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Citations for Cytoskeleton :
51 - 100 of 260 citations for Human Putative Alpha 1 Antitrypsin Related Protein SERPINA2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: 0.4 µM Myosin Motor protein S1 Fragment (Rabbit Skeletal Muscle, Cytoskeleton #CS-MYS04) was incubated either alone or with 2 µM UCS proteins for 10 minutes either at 42°C or on ice in Mg-buffer (10mM Hepes-KOH ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... and Tyr-phosphorylated proteins were collected using Anti-phosphotyrosine Affinity Beads (Cytoskeleton, USA). Proteins were concentrated using the TCA/acetone precipitation method (Fic et al. ...
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bioRxiv - Biochemistry 2024Quote: ... we used a MT binding protein spin-down assay kit (# BK029, Cytoskeleton, Inc.) according to the manufacturer’s instructions ...
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Gα13 loss promotes tumor progression in the KPC transgenic mouse model of advanced pancreatic cancerbioRxiv - Cancer Biology 2021Quote: ... and the protein concentration was determined using Precision Red solution (Cytoskeleton, Inc., Denver, CO) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... MT assays were performed using the MT Binding Protein Spin-down kit from Cytoskeleton, Inc ...
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bioRxiv - Cell Biology 2023Quote: Actin binding and polymerization were assessed using the actin binding protein kit (Cytoskeleton, BK013). Actin pellet gel bands were quantified by densitometry using FIJI.
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bioRxiv - Pathology 2023Quote: ... and the protein concentration was determined using Precision Red solution (Cytoskeleton, Inc., Denver, CO). Equal amounts of protein were separated with a 10% SDS-PAGE electrophoresis gel ...
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bioRxiv - Plant Biology 2020Quote: ... 13 nM Arp2/3 protein complex and 15 nMWASp-VCA domain-GST purified (Cytoskeleton, USA) along with freshly purified ArF-BAR protein in concentrations of 200 ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal protein levels were confirmed by a control western blot of rabbit anti-Kinesin (Cytoskeleton) from the cleared lysates ...
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bioRxiv - Cell Biology 2022Quote: The Microtubule Binding Protein Spin-down assay was performed according to the manufacturers’ protocol (Cytoskeleton). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... Pyrene-labeled actin was incubated with recombinant ARP2/3 protein complex alone (Cat# RP01P, Cytoskeleton) or along with the human recombinant proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolation of ubiquitinated proteins was performed with the Signal-Seeker Ubiquitin Enrichment kit (Cytoskeleton). Cell fractionation was performed with the Qproteome Cell Compartment kit (Qiagen).
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bioRxiv - Cancer Biology 2023Quote: ... Rabbit polyclonal antibody recognizing the Kif11/Eg5 protein (Cat. #AKIN03, discontinued) was purchased from Cytoskeleton, Inc ...
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bioRxiv - Neuroscience 2023Quote: ... or Cdc42 activation in vivo assay biochemical kits for the respective protein (Cytoskeleton Inc., Denver, CO). The tissues were lysed in lysis buffer (50mM Tris-HCl pH7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred to a clean tube and quantified using PrecisionRed (Cytoskeleton; ADV02-A) at OD600.
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bioRxiv - Biochemistry 2024Quote: ... The cells were lysed and protein lysates were used for G-LISA analysis per manufacturer’s (Cytoskeleton) instructions ...
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bioRxiv - Plant Biology 2020Quote: The actin polymerization modulation activity of the proteins was checked using Actin Polymerization Biochem Kit (Cytoskeleton, USA), using the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: The microtubules binding experiments were performed with the Microtubule Binding Protein Spin-Down Assay Biochem Kit (Cytoskeleton, Inc.) following manufacturer protocol.
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bioRxiv - Neuroscience 2023Quote: Actin bundling assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem Kit (Cytoskeleton Inc.) according to protocol of the manufacturer.
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bioRxiv - Systems Biology 2020Quote: ... 300µg of total cell protein was used to pull-down GTP-bound RAS/RAF-RBD complexes according to the manufacturer’s instructions (Cytoskeleton). Activated RAS or 20µg of total cell protein were separated using NuPAGE Novex Bis-Tris gels (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: F-actin disruption assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem kit (Cytoskeleton, USA) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were spun at maximum speed for 10mins before protein level was measured using Precision Red (Cytoskeleton, Inc., #ADV02). Samples were run using precast 4-12% NuPAGE Bis-Tris Acrylamide Gels (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated with monoclonal mouse anti-human RhoA primary antibody (# GL01A; 1:250; Cytoskeleton Inc.), followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02 ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... In vitro microtubule-binding assay was performed using the microtubule-binding protein spin down assay kit (Cytoskeleton, Denver, CO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... F-actin and G-actin protein samples were collected according to the manufacturer’s instructions (#BK037, Cytoskeleton Inc., Denver, CO, USA). F-actin and G-actin were quantified by SDS-PAGE and western blot using actin antibody (#BK037 ...
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bioRxiv - Cell Biology 2020Quote: Muscle homogenates were prepared in buffer A as described above and an equal amount of protein from each sample was incubated with 20 µL of Rhotekin-RBD beads (Cytoskeleton) for 2 hours at 4°C and washed 4 times in ice-cold buffer containing 50 mM Tris (pH 7.4) ...
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bioRxiv - Cell Biology 2022Quote: ... and Rac or Rho activity was assessed in duplicate using the G-LISA small G-protein activation assay kit (BK128, BK124, Cytoskeleton) according to the manufacturer’s directions ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected upon 15min of centrifugation at >15000 rpm at 4°C and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). Equal amounts of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: Human platelet actin (Cytoskeleton Inc.) was polymerized in F-actin buffer (5 mM Tris-HCl ...
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bioRxiv - Microbiology 2019Quote: ... 5 μL of the filtered supernatant was pipetted into a clear flat-bottom UV plate and 250 μL of 1x Advanced Protein Assay Reagent (Cytoskeleton Inc., CO) was added and mixed ...
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bioRxiv - Cancer Biology 2019Quote: Human non muscle actin (Cytoskeleton, Inc.) in general actin buffer (5 mM Tris-HCl [pH 8.0] and 0.2 mM CaCl2 ...
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bioRxiv - Biochemistry 2020Quote: ... human platelet actin (Cytoskeleton Inc., APHL99), were mixed in equimolar ratios at 50 μM and incubated on ice for 30 minutes prior to measurement.
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bioRxiv - Cell Biology 2020Quote: Purified human platelet actin (APHL99, Cytoskeleton) was polymerized following the manufacturer’s protocol and attached to polylysine coated coverslips by incubation with 2 µg/ml actin in actin polymerization solution ...
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bioRxiv - Cancer Biology 2023Quote: Human non-muscle actin (Cytoskeleton, # APHL99) was polymerized or de-polymerized according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The integrated signal measured from the area of the dynein bands is extracted using ImageJ and calibrated using known masses of purified dynein protein (CS-DN01, Cytoskeleton, Denver, CO, USA) that serve as standards ...
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bioRxiv - Genetics 2022Quote: Purified (>99%) human actin monomers (Cytoskeleton, APLC99) were prepared (5 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... MT affinity/dead-head spin was performed by binding motor protein to newly polymerized MTs (unlabeled, 2uM Paclitaxel (Taxol (Cytoskeleton, Denver, CO, US, TXD01) -stabilized ...
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bioRxiv - Microbiology 2020Quote: ... recombinant human cofilin-1 (CFL1 P23528) and arp2/3 complex (ARPC4 Q148J6) from bovine brain were all purchased from Cytoskeleton Inc ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μM β-actin (human platelets; Cytoskeleton) whose pH was adjusted to 7.25 with KOH ...
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bioRxiv - Molecular Biology 2020Quote: ... Human full-length gelsolin was purchased from Cytoskeleton (US).
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bioRxiv - Cell Biology 2023Quote: ... and with different recombinant Cdc42 (3µg of human Cdc42, #CD01 Cytoskeleton; 3µg of recombinant MBP tagged-C ...
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bioRxiv - Cell Biology 2022Quote: ... taxol-stabilized microtubules and recombinant human kinesin (KIF5A) were purchased from Cytoskeleton™ (Denver ...
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bioRxiv - Biochemistry 2024Quote: ... Taxol-stabilized human unmodified microtubules assembled with 1.5% biotinylated tubulin (Cytoskeleton, Inc. #T333P) and 1% HiLyte FluorTM 488-labeled tubulin (Cytoskeleton ...
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bioRxiv - Biochemistry 2020Quote: ... For measurements including actin (human platelet actin, 99% pure, from Cytoskeleton, Inc., catalog nr: APHL99), the buffer was 5 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were then incubated with 30 µg of Ras-GTP Binding Domain (RBD of human c-Raf kinase) fused with GST associated with beads (#RF02, Cytoskeleton) at 4°C for 1h ...
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bioRxiv - Neuroscience 2021Quote: ... p-ERK1/2 (CST, 9101, 1:1000), AKT (CST, 9272, 1:1000), p-AKT (CST, 9275, 1:1000) and Cofilin (Cytoskeleton, ACFL02, 1:500) were used to quantify protein levels ...
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bioRxiv - Cell Biology 2023Quote: ... and 1 μg ml-1 for Rho activator II (CNO3, Cytoskeleton). Cells were treated at least 2h at 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... MCAK (1:1000, Cytoskeleton), EB1 (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μM VCA (Cytoskeleton Inc.) and 250 nM rhodamine-actin were loaded onto flow chambers with or without 1mM GTP and subjected to TIRF imaging immediately ...