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Citations for Cytoskeleton :
101 - 142 of 142 citations for Saporin Chicken Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... a solution containing filaments of 0.3 nM TMR-phalloidin-labeled biotinylated actin (Cytoskeleton, Denver, CO, USA), 0.4% glucose ...
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bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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bioRxiv - Cell Biology 2019Quote: ... followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02; 1:62.5; Cytoskeleton Inc.) on a shaker at 300 rpm at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... F-actin filaments and the nuclei were labeled with 1 µM of SiR-actin (Cytoskeleton, Denver, USA) and 1 µg/ml of Hoechst 33342 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Microtubule growth was initiated by flowing in 10.7 μM tubulin containing 7% Hilyte647–labeled tubulin (Cytoskeleton Inc.) together with 36 nM motor proteins in the reaction buffer (BRB80 with 1 mM GTP ...
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bioRxiv - Cell Biology 2021Quote: Fluorescent MTs were polymerized at 2 mg/ml by incubating tubulin and rhodamine labeled tubulin (Cytoskeleton Inc.) at 10:1 ratio in BRB80 with 1 mM DTT and 1mM GTP for 5 min on ice ...
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bioRxiv - Cell Biology 2020Quote: A solution of 5 μg/mL Fibronectin labeled with HiLyte fluor 488 (FNR02-A, Cytoskeleton, Inc. USA) in PBS was incubated on glass bottom petri dish for 30 min at 4°C and rinsed with cold PBS thrice ...
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bioRxiv - Biophysics 2021Quote: ... Labeled MTs were generated by mixing 4μL of 20 μM Cy5 (PurSolutions) or Rhodamine (Cytoskeleton, Denver, CO) labeled tubulin with 9 μL unlabeled MTs and transferred to a 37°C water bath for 20 min and stabilized by adding 10 μM paclitaxel (Cytoskeleton ...
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bioRxiv - Cell Biology 2023Quote: ... Mitotic chromosomes were labeled with 0.2 μM SiR-DNA in live cells during imaging (Cytoskeleton, CY-SC007).
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bioRxiv - Developmental Biology 2023Quote: ... 1 μg tubulin purified from porcine brain (Cytoskeleton, T240) was mixed with 0.5 μg/μl BSA in assay buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... MT affinity/dead-head spin was performed by binding motor protein to newly polymerized MTs (unlabeled, 2uM Paclitaxel (Taxol (Cytoskeleton, Denver, CO, US, TXD01) -stabilized ...
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bioRxiv - Cell Biology 2019Quote: ... Microtubule growth was then initiated by flowing in 20 μM tubulin containing 7% Hilyte647-labeled tubulin (Cytoskeleton Inc.) together with cell extracts in the reaction buffer [2.5 mM GTP ...
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bioRxiv - Immunology 2023Quote: ... Some TFF-concentrated EVs were fluorescently labeled with 200 nM MemGlow 700 nm dye (Cytoskeleton, catalog #MG05-10) for 30 min at room temperature (RT ...
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bioRxiv - Cell Biology 2023Quote: ... biotin-and HiLyte-488-labeled pig brain microtubules were polymerized in vitro using commercial tubulin sources (Cytoskeleton Inc.) and stabilized with taxol (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μM purified rabbit skeletal muscle actin (#AKL99-C, Cytoskeleton) was diluted in general actin buffer (5 mM Tris pH 7.4 ...
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bioRxiv - Plant Biology 2019Quote: ... The purified Arp2/3 protein complex from porcine brain (Cytoskeleton) and GST-tagged VCA domain of human WASP protein (Cytoskeleton ...
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bioRxiv - Cell Biology 2020Quote: ... Imaging solution consisted of indicated concentration of porcine brain tubulin (5% or 15% rhodamine-labeled, Cytoskeleton Inc., Denver CO) and Alexa488-labeled tau in BRB80 supplemented with 1 mM GTP ...
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bioRxiv - Cell Biology 2022Quote: ... Gels were then incubated with a 10 μg/ml laminin solution (1:1 rhodamine labeled (LMN01-A, Cytoskeleton, Inc.) to non-labeled laminin-1 (L2020 ...
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bioRxiv - Cell Biology 2020Quote: ... Actin (2µM; 7% pyrene-labeled) was then polymerized in the presence of 20nM bovine Arp2/3 complex (Cytoskeleton Inc.) plus MBP-tagged fusion proteins in control buffer supplemented with 0.2mM ATP and 0.5mM DTT ...
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bioRxiv - Cell Biology 2021Quote: GMPCPP-stabilized microtubules were assembled using 50 μM unlabeled porcine tubulin containing 1:12 biotin-labeled porcine tubulin (Cytoskeleton) and polymerized in the presence of 1mM GMPCPP (Jena Bioscience ...
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bioRxiv - Cell Biology 2021Quote: ... Seeds were formed by shearing larger MTs (GMPCPP-stabilized, labeled 1:40 with HiLyte 488 tubulin (Cytoskeleton, TL488M-B) with a Hamilton syringe (Avanti Polar Lipids ...
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bioRxiv - Cell Biology 2022Quote: ... This tubulin was then mixed with both biotin-conjugated and HiLyte 647 labeled porcine tubulin (Cytoskeleton Inc., Denver, CO). The tubulin mix was then resuspended in PEM buffer (80 mM PIPES pH 6.9 ...
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bioRxiv - Biophysics 2020Quote: ... 10% rhodamine-labeled F-actin was prepared by co-polymerizing 0.9 μM unlabeled actin monomers and 0.1 μM rhodamine-labeled actin monomers (Cytoskeleton, AR05) in the presence of G-Mg (2 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... microtubules were labeled by incubating cells with 100 nM SiR-tubulin and 10 µM verapamil (CY-SC002, Cytoskeleton Inc.) for 60 min prior to imaging ...
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bioRxiv - Cell Biology 2022Quote: ... before incubating for 5 minutes at room temperature in 40 ng/µl rhodamine-labeled actin (Cat# AR05, Cytoskeleton, Inc.) freshly prepared in cytoskeletal buffer ...
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bioRxiv - Biochemistry 2021Quote: Porcine Tubulin at 1:5 ratio (rhodamine-labeled: unlabeled) (cat. no. T240 and TL590M-A respectively, Cytoskeleton, Inc., Denver, USA) was reconstituted to 4.0 mg/mL in cold polymerization buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volumes of G-actin and F-actin lysates were analysed by SDS-PAGE and immunoblotting with anti-actin rabbit polyclonal antibody (Cat# AANO1, Cytoskeleton). Densitometric analyses of the G-actin and F-actin levels were performed using ImageJ software ...
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bioRxiv - Molecular Biology 2022Quote: ... or 60 min-old Tau:polyA or Tau:PEG:polyA condensates containing 10% Cy3-labeled polyA were imaged prior and after the addition of 5 μM tubulin (bovine cycled tubulin, PurSolutions-PUR-032005) containing 10% HiLyte647-labeled tubulin (porcine tubulin, Cytoskeleton-TL670M). For tubulin bundle formation ...
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bioRxiv - Cell Biology 2023Quote: ... The tubulin was then mixed with both biotin-conjugated and HiLyte 647 (far-red) labeled porcine tubulin (Cytoskeleton Inc., Denver, CO) and resuspended in PEM buffer (80 mM PIPES pH 6.9 ...
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bioRxiv - Cell Biology 2022Quote: ... the purified His-MBP-NRIPs were incubated with F-actin (AD99, Cytoskeleton) in 1 ml of IP buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: Colchicine-competition assays were performed with 2 μM purified tubulin (Cytoskeleton, Inc) taken from a 10 mg/mL stock prepared in tubulin buffer as above ...
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bioRxiv - Cell Biology 2020Quote: Microtubule polymerization assays were performed using 1.5 mg/mL purified tubulin (Cytoskeleton, Inc) that was taken from a 10 mg/mL flash frozen stock ...
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bioRxiv - Neuroscience 2020Quote: ... probed with the rabbit anti-myosin 18A polyclonal antibody directed against the C-terminal 18 residues of myosin 18Aα/β (1:1000, cite JB Cytoskeleton 2017) and with a mouse anti-RACK1 monoclonal antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... tubulin was labeled by adding 100 nM SiR-tubulin and 10 µM verapamil for 30-60 minutes prior to imaging (Cytoskeleton, Inc. CY-SC002). Actin was labeled by diluting SPY555-actin (Cytoskeleton ...
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bioRxiv - Plant Biology 2020Quote: ... 13 nM Arp2/3 protein complex and 15 nMWASp-VCA domain-GST purified (Cytoskeleton, USA) along with freshly purified ArF-BAR protein in concentrations of 200 ...
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bioRxiv - Neuroscience 2023Quote: ∼30µg of purified recombinant TDP-43 and 10µg of rabbit skeletal muscle actin (Cytoskeleton, #BK037) were incubated with Ni-NTA beads at 4°C overnight on a rotating wheel ...
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bioRxiv - Cell Biology 2024Quote: ... Microtubules were polymerized in vitro from purified tubulin dimers (Cytoskeleton, Inc,; Zeng et al., 2010). To evaluate whether CAPS coprecipitated with polymerized microtubules ...
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bioRxiv - Plant Biology 2019Quote: ... The seven protein subunits of the Arp2/3 protein complex were purified from bovine brain (Cytoskeleton). The GST-VCA fusion protein associated with the glutathione-agarose 4B (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... purified tubulin from bovine brain was utilized in the present study (catalog No. T238, Cytoskeleton, Denver, CO). Tubulin was polymerized with a “hybrid” protocol in the absence of taxol ...
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bioRxiv - Cell Biology 2021Quote: ... Different types of labelled and unlabeled purified tubulin used in the assays were either purchased from Cytoskeleton or purified as described previously (57 ...
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bioRxiv - Cell Biology 2023Quote: ... a reaction vessel was prepared on ice containing 3 mg/ml of >97% purified porcine tubulin (Cytoskeleton, Inc. (#T240)) and the substances being tested ...
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bioRxiv - Biochemistry 2022Quote: ... The integrated signal measured from the area of the dynein bands is extracted using ImageJ and calibrated using known masses of purified dynein protein (CS-DN01, Cytoskeleton, Denver, CO, USA) that serve as standards ...