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Citations for Cytoskeleton :
101 - 150 of 162 citations for Testosterone 100 Ug Ml In Methylene Chloride 3 4 13C2 99%; 17 18O 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... 1.0 µg/mL of RhoA inhibitor C3 transferase (Cytoskeleton, Cat# CT04) for 16 hours ...
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bioRxiv - Cell Biology 2021Quote: ... 22 μl of 5 mg ml−1 tubulin protein (Cytoskeleton, T240) was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton ...
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bioRxiv - Biophysics 2023Quote: ... and with 1.0 μg/ml of RhoA activator (Cytoskeleton, cat. CN03). Drugs led to decrease and increase of activities respectively (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... and 1 μg ml-1 for Rho activator II (CNO3, Cytoskeleton). Cells were treated at least 2h at 37 °C and 5% CO2 ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... Small GTPases (Rho/Rac/Cdc42) activator CN04 (Cytoskeleton, Inc.; 1μg/mL).
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... Rho inhibitor I (Cytoskeleton, CT04-A, used at 2 μg/ml), Y-27632 (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... actomyosin contractility of MECs stably expressing an inducible ROCK construct was induced overnight using 4-hydroxytamoxifen and the actin cortex of these cells was labelled with SiR-actin dye (Cytoskeleton) according to manufacturer’s instructions prior to laser ablation.
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected upon 15min of centrifugation at >15000 rpm at 4°C and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). Equal amounts of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: Mouse antibodies against Myc-tag (M185-3L/M047-3, MBL, Nagoya, Japan), rabbit antibody against GFP-tag (D110008, BBI, Shanghai, China) and sheep antibody against Tubulin (ATN02, Cytoskeleton, Denver, United States) were subjected to Western blot according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... microtubules were labeled by incubating cells with 100 nM SiR-tubulin and 10 µM verapamil (CY-SC002, Cytoskeleton Inc.) for 60 min prior to imaging ...
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bioRxiv - Cell Biology 2021Quote: ... WT Caco-2 cells were treated with 1ug/ml CN03 (Cytoskeleton, Inc) or 1μg/ml C3 Transferase (Cytoskeleton ...
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bioRxiv - Cell Biology 2022Quote: ... 0.4 µl of 5 mg/ml biotin labeled tubulin (Cytoskeleton, #T333P-A), 20% glycerol ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 μL of 10 mg/mL unlabeled tubulin (Cytoskeleton Inc., #T240) was mixed on ice ...
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bioRxiv - Cell Biology 2022Quote: ... The following inhibitors were used: C3 exoenzyme (1 mg/ml; Cytoskeleton, Inc), NSC23766 (50 mM ...
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bioRxiv - Biophysics 2019Quote: MTs were prepared by polymerizing 5 mg/ml tubulin (Cytoskeleton, Denver, CO) in BRB80 buffer (80 mM PIPES ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 ng/mL doxycycline and 50 nM Sir-DNA (Cytoskeleton, CY-SC007). Cells were incubated for 24 hours prior to imaging ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: Dynamic MT seed assays were performed by polymerizing GMPCPP stabilized Cy5-MT seeds from 4 µM HiLyte 647 tubulin (Cytoskeleton, #TL670M) in BD buffer + 0.5 mM GMPCPP at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed three times and incubated with SiR-actin (100 nM in PBS; Cytoskeleton; catalog number: CY-SC001) at 4°C until imaging (minimum length of staining ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then washed three times and incubated with SiR-actin (100 nM in PBS; Cytoskeleton; catalog number: CY-SC001) at 4°C until imaging (minimum length of staining ...
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bioRxiv - Biophysics 2023Quote: ... cell clusters on Day 7 were incubated overnight with 100 nM Acti-stainTM 488 Phalloidin (Cat. #PHDG1, Cytoskeleton Inc., USA) and washed thrice with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then washed three times in PBS and stored in PBS containing SiR-actin (100 nM; Cytoskeleton; CY-SC001) at 4°C until imaging ...
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bioRxiv - Microbiology 2020Quote: ... The treatment consisted of 2 µg/ml of Rho inhibitor I (Cytoskeleton, CT04) for 2 h or 20 µM of Y27632 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Pharmacological treatments included 1 unit/ml Rho Activator I (CN01; Cytoskeleton, Denver, CO), 1 mM TAK165 (Mubritinib ...
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bioRxiv - Cell Biology 2020Quote: Microtubule polymerization assays were performed using 1.5 mg/mL purified tubulin (Cytoskeleton, Inc) that was taken from a 10 mg/mL flash frozen stock ...
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bioRxiv - Neuroscience 2020Quote: ... Starved flies were then moved to tubes with standard corn meal and sugar cane syrup supplemented with taxol (10 nM, 100 nM, 1 µM or 10 µM, Paclitaxel Cytoskeleton Inc.) or DMSO (0.01% ...
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bioRxiv - Cell Biology 2022Quote: Prophase I-arrested oocytes were cultured in vitro in milrinone-free CZB medium supplemented with 100 nM SiR-tubulin (Cytoskeleton #NC0958386) in a humidified ...
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bioRxiv - Biophysics 2022Quote: ... then coated with 6μg/ml of Rhodamine Fibronectin (Cat. # FNR01, CYTOSKELETON, Denver, CO, USA) by incubating at 37°C for 1hr ...
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bioRxiv - Biochemistry 2023Quote: ... microtubules were polymerized from 5 mg mL-1 porcine tubulin (Cytoskeleton Inc., Denver, CO) at 37 °C for 20 min in BRB80 supplemented with 1 mM GTP and then diluted 1:10 in BRB80 + 20 µM taxol ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then washed three times in PBS and stored in PBS containing SIR-actin (100 nM; Cytoskeleton; catalogue number: CY-SC001) at 4°C until imaging ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then washed three times in PBS and stored in PBS containing SiR-actin (100 nM; Cytoskeleton; catalog number: CY-SC001) at 4°C until imaging ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed three times in PBS and stored in PBS containing SiR-actin (100 nM; Cytoskeleton; catalog number: CY-SC001) at 4°C until imaging ...
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bioRxiv - Cell Biology 2021Quote: ... Porcine brain microtubules were prepared from frozen aliquots of 5mg/ml tubulin (Cytoskeleton, Denver, CO) in polymerization buffer (BRB80 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then permeabilized for 10 minutes with 0.1% Triton X-100 and stained with Rhodamine Phalloidin for 30 minutes (Cytoskeleton Inc., Denver, CO, USA). Cells were then washed in DPBS and mounted with DAPI-containing Vectashield mounting media (Vector Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... tubulin was labeled by adding 100 nM SiR-tubulin and 10 µM verapamil for 30-60 minutes prior to imaging (Cytoskeleton, Inc. CY-SC002). Actin was labeled by diluting SPY555-actin (Cytoskeleton ...
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bioRxiv - Cell Biology 2020Quote: ... and then placed in 1.5 ml imaging media containing 1 μM SiR-DNA (CY-SC007, Cytoskeleton) + 10 μM Verapamil (for nucleus staining ...
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bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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bioRxiv - Cell Biology 2022Quote: ... Microtubules were made by mixing 1 μl of HiLyte Fluor 488 tubulin (2 mg/ml, Cytoskeleton), 2 μl biotinylated tubulin (2 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: Microtubules were made by mixing 1.5 μL of 2 mg/mL HiLyte Fluor 488 tubulin (Cytoskeleton), 2 μL of 2 mg/mL ...
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bioRxiv - Biophysics 2023Quote: ... we performed additional tests: incubation with 1.0 μg/ml C3-transferase (RhoA inhibitor, Cytoskeleton, cat. CT04) and with 1.0 μg/ml of RhoA activator (Cytoskeleton ...
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bioRxiv - Biophysics 2021Quote: ... 0.2 mg ml−1 κ-casein and) lacking taxol but including 1 μM SiR-tubulin (Cytoskeleton inc.) and the mixture was allowed to bind to coverslips for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... 0.2 μl of 1 mg/ml HiLyte Fluor 647 or X-rhodamine-labelled tubulin (#TL670M, #TL6290M, Cytoskeleton) and 0.5 μl of 0.5 mg/ml biotin tubulin (#T333P ...
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bioRxiv - Cell Biology 2021Quote: Fluorescent MTs were polymerized at 2 mg/ml by incubating tubulin and rhodamine labeled tubulin (Cytoskeleton Inc.) at 10:1 ratio in BRB80 with 1 mM DTT and 1mM GTP for 5 min on ice ...
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bioRxiv - Biophysics 2021Quote: ... for 24 hours and treated with 5ng/ml TGFβ1 and 100nM SiR-actin kit (Cytoskeleton.Inc, CY-SC001).
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bioRxiv - Biophysics 2019Quote: ... the primary antibodies used in the study were sheep anti-tubulin (2.5 μg mL−1, ATN02, Cytoskeleton) and mouse anti-TOM20 (2.5 μg mL−1 ...
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bioRxiv - Cell Biology 2020Quote: A solution of 5 μg/mL Fibronectin labeled with HiLyte fluor 488 (FNR02-A, Cytoskeleton, Inc. USA) in PBS was incubated on glass bottom petri dish for 30 min at 4°C and rinsed with cold PBS thrice ...
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bioRxiv - Biophysics 2023Quote: ... They are next incubated with a 10 μg/mL rhodamine-labelled fibronectin solution (Cytoskeleton, cat. FNR01-A) for 1h and then dried at room temperature for about 5min ...
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bioRxiv - Cell Biology 2023Quote: Stock actin (10 mg/ml) was diluted 1:10 into ATP-supplemented general actin buffer from Cytoskeleton Inc ...
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bioRxiv - Bioengineering 2023Quote: ... we incubated the glass coverslip with a 500 µg/mL solution of green fluorescent laminin (Cytoskeleton, Inc., LMN02) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Gels were then incubated with a 10 μg/ml laminin solution (1:1 rhodamine labeled (LMN01-A, Cytoskeleton, Inc.) to non-labeled laminin-1 (L2020 ...