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Citations for Cytoskeleton :
201 - 250 of 315 citations for Human Uromodulin Like 1 UMODL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The cells were labeled with the dyes SPY555-Actin (Cytoskeleton; 1:1,000) and NucSpot Live 650 (Biotium ...
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bioRxiv - Cancer Biology 2021Quote: ... the Rac1- and cdc42 Small GTPase Activation Assay kits (G-LISA) were used (027BK127-S, 027BK128-S, Cytoskeleton, Inc) following the company’s guidelines.
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bioRxiv - Cell Biology 2020Quote: ... and G-LISA assay was performed as per the manufacturer’s instructions (Rac1 and Cdc42 G-LISA activation assay kits, Cytoskeleton).
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bioRxiv - Cell Biology 2021Quote: The pyrene-actin polymerization and depolymerization assays were performed using the Actin Polymerization Biochem Kit (Cytoskeleton, Inc., Denver, CO) following the manufacturer protocol ...
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bioRxiv - Systems Biology 2022Quote: The Rho activity was measured and quantified by using the RhoA Activation Assay Biochem Kit (cat. no. BK036; Cytoskeleton) based on the Rhotekin pull-down assay as per the manufacturer’s instructions as described previously(39) ...
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bioRxiv - Biochemistry 2022Quote: ... colorimetric Rac1 and RhoA G-LISA Activation Assay Kits (BK128-S and BK124-S Cytoskeleton Inc., Denver CO, USA) were used according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2021Quote: F-actin disruption assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem kit (Cytoskeleton, USA) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Actin protein levels were calculated using a G-actin/F-actin in vivo Assay Biochem Kit as per manufacturer’s instructions (Cytoskeleton). Briefly ...
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bioRxiv - Immunology 2021Quote: ... GTP-bound form of CDC42 was pulled down for analysis using the Cdc42 Pull-Down Activation Assay Kit (Cytoskeleton). FLAG-tagged protein pull-down assays were performed using DDDDK tagged protein purification kit (MBL) ...
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bioRxiv - Cancer Biology 2023Quote: GTP-bound RAS levels were assessed with a RAS G-LISA Assay Kit per manufacturer’s instructions (Cytoskeleton INC., #BK131). 9 ξ 105 cells were plated in a 3D 24-well plate ...
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bioRxiv - Cell Biology 2023Quote: ... GTP-bound ARF6 was detected in cell lysates using the ARF6 Activation Assay Biochem Kit™ (Cytoskeleton, BK-033) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies used were as follows: mouse anti-RAC1 (Cytoskeleton #ARC03, 1:250); mouse anti-RHOA (Cytoskeleton #ARH05 ...
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bioRxiv - Cell Biology 2021Quote: ... Pharmacological treatments included 1 unit/ml Rho Activator I (CN01; Cytoskeleton, Denver, CO), 1 mM TAK165 (Mubritinib ...
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bioRxiv - Cell Biology 2021Quote: ... pH adjusted to 6.9 with KOH] supplemented with 1 mM GTP (Cytoskeleton BST06) and kept on ice ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used: rabbit anti-cofilin (Cytoskeleton ACFL02; 1:500), rabbit anti-Ser3-phosphorylated cofilin (Cell Signaling Technology #3311 ...
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bioRxiv - Biophysics 2021Quote: Cells were incubated with 1-2 μM of SiR-Actin (Cytoskeleton, Inc. Spirochrome) for 15-30 min in calcium-free buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Solutions of 434 nM His-CLASP2α or 1 μM α-actinin (Cytoskeleton, Inc.) were incubated with and without 15 μM phalloidin-stabilized F-actin for 20 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... The following stains were also used: ActiStain Phalloidin (Phdh1, Cytoskeleton Inc, 1:500), DAPI (Thermoscientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies used were mouse anti-Rac1 (1:500, Cytoskeleton Inc, #ARC03) and rabbit anti-GAPDH (1:3000 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in MEDIA supplemented with 1 μM SiR-Tubulin (Cytoskeleton, Inc) for 30-60 minutes prior to washing out the SiR-Tubulin with fresh DMEM before imaging ...
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bioRxiv - Neuroscience 2023Quote: Cultured ependymocytes were treated with 1 μM Sir-Actin (Cytoskeleton, Denver, CO, USA) for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... The media was replaced with fresh media containing MemGlow 488 (Cytoskeleton; 1:200) and cells were incubated for 20 min at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: For the determination of active GTP bound form of Cdc42 we used the G-LISA kit for Cdc42 from Cytoskeleton according to the protocol of the manufacturer.
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bioRxiv - Biophysics 2020Quote: ... Rac1-GTP and Cdc42-GTP using the RhoA and Cdc42 pull-down activation assay biochem kit (PN:BK036-S and BK034-S, Cytoskeleton, Inc.) following the protocol provided by the manufacturer ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: Globular and filamentous actin ratios were determined by western blot as described by commercially available G-actin/ F-actin In Vivo Assay Kit (Cytoskeleton). Globular and filamentous immunocytochemistry was performed as previously described [53] ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysate preparation and measurement of RHOA-GTP levels were carried out with the RHOA G-LISA activation assay kit (#BK124, Cytoskeleton) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: G- and F-actin were fractionated and enriched using a commercial in vivo assay biochem kit (Cytoskeleton Inc, Colorado, USA), according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Molecular Biology 2020Quote: ... Intracellular Rac1 activity was determined using a Rac1 G-LISA Activation Assay Kit (Colorimetric format) (Cytoskeleton, catalog BK128, Denver, CO) using 25μg total protein per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Intracellular RhoA activity was determined using a RhoA G-LISA Activation Assay Kit (Colorimetric format) (Cytoskeleton, catalog BK124, Denver, CO) using 20μg total protein per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... In vitro microtubule-binding assay was performed using the microtubule-binding protein spin down assay kit (Cytoskeleton, Denver, CO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: The protocol of G and F actin measurement mostly followed the instructions of the “G-actin /F-actin In Vivo Assay Kit” from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2022Quote: ... and Rac or Rho activity was assessed in duplicate using the G-LISA small G-protein activation assay kit (BK128, BK124, Cytoskeleton) according to the manufacturer’s directions ...
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bioRxiv - Cell Biology 2023Quote: RhoAGTP and Rac1GTP pull down as-says were performed using the RhoA and Rac1 Activation Assay Biochem Kit (Cytoskeleton BK030) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... a highly specific fluorescent dye for F-actin (1:250, Cytoskeleton, Inc., CY-SC001), for 3 hours at room temperature ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... labeled at a 1:40 ratio with HiLyte Fluor 647 tubulin (Cytoskeleton, Denver, CO), were flowed into the chamber and immobilized by interaction with rigor kinesin-1E236A ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... Actin-F was labelled with Sir-Actin dye (1:5000, Cytoskeleton, Denver, CO, USA) for 4 h prior to infection.
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bioRxiv - Cell Biology 2023Quote: ... labeled at a 1:40 ratio with HiLyte Fluor 488 tubulin (Cytoskeleton, Denver, CO), were flowed into the chamber and immobilized by interaction with rigor kinesin-1E236A.
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bioRxiv - Biochemistry 2023Quote: ... microtubules were polymerized from 5 mg mL-1 porcine tubulin (Cytoskeleton Inc., Denver, CO) at 37 °C for 20 min in BRB80 supplemented with 1 mM GTP and then diluted 1:10 in BRB80 + 20 µM taxol ...
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bioRxiv - Neuroscience 2023Quote: ... F-actin staining was done using Acti-stain 555 phalloidin (1:2000, Cytoskeleton, PHDH1A) and counter stained with DAPI.
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bioRxiv - Immunology 2021Quote: Activation of ARF6 (ARF6-GTP) was quantitated using the Arf6 Pull-Down Activation Assay Biochem Kit from Cytoskeleton (BK033-S, USA) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: G/F-actin assay was performed according to the manufacturer’s instructions (G/F-actin In Vivo Assay Kit, Cytoskeleton, Denver, CO). G-and F-actin bands on western blots were scanned by densitometry and the ratios of free G-actin to F-actin were calculated.
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bioRxiv - Microbiology 2022Quote: ... 110 μl of LAS2 buffer was prepared according to the protocol of a G-actin/F-actin In Vivo Assay Biochem Kit (Cytoskeleton, Inc.) and mixed with the pellet ...
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bioRxiv - Cell Biology 2023Quote: The F-actin/G-actin ratio was measured using G-Actin/F-Actin In Vivo Assay Biochem Kit (#BK037, Cytoskeleton, Inc.), following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: F- and G-actin fractions from lysates were prepared using a G-Actin/F-Actin in Vivo Assay Biochem kit (Cytoskeleton, #BK037) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Western blots to determine the ratio of G-actin to F-actin were performed on retinal lysates that were processed using a G-actin/F-actin In Vivo Assay Kit (Cytoskeleton, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... NIH3T3 cells were imaged using four wavelengths (405 nm for tagBFP; 488 nm for Citrine; 561 nm for mCherry or FusionRed; 674 nm for SiR700-Tubulin Kit, #CY-SC014, Cytoskeleton Inc.).
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bioRxiv - Neuroscience 2024Quote: Active GTP-bound GTPase levels in brain lysates were measured using G-LISA activation assay kits for Rac1 (Cytoskeleton, Inc., BK128) and RhoA (Cytoskeleton ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated for 30 min with 0.5 μM of SiR-actin or 60 min with 0.5 μM of SiR-tubulin and 10 μM of verapamil in culture medium (SiR Cytoskeleton Kit, Spirochrome SC006).
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bioRxiv - Neuroscience 2020Quote: RhoA activation was assessed with the G-LISA activation kit in accordance with the manufacturer’s instructions (Cytoskeleton Inc., catalog no. BK124-S). Active GTP-bound RhoA was detected with HRP-conjugated anti-RhoA antibodies ...
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bioRxiv - Cell Biology 2023Quote: Measurements of the microtubule-stimulated ATPase activities of KIF1A MD-NC-CC1-FHA and various mutants were performed by using the HTS Kinesin ATPase Endpoint Assay Biochem Kit (Cytoskeleton, Inc., BK053). Briefly ...