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Citations for Cytoskeleton :
101 - 150 of 181 citations for QuantiChrom Indican Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... was measured using Advanced Protein Assay Reagent (Cytoskeleton, Inc.) with bovine serum albumin as a standard ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc.) and 50 μg total protein were separated on SDS-PAGE followed by western blot ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was measured with Precision Red Assay (Cytoskeleton). Laemmli sample buffer (Biorad ...
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bioRxiv - Genetics 2024Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc.) and 50 - 100 μg total protein was separated on SDS-PAGE followed by western blot ...
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bioRxiv - Cell Biology 2020Quote: ... 3 G-LISA™ Activation Assay (purchased from Cytoskeleton Inc.), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... the colorimetric-based RAS G-LISA activation assay (Cytoskeleton #BK131) was used ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein quantification was performed using Precision Red Protein Assay Reagent (Cytoskeleton) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentration was determined using Precision Red Advanced Protein Assay (Cytoskeleton, Inc.). 20ug protein were loaded onto 8-12% SDS-PAGE gels and transferred onto nitrocellulose membranes ...
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bioRxiv - Cell Biology 2020Quote: Colchicine-competition assays were performed with 2 μM purified tubulin (Cytoskeleton, Inc) taken from a 10 mg/mL stock prepared in tubulin buffer as above ...
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bioRxiv - Genetics 2024Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc. Denver, CO, USA) and 50 μg total protein were separated on SDS-PAGE followed by western blot ...
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bioRxiv - Cancer Biology 2021Quote: The microtubule polymerisation assay was performed using porcine neuronal tubulin (Cytoskeleton, Inc, BK006P) as an adaptation of the original method of Shelanski et al ...
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bioRxiv - Molecular Biology 2021Quote: Rho-GTPase pull-down assays were performed following the manufacturer’s protocol (Cytoskeleton, Inc.). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... The dGAE protein concentration was measured using Advanced Protein Assay Reagent (Cytoskeleton, Inc.) with bovine serum albumin as a standard ...
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bioRxiv - Microbiology 2022Quote: Rac activation was measured with a Rac123-G-LISA assay (absorbance based; Cytoskeleton) and performed according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Microtubule polymerization assays were performed using 1.5 mg/mL purified tubulin (Cytoskeleton, Inc) that was taken from a 10 mg/mL flash frozen stock ...
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bioRxiv - Biochemistry 2019Quote: ... The final assay solution contained pM concentrations of 10 - 15% biotinylated actin (Cytoskeleton Inc,) from rabbit skeletal muscle stabilized by rhodamine phalloidin ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined using the Precision Red Advanced Protein Assay Reagent (Cytoskeleton ADV02). Extracted proteins (25-35 μg ...
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bioRxiv - Cell Biology 2019Quote: ... and protein concentration determined using the Precision Red™ Advanced Protein Assay Reagent (Cytoskeleton). Respective samples were treated with 300nM Nocodazole for 15 min before lysis ...
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bioRxiv - Cancer Biology 2023Quote: Rac1 activity assay was performed according to the manufacturer’s protocol (Cat# BK035, Cytoskeleton Inc). DBTRG or HF2303 cells were transfected with Rac1-WT ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: In vitro tubulin polymerization assays were performed according to the manufacturer’s instructions (Cytoskeleton, Inc.) optimized for polymerization inhibitors ...
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bioRxiv - Neuroscience 2020Quote: ... and protein concentration estimated in the supernatant using Precision Red Advanced Protein Assay (Cytoskeleton, CA). Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad) ...
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bioRxiv - Developmental Biology 2020Quote: ... total cellular active RhoA levels were measured using the Rho Activity Assay (Cytoskeleton Inc., BK036) following the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2022Quote: The Microtubule Binding Protein Spin-down assay was performed according to the manufacturers’ protocol (Cytoskeleton). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were then used for G-LISA small GTPase activation assay (Cytoskeleton cat# BK135), where levels of activated RhoA ...
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bioRxiv - Immunology 2023Quote: ... Protein concentrations of crude extracts were determined using the Advanced Protein Assay (Cytoskeleton, Denver, USA).
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bioRxiv - Cell Biology 2023Quote: ... Polymerization assays were performed using a mixture of commercial rhodamine labeled actin monomers (Cytoskeleton, Inc.) and unlabeled actin monomers that were mixed at a 1:1 ratio ...
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bioRxiv - Cell Biology 2019Quote: ... and protein concentrations were measured using the Precision Red Advanced Protein Assay Reagent (# ADV02; Cytoskeleton Inc.) and finally equalized with ice-cold lysis buffer to 1.0 mg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... Different types of labelled and unlabeled purified tubulin used in the assays were either purchased from Cytoskeleton or purified as described previously (57 ...
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bioRxiv - Molecular Biology 2022Quote: ... Active RhoA was detected in a colorimetric RhoA activation assay (G-LISA) (BK124, Cytoskeleton, Denver, CO, USA).
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration of the cleared lysates was determined by Precision Red Advanced Protein Assay Reagent (Cytoskeleton Inc.). Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: Rac1 activation was measured by means of a G-LISA Activation Assay with luminescence read-out (Cytoskeleton, Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Actin Polymerization Biochem Kit (Cytoskeleton) was used to investigate F-actin depolymerization by cofilin in the absence or presence of control protein BSA ...
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bioRxiv - Neuroscience 2021Quote: Effects of tau and nocodazole on MT polymerization were tested using a Tubulin Polymerization Assay (Cytoskeleton Inc., Denver, CO). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... provided along with the kit (Cytoskeleton) was used to detect G- and F-actins ...
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bioRxiv - Molecular Biology 2021Quote: ... the pathway was activated with PDGF-AA at 50 ng/ml for 20 minutes at 37°C and the G-LISA assay (Cytoskeleton) was performed following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: The co-sedimentation assay was adapted from the in vitro polymerization assay from (Venkei et al. 2006) as follows: 60 μM porcine brain tubulin (Cytoskeleton) was polymerised into microtubules in BRB80 buffer (1x ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... Whole colonic tissue was homogenized in cell lysis buffer and protein concentration was determined using the Precision Red Protein Assay (Cytoskeleton). Samples were sent to Eve Technologies for analysis using their 32-multiplex Mouse Cytokine/Chemokine Array.
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bioRxiv - Developmental Biology 2021Quote: ... cleaved proteins (quantified via a Bradford Assay) were incubated at room temperature for one hour with 0 or 5μM polymerized chicken F-actin (Cytoskeleton, Inc.). BSA was used a negative control ...
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bioRxiv - Cell Biology 2022Quote: Guanine nucleotide exchange (GEF) activity of ERK3 (9-327 aa) was further evaluated using RhoGEF Exchange Assay (Cat# BK100, Cytoskeleton). Assay was performed according to the manufactures’s instructions and included Dbs GEF as a positive control.
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bioRxiv - Neuroscience 2023Quote: ... the cells were lysed in 250 µl cell lysis buffer supplemented with protease inhibitors and subjected to a pull-down assay for activated Rac1 (#BK035, Cytoskeleton). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected upon 15min of centrifugation at >15000 rpm at 4°C and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). Equal amounts of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... GAP assays were performed as described previously (Faix et al., 1998) except that GTP hydrolysis was quantitated using CytoPhos reagent (Cytoskeleton Inc) to measure the amount of free phosphate ...
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bioRxiv - Neuroscience 2021Quote: Hippocampal and cortical tissue from WT and HTZ mice were lysed and homogenized in conditions that stabilize filamentous (F-) and monomeric (G-) actin using an F/G actin commercial assay (Cytoskeleton Inc.). Lysed extracts were ultracentrifuged at 100,000 g for 1 h at 37 °C in order to separate pellets (F-actin ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: Dynamic MT seed assays were performed by polymerizing GMPCPP stabilized Cy5-MT seeds from 4 µM HiLyte 647 tubulin (Cytoskeleton, #TL670M) in BD buffer + 0.5 mM GMPCPP at 37°C for 1 hour ...
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bioRxiv - Microbiology 2019Quote: ... 5 μL of the filtered supernatant was pipetted into a clear flat-bottom UV plate and 250 μL of 1x Advanced Protein Assay Reagent (Cytoskeleton Inc., CO) was added and mixed ...
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bioRxiv - Cell Biology 2020Quote: Activated RhoA pulldown kits were purchased from Cytoskeleton. Sample lysates were prepared similar to the protocol used for G-LISAs ...
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bioRxiv - Physiology 2022Quote: ... the Cdc42 activation kit (Cytoskeleton Inc., Denver, USA) was used according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Bound activated RhoA was eluted from the beads and analyzed by western blotting using a RhoA antibody.To measure the levels of active Rac1 and Cdc42 were measured using the Rac1 and CDC42 Activity Assay (Cytoskeleton Inc., BK035 and BK034, respectively), cells were lysed in a buffer containing 5 mM DTT ...
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bioRxiv - Cell Biology 2020Quote: Commercial ELISA kits were purchased from Cytoskeleton (G-LISA) to quantify RhoA and Rac1 activation (Cytoskeleton) ...