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Citations for Cytoskeleton :
51 - 81 of 81 citations for Suppressor of CDC2 With RNA Binding Motif 2 RBMS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... moderate amounts of anti-ac-K antibody agarose beads (Cytoskeleton, Inc., USA) were added to the lysate and incubated for 12 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: Fluorescent MTs were polymerized at 2 mg/ml by incubating tubulin and rhodamine labeled tubulin (Cytoskeleton Inc.) at 10:1 ratio in BRB80 with 1 mM DTT and 1mM GTP for 5 min on ice ...
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bioRxiv - Biochemistry 2020Quote: Biotin-inactivated myosin was prepared by reacting 2 mg of rabbit skeletal muscle myosin (Cytoskeleton Cat # MYO2) with 5 μL of 250 mM EZ-Link-Maleimide-PEG11-Biotin dissolved in DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... 2 mM EGTA) supplemented with 4.51% of sucrose to adjust osmotic pressure (Cytoskeleton buffer with sucrose; CBS) and then fixed in 3% PFA in CBS for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies used were as follows: mouse anti-RAC1 (Cytoskeleton #ARC03, 1:250); mouse anti-RHOA (Cytoskeleton #ARH05 ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used: rabbit anti-cofilin (Cytoskeleton ACFL02; 1:500), rabbit anti-Ser3-phosphorylated cofilin (Cell Signaling Technology #3311 ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies used were mouse anti-Rac1 (1:500, Cytoskeleton Inc, #ARC03) and rabbit anti-GAPDH (1:3000 ...
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bioRxiv - Microbiology 2023Quote: ... the samples were washed 2× with IF buffer and incubated with rhodamine phalloidin (PHDR1) (1:500) (Cytoskeleton, Inc.) for 30 minutes in the dark at 4 °C ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were then stimulated with vehicle or 100ng/ml EGF for 15 minutes or 2 hours and lysed with Cell Lysis Buffer (50mM Tris pH 7.5, 10mM MgCl2, 0.5M NaCl, 2% Igepal) (Cytoskeleton Inc.) containing protease and phoshatase inhibitors (Pierce/Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... 2% BSA) prior to immunostaining for 20 min with a 1:500 dilution of Acti-stain-555 (Cytoskeleton Inc). The ratio of cytoplasmic actin to cortical actin was measured using ImageJ (Fiji 2.1.0/1.53c ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed 2 times with 1xPBS and then incubated with rhodamine phalloidin (PHDR1; 1:500) (Cytoskeleton Inc.) in IF wash buffer for 30 min at RT in the dark ...
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bioRxiv - Microbiology 2022Quote: Proteins were collected in cell lysis buffer (50mM Tris pH 7.5, 10mM MgCl2, 0.5M NaCl, 2% Igepal) supplemented with 1X Protease inhibitor cocktail (Cytoskeleton, PIC02). Lysates were cleared by 1min centrifugation at 10,000g at 4°C and the collected supernatants were snap-frozen in liquid nitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... phenol-red free DMEM and incubated with MemGlow dyes (MemGlow 560, MG02-2 and MemGlow 590, MG03-10; Cytoskeleton) in serum-free ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used included anti-β-actin (1:1,000, AAN01, Cytoskeleton, Inc., Denver, CO, USA), anti-AMPKα (1:1,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Rabbit polyclonal antibody recognizing the Kif11/Eg5 protein (Cat. #AKIN03, discontinued) was purchased from Cytoskeleton, Inc ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated with monoclonal mouse anti-human RhoA primary antibody (# GL01A; 1:250; Cytoskeleton Inc.), followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02 ...
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bioRxiv - Cell Biology 2019Quote: ... followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02; 1:62.5; Cytoskeleton Inc.) on a shaker at 300 rpm at room temperature ...
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bioRxiv - Cell Biology 2020Quote: We used the following monoclonal antibodies: mouse anti-ubiquitin clone P4D1 (Cytoskeleton, Inc, Denver, Co, USA), mouse anti-β-actin clone BA3R (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was probed using the iBind flex kit and a primary anti-RhoA antibody (Cytoskeleton) at 1:200 and HRP-conjugated secondary (1:4000) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were analyzed by western blot using anti-ubiquitin antibody (P4D1, Cytoskeleton AUB01-HRP, 1:1000) and visualized with Clarity Western ECL Substrate (BioRad).
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bioRxiv - Cell Biology 2020Quote: ... Immunoblots were performed using the following primary antibodies: rabbit anti-KIF15 (1:1000; Cytoskeleton Inc., Cat#AKIN13), rabbit anti-KIF2A (1:1000 ...
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bioRxiv - Biophysics 2019Quote: ... the primary antibodies used in the study were sheep anti-tubulin (2.5 μg mL−1, ATN02, Cytoskeleton) and mouse anti-TOM20 (2.5 μg mL−1 ...
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bioRxiv - Biophysics 2022Quote: ... The membrane was then probed with a mouse monoclonal anti-RhoA antibody (supplied with the Cytoskeleton Kit) at a working dilution of 1:500 overnight at 4 °C with constant agitation ...
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bioRxiv - Neuroscience 2021Quote: ... p-ERK1/2 (CST, 9101, 1:1000), AKT (CST, 9272, 1:1000), p-AKT (CST, 9275, 1:1000) and Cofilin (Cytoskeleton, ACFL02, 1:500) were used to quantify protein levels ...
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bioRxiv - Cell Biology 2022Quote: ... embryos were washed three times with PBS and stained with 2 μg/mL Hoechst 33342 (Nacalai Tesque, 04929-82) and 20 nM Acti-stain™ 555 phalloidin (Cytoskeleton, PHDH1-A) in PBS containing 0.05% Triton X-100 at 4°C overnight in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... and secondary antibody at 1 h (4 °C) facilitated by an automated western blot liquid handler (Cytoskeleton GOBlot). Antibodies utilised included anti-eIF2α (abcam ...
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bioRxiv - Bioengineering 2023Quote: ... Lipids accumulated in HLOs and cytoskeleton were stained with 2 μM BODIPY® 493/503 and 1 μM SiR-Actin (Cytoskeleton Inc., CO, USA), respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... F-actin and G-actin were quantified by SDS-PAGE and western blot using actin antibody (#BK037, Cytoskeleton Inc.).
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bioRxiv - Cell Biology 2022Quote: ... Equal volumes of G-actin and F-actin lysates were analysed by SDS-PAGE and immunoblotting with anti-actin rabbit polyclonal antibody (Cat# AANO1, Cytoskeleton). Densitometric analyses of the G-actin and F-actin levels were performed using ImageJ software ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended in DMEM-1%BSA and incubated in suspension for 30 min at 37°C in 10% CO2 and 2 μg/ml CT04 (#CT04-A, Cytoskeleton or 10 μM EHT1864 (#3872, Tocris). The vehicle control included the corresponding dilution of DMSO as needed ...
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bioRxiv - Neuroscience 2020Quote: ... probed with the rabbit anti-myosin 18A polyclonal antibody directed against the C-terminal 18 residues of myosin 18Aα/β (1:1000, cite JB Cytoskeleton 2017) and with a mouse anti-RACK1 monoclonal antibody (1:1000 ...