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Citations for Cytoskeleton :
1 - 50 of 52 citations for IL 5 Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... and 5 μM β-actin (human platelets; Cytoskeleton) whose pH was adjusted to 7.25 with KOH ...
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bioRxiv - Cell Biology 2023Quote: Human platelet actin (Cytoskeleton Inc.) was polymerized in F-actin buffer (5 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2019Quote: Human non muscle actin (Cytoskeleton, Inc.) in general actin buffer (5 mM Tris-HCl [pH 8.0] and 0.2 mM CaCl2 ...
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bioRxiv - Biochemistry 2020Quote: ... human platelet actin (Cytoskeleton Inc., APHL99), were mixed in equimolar ratios at 50 μM and incubated on ice for 30 minutes prior to measurement.
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bioRxiv - Cell Biology 2020Quote: Purified human platelet actin (APHL99, Cytoskeleton) was polymerized following the manufacturer’s protocol and attached to polylysine coated coverslips by incubation with 2 µg/ml actin in actin polymerization solution ...
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bioRxiv - Cancer Biology 2023Quote: Human non-muscle actin (Cytoskeleton, # APHL99) was polymerized or de-polymerized according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: Purified (>99%) human actin monomers (Cytoskeleton, APLC99) were prepared (5 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mL DMSO (Cytoskeleton)] was added to the stock microtubule solution at room temperature.
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bioRxiv - Biophysics 2022Quote: ... Lyophilized human cofilin 1 was purchased from Cytoskeleton (CF01) and reconstituted in MB buffer (20 mM MOPS pH 7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... Human full-length gelsolin was purchased from Cytoskeleton (US).
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bioRxiv - Plant Biology 2019Quote: ... and GST-tagged VCA domain of human WASP protein (Cytoskeleton) were used to initiate actin nucleation ...
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bioRxiv - Biophysics 2022Quote: ... and 5 μM tubulin-AlexaFluor647 (Cytoskeleton) were combined with 1mM DTT and 0.6mM GMP-CPP in M2B buffer and incubated spun at 300,000 g to remove aggregates ...
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bioRxiv - Cell Biology 2023Quote: ... 5% biotin-labeled tubulin (Cytoskeleton, T333P), and 90% unlabeled tubulin (Cytoskeleton ...
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bioRxiv - Cell Biology 2023Quote: ... and with different recombinant Cdc42 (3µg of human Cdc42, #CD01 Cytoskeleton; 3µg of recombinant MBP tagged-C ...
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bioRxiv - Cell Biology 2022Quote: ... taxol-stabilized microtubules and recombinant human kinesin (KIF5A) were purchased from Cytoskeleton™ (Denver ...
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bioRxiv - Microbiology 2023Quote: ... 5 µg of purified porcine tubulin (Cytoskeleton) was present in protein mixtures prior to Flag Ab immunoprecipitation.
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bioRxiv - Microbiology 2023Quote: Microtubules with ∼5% biotin–tubulin (Cytoskeleton inc.) and ∼5% Alexa488-labeled or Alexa647-labeled fluorescent tubulin (Cytoskeleton inc. ...
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bioRxiv - Biophysics 2021Quote: ... the buffer also contained 50 µM non-muscle human actin protein (Cytoskeleton, Inc) and 0.2 mM ATP and 0.2 mM CaCl2.
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bioRxiv - Biochemistry 2024Quote: ... Taxol-stabilized human unmodified microtubules assembled with 1.5% biotinylated tubulin (Cytoskeleton, Inc. #T333P) and 1% HiLyte FluorTM 488-labeled tubulin (Cytoskeleton ...
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bioRxiv - Neuroscience 2020Quote: ... Non-muscle actin (5 µM, APHL99, Cytoskeleton Inc.) was included in the pipette to prevent wash-out of plasticity63 ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 μg/mL RhoA Activator II (Cytoskeleton, Inc.) or 2 μg/mL Rho A Inhibitor I (Cytoskeleton ...
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bioRxiv - Cell Biology 2022Quote: ... or along with the human recombinant proteins: WASP-VCA domain protein (Cat# VCG03, Cytoskeleton) or ERK3 protein (M31-34G ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μl of soluble TRITC-labelled tubulin (Cytoskeleton, TL590M) in phase separation buffer containing 500 ng ml−1 nocodazole was added for a final tubulin concentration of 5 μM ...
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bioRxiv - Biophysics 2023Quote: 5 μM porcine brain tubulin (Cytoskeleton, Cat No. T240) and 5 μM MAP7 MTBD were mixed with 2 mM GTP (final concentration ...
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bioRxiv - Biochemistry 2020Quote: ... For measurements including actin (human platelet actin, 99% pure, from Cytoskeleton, Inc., catalog nr: APHL99), the buffer was 5 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... 5 μM F-actin (mixture 20% Biotinylated actin (Cytoskeleton Inc.)) was added to the mixture and incubated at RT for 10 min ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated with monoclonal mouse anti-human RhoA primary antibody (# GL01A; 1:250; Cytoskeleton Inc.), followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02 ...
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bioRxiv - Cell Biology 2021Quote: ... 22 μl of 5 mg ml−1 tubulin protein (Cytoskeleton, T240) was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton ...
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bioRxiv - Microbiology 2023Quote: ... and ∼5% Alexa488-labeled or Alexa647-labeled fluorescent tubulin (Cytoskeleton inc.) were prepared as described64 and stabilized with final 20µM taxol (Cytoskeleton inc.) ...
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bioRxiv - Biophysics 2024Quote: ... GST-tagged WCA domain of human WASP protein (GST-WCA) was purchased from Cytoskeleton (Cat. #VCG03-A) and used without further purification ...
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bioRxiv - Biophysics 2020Quote: ... 5 μL of 10x polymerization buffer solution (BSA02; Cytoskeleton, Inc., Denver, CO) mixed with 45 μL of the previously produced solution were left for 1h at room temperature to polymerize ...
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bioRxiv - Cell Biology 2022Quote: ... 0.4 µl of 5 mg/ml biotin labeled tubulin (Cytoskeleton, #T333P-A), 20% glycerol ...
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bioRxiv - Biophysics 2019Quote: MTs were prepared by polymerizing 5 mg/ml tubulin (Cytoskeleton, Denver, CO) in BRB80 buffer (80 mM PIPES ...
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bioRxiv - Cell Biology 2022Quote: ... in a ratio of 1:5 and then stabilizing with paclitaxel (Cytoskeleton). MTs and test proteins were incubated in binding buffer A (80 mM Pipes ...
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bioRxiv - Biochemistry 2023Quote: ... cells were pre-extracted for 5 minutes with cold CSK buffer (‘Cytoskeleton buffer’ ...
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bioRxiv - Plant Biology 2019Quote: The VCA domain of human WASP protein was expressed in a bacterial expression system as a GST-tagged fusion protein (Cytoskeleton). The seven protein subunits of the Arp2/3 protein complex were purified from bovine brain (Cytoskeleton) ...
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bioRxiv - Microbiology 2020Quote: ... recombinant human cofilin-1 (CFL1 P23528) and arp2/3 complex (ARPC4 Q148J6) from bovine brain were all purchased from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were then incubated with 30 µg of Ras-GTP Binding Domain (RBD of human c-Raf kinase) fused with GST associated with beads (#RF02, Cytoskeleton) at 4°C for 1h ...
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bioRxiv - Immunology 2021Quote: ... cell lysate (125 μg) was added to 5 μg GGA3-PBD beads (Cytoskeleton, USA) to pull down ARFs-GTP and incubated at 4°C on a rotator for 1h and then the beads were extensively washed to remove unbound protein ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: MTs were polymerized by incubating 5 µM tubulin (4% HyLite 647 tubulin (Cytoskeleton #TL670M), ∼1% biotin tubulin (Cytoskeleton #T333P) ...
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bioRxiv - Biochemistry 2023Quote: ... microtubules were polymerized from 5 mg mL-1 porcine tubulin (Cytoskeleton Inc., Denver, CO) at 37 °C for 20 min in BRB80 supplemented with 1 mM GTP and then diluted 1:10 in BRB80 + 20 µM taxol ...
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bioRxiv - Cell Biology 2023Quote: ... rhodamine-labeled MTs were polymerized using a mixture containing 5% rhodamine-labeled tubulin (Cytoskeleton, TL590M), 5% biotin-labeled tubulin (Cytoskeleton ...
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bioRxiv - Molecular Biology 2022Quote: ... or 60min-old Tau:RNA or Tau:RNA:PEG coacervates were mixed with 5 μM porcine tubulin-Alexa594 (Cytoskeleton), and samples were pipetted onto the glass surface of an imaging dish (MaTek ...
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bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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bioRxiv - Cell Biology 2023Quote: ... which was 5% fluorescently labelled with HiLyte 488 or HiLyte 647 conjugated porcine brain tubulin (Cytoskeleton), was assembled at 40 μM at 1 mM GTP for 30 mins at 37°C ...
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bioRxiv - Cell Biology 2020Quote: A solution of 5 μg/mL Fibronectin labeled with HiLyte fluor 488 (FNR02-A, Cytoskeleton, Inc. USA) in PBS was incubated on glass bottom petri dish for 30 min at 4°C and rinsed with cold PBS thrice ...
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bioRxiv - Cell Biology 2020Quote: ... Imaging solution consisted of indicated concentration of porcine brain tubulin (5% or 15% rhodamine-labeled, Cytoskeleton Inc., Denver CO) and Alexa488-labeled tau in BRB80 supplemented with 1 mM GTP ...
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bioRxiv - Cell Biology 2022Quote: ... before incubating for 5 minutes at room temperature in 40 ng/µl rhodamine-labeled actin (Cat# AR05, Cytoskeleton, Inc.) freshly prepared in cytoskeletal buffer ...
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bioRxiv - Biochemistry 2021Quote: Porcine Tubulin at 1:5 ratio (rhodamine-labeled: unlabeled) (cat. no. T240 and TL590M-A respectively, Cytoskeleton, Inc., Denver, USA) was reconstituted to 4.0 mg/mL in cold polymerization buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM guanidine carbonate and 1.2 mM ATP (from Actin Polymerization Buffer, cat. BSA02, and ATP, cat. BSA04, Cytoskeleton Inc.). Samples were incubated at 37 °C for 2 h.