Labshake search
Citations for Cytoskeleton :
1 - 50 of 118 citations for Recombinant Red Fluorescent Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
-
bioRxiv - Plant Biology 2019Quote: ... and GST-tagged VCA domain of human WASP protein (Cytoskeleton) were used to initiate actin nucleation ...
-
bioRxiv - Developmental Biology 2023Quote: ... Protein quantification was performed using Precision Red Protein Assay Reagent (Cytoskeleton) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined using Precision Red (Cytoskeleton) and 60μg of protein were separated using 4-15% SDS-PAGE gels and transferred onto nitrocellulose membrane ...
-
bioRxiv - Cancer Biology 2022Quote: ... Precision Red Advanced Protein Assay (ADV02-A; Cytoskeleton), calcein AM (C1430 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Protein concentration was quantified using Precision Red (Cytoskeleton) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... Protein concentration was determined using Precision Red Advanced Protein Assay (Cytoskeleton, Inc.). 20ug protein were loaded onto 8-12% SDS-PAGE gels and transferred onto nitrocellulose membranes ...
-
bioRxiv - Cell Biology 2022Quote: ... or along with the human recombinant proteins: WASP-VCA domain protein (Cat# VCG03, Cytoskeleton) or ERK3 protein (M31-34G ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentration was measured with Precision Red Assay (Cytoskeleton). Laemmli sample buffer (Biorad ...
-
bioRxiv - Cancer Biology 2022Quote: ... Protein was quantified using the Precision Red (#ADV02; Cytoskeleton) advanced protein assay and 10-20μg was used ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined using the Precision Red Advanced Protein Assay Reagent (Cytoskeleton ADV02). Extracted proteins (25-35 μg ...
-
bioRxiv - Cell Biology 2019Quote: ... and protein concentration determined using the Precision Red™ Advanced Protein Assay Reagent (Cytoskeleton). Respective samples were treated with 300nM Nocodazole for 15 min before lysis ...
-
bioRxiv - Cell Biology 2023Quote: ... and total protein concentration was measured using Precision Red (Cytoskeleton). Normalized samples were denatured with SDS loading buffer (250mM Tris [pH 6.8] ...
-
bioRxiv - Neuroscience 2020Quote: ... and protein concentration estimated in the supernatant using Precision Red Advanced Protein Assay (Cytoskeleton, CA). Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad) ...
-
bioRxiv - Plant Biology 2019Quote: The VCA domain of human WASP protein was expressed in a bacterial expression system as a GST-tagged fusion protein (Cytoskeleton). The seven protein subunits of the Arp2/3 protein complex were purified from bovine brain (Cytoskeleton) ...
-
bioRxiv - Cell Biology 2020Quote: ... reserving a small amount for protein quantification using Precision Red (Cytoskeleton). Prior to measuring GTPase activity ...
-
bioRxiv - Cell Biology 2019Quote: ... and protein concentrations were measured using the Precision Red Advanced Protein Assay Reagent (# ADV02; Cytoskeleton Inc.) and finally equalized with ice-cold lysis buffer to 1.0 mg/ml ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein concentration of the cleared lysates was determined by Precision Red Advanced Protein Assay Reagent (Cytoskeleton Inc.). Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen ...
-
bioRxiv - Biochemistry 2023Quote: ... and protein concentrations were estimated using the Precision Red reagent (Cytoskeleton Inc). Protein samples were prepared by mixing 1X LDS (Invitrogen) ...
-
bioRxiv - Biophysics 2024Quote: ... GST-tagged WCA domain of human WASP protein (GST-WCA) was purchased from Cytoskeleton (Cat. #VCG03-A) and used without further purification ...
-
bioRxiv - Cell Biology 2022Quote: ... Pyrene-labeled actin was incubated with recombinant ARP2/3 protein complex alone (Cat# RP01P, Cytoskeleton) or along with the human recombinant proteins ...
-
Gα13 loss promotes tumor progression in the KPC transgenic mouse model of advanced pancreatic cancerbioRxiv - Cancer Biology 2021Quote: ... and the protein concentration was determined using Precision Red solution (Cytoskeleton, Inc., Denver, CO) according to the manufacturer’s instructions ...
-
bioRxiv - Pathology 2023Quote: ... and the protein concentration was determined using Precision Red solution (Cytoskeleton, Inc., Denver, CO). Equal amounts of protein were separated with a 10% SDS-PAGE electrophoresis gel ...
-
bioRxiv - Microbiology 2019Quote: ... Whole colonic tissue was homogenized in cell lysis buffer and protein concentration was determined using the Precision Red Protein Assay (Cytoskeleton). Samples were sent to Eve Technologies for analysis using their 32-multiplex Mouse Cytokine/Chemokine Array.
-
bioRxiv - Cell Biology 2021Quote: ... Lysates were spun at maximum speed for 10mins before protein level was measured using Precision Red (Cytoskeleton, Inc., #ADV02). Samples were run using precast 4-12% NuPAGE Bis-Tris Acrylamide Gels (ThermoFisher Scientific ...
-
bioRxiv - Developmental Biology 2022Quote: ... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
-
bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected upon 15min of centrifugation at >15000 rpm at 4°C and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). Equal amounts of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
-
bioRxiv - Cell Biology 2023Quote: ... Precision Red (Cytoskeleton ADV02-A) was used to quantify protein amount in RIPA lysate ...
-
bioRxiv - Bioengineering 2020Quote: ... Acti-stain™ Fluorescent Phalloidin (Cytoskeleton, Inc.) and DAPI (Sigma ...
-
bioRxiv - Cell Biology 2021Quote: ... Green Fluorescent Fibronectin (Cytoskeleton, Inc HiLyte 488) was used to determine gel functionalisation in Supplementary Fig ...
-
bioRxiv - Microbiology 2021Quote: ... Actin-stain 488 (Red, Cytoskeleton Inc.) and DAPI (Blue ...
-
bioRxiv - Biochemistry 2024Quote: ... and His-DBS (DH/PH domain) were purchased from Cytoskeleton, Inc ...
-
bioRxiv - Cell Biology 2021Quote: ... The laminin density was evaluated by coating the dish with laminin conjugated to a fluorescent dye (green fluorescent HiLyte 488; Cytoskeleton), which was observed under a confocal laser scanning microscope (Zeiss LSM710 ...
-
bioRxiv - Neuroscience 2019Quote: ... Actin-stain 555 Fluorescent Phalloidin was from Cytoskeleton. The siRNA against CaMKIIγ was from GenePharma (Shanghai ...
-
bioRxiv - Developmental Biology 2024Quote: ... or Acti-stain 488 fluorescent Phalloidin (Cytoskeleton, Inc.) in PBST for 20 min ...
-
bioRxiv - Biochemistry 2023Quote: ... rhodamine- and Hi-Lyte647-labelled porcine tubulin were purchased from Cytoskeleton (T240 ...
-
bioRxiv - Cancer Biology 2023Quote: ... NCI-H295R cells were fluorescently tagged using MemGlow 560 (Cytoskeleton, Inc., Denver, CO) following manufacturer instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... the purified His-MBP-NRIPs were incubated with F-actin (AD99, Cytoskeleton) in 1 ml of IP buffer (50 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2021Quote: ... and 6% fluorescent (X-Rhodamine or HiLyte647) tubulin (Cytoskeleton Inc) in the presence of the nonhydrolyzable GTP analogue GMPCPP (Jena Bioscience ...
-
bioRxiv - Bioengineering 2023Quote: ... The COS-7 cells were labelled with PKmito RED (Nanjing Genvivo Biotech Co., Ltd. PKMR-1, CY-SC052)/PKmito DEEP RED (Cytoskeleton, CY-SC055) 15 minutes in DMEM ...
-
bioRxiv - Cell Biology 2022Quote: ... Solutions of 434 nM His-CLASP2α or 1 μM α-actinin (Cytoskeleton, Inc.) were incubated with and without 15 μM phalloidin-stabilized F-actin for 20 minutes at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... and with different recombinant Cdc42 (3µg of human Cdc42, #CD01 Cytoskeleton; 3µg of recombinant MBP tagged-C ...
-
bioRxiv - Microbiology 2023Quote: ... 100 nM Acti-stain 488 Fluorescent Phalloidin (Cytoskeleton, Denver, CO, USA), and 300 nM DAPI (Merck ...
-
bioRxiv - Microbiology 2023Quote: ... and ∼5% Alexa488-labeled or Alexa647-labeled fluorescent tubulin (Cytoskeleton inc.) were prepared as described64 and stabilized with final 20µM taxol (Cytoskeleton inc.) ...
-
bioRxiv - Cell Biology 2022Quote: ... taxol-stabilized microtubules and recombinant human kinesin (KIF5A) were purchased from Cytoskeleton™ (Denver ...
-
bioRxiv - Molecular Biology 2020Quote: ... and incubated with Acti-Stain 670 fluorescent phalloidin (Cytoskeleton #PHDG1, 70 nM) and 4’,6-diamidino-2-phenylindole (DAPI ...
-
bioRxiv - Cell Biology 2022Quote: ... Taxol-stabilized microtubules with ∼10% biotin-tubulin and ∼10% fluorescent-tubulin (Cytoskeleton) were prepared as described previously51 ...
-
bioRxiv - Cell Biology 2020Quote: To analyze the competition between actin and His-Rap1b (Cytoskeleton Inc, cat. no. RR02-A) biding to GST SPN some modifications were made to the assay ...
-
bioRxiv - Cell Biology 2022Quote: ... osteoclasts were incubated with live imaging compatible fluorescent probes: SIR-Actin (Cytoskeleton, Inc.), LysoTrackerTM Red DND-99 (Thermo Fisher) ...
-
bioRxiv - Cell Biology 2019Quote: ... media was replaced with Fluorobright fluorescent imaging media containing 500 nM sirDNA (Cytoskeleton). The chamber was then transferred to a Nikon Ti microscope fitted with an OKOlab environmental control chamber ...