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Citations for Cytoskeleton :
1 - 50 of 68 citations for Phosphatase Inhibitor Cocktail 2 Tubes 100X since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... and protease inhibitor cocktail (Cytoskeleton). Equal amounts of protein were subject to SDS-PAGE and blotted with polyclonal anti-phosphorylated ROCK2 (Ser1366 ...
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bioRxiv - Cell Biology 2022Quote: ... with 1x Protease inhibitor cocktail (PIC02, Cytoskeleton) and 1x De-Ubiquitination/ SUMOylation inhibitor (N-ethylmaleimide and TPEN ...
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bioRxiv - Cell Biology 2020Quote: ... containing protease inhibitor cocktail (Cytoskeleton, Inc., Denver, CO), and cleared by centrifugation (20,000 x g) ...
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bioRxiv - Microbiology 2022Quote: Proteins were collected in cell lysis buffer (50mM Tris pH 7.5, 10mM MgCl2, 0.5M NaCl, 2% Igepal) supplemented with 1X Protease inhibitor cocktail (Cytoskeleton, PIC02). Lysates were cleared by 1min centrifugation at 10,000g at 4°C and the collected supernatants were snap-frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2019Quote: ... β-glycerophosphate (5μM) and 1x Protease Inhibitor Cocktail (Cytoskeleton). Total protein concentration was determined using BCA Protein Assay Kit (Pierce) ...
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bioRxiv - Microbiology 2019Quote: ... β-glycerophosphate (5μM) and 1x Protease Inhibitor Cocktail (Cytoskeleton). The protein concentration of each sample was determined using BCA Protein Assay Kit (Pierce) ...
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bioRxiv - Cancer Biology 2020Quote: ... or 2 μg/mL Rho A Inhibitor I (Cytoskeleton, Inc.) conditions and imaged immediately after designated incubation time as per commercial protocol (Cytoskeleton Inc.) ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... Rho inhibitor I (Cytoskeleton, CT04-A, used at 2 μg/ml), Y-27632 (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... The treatment consisted of 2 µg/ml of Rho inhibitor I (Cytoskeleton, CT04) for 2 h or 20 µM of Y27632 (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... Rho Inhibitor I (Cytoskeleton) was used at a final concentration of 0.5ug/ml for 6 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... the Rho inhibitor CT04 (Cytoskeleton Inc.), and the Rho activator CN03 (Cytoskeleton Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... Rho inhibitor: C3 exoenzyme (Cytoskeleton, CT04); ROCK inhibitor ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μl biotinylated tubulin (2 mg/ml, Cytoskeleton) and 7 μl unlabelled pig tubulin (Schlager et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... and De-ubiquitination/sumoylation Inhibitor (Cytoskeleton NEM09BB) was incubated with Anti-Flag M2 Affinity Gel or Ubiquitination Affinity Beads (UAB ...
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bioRxiv - Cell Biology 2021Quote: ... or Rho inhibitor I (CT04, Cytoskeleton, Inc.) were added to the plating medium 30 h after plating.
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bioRxiv - Cancer Biology 2023Quote: ... The Rho inhibitor (Cytoskeleton, Inc, Cat# CT04) was administered at a concentration of 2μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... and Rho Inhibitor I (Cytoskeleton, Cat# CT04) were utilized on the same day after dilution ...
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bioRxiv - Cell Biology 2020Quote: ... for 2 h or with CT04 (2 μg/ml, Cytoskeleton) for 3 h ...
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bioRxiv - Cell Biology 2020Quote: ... vessels were perfused with Rho Inhibitor I (Cytoskeleton) at a concentration of 2 µg/mL for four hours prior to use in permeability tests or immunocytochemistry.
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bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred to a clean tube and quantified using PrecisionRed (Cytoskeleton; ADV02-A) at OD600.
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bioRxiv - Cell Biology 2019Quote: ... and Rho Inhibitor I C3 (Cytoskeleton, 0.5 µg/ml).
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bioRxiv - Molecular Biology 2023Quote: ... C3 (Rho inhibitor I, cat # CT04) was from Cytoskeleton. Anti-β-actin (cat # 4970) ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1 μg/ml RhoA inhibitor-I (Cytoskeleton, Inc., #CT-04), 2 μM FK-506 (Cayman No ...
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bioRxiv - Cancer Biology 2023Quote: ... Rho inhibitor I (C3 transferase) was purchased from Cytoskeleton (#CT04). YM-254890 was purchased from FUJIFILM Wako Pure Chemical Corporation (#257-00631).
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bioRxiv - Neuroscience 2019Quote: ... 1.0 µg/mL of RhoA inhibitor C3 transferase (Cytoskeleton, Cat# CT04) for 16 hours ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μM unlabeled porcine tubulin (Cytoskeleton) prepared in binding buffer containing 10 mM DTT and 0.2 mM PMSF was added and incubated overnight on a shaker in a cold room ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell-permeable C3 transferase-based Rho inhibitor 1 was from Cytoskeleton. Saracatinib and defactinib were from Selleck Chemicals ...
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bioRxiv - Cell Biology 2022Quote: ... The following inhibitors were used: C3 exoenzyme (1 mg/ml; Cytoskeleton, Inc), NSC23766 (50 mM ...
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bioRxiv - Cell Biology 2022Quote: ... and 1x De-Ubiquitination/ SUMOylation inhibitor (N-ethylmaleimide and TPEN, NEM09BB, Cytoskeleton)) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were stored in G-LISA lysis buffer with manufacturer’s protease inhibitors (Cytoskeleton). Protein concentrations were determined using Precision Red Advanced Protein Assay Reagent with absorbance at 600 nm measured spectrophotometer ...
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bioRxiv - Cell Biology 2022Quote: ... CT04 (Cytoskeleton; 2 µg/mL; vehicle: dH2O) was added to the media for 2 hours as pre-treatment plus an additional 4 hours with or without Wnt7a.
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bioRxiv - Neuroscience 2023Quote: ... Y27632 from Calbiochem (Cat Number: 688000) and C3 toxin (Rho Inhibitor I) from Cytoskeleton (Cat Number: CT04).
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bioRxiv - Microbiology 2023Quote: ... IP of total SUMOylated protein fractions used either SUMO-1 or SUMO-2/3 Ab included with the Signal Seeker SUMOylation 1 or 2/3 Detection Kit (Cytoskeleton) and IPs were carried out according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2‰ MemGlow 560 (Fluorogenic Membrane Probe, Cytoskeleton), and imaged on the imaging setup described below.
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bioRxiv - Cell Biology 2022Quote: ... 2 µL of 10 mg/mL unlabeled tubulin (Cytoskeleton) was mixed with 2 µL o f 1 mg/mL biotin-tubulin and 2 µL of 1 mg/mL dye-labeled tubulin on ice ...
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bioRxiv - Biophysics 2023Quote: ... we performed additional tests: incubation with 1.0 μg/ml C3-transferase (RhoA inhibitor, Cytoskeleton, cat. CT04) and with 1.0 μg/ml of RhoA activator (Cytoskeleton ...
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bioRxiv - Evolutionary Biology 2023Quote: ... testes were stained with 2 μM SiR-Actin59 (Cytoskeleton, Inc.) for 2-3 hours at room temperature and incubated with Hoechst 33342 (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... 2 μL of 1 mg/mL biotinylated tubulin (Cytoskeleton Inc., #T333P), and 2 μL of 10 mg/mL unlabeled tubulin (Cytoskeleton Inc. ...
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bioRxiv - Cell Biology 2021Quote: ... WT Caco-2 cells were treated with 1ug/ml CN03 (Cytoskeleton, Inc) or 1μg/ml C3 Transferase (Cytoskeleton ...
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bioRxiv - Biophysics 2020Quote: ... Biotin-labeled actin (2 µM) was prepared using 10% biotin actin (Cytoskeleton) in KMg25 buffer ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 μL of 10 mg/mL unlabeled tubulin (Cytoskeleton Inc., #T240) was mixed on ice ...
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bioRxiv - Cell Biology 2020Quote: Colchicine-competition assays were performed with 2 μM purified tubulin (Cytoskeleton, Inc) taken from a 10 mg/mL stock prepared in tubulin buffer as above ...
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bioRxiv - Cell Biology 2022Quote: ... the Rho Inhibitor I ADP ribosylation of Rho Asn-41 (CT04) and Rho Activator II (CN03) were obtained from Cytoskeleton. The phosphor-ezrin inhibitor NSC668394 was from Calbiochem ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 1xPBS and lysed in appropriate volume of lysis and F-actin stabilization buffer supplemented with 1mM ATP and protease inhibitor mixture for F-actin stabilization (Cytoskeleton). Lysates were centrifuged at 100 000 x g at 37°C for 1 h to separate the G-actin fraction in the supernatants and pelleted F- actin ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were lysed in 250 µl cell lysis buffer supplemented with protease inhibitors and subjected to a pull-down assay for activated Rac1 (#BK035, Cytoskeleton). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... Rho Inhibitor I (C3 Transferase from Clostridium botulinum covalently linked to a cell penetrating moiety; 10μM; Cytoskeleton, Inc #CT04-A); Rac GTPase ...
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bioRxiv - Biophysics 2021Quote: Cells were incubated with 1-2 μM of SiR-Actin (Cytoskeleton, Inc. Spirochrome) for 15-30 min in calcium-free buffer ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated 2 hours with C3-transferase (Cytoskeleton Inc., cat. CT03-A) at a concentration of 1μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... The exoenzyme C3 transferase (Rho inhibitor I) and the Rho activator II (CN03) were purchased from Cytoskeleton (#CT04 and #CN03, respectively). Coelenterazine 400a was purchased from NanoLight Technology (#340).
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bioRxiv - Cell Biology 2019Quote: ... 2 μM rabbit skeletal muscle actin (90% unlabeled and 10% rhodomine-actin) (Cytoskeleton Inc.), 1 μM Shi were incubated in 6.5 mM HEPES ...