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Citations for Cytoskeleton :
101 - 150 of 204 citations for Recombinant Human GPC3 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... This tubulin was mixed with both biotin-conjugated and rhodamine labeled porcine tubulin (Cytoskeleton Inc.). The tubulin mix was then resuspended in PEM buffer (80 mM PIPES pH 6.9 ...
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bioRxiv - Cell Biology 2023Quote: ... Polymerization assays were performed using a mixture of commercial rhodamine labeled actin monomers (Cytoskeleton, Inc.) and unlabeled actin monomers that were mixed at a 1:1 ratio ...
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bioRxiv - Cell Biology 2023Quote: Porcine brain tubulin (T240) and rhodamine-labeled tubulin (TL590M) were purchased from Cytoskeleton (Denver, CO). Guanosine-5’-[(α,β)-methyleno] triphosphate (GMPCPP ...
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bioRxiv - Cell Biology 2023Quote: ... Porcine brain tubulin (T240) and rhodamine-labeled tubulin (TL590M) were purchased from Cytoskeleton (Denver, CO). Guanosine-5’-[(α,β)-methyleno] triphosphate (GMPCPP ...
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bioRxiv - Biophysics 2023Quote: ... F-actin was labeled with rhodamine-phalloidin (PHDR1; Cytoskeleton, Inc., Denver, CO, USA; 1:167).
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bioRxiv - Cell Biology 2024Quote: ... the cilia of cultured multiciliated cells were live-labeled using SiR-tubulin (Cytoskeleton, #CY-SC002) or Spy555-tubulin (Cytoskeleton ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentration was determined using Precision Red Advanced Protein Assay (Cytoskeleton, Inc.). 20ug protein were loaded onto 8-12% SDS-PAGE gels and transferred onto nitrocellulose membranes ...
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bioRxiv - Cell Biology 2021Quote: Microtubules were polymerized (unlabeled and HiLyte-647-labeled porcine brain tubulin, Cytoskeleton Inc #T240 and #TL670M) in BRB80 buffer (80 mM Pipes/KOH pH 6.8 ...
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bioRxiv - Biochemistry 2021Quote: ... a solution containing filaments of 0.3 nM TMR-phalloidin-labeled biotinylated actin (Cytoskeleton, Denver, CO, USA), 0.4% glucose ...
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bioRxiv - Biophysics 2022Quote: ... Lyophilized human cofilin 1 was purchased from Cytoskeleton (CF01) and reconstituted in MB buffer (20 mM MOPS pH 7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... Human full-length gelsolin was purchased from Cytoskeleton (US).
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bioRxiv - Biochemistry 2022Quote: ... The dGAE protein concentration was measured using Advanced Protein Assay Reagent (Cytoskeleton, Inc.) with bovine serum albumin as a standard ...
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bioRxiv - Biophysics 2020Quote: ... F-actin filaments and the nuclei were labeled with 1 µM of SiR-actin (Cytoskeleton, Denver, USA) and 1 µg/ml of Hoechst 33342 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Microtubule growth was initiated by flowing in 10.7 μM tubulin containing 7% Hilyte647–labeled tubulin (Cytoskeleton Inc.) together with 36 nM motor proteins in the reaction buffer (BRB80 with 1 mM GTP ...
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bioRxiv - Cell Biology 2021Quote: Fluorescent MTs were polymerized at 2 mg/ml by incubating tubulin and rhodamine labeled tubulin (Cytoskeleton Inc.) at 10:1 ratio in BRB80 with 1 mM DTT and 1mM GTP for 5 min on ice ...
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bioRxiv - Cell Biology 2020Quote: A solution of 5 μg/mL Fibronectin labeled with HiLyte fluor 488 (FNR02-A, Cytoskeleton, Inc. USA) in PBS was incubated on glass bottom petri dish for 30 min at 4°C and rinsed with cold PBS thrice ...
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bioRxiv - Biophysics 2021Quote: ... Labeled MTs were generated by mixing 4μL of 20 μM Cy5 (PurSolutions) or Rhodamine (Cytoskeleton, Denver, CO) labeled tubulin with 9 μL unlabeled MTs and transferred to a 37°C water bath for 20 min and stabilized by adding 10 μM paclitaxel (Cytoskeleton ...
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bioRxiv - Cell Biology 2023Quote: ... Mitotic chromosomes were labeled with 0.2 μM SiR-DNA in live cells during imaging (Cytoskeleton, CY-SC007).
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bioRxiv - Cell Biology 2023Quote: ... Microtubules were labeled either by injection of HiLyte488-or HiLyte647-tubulin purified from the porcine brain (Cytoskeleton), or recombinant human EB3-mClover protein (a generous gift from So (Nick ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined using the Precision Red Advanced Protein Assay Reagent (Cytoskeleton ADV02). Extracted proteins (25-35 μg ...
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bioRxiv - Cell Biology 2019Quote: ... and protein concentration determined using the Precision Red™ Advanced Protein Assay Reagent (Cytoskeleton). Respective samples were treated with 300nM Nocodazole for 15 min before lysis ...
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bioRxiv - Cell Biology 2020Quote: Purified actin protein (Cytoskeleton, Inc.) was used for the in vitro actin polymerization reaction ...
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bioRxiv - Neuroscience 2020Quote: ... and protein concentration estimated in the supernatant using Precision Red Advanced Protein Assay (Cytoskeleton, CA). Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad) ...
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bioRxiv - Immunology 2023Quote: ... Protein concentrations of crude extracts were determined using the Advanced Protein Assay (Cytoskeleton, Denver, USA).
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bioRxiv - Cell Biology 2019Quote: ... Microtubule growth was then initiated by flowing in 20 μM tubulin containing 7% Hilyte647-labeled tubulin (Cytoskeleton Inc.) together with cell extracts in the reaction buffer [2.5 mM GTP ...
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bioRxiv - Immunology 2023Quote: ... Some TFF-concentrated EVs were fluorescently labeled with 200 nM MemGlow 700 nm dye (Cytoskeleton, catalog #MG05-10) for 30 min at room temperature (RT ...
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bioRxiv - Cell Biology 2023Quote: ... biotin-and HiLyte-488-labeled pig brain microtubules were polymerized in vitro using commercial tubulin sources (Cytoskeleton Inc.) and stabilized with taxol (Sigma Aldrich ...
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bioRxiv - Plant Biology 2019Quote: ... The seven protein subunits of the Arp2/3 protein complex were purified from bovine brain (Cytoskeleton). The GST-VCA fusion protein associated with the glutathione-agarose 4B (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... and protein concentrations were measured using the Precision Red Advanced Protein Assay Reagent (# ADV02; Cytoskeleton Inc.) and finally equalized with ice-cold lysis buffer to 1.0 mg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... Equalized protein extractions were transferred to a Rho-GTP-binding protein pre-coated plate (Cytoskeleton, Inc.). The plate was placed on a microplate shaker at 300 rpm for 30 min at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... C3 exoenzyme protein (CT03; Cytoskeleton, Inc.) was resuspended and dialyzed into PBS and injected at 1 mg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Imaging solution consisted of indicated concentration of porcine brain tubulin (5% or 15% rhodamine-labeled, Cytoskeleton Inc., Denver CO) and Alexa488-labeled tau in BRB80 supplemented with 1 mM GTP ...
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bioRxiv - Cell Biology 2022Quote: ... Gels were then incubated with a 10 μg/ml laminin solution (1:1 rhodamine labeled (LMN01-A, Cytoskeleton, Inc.) to non-labeled laminin-1 (L2020 ...
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bioRxiv - Cell Biology 2020Quote: ... Actin (2µM; 7% pyrene-labeled) was then polymerized in the presence of 20nM bovine Arp2/3 complex (Cytoskeleton Inc.) plus MBP-tagged fusion proteins in control buffer supplemented with 0.2mM ATP and 0.5mM DTT ...
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bioRxiv - Cell Biology 2021Quote: GMPCPP-stabilized microtubules were assembled using 50 μM unlabeled porcine tubulin containing 1:12 biotin-labeled porcine tubulin (Cytoskeleton) and polymerized in the presence of 1mM GMPCPP (Jena Bioscience ...
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bioRxiv - Cell Biology 2021Quote: ... Seeds were formed by shearing larger MTs (GMPCPP-stabilized, labeled 1:40 with HiLyte 488 tubulin (Cytoskeleton, TL488M-B) with a Hamilton syringe (Avanti Polar Lipids ...
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bioRxiv - Cell Biology 2022Quote: ... This tubulin was then mixed with both biotin-conjugated and HiLyte 647 labeled porcine tubulin (Cytoskeleton Inc., Denver, CO). The tubulin mix was then resuspended in PEM buffer (80 mM PIPES pH 6.9 ...
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bioRxiv - Biophysics 2020Quote: ... 10% rhodamine-labeled F-actin was prepared by co-polymerizing 0.9 μM unlabeled actin monomers and 0.1 μM rhodamine-labeled actin monomers (Cytoskeleton, AR05) in the presence of G-Mg (2 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... microtubules were labeled by incubating cells with 100 nM SiR-tubulin and 10 µM verapamil (CY-SC002, Cytoskeleton Inc.) for 60 min prior to imaging ...
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bioRxiv - Cell Biology 2022Quote: ... before incubating for 5 minutes at room temperature in 40 ng/µl rhodamine-labeled actin (Cat# AR05, Cytoskeleton, Inc.) freshly prepared in cytoskeletal buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration of the cleared lysates was determined by Precision Red Advanced Protein Assay Reagent (Cytoskeleton Inc.). Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: Porcine Tubulin at 1:5 ratio (rhodamine-labeled: unlabeled) (cat. no. T240 and TL590M-A respectively, Cytoskeleton, Inc., Denver, USA) was reconstituted to 4.0 mg/mL in cold polymerization buffer ...
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bioRxiv - Genetics 2024Quote: ... Protein quantification was determined via Bradford assay using PrecisionRed Advanced Protein Assay reagent (#ADV01, Cytoskeleton, Denver, CO, USA). Lysate concentration was normalized before dilution in 2X Laemmli Sample buffer with 10% v/v 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2020Quote: Purified tubulin proteins were obtained from Cytoskeleton, Inc ...
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bioRxiv - Molecular Biology 2022Quote: ... or 60 min-old Tau:polyA or Tau:PEG:polyA condensates containing 10% Cy3-labeled polyA were imaged prior and after the addition of 5 μM tubulin (bovine cycled tubulin, PurSolutions-PUR-032005) containing 10% HiLyte647-labeled tubulin (porcine tubulin, Cytoskeleton-TL670M). For tubulin bundle formation ...
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bioRxiv - Cell Biology 2023Quote: ... The tubulin was then mixed with both biotin-conjugated and HiLyte 647 (far-red) labeled porcine tubulin (Cytoskeleton Inc., Denver, CO) and resuspended in PEM buffer (80 mM PIPES pH 6.9 ...
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bioRxiv - Biochemistry 2024Quote: ... Taxol-stabilized human unmodified microtubules assembled with 1.5% biotinylated tubulin (Cytoskeleton, Inc. #T333P) and 1% HiLyte FluorTM 488-labeled tubulin (Cytoskeleton ...
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bioRxiv - Microbiology 2019Quote: ... Whole colonic tissue was homogenized in cell lysis buffer and protein concentration was determined using the Precision Red Protein Assay (Cytoskeleton). Samples were sent to Eve Technologies for analysis using their 32-multiplex Mouse Cytokine/Chemokine Array.
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bioRxiv - Molecular Biology 2020Quote: ... 160 nM WASP VCA domain protein (#VCG03, Cytoskeleton) and 60 nM Arp2/3 protein complex (#RP01P ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined using Precision Red (Cytoskeleton) and 60μg of protein were separated using 4-15% SDS-PAGE gels and transferred onto nitrocellulose membrane ...