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Citations for Cytoskeleton :
101 - 150 of 208 citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... colorimetric Rac1 and RhoA G-LISA Activation Assay Kits (BK128-S and BK124-S Cytoskeleton Inc., Denver CO, USA) were used according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined using the Precision Red Advanced Protein Assay Reagent (Cytoskeleton ADV02). Extracted proteins (25-35 μg ...
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bioRxiv - Cell Biology 2019Quote: ... and protein concentration determined using the Precision Red™ Advanced Protein Assay Reagent (Cytoskeleton). Respective samples were treated with 300nM Nocodazole for 15 min before lysis ...
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bioRxiv - Cell Biology 2022Quote: ... or along with the human recombinant proteins: WASP-VCA domain protein (Cat# VCG03, Cytoskeleton) or ERK3 protein (M31-34G ...
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bioRxiv - Cell Biology 2020Quote: Purified actin protein (Cytoskeleton, Inc.) was used for the in vitro actin polymerization reaction ...
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bioRxiv - Neuroscience 2020Quote: ... and protein concentration estimated in the supernatant using Precision Red Advanced Protein Assay (Cytoskeleton, CA). Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad) ...
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bioRxiv - Immunology 2023Quote: ... Protein concentrations of crude extracts were determined using the Advanced Protein Assay (Cytoskeleton, Denver, USA).
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bioRxiv - Molecular Biology 2020Quote: ... Intracellular Rac1 activity was determined using a Rac1 G-LISA Activation Assay Kit (Colorimetric format) (Cytoskeleton, catalog BK128, Denver, CO) using 25μg total protein per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Intracellular RhoA activity was determined using a RhoA G-LISA Activation Assay Kit (Colorimetric format) (Cytoskeleton, catalog BK124, Denver, CO) using 20μg total protein per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... The seven protein subunits of the Arp2/3 protein complex were purified from bovine brain (Cytoskeleton). The GST-VCA fusion protein associated with the glutathione-agarose 4B (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... and protein concentrations were measured using the Precision Red Advanced Protein Assay Reagent (# ADV02; Cytoskeleton Inc.) and finally equalized with ice-cold lysis buffer to 1.0 mg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... Equalized protein extractions were transferred to a Rho-GTP-binding protein pre-coated plate (Cytoskeleton, Inc.). The plate was placed on a microplate shaker at 300 rpm for 30 min at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... C3 exoenzyme protein (CT03; Cytoskeleton, Inc.) was resuspended and dialyzed into PBS and injected at 1 mg/mL ...
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bioRxiv - Neuroscience 2019Quote: G/F-actin assay was performed according to the manufacturer’s instructions (G/F-actin In Vivo Assay Kit, Cytoskeleton, Denver, CO). G-and F-actin bands on western blots were scanned by densitometry and the ratios of free G-actin to F-actin were calculated.
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bioRxiv - Cell Biology 2023Quote: ... The tubulin was then mixed with both biotin-conjugated and HiLyte 647 (far-red) labeled porcine tubulin (Cytoskeleton Inc., Denver, CO) and resuspended in PEM buffer (80 mM PIPES pH 6.9 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration of the cleared lysates was determined by Precision Red Advanced Protein Assay Reagent (Cytoskeleton Inc.). Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... labeled tubulin with 9 μL unlabeled MTs and transferred to a 37°C water bath for 20 min and stabilized by adding 10 μM paclitaxel (Cytoskeleton, Denver, CO). MTs were stored at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... The COS-7 cells were labelled with PKmito RED (Nanjing Genvivo Biotech Co., Ltd. PKMR-1, CY-SC052)/PKmito DEEP RED (Cytoskeleton, CY-SC055) 15 minutes in DMEM ...
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bioRxiv - Cell Biology 2020Quote: Purified tubulin proteins were obtained from Cytoskeleton, Inc ...
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bioRxiv - Biophysics 2020Quote: The data for the linear actin filaments was taken from 18 so the preparation procedure is identical [as described therein]: 250 μg of rabbit skeletal muscle G-actin (AKL99-A; Cytoskeleton, Inc., Denver, CO) were resuspended to using 250 μL of General Actin Buffer (BSA01 ...
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bioRxiv - Cancer Biology 2020Quote: ... Fixed gelatin-coated coverslips were washed six times with CMF-PBS at room temperature and coated with 50 µg/ml HiLyte488 FITC-conjugated fibronectin (Cytoskeleton, Inc., Denver, CO) for one hour at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: The G-actin/F-actin assay was performed with a G-actin/F-actin in vivo assay kit (Cytoskeleton, Inc., Denver, CO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then permeabilized for 10 minutes with 0.1% Triton X-100 and stained with Rhodamine Phalloidin for 30 minutes (Cytoskeleton Inc., Denver, CO, USA). Cells were then washed in DPBS and mounted with DAPI-containing Vectashield mounting media (Vector Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: The G-actin/F-actin ratio in 229 R cells was assayed using the G-actin/F-actin In Vivo Assay Kit (Cytoskeleton, Denver, CO, USA). 229 R cells were scraped in LAS2 buffer and lysed ...
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bioRxiv - Cell Biology 2019Quote: Soluble G-actin was separated from filamentous F-actin using the In Vivo G-actin/F-actin Assay Kit (Cat# BK037, Cytoskeleton, Inc., Denver, CO) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... cells were rinsed with warmed PBS and cell lysates prepared in cell lysis buffer as instructed and provided by the RhoA or Rac1 pull-down instruction manual and kit (Cytoskeleton, Inc. Denver, CO). Protein quantitation was performed by BCA method and 600 μg of protein was incubated with 50 μg Rhotekin-RBD beads for RhoA pull-down or with 10 µg of PAK-PBD beads for Rac1 pull-down at 4°C for 1 h ...
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bioRxiv - Cell Biology 2020Quote: Microtubules were polymerized from cycled bovine tubulin (PurSolutions—Nashville, TN) mixed with Cy5-labled bovine tubulin (PurSolutions) and stabilized with 10 μM Taxol (Cytoskeleton Inc.—Denver, CO) as previously described (71) ...
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bioRxiv - Physiology 2020Quote: Recombinant tau from bacteria or heat-stable tau fractions isolated from mouse brains or SH-SY5Y cells stably expressing tau were incubated with 30 μM porcine brain tubulin (Cytoskeleton, Denver, CO, USA) in PEM buffer (80 mM PIPES ...
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bioRxiv - Neuroscience 2023Quote: F-actin and G-actin content was measured using the G-actin/F-actin in vivo assay biochemical kit (Cytoskeleton Inc., Denver, CO) as previously described in (Laguesse et al. ...
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bioRxiv - Plant Biology 2019Quote: The VCA domain of human WASP protein was expressed in a bacterial expression system as a GST-tagged fusion protein (Cytoskeleton). The seven protein subunits of the Arp2/3 protein complex were purified from bovine brain (Cytoskeleton) ...
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bioRxiv - Microbiology 2019Quote: ... Whole colonic tissue was homogenized in cell lysis buffer and protein concentration was determined using the Precision Red Protein Assay (Cytoskeleton). Samples were sent to Eve Technologies for analysis using their 32-multiplex Mouse Cytokine/Chemokine Array.
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bioRxiv - Neuroscience 2023Quote: ... The pull-down protein samples were then subjected to western blotting with the whole cell lysate and His-tagged Rac1 protein (#RC01, Cytoskeleton) serving as the controls.
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bioRxiv - Cell Biology 2019Quote: ... HCLE cells were pre-loaded with either CellMask™ Deep Red Plasma membrane stain as described above or 1 μM of SiR-Actin Spirochrome probe (Cytoskeleton Inc., Denver, CO) for 10 minutes at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: ... Lipids accumulated in HLOs and cytoskeleton were stained with 2 μM BODIPY® 493/503 and 1 μM SiR-Actin (Cytoskeleton Inc., CO, USA), respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... 160 nM WASP VCA domain protein (#VCG03, Cytoskeleton) and 60 nM Arp2/3 protein complex (#RP01P ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined using Precision Red (Cytoskeleton) and 60μg of protein were separated using 4-15% SDS-PAGE gels and transferred onto nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... Precision Red Advanced Protein Assay (ADV02-A; Cytoskeleton), calcein AM (C1430 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μM TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 mM GTP (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: Protein concentrations were determined by Bradford (Cytoskeleton, USA). Twenty μg of protein was resolved on polyacrylamide-SDS gels and electro-transferred to PVDF membranes.
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bioRxiv - Developmental Biology 2022Quote: ... Protein concentration was quantified using Precision Red (Cytoskeleton) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Soluble proteins were collected after a 10 minute centrifugation step at 15000 rpm and protein concentration was measured using PrecisionRed (Cytoskeleton; ADV02). 1.5 mg of protein was mixed with 25 μl of pre-equilibrated GFP-Trap_A beads (ChromoTek ...
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bioRxiv - Molecular Biology 2020Quote: ... and 60 nM Arp2/3 protein complex (#RP01P, Cytoskeleton) were also added to the actin polymerization buffer ...
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bioRxiv - Biochemistry 2020Quote: ... was measured using Advanced Protein Assay Reagent (Cytoskeleton, Inc.) with bovine serum albumin as a standard ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc.) and 50 μg total protein were separated on SDS-PAGE followed by western blot ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was measured with Precision Red Assay (Cytoskeleton). Laemmli sample buffer (Biorad ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein was quantified using the Precision Red (#ADV02; Cytoskeleton) advanced protein assay and 10-20μg was used ...
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bioRxiv - Genetics 2024Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc.) and 50 - 100 μg total protein was separated on SDS-PAGE followed by western blot ...
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bioRxiv - Plant Biology 2019Quote: ... The purified Arp2/3 protein complex from porcine brain (Cytoskeleton) and GST-tagged VCA domain of human WASP protein (Cytoskeleton ...
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bioRxiv - Plant Biology 2019Quote: ... and GST-tagged VCA domain of human WASP protein (Cytoskeleton) were used to initiate actin nucleation ...
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bioRxiv - Cell Biology 2023Quote: ... and total protein concentration was measured using Precision Red (Cytoskeleton). Normalized samples were denatured with SDS loading buffer (250mM Tris [pH 6.8] ...