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Citations for Cytoskeleton :
151 - 200 of 231 citations for Mouse T Cell Surface Glycoprotein CD8 Beta Chain CD8B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: Actin pelleting experiments were performed using the G-Actin/F-actin In Vivo Assay Biochem Kit (Cytoskeleton, Inc.) with modifications ...
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bioRxiv - Cell Biology 2021Quote: Cdc42 and Rac1 Pull-Down assays were carried out using Cdc42/Rac1 Activation Assay Biochem Kits (Cytoskeleton, inc), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Actin bundling assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem Kit (Cytoskeleton Inc.) according to protocol of the manufacturer.
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bioRxiv - Cell Biology 2024Quote: ... Rho-GTP was measured using the RhoA G-LISA Activation Assay Kit (Colorimetric Format; Cat# BK124) from Cytoskeleton, according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... cell lysate (125 μg) was added to 5 μg GGA3-PBD beads (Cytoskeleton, USA) to pull down ARFs-GTP and incubated at 4°C on a rotator for 1h and then the beads were extensively washed to remove unbound protein ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed with PBS and treated with SiR-DNA dye (CY-SC007, Cytoskeleton) at 300 nM for 6 hours before starting imaging.
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bioRxiv - Neuroscience 2020Quote: ... cells were also further incubated for 30 min with Alexa-670-phalloidin (Cytoskeleton #PHDN1) to label for actin filaments ...
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bioRxiv - Microbiology 2022Quote: ... around 3× 107 trophozoites were incubated in cell lysis buffer (Cytoskeleton Inc, Colorado, USA) with vigorous agitation at 4°C for 30 min and homogenized by a 23-gauge needle on a 5-ml syringe ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated in culture media containing SiR-Tubulin (Spirochrome/Cytoskeleton Inc., Denver, CO). Single cell suspensions were generated as above ...
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bioRxiv - Cancer Biology 2021Quote: ... the Rac1- and cdc42 Small GTPase Activation Assay kits (G-LISA) were used (027BK127-S, 027BK128-S, Cytoskeleton, Inc) following the company’s guidelines.
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bioRxiv - Cell Biology 2020Quote: ... and G-LISA assay was performed as per the manufacturer’s instructions (Rac1 and Cdc42 G-LISA activation assay kits, Cytoskeleton).
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bioRxiv - Cell Biology 2021Quote: The pyrene-actin polymerization and depolymerization assays were performed using the Actin Polymerization Biochem Kit (Cytoskeleton, Inc., Denver, CO) following the manufacturer protocol ...
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bioRxiv - Systems Biology 2022Quote: The Rho activity was measured and quantified by using the RhoA Activation Assay Biochem Kit (cat. no. BK036; Cytoskeleton) based on the Rhotekin pull-down assay as per the manufacturer’s instructions as described previously(39) ...
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bioRxiv - Biochemistry 2022Quote: ... colorimetric Rac1 and RhoA G-LISA Activation Assay Kits (BK128-S and BK124-S Cytoskeleton Inc., Denver CO, USA) were used according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2021Quote: F-actin disruption assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem kit (Cytoskeleton, USA) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Actin protein levels were calculated using a G-actin/F-actin in vivo Assay Biochem Kit as per manufacturer’s instructions (Cytoskeleton). Briefly ...
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bioRxiv - Immunology 2021Quote: ... GTP-bound form of CDC42 was pulled down for analysis using the Cdc42 Pull-Down Activation Assay Kit (Cytoskeleton). FLAG-tagged protein pull-down assays were performed using DDDDK tagged protein purification kit (MBL) ...
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bioRxiv - Cancer Biology 2023Quote: GTP-bound RAS levels were assessed with a RAS G-LISA Assay Kit per manufacturer’s instructions (Cytoskeleton INC., #BK131). 9 ξ 105 cells were plated in a 3D 24-well plate ...
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bioRxiv - Cell Biology 2020Quote: For the determination of active GTP bound form of Cdc42 we used the G-LISA kit for Cdc42 from Cytoskeleton according to the protocol of the manufacturer.
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bioRxiv - Biophysics 2020Quote: ... Rac1-GTP and Cdc42-GTP using the RhoA and Cdc42 pull-down activation assay biochem kit (PN:BK036-S and BK034-S, Cytoskeleton, Inc.) following the protocol provided by the manufacturer ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: Globular and filamentous actin ratios were determined by western blot as described by commercially available G-actin/ F-actin In Vivo Assay Kit (Cytoskeleton). Globular and filamentous immunocytochemistry was performed as previously described [53] ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysate preparation and measurement of RHOA-GTP levels were carried out with the RHOA G-LISA activation assay kit (#BK124, Cytoskeleton) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: G- and F-actin were fractionated and enriched using a commercial in vivo assay biochem kit (Cytoskeleton Inc, Colorado, USA), according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Molecular Biology 2020Quote: ... Intracellular Rac1 activity was determined using a Rac1 G-LISA Activation Assay Kit (Colorimetric format) (Cytoskeleton, catalog BK128, Denver, CO) using 25μg total protein per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Intracellular RhoA activity was determined using a RhoA G-LISA Activation Assay Kit (Colorimetric format) (Cytoskeleton, catalog BK124, Denver, CO) using 20μg total protein per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... In vitro microtubule-binding assay was performed using the microtubule-binding protein spin down assay kit (Cytoskeleton, Denver, CO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: The protocol of G and F actin measurement mostly followed the instructions of the “G-actin /F-actin In Vivo Assay Kit” from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2022Quote: ... and Rac or Rho activity was assessed in duplicate using the G-LISA small G-protein activation assay kit (BK128, BK124, Cytoskeleton) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... IP of total SUMOylated protein fractions used either SUMO-1 or SUMO-2/3 Ab included with the Signal Seeker SUMOylation 1 or 2/3 Detection Kit (Cytoskeleton) and IPs were carried out according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: RhoAGTP and Rac1GTP pull down as-says were performed using the RhoA and Rac1 Activation Assay Biochem Kit (Cytoskeleton BK030) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... cells were fixed and stained with Acti-stain 670 phalloidin (Cytoskeleton #PHDN1-A, 70 nM) and DAPI as for immunofluorescence experiments ...
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bioRxiv - Biophysics 2021Quote: ... Washed cells were diluted 1:9 and stained with 50 nM SiR-actin (Spirochrome/Cytoskeleton) for 30 minutes (Lukinavicius et al. ...
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bioRxiv - Cell Biology 2020Quote: ... the cell culture media was replaced with CO2-independent media containing 100μM SiR-tubulin (Cytoskeleton). For conditions involving UMK57 (a kind gift from Duane Compton ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were treated with 75 nM Hoescht 33342 and 200 nM SiR-tubulin (Cytoskeleton Inc.) for 90 minutes prior to imaging ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then stained with 14 μM Acti-stain 555 phalloidin (Cytoskeleton; cat#PHDH1-A) at room temperature in the dark for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were then used for G-LISA small GTPase activation assay (Cytoskeleton cat# BK135), where levels of activated RhoA ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with a 1:1000 dilution of SPY555-Actin Probe (CY-SC202, Cytoskeleton) in complete DMEM at 37°C for three hours prior to imaging ...
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bioRxiv - Bioengineering 2023Quote: ... with cell morphology and nucleus visualized using Acti-stain 555 phalloidin (1:2000, Cytoskeleton, PHDH1A) and DAPI (2ng/ml ...
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bioRxiv - Cell Biology 2024Quote: ... the cilia of cultured multiciliated cells were live-labeled using SiR-tubulin (Cytoskeleton, #CY-SC002) or Spy555-tubulin (Cytoskeleton ...
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bioRxiv - Immunology 2021Quote: Activation of ARF6 (ARF6-GTP) was quantitated using the Arf6 Pull-Down Activation Assay Biochem Kit from Cytoskeleton (BK033-S, USA) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: G/F-actin assay was performed according to the manufacturer’s instructions (G/F-actin In Vivo Assay Kit, Cytoskeleton, Denver, CO). G-and F-actin bands on western blots were scanned by densitometry and the ratios of free G-actin to F-actin were calculated.
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bioRxiv - Microbiology 2022Quote: ... 110 μl of LAS2 buffer was prepared according to the protocol of a G-actin/F-actin In Vivo Assay Biochem Kit (Cytoskeleton, Inc.) and mixed with the pellet ...
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bioRxiv - Cell Biology 2023Quote: The F-actin/G-actin ratio was measured using G-Actin/F-Actin In Vivo Assay Biochem Kit (#BK037, Cytoskeleton, Inc.), following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: F- and G-actin fractions from lysates were prepared using a G-Actin/F-Actin in Vivo Assay Biochem kit (Cytoskeleton, #BK037) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Western blots to determine the ratio of G-actin to F-actin were performed on retinal lysates that were processed using a G-actin/F-actin In Vivo Assay Kit (Cytoskeleton, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... NIH3T3 cells were imaged using four wavelengths (405 nm for tagBFP; 488 nm for Citrine; 561 nm for mCherry or FusionRed; 674 nm for SiR700-Tubulin Kit, #CY-SC014, Cytoskeleton Inc.).
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bioRxiv - Neuroscience 2024Quote: Active GTP-bound GTPase levels in brain lysates were measured using G-LISA activation assay kits for Rac1 (Cytoskeleton, Inc., BK128) and RhoA (Cytoskeleton ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained with 1 µM SiR-actin and 10 µM verapamil (Cytoskeleton, Inc., CY-SC001) for 1-3 hours before imaging ...
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bioRxiv - Microbiology 2020Quote: ... The cells were washed twice with PBS and incubated with rhodamine phalloidin (1:500) (Cytoskeleton Inc.) in IF washing buffer for 30 minutes at room temperature in the dark ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated in fresh hormone depletion media supplemented with 1µM Sir-Hoechst (Cytoskeleton, Cy-SC007). Cells were placed on the microscope in hormone depletion media supplemented with 1nM Sir-Hoechst ...