemistry 2023Quote: ... was added to either supplemented actin buffer alone or in the presence of 0.4 mg mL-1 G-actin (cat. AKL99, Cytoskeleton Inc.). The final ligand concentration was 2.5 μM ...
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bioRxiv - Cell Biology 2023Quote: ... The coverslips were then rinsed in water and coated with 20 µg/mL fibronectin (1/10 Rhodamine Fibronectin (Cytoskeleton, FNR01), 9/10 fibronectin (FC010 ...
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bioRxiv - Biophysics 2019Quote: ... This is done by mixing 5 μL sonicated GMPCPP microtubule (1 mg/mL short microtubules prepared from 97% pure tubulin (HTS03-A, Cytoskeleton, Inc), stored at −80 °C and thawed right before use ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... the samples were preincubated for either 3 h with Rho/Rac/Cdc42 Activator I (1 µg/ml, Catalog number CN04, Cytoskeleton Inc), 30 min with MEK inhibitor PD 98059 (50 µM ...
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bioRxiv - Biophysics 2022Quote: ... the microstamps were inked for 1 hour at room temperature with 25 μg/mL rhodamine FN solution (Cytoskeleton Inc., Colorado, United States). Deformable silicone Flexwell membranes (Flexcell inc. ...
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bioRxiv - Cell Biology 2020Quote: ... cells were grown at high density (150,000 cells/cm2) for 48 hours and were then treated with Rho-activator-II (Cytoskeleton: 1 µg/ml) for 3 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... Fixed gelatin-coated coverslips were washed six times with CMF-PBS at room temperature and coated with 50 µg/ml HiLyte488 FITC-conjugated fibronectin (Cytoskeleton, Inc., Denver, CO) for one hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... embryos were washed three times with PBS and stained with 2 μg/mL Hoechst 33342 (Nacalai Tesque, 04929-82) and 20 nM Acti-stain™ 555 phalloidin (Cytoskeleton, PHDH1-A) in PBS containing 0.05% Triton X-100 at 4°C overnight in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended in DMEM-1%BSA and incubated in suspension for 30 min at 37°C in 10% CO2 and 2 μg/ml CT04 (#CT04-A, Cytoskeleton or 10 μM EHT1864 (#3872, Tocris). The vehicle control included the corresponding dilution of DMSO as needed ...