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Citations for Cytoskeleton :
101 - 143 of 143 citations for Monobenzyl Phthalate Unlabeled 100 Ug Ml In Methyl Tert Butyl Ether since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Pharmacological treatments included 1 unit/ml Rho Activator I (CN01; Cytoskeleton, Denver, CO), 1 mM TAK165 (Mubritinib ...
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bioRxiv - Cell Biology 2020Quote: Microtubule polymerization assays were performed using 1.5 mg/mL purified tubulin (Cytoskeleton, Inc) that was taken from a 10 mg/mL flash frozen stock ...
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bioRxiv - Neuroscience 2020Quote: ... Starved flies were then moved to tubes with standard corn meal and sugar cane syrup supplemented with taxol (10 nM, 100 nM, 1 µM or 10 µM, Paclitaxel Cytoskeleton Inc.) or DMSO (0.01% ...
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bioRxiv - Cell Biology 2022Quote: Prophase I-arrested oocytes were cultured in vitro in milrinone-free CZB medium supplemented with 100 nM SiR-tubulin (Cytoskeleton #NC0958386) in a humidified ...
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bioRxiv - Biophysics 2022Quote: ... then coated with 6μg/ml of Rhodamine Fibronectin (Cat. # FNR01, CYTOSKELETON, Denver, CO, USA) by incubating at 37°C for 1hr ...
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bioRxiv - Biochemistry 2023Quote: ... microtubules were polymerized from 5 mg mL-1 porcine tubulin (Cytoskeleton Inc., Denver, CO) at 37 °C for 20 min in BRB80 supplemented with 1 mM GTP and then diluted 1:10 in BRB80 + 20 µM taxol ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then washed three times in PBS and stored in PBS containing SIR-actin (100 nM; Cytoskeleton; catalogue number: CY-SC001) at 4°C until imaging ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then washed three times in PBS and stored in PBS containing SiR-actin (100 nM; Cytoskeleton; catalog number: CY-SC001) at 4°C until imaging ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed three times in PBS and stored in PBS containing SiR-actin (100 nM; Cytoskeleton; catalog number: CY-SC001) at 4°C until imaging ...
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bioRxiv - Cell Biology 2021Quote: ... Porcine brain microtubules were prepared from frozen aliquots of 5mg/ml tubulin (Cytoskeleton, Denver, CO) in polymerization buffer (BRB80 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then permeabilized for 10 minutes with 0.1% Triton X-100 and stained with Rhodamine Phalloidin for 30 minutes (Cytoskeleton Inc., Denver, CO, USA). Cells were then washed in DPBS and mounted with DAPI-containing Vectashield mounting media (Vector Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... tubulin was labeled by adding 100 nM SiR-tubulin and 10 µM verapamil for 30-60 minutes prior to imaging (Cytoskeleton, Inc. CY-SC002). Actin was labeled by diluting SPY555-actin (Cytoskeleton ...
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bioRxiv - Cell Biology 2020Quote: ... and then placed in 1.5 ml imaging media containing 1 μM SiR-DNA (CY-SC007, Cytoskeleton) + 10 μM Verapamil (for nucleus staining ...
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bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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bioRxiv - Cell Biology 2022Quote: ... Microtubules were made by mixing 1 μl of HiLyte Fluor 488 tubulin (2 mg/ml, Cytoskeleton), 2 μl biotinylated tubulin (2 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: Microtubules were made by mixing 1.5 μL of 2 mg/mL HiLyte Fluor 488 tubulin (Cytoskeleton), 2 μL of 2 mg/mL ...
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bioRxiv - Biophysics 2023Quote: ... we performed additional tests: incubation with 1.0 μg/ml C3-transferase (RhoA inhibitor, Cytoskeleton, cat. CT04) and with 1.0 μg/ml of RhoA activator (Cytoskeleton ...
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bioRxiv - Biophysics 2021Quote: ... 0.2 mg ml−1 κ-casein and) lacking taxol but including 1 μM SiR-tubulin (Cytoskeleton inc.) and the mixture was allowed to bind to coverslips for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... 0.2 μl of 1 mg/ml HiLyte Fluor 647 or X-rhodamine-labelled tubulin (#TL670M, #TL6290M, Cytoskeleton) and 0.5 μl of 0.5 mg/ml biotin tubulin (#T333P ...
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bioRxiv - Cell Biology 2021Quote: Fluorescent MTs were polymerized at 2 mg/ml by incubating tubulin and rhodamine labeled tubulin (Cytoskeleton Inc.) at 10:1 ratio in BRB80 with 1 mM DTT and 1mM GTP for 5 min on ice ...
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bioRxiv - Biophysics 2021Quote: ... for 24 hours and treated with 5ng/ml TGFβ1 and 100nM SiR-actin kit (Cytoskeleton.Inc, CY-SC001).
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bioRxiv - Biophysics 2019Quote: ... the primary antibodies used in the study were sheep anti-tubulin (2.5 μg mL−1, ATN02, Cytoskeleton) and mouse anti-TOM20 (2.5 μg mL−1 ...
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bioRxiv - Cell Biology 2020Quote: A solution of 5 μg/mL Fibronectin labeled with HiLyte fluor 488 (FNR02-A, Cytoskeleton, Inc. USA) in PBS was incubated on glass bottom petri dish for 30 min at 4°C and rinsed with cold PBS thrice ...
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bioRxiv - Biophysics 2023Quote: ... They are next incubated with a 10 μg/mL rhodamine-labelled fibronectin solution (Cytoskeleton, cat. FNR01-A) for 1h and then dried at room temperature for about 5min ...
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bioRxiv - Cell Biology 2023Quote: Stock actin (10 mg/ml) was diluted 1:10 into ATP-supplemented general actin buffer from Cytoskeleton Inc ...
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bioRxiv - Bioengineering 2023Quote: ... we incubated the glass coverslip with a 500 µg/mL solution of green fluorescent laminin (Cytoskeleton, Inc., LMN02) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Gels were then incubated with a 10 μg/ml laminin solution (1:1 rhodamine labeled (LMN01-A, Cytoskeleton, Inc.) to non-labeled laminin-1 (L2020 ...
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bioRxiv - Cell Biology 2023Quote: ... a reaction vessel was prepared on ice containing 3 mg/ml of >97% purified porcine tubulin (Cytoskeleton, Inc. (#T240)) and the substances being tested ...
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bioRxiv - Molecular Biology 2021Quote: ... the pathway was first inhibited with fasudil 50µM (Bio-Techne, Minnesota, USA) or C3-exoenzyme 2µg/ml (Cytoskeleton, Denver, USA) for 15 hours and then activated with PDGF-AA at 50ng/ml for 20 minutes and proceed to the G-LISA or IF assay of myosin light chain phosphorylation (p-MLC) ...
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bioRxiv - Molecular Biology 2021Quote: ... the pathway was activated with PDGF-AA at 50 ng/ml for 20 minutes at 37°C and the G-LISA assay (Cytoskeleton) was performed following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... of each was incubated for 15 min at 37°C in General Tubulin Buffer with 2.25 mg/mL of 99% pure porcine tubulin and 1 mM GTP (all from Cytoskeleton Inc.), in a final volume of 50 μL ...
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bioRxiv - Cell Biology 2022Quote: ... the medium was replaced by a standard growth medium supplemented with 2% (vol/vol) GFR Matrigel and 10 µg/ml of a fluorescently labeled ECM molecule (HiLyte Fluor 488-labeled fibronectin, Cytoskeleton, Cat Number FNR02 ...
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bioRxiv - Bioengineering 2022Quote: ... The beads were cleaned 3 times in PBS and resuspended in a solution of PBS with 20 μg/ml biotinylated fibronectin (Cytoskeleton) or biotinylated BSA (Pierce ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were cultured for nine days and fluorescent labeling of matrices for direct visualization during live cell microscopy was achieved by adding 1 µg/ml rhodamine-coupled fibronectin (Cytoskeleton) at day three ...
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bioRxiv - Biochemistry 2023Quote: ... was added to either supplemented actin buffer alone or in the presence of 0.4 mg mL-1 G-actin (cat. AKL99, Cytoskeleton Inc.). The final ligand concentration was 2.5 μM ...
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bioRxiv - Cell Biology 2023Quote: ... The coverslips were then rinsed in water and coated with 20 µg/mL fibronectin (1/10 Rhodamine Fibronectin (Cytoskeleton, FNR01), 9/10 fibronectin (FC010 ...
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bioRxiv - Biophysics 2019Quote: ... This is done by mixing 5 μL sonicated GMPCPP microtubule (1 mg/mL short microtubules prepared from 97% pure tubulin (HTS03-A, Cytoskeleton, Inc), stored at −80 °C and thawed right before use ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... the samples were preincubated for either 3 h with Rho/Rac/Cdc42 Activator I (1 µg/ml, Catalog number CN04, Cytoskeleton Inc), 30 min with MEK inhibitor PD 98059 (50 µM ...
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bioRxiv - Biophysics 2022Quote: ... the microstamps were inked for 1 hour at room temperature with 25 μg/mL rhodamine FN solution (Cytoskeleton Inc., Colorado, United States). Deformable silicone Flexwell membranes (Flexcell inc. ...
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bioRxiv - Cell Biology 2020Quote: ... cells were grown at high density (150,000 cells/cm2) for 48 hours and were then treated with Rho-activator-II (Cytoskeleton: 1 µg/ml) for 3 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... Fixed gelatin-coated coverslips were washed six times with CMF-PBS at room temperature and coated with 50 µg/ml HiLyte488 FITC-conjugated fibronectin (Cytoskeleton, Inc., Denver, CO) for one hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... embryos were washed three times with PBS and stained with 2 μg/mL Hoechst 33342 (Nacalai Tesque, 04929-82) and 20 nM Acti-stain™ 555 phalloidin (Cytoskeleton, PHDH1-A) in PBS containing 0.05% Triton X-100 at 4°C overnight in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended in DMEM-1%BSA and incubated in suspension for 30 min at 37°C in 10% CO2 and 2 μg/ml CT04 (#CT04-A, Cytoskeleton or 10 μM EHT1864 (#3872, Tocris). The vehicle control included the corresponding dilution of DMSO as needed ...