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Citations for Cytoskeleton :
51 - 79 of 79 citations for Siglec 10 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Taxol-stabilized microtubules assembled from unlabeled and fluorescently-labeled porcine tubulin (10:1 ratio; Cytoskeleton) were introduced into the chamber ...
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bioRxiv - Biophysics 2020Quote: ... and 0.1-0.25 nM rabbit skeletal actin filaments polymerized with 10-15% biotinylated actin (Cytoskeleton) and stabilized by rhodamine-phalloidin (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... Taxol-stabilized microtubules assembled from unlabeled and fluorescently-labeled porcine tubulin (10:1 ratio; Cytoskeleton) were introduced into the chamber ...
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bioRxiv - Neuroscience 2020Quote: Taxol-treated cultures were incubated with 10 nM taxol dissolved in DMSO (4h, Paclitaxel Cytoskeleton Inc.) in plating medium ...
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bioRxiv - Microbiology 2021Quote: ... and infected with MVA-E5R-mCherry at MOI 10 and stained with fluorogenic SiR-DNA (Cytoskeleton). Cells were incubated at 37°C supplemented with 5% CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained with 1 µM SiR-actin and 10 µM verapamil (Cytoskeleton, Inc., CY-SC001) for 1-3 hours before imaging ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PBS was exchanged for PBS containing the DNA stain Hoechst and sir-actin (10 µM; Cytoskeleton, Inc), which is used for live samples ...
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bioRxiv - Biochemistry 2022Quote: Dolastatin-10 rings (D-rings) were made by incubating 80 µM porcine brain tubulin (Cytoskeleton, Inc. CO) with 160 µM dolastatin-10 (APExBIO ...
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bioRxiv - Biophysics 2023Quote: ... They are next incubated with a 10 μg/mL rhodamine-labelled fibronectin solution (Cytoskeleton, cat. FNR01-A) for 1h and then dried at room temperature for about 5min ...
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bioRxiv - Immunology 2023Quote: ... Some TFF-concentrated EVs were fluorescently labeled with 200 nM MemGlow 700 nm dye (Cytoskeleton, catalog #MG05-10) for 30 min at room temperature (RT ...
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bioRxiv - Neuroscience 2021Quote: ... washed and the immunoprecipitated resolved on 12% SDS-PAGE and detected via Western blot analysis using Cdc42 (mouse anti-Cdc42, ACD03 Cytoskeleton; 1:250), Rac1 (mouse anti-Rac1 ...
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bioRxiv - Cell Biology 2022Quote: ... Gels were then incubated with a 10 μg/ml laminin solution (1:1 rhodamine labeled (LMN01-A, Cytoskeleton, Inc.) to non-labeled laminin-1 (L2020 ...
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bioRxiv - Cell Biology 2021Quote: ... microtubules were labeled by incubating cells with 100 nM SiR-tubulin and 10 µM verapamil (CY-SC002, Cytoskeleton Inc.) for 60 min prior to imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... phenol-red free DMEM and incubated with MemGlow dyes (MemGlow 560, MG02-2 and MemGlow 590, MG03-10; Cytoskeleton) in serum-free ...
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bioRxiv - Physiology 2020Quote: Recombinant tau from bacteria or heat-stable tau fractions isolated from mouse brains or SH-SY5Y cells stably expressing tau were incubated with 30 μM porcine brain tubulin (Cytoskeleton, Denver, CO, USA) in PEM buffer (80 mM PIPES ...
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bioRxiv - Cell Biology 2021Quote: ... GMPCPP-stabilized microtubule seeds were assembled using 50 μM porcine tubulin containing 1:12 biotin-labeled and 1:10 rhodamine-labeled porcine tubulins (Cytoskeleton) and polymerized in the presence of 1mM GMPCPP (Jena Bioscience ...
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bioRxiv - Cell Biology 2021Quote: ... Tubulin was labeled in 1:10 proportions with biotin or X-rhodamine according to published protocols or mixed with prelabeled tubulin purchased from Cytoskeleton, Inc.
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bioRxiv - Cell Biology 2022Quote: ... the medium was replaced by a standard growth medium supplemented with 2% (vol/vol) GFR Matrigel and 10 µg/ml of a fluorescently labeled ECM molecule (HiLyte Fluor 488-labeled fibronectin, Cytoskeleton, Cat Number FNR02 ...
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bioRxiv - Cell Biology 2023Quote: ... The coverslips were then rinsed in water and coated with 20 µg/mL fibronectin (1/10 Rhodamine Fibronectin (Cytoskeleton, FNR01), 9/10 fibronectin (FC010 ...
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bioRxiv - Molecular Biology 2022Quote: ... or 60 min-old Tau:polyA or Tau:PEG:polyA condensates containing 10% Cy3-labeled polyA were imaged prior and after the addition of 5 μM tubulin (bovine cycled tubulin, PurSolutions-PUR-032005) containing 10% HiLyte647-labeled tubulin (porcine tubulin, Cytoskeleton-TL670M). For tubulin bundle formation ...
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bioRxiv - Cell Biology 2021Quote: ... Chromosomes were labeled in the inducible DHC-KO cell line (Figures 1C, 1I, 1J) by incubating cells in 1 µM SiR-DNA and 10 µM verapamil (CY-SC007, Cytoskeleton Inc.) for 60 min prior to imaging ...
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bioRxiv - Cell Biology 2024Quote: ... Soluble proteins were collected after a 10 minute centrifugation step at 15000 rpm and protein concentration was measured using PrecisionRed (Cytoskeleton; ADV02). 1.5 mg of protein was mixed with 25 μl of pre-equilibrated GFP-Trap_A beads (ChromoTek ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated for 30 min with 0.5 μM of SiR-actin or 60 min with 0.5 μM of SiR-tubulin and 10 μM of verapamil in culture medium (SiR Cytoskeleton Kit, Spirochrome SC006).
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bioRxiv - Biophysics 2021Quote: ... labeled tubulin with 9 μL unlabeled MTs and transferred to a 37°C water bath for 20 min and stabilized by adding 10 μM paclitaxel (Cytoskeleton, Denver, CO). MTs were stored at room temperature ...
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bioRxiv - Biophysics 2022Quote: BJ-5ta cells were seeded on RGD-54pN or LDVP-54pN surfaces for 40 min and 20 nM SiR-actin and 10 µM verapamil (Cytoskeleton, CY-SC001) were added 20 min before imaging ...
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bioRxiv - Biophysics 2023Quote: ... the cells were incubated in cell culture media containing 10 nM of the 647 (HTL-PA-JF647) and a 500x dilution manufacturer recommend stock concentration of SPY505 (Cytoskeleton, CY-SC101) for 1 hour ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then permeabilized for 10 minutes with 0.1% Triton X-100 and stained with Rhodamine Phalloidin for 30 minutes (Cytoskeleton Inc., Denver, CO, USA). Cells were then washed in DPBS and mounted with DAPI-containing Vectashield mounting media (Vector Laboratories ...
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bioRxiv - Cell Biology 2020Quote: Microtubules were polymerized from cycled bovine tubulin (PurSolutions—Nashville, TN) mixed with Cy5-labled bovine tubulin (PurSolutions) and stabilized with 10 μM Taxol (Cytoskeleton Inc.—Denver, CO) as previously described (71) ...
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bioRxiv - Cell Biology 2023Quote: ... tubulin was labeled by adding 100 nM SiR-tubulin and 10 µM verapamil for 30-60 minutes prior to imaging (Cytoskeleton, Inc. CY-SC002). Actin was labeled by diluting SPY555-actin (Cytoskeleton ...