Labshake search
Citations for Cytoskeleton :
101 - 121 of 121 citations for SARS CoV 2 Spike E M Mosaic Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... 2 mM EGTA) supplemented with 4.51% of sucrose to adjust osmotic pressure (Cytoskeleton buffer with sucrose; CBS) and then fixed in 3% PFA in CBS for 15 min ...
-
bioRxiv - Developmental Biology 2022Quote: ... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
-
bioRxiv - Neuroscience 2020Quote: ... In vitro microtubule-binding assay was performed using the microtubule-binding protein spin down assay kit (Cytoskeleton, Denver, CO, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... F-actin and G-actin protein samples were collected according to the manufacturer’s instructions (#BK037, Cytoskeleton Inc., Denver, CO, USA). F-actin and G-actin were quantified by SDS-PAGE and western blot using actin antibody (#BK037 ...
-
bioRxiv - Cell Biology 2020Quote: Muscle homogenates were prepared in buffer A as described above and an equal amount of protein from each sample was incubated with 20 µL of Rhotekin-RBD beads (Cytoskeleton) for 2 hours at 4°C and washed 4 times in ice-cold buffer containing 50 mM Tris (pH 7.4) ...
-
bioRxiv - Cell Biology 2022Quote: ... and Rac or Rho activity was assessed in duplicate using the G-LISA small G-protein activation assay kit (BK128, BK124, Cytoskeleton) according to the manufacturer’s directions ...
-
bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected upon 15min of centrifugation at >15000 rpm at 4°C and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). Equal amounts of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
-
bioRxiv - Microbiology 2023Quote: ... the samples were washed 2× with IF buffer and incubated with rhodamine phalloidin (PHDR1) (1:500) (Cytoskeleton, Inc.) for 30 minutes in the dark at 4 °C ...
-
bioRxiv - Systems Biology 2020Quote: ... Cells were then stimulated with vehicle or 100ng/ml EGF for 15 minutes or 2 hours and lysed with Cell Lysis Buffer (50mM Tris pH 7.5, 10mM MgCl2, 0.5M NaCl, 2% Igepal) (Cytoskeleton Inc.) containing protease and phoshatase inhibitors (Pierce/Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... 2% BSA) prior to immunostaining for 20 min with a 1:500 dilution of Acti-stain-555 (Cytoskeleton Inc). The ratio of cytoplasmic actin to cortical actin was measured using ImageJ (Fiji 2.1.0/1.53c ...
-
bioRxiv - Microbiology 2022Quote: ... The cells were washed 2 times with 1xPBS and then incubated with rhodamine phalloidin (PHDR1; 1:500) (Cytoskeleton Inc.) in IF wash buffer for 30 min at RT in the dark ...
-
bioRxiv - Microbiology 2022Quote: Proteins were collected in cell lysis buffer (50mM Tris pH 7.5, 10mM MgCl2, 0.5M NaCl, 2% Igepal) supplemented with 1X Protease inhibitor cocktail (Cytoskeleton, PIC02). Lysates were cleared by 1min centrifugation at 10,000g at 4°C and the collected supernatants were snap-frozen in liquid nitrogen ...
-
bioRxiv - Cancer Biology 2024Quote: ... phenol-red free DMEM and incubated with MemGlow dyes (MemGlow 560, MG02-2 and MemGlow 590, MG03-10; Cytoskeleton) in serum-free ...
-
bioRxiv - Microbiology 2019Quote: ... 5 μL of the filtered supernatant was pipetted into a clear flat-bottom UV plate and 250 μL of 1x Advanced Protein Assay Reagent (Cytoskeleton Inc., CO) was added and mixed ...
-
bioRxiv - Biochemistry 2022Quote: ... The integrated signal measured from the area of the dynein bands is extracted using ImageJ and calibrated using known masses of purified dynein protein (CS-DN01, Cytoskeleton, Denver, CO, USA) that serve as standards ...
-
bioRxiv - Cell Biology 2021Quote: ... MT affinity/dead-head spin was performed by binding motor protein to newly polymerized MTs (unlabeled, 2uM Paclitaxel (Taxol (Cytoskeleton, Denver, CO, US, TXD01) -stabilized ...
-
bioRxiv - Neuroscience 2021Quote: ... p-ERK1/2 (CST, 9101, 1:1000), AKT (CST, 9272, 1:1000), p-AKT (CST, 9275, 1:1000) and Cofilin (Cytoskeleton, ACFL02, 1:500) were used to quantify protein levels ...
-
bioRxiv - Cell Biology 2022Quote: ... embryos were washed three times with PBS and stained with 2 μg/mL Hoechst 33342 (Nacalai Tesque, 04929-82) and 20 nM Acti-stain™ 555 phalloidin (Cytoskeleton, PHDH1-A) in PBS containing 0.05% Triton X-100 at 4°C overnight in the dark ...
-
bioRxiv - Biophysics 2021Quote: ... the cells were rinsed thoroughly and stained with 1:200 solution of secondary antibody in PBS for 2 hours followed by staining with Acti-Stain™ 670 Fluorescent Phalloidin (Cytoskeleton.Inc, catalog no. PHDN1). Then the cover-slides were mounted on glass slides using ProLong Diamond Antifade Mountant (Invitrogen ...
-
bioRxiv - Bioengineering 2023Quote: ... Lipids accumulated in HLOs and cytoskeleton were stained with 2 μM BODIPY® 493/503 and 1 μM SiR-Actin (Cytoskeleton Inc., CO, USA), respectively ...
-
bioRxiv - Cell Biology 2022Quote: ... resuspended in DMEM-1%BSA and incubated in suspension for 30 min at 37°C in 10% CO2 and 2 μg/ml CT04 (#CT04-A, Cytoskeleton or 10 μM EHT1864 (#3872, Tocris). The vehicle control included the corresponding dilution of DMSO as needed ...