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Citations for Cytoskeleton :
51 - 100 of 123 citations for Poly rC Binding Protein 4 PCBP4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... was measured using Advanced Protein Assay Reagent (Cytoskeleton, Inc.) with bovine serum albumin as a standard ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc.) and 50 μg total protein were separated on SDS-PAGE followed by western blot ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was measured with Precision Red Assay (Cytoskeleton). Laemmli sample buffer (Biorad ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein was quantified using the Precision Red (#ADV02; Cytoskeleton) advanced protein assay and 10-20μg was used ...
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bioRxiv - Genetics 2024Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc.) and 50 - 100 μg total protein was separated on SDS-PAGE followed by western blot ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were fixed in PFA (4% PFA in Cytoskeleton Buffer (CB) – in mM ...
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bioRxiv - Plant Biology 2019Quote: ... The purified Arp2/3 protein complex from porcine brain (Cytoskeleton) and GST-tagged VCA domain of human WASP protein (Cytoskeleton ...
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bioRxiv - Plant Biology 2019Quote: ... and GST-tagged VCA domain of human WASP protein (Cytoskeleton) were used to initiate actin nucleation ...
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bioRxiv - Cell Biology 2023Quote: ... and total protein concentration was measured using Precision Red (Cytoskeleton). Normalized samples were denatured with SDS loading buffer (250mM Tris [pH 6.8] ...
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bioRxiv - Cell Biology 2023Quote: The Rhotekin-RBD Beads (Binds Active Rho Proteins) from Cytoskeleton Inc ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were incubated in primary antibody diluted in TBST (1:500 anti-RHOA antibody, Cytoskeleton ARH04) or diluted in block (1:30,000 anti-a-alpha tubulin antibody ...
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bioRxiv - Neuroscience 2023Quote: ... then rinsed and sequentially incubated with primary antibodies to RhoA followed by HRP secondary antibodies (Cytoskeleton), and the colorimetric reaction was performed for 15 min at 37 °C ...
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bioRxiv - Biophysics 2021Quote: The following cytoskeleton proteins are purchased: porcine brain tubulin (Cytoskeleton T240), HiLyte Fluor-488-labeled tubulin (Cytoskeleton TL488M-A) ...
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bioRxiv - Cell Biology 2020Quote: ... reserving a small amount for protein quantification using Precision Red (Cytoskeleton). Prior to measuring GTPase activity ...
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bioRxiv - Cell Biology 2021Quote: Soluble tubulin polymerization mix containing 50 μM tubulin protein (Cytoskeleton, T240), 1 μM TRITC-labeled tubulin protein (Cytoskeleton ...
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bioRxiv - Cell Biology 2021Quote: ... 22 μl of 5 mg ml−1 tubulin protein (Cytoskeleton, T240) was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton ...
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bioRxiv - Microbiology 2024Quote: Microtubules were made by mixing 4 μg of HiLyte Fluor 488 tubulin (Cytoskeleton), 4 μg of biotinylated tubulin (Cytoskeleton ...
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bioRxiv - Genetics 2024Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc. Denver, CO, USA) and 50 μg total protein were separated on SDS-PAGE followed by western blot ...
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bioRxiv - Biochemistry 2023Quote: ... and protein concentrations were estimated using the Precision Red reagent (Cytoskeleton Inc). Protein samples were prepared by mixing 1X LDS (Invitrogen) ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: MTs were polymerized by incubating 5 µM tubulin (4% HyLite 647 tubulin (Cytoskeleton #TL670M), ∼1% biotin tubulin (Cytoskeleton #T333P) ...
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bioRxiv - Biophysics 2021Quote: ... the buffer also contained 50 µM non-muscle human actin protein (Cytoskeleton, Inc) and 0.2 mM ATP and 0.2 mM CaCl2.
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bioRxiv - Cell Biology 2021Quote: ... A mix of 20 μM of porcine brain tubulin protein (Cytoskeleton/Tebu-Bio) with 1 mM GMPCPP (Jena Bioscience ...
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bioRxiv - Biophysics 2020Quote: 0.4 µM Myosin Motor protein S1 Fragment (Rabbit Skeletal Muscle, Cytoskeleton #CS-MYS04) was incubated either alone or with 2 µM UCS proteins for 10 minutes either at 42°C or on ice in Mg-buffer (10mM Hepes-KOH ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... and Tyr-phosphorylated proteins were collected using Anti-phosphotyrosine Affinity Beads (Cytoskeleton, USA). Proteins were concentrated using the TCA/acetone precipitation method (Fic et al. ...
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Gα13 loss promotes tumor progression in the KPC transgenic mouse model of advanced pancreatic cancerbioRxiv - Cancer Biology 2021Quote: ... and the protein concentration was determined using Precision Red solution (Cytoskeleton, Inc., Denver, CO) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... and the protein concentration was determined using Precision Red solution (Cytoskeleton, Inc., Denver, CO). Equal amounts of protein were separated with a 10% SDS-PAGE electrophoresis gel ...
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal Mouse anti-RhoA antibody (Cytoskeleton inc. Cat#ARH05) was used at 1:500 dilution in TBST overnight.
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal Mouse anti-RhoA antibody (Cytoskeleton inc. Cat#ARH05) was obtained through the Cytoskeleton inc Rho A activation Assay Biochem Kit and used at 1:500 ...
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bioRxiv - Immunology 2022Quote: ... Monoclonal Rac1 antibodies (Cat. # ARC03) were purchased from Cytoskeleton.
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bioRxiv - Immunology 2022Quote: Monoclonal Rac1 antibodies (Cat. # ARC03) were purchased from Cytoskeleton.
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bioRxiv - Cell Biology 2020Quote: In order to constitutively activate RhoA vessels at day 4 were perfused with Rho Activator II (Cytoskeleton) at a concentration of 2 µg/mL for four hours prior to use in permeability tests or fixation for immunocytochemistry ...
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bioRxiv - Microbiology 2020Quote: ... Samples were fixed in 4% (v/v) Paraformaldehyde Aqueous Solution (15710-s, EM Grade) in PEM (Cytoskeleton-preserving buffer PIPES-EGTA-Magnesium ...
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bioRxiv - Plant Biology 2020Quote: ... 13 nM Arp2/3 protein complex and 15 nMWASp-VCA domain-GST purified (Cytoskeleton, USA) along with freshly purified ArF-BAR protein in concentrations of 200 ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal protein levels were confirmed by a control western blot of rabbit anti-Kinesin (Cytoskeleton) from the cleared lysates ...
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bioRxiv - Cell Biology 2022Quote: ... Pyrene-labeled actin was incubated with recombinant ARP2/3 protein complex alone (Cat# RP01P, Cytoskeleton) or along with the human recombinant proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolation of ubiquitinated proteins was performed with the Signal-Seeker Ubiquitin Enrichment kit (Cytoskeleton). Cell fractionation was performed with the Qproteome Cell Compartment kit (Qiagen).
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bioRxiv - Molecular Biology 2019Quote: Mouse antibodies against Myc-tag (M185-3L/M047-3, MBL, Nagoya, Japan), rabbit antibody against GFP-tag (D110008, BBI, Shanghai, China) and sheep antibody against Tubulin (ATN02, Cytoskeleton, Denver, United States) were subjected to Western blot according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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bioRxiv - Neuroscience 2023Quote: ... or Cdc42 activation in vivo assay biochemical kits for the respective protein (Cytoskeleton Inc., Denver, CO). The tissues were lysed in lysis buffer (50mM Tris-HCl pH7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred to a clean tube and quantified using PrecisionRed (Cytoskeleton; ADV02-A) at OD600.
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bioRxiv - Biochemistry 2024Quote: ... The cells were lysed and protein lysates were used for G-LISA analysis per manufacturer’s (Cytoskeleton) instructions ...
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bioRxiv - Plant Biology 2020Quote: The actin polymerization modulation activity of the proteins was checked using Actin Polymerization Biochem Kit (Cytoskeleton, USA), using the manufacturer’s instruction ...
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bioRxiv - Biophysics 2024Quote: ... GST-tagged WCA domain of human WASP protein (GST-WCA) was purchased from Cytoskeleton (Cat. #VCG03-A) and used without further purification ...
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bioRxiv - Cell Biology 2020Quote: Primary antibodies used were rabbit anti-actin polyclonal (Cytoskeleton, Denver, CO) and mouse anti–MYO-3 monoclonal (5–6 ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with a polyclonal antibody against actin (1:500; Cytoskeleton Inc.) and developed by chemiluminescence ...
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal mouse anti-Cdc42 monoclonal antibody (1:250, Cytoskeleton, Cat. # ACD03), anti-Rac1 (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... actomyosin contractility of MECs stably expressing an inducible ROCK construct was induced overnight using 4-hydroxytamoxifen and the actin cortex of these cells was labelled with SiR-actin dye (Cytoskeleton) according to manufacturer’s instructions prior to laser ablation.
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bioRxiv - Cell Biology 2021Quote: ... and incubation in secondary antibody and Acti-Stain 670 Phalloidin (Cytoskeleton, inc), plus Hoechst (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... moderate amounts of anti-ac-K antibody agarose beads (Cytoskeleton, Inc., USA) were added to the lysate and incubated for 12 h at 4°C ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: Dynamic MT seed assays were performed by polymerizing GMPCPP stabilized Cy5-MT seeds from 4 µM HiLyte 647 tubulin (Cytoskeleton, #TL670M) in BD buffer + 0.5 mM GMPCPP at 37°C for 1 hour ...