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Citations for Cytoskeleton :
1 - 50 of 164 citations for Rat Antigen peptide transporter 2 TAP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Commercial ELISA kits were purchased from Cytoskeleton (G-LISA) to quantify RhoA and Rac1 activation (Cytoskeleton) ...
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bioRxiv - Cancer Biology 2022Quote: RAS activation was assessed by ELISA using a commercially available kit (Cytoskeleton: BK131) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and RAC1 were performed through the use of an ELISA-based Rho-GTPase activation assay kit (Cytoskeleton, Inc, catalog# BK124, BK127, BK128). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... IP of total SUMOylated protein fractions used either SUMO-1 or SUMO-2/3 Ab included with the Signal Seeker SUMOylation 1 or 2/3 Detection Kit (Cytoskeleton) and IPs were carried out according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μl biotinylated tubulin (2 mg/ml, Cytoskeleton) and 7 μl unlabelled pig tubulin (Schlager et al. ...
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bioRxiv - Cell Biology 2020Quote: ... for 2 h or with CT04 (2 μg/ml, Cytoskeleton) for 3 h ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μM unlabeled porcine tubulin (Cytoskeleton) prepared in binding buffer containing 10 mM DTT and 0.2 mM PMSF was added and incubated overnight on a shaker in a cold room ...
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bioRxiv - Cell Biology 2022Quote: ... CT04 (Cytoskeleton; 2 µg/mL; vehicle: dH2O) was added to the media for 2 hours as pre-treatment plus an additional 4 hours with or without Wnt7a.
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bioRxiv - Cancer Biology 2024Quote: ... and 2‰ MemGlow 560 (Fluorogenic Membrane Probe, Cytoskeleton), and imaged on the imaging setup described below.
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bioRxiv - Cell Biology 2022Quote: ... 2 µL of 10 mg/mL unlabeled tubulin (Cytoskeleton) was mixed with 2 µL o f 1 mg/mL biotin-tubulin and 2 µL of 1 mg/mL dye-labeled tubulin on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... or 2 μg/mL Rho A Inhibitor I (Cytoskeleton, Inc.) conditions and imaged immediately after designated incubation time as per commercial protocol (Cytoskeleton Inc.) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... testes were stained with 2 μM SiR-Actin59 (Cytoskeleton, Inc.) for 2-3 hours at room temperature and incubated with Hoechst 33342 (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... 2 μL of 1 mg/mL biotinylated tubulin (Cytoskeleton Inc., #T333P), and 2 μL of 10 mg/mL unlabeled tubulin (Cytoskeleton Inc. ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... Rho inhibitor I (Cytoskeleton, CT04-A, used at 2 μg/ml), Y-27632 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... WT Caco-2 cells were treated with 1ug/ml CN03 (Cytoskeleton, Inc) or 1μg/ml C3 Transferase (Cytoskeleton ...
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bioRxiv - Biophysics 2020Quote: ... Biotin-labeled actin (2 µM) was prepared using 10% biotin actin (Cytoskeleton) in KMg25 buffer ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 μL of 10 mg/mL unlabeled tubulin (Cytoskeleton Inc., #T240) was mixed on ice ...
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bioRxiv - Cell Biology 2020Quote: Colchicine-competition assays were performed with 2 μM purified tubulin (Cytoskeleton, Inc) taken from a 10 mg/mL stock prepared in tubulin buffer as above ...
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bioRxiv - Cell Biology 2021Quote: Actin Polymerization Biochem Kit (Cytoskeleton) was used to investigate F-actin depolymerization by cofilin in the absence or presence of control protein BSA ...
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bioRxiv - Microbiology 2020Quote: ... The treatment consisted of 2 µg/ml of Rho inhibitor I (Cytoskeleton, CT04) for 2 h or 20 µM of Y27632 (Sigma ...
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bioRxiv - Biophysics 2021Quote: Cells were incubated with 1-2 μM of SiR-Actin (Cytoskeleton, Inc. Spirochrome) for 15-30 min in calcium-free buffer ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated 2 hours with C3-transferase (Cytoskeleton Inc., cat. CT03-A) at a concentration of 1μg/mL ...
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bioRxiv - Biophysics 2022Quote: ... provided along with the kit (Cytoskeleton) was used to detect G- and F-actins ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μM rabbit skeletal muscle actin (90% unlabeled and 10% rhodomine-actin) (Cytoskeleton Inc.), 1 μM Shi were incubated in 6.5 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... Cochlea were incubated for 2 minutes at RT in 0.5 µM TMR-actin (Cytoskeleton) diluted in labelling buffer (concentrations in mM) ...
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bioRxiv - Biochemistry 2020Quote: ... provided as a lyophilized powder and resuspended at 2 mM anhydrous DMSO was purchased from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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bioRxiv - Cell Biology 2022Quote: ... Microtubules were made by mixing 1 μl of HiLyte Fluor 488 tubulin (2 mg/ml, Cytoskeleton), 2 μl biotinylated tubulin (2 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: Microtubules were made by mixing 1.5 μL of 2 mg/mL HiLyte Fluor 488 tubulin (Cytoskeleton), 2 μL of 2 mg/mL ...
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bioRxiv - Cancer Biology 2021Quote: Rac1 and RhoA GTPase actitation was measured by G-LISA Rac1 activation assay Kit and G-LISA RhoA activation Kit (Cytoskeleton), according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... assays were carried out using kits from Cytoskeleton, Inc ...
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bioRxiv - Cell Biology 2020Quote: Activated RhoA pulldown kits were purchased from Cytoskeleton. Sample lysates were prepared similar to the protocol used for G-LISAs ...
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bioRxiv - Physiology 2022Quote: ... the Cdc42 activation kit (Cytoskeleton Inc., Denver, USA) was used according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Fluorescent MTs were polymerized at 2 mg/ml by incubating tubulin and rhodamine labeled tubulin (Cytoskeleton Inc.) at 10:1 ratio in BRB80 with 1 mM DTT and 1mM GTP for 5 min on ice ...
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bioRxiv - Biochemistry 2020Quote: Biotin-inactivated myosin was prepared by reacting 2 mg of rabbit skeletal muscle myosin (Cytoskeleton Cat # MYO2) with 5 μL of 250 mM EZ-Link-Maleimide-PEG11-Biotin dissolved in DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... 2 mM EGTA) supplemented with 4.51% of sucrose to adjust osmotic pressure (Cytoskeleton buffer with sucrose; CBS) and then fixed in 3% PFA in CBS for 15 min ...
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bioRxiv - Neuroscience 2022Quote: Rac1 activation was measured by using a Rac1 activation assay kit (Rac1 Pull-down Activation Assay Biochem Kit bead pulldown format, Cytoskeleton, catalog #BK035), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: RAS Activation Assay Biochem Kit™ (BK008; Cytoskeleton, Inc) was used to assess KRAS activity following manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... the microtubules were labeled by SiR700-tubulin Kit (Cytoskeleton) at a final concentration of 800 nM in cell culture medium for 2 h before imaging.
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Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavHbioRxiv - Molecular Biology 2023Quote: ... Actin polymerization biochem kit (BK003) was purchased from Cytoskeleton Inc ...
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bioRxiv - Cancer Biology 2022Quote: ... the RAS-GTP pull-down assay kit (Cytoskeleton #BK008) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... G-LISA RhoA or Rac1 activation kits from Cytoskeleton were used according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... the samples were washed 2× with IF buffer and incubated with rhodamine phalloidin (PHDR1) (1:500) (Cytoskeleton, Inc.) for 30 minutes in the dark at 4 °C ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were then stimulated with vehicle or 100ng/ml EGF for 15 minutes or 2 hours and lysed with Cell Lysis Buffer (50mM Tris pH 7.5, 10mM MgCl2, 0.5M NaCl, 2% Igepal) (Cytoskeleton Inc.) containing protease and phoshatase inhibitors (Pierce/Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... 2% BSA) prior to immunostaining for 20 min with a 1:500 dilution of Acti-stain-555 (Cytoskeleton Inc). The ratio of cytoplasmic actin to cortical actin was measured using ImageJ (Fiji 2.1.0/1.53c ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed 2 times with 1xPBS and then incubated with rhodamine phalloidin (PHDR1; 1:500) (Cytoskeleton Inc.) in IF wash buffer for 30 min at RT in the dark ...
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bioRxiv - Microbiology 2022Quote: Proteins were collected in cell lysis buffer (50mM Tris pH 7.5, 10mM MgCl2, 0.5M NaCl, 2% Igepal) supplemented with 1X Protease inhibitor cocktail (Cytoskeleton, PIC02). Lysates were cleared by 1min centrifugation at 10,000g at 4°C and the collected supernatants were snap-frozen in liquid nitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... phenol-red free DMEM and incubated with MemGlow dyes (MemGlow 560, MG02-2 and MemGlow 590, MG03-10; Cytoskeleton) in serum-free ...
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bioRxiv - Cell Biology 2020Quote: ... the Cd42 G-LISA kit (Cytoskeleton Inc., Denver, CO, USA) was used according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... For ubiquitin pulldown assays using the SignalSeeker kit (Cytoskeleton Inc), cells were lysed in provided BlastR buffer with protease inhibitor and NEM ...