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Citations for Cytoskeleton :
1 - 50 of 346 citations for F actin uncapping protein LRRC16A CARMIL1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... F-actin (Cytoskeleton, Inc ...
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bioRxiv - Neuroscience 2021Quote: ... hippocampal slices previously stabilized for 1 h with oxygenated ACSF were lysed and homogenized in the presence of conditions that stabilize F-actin and G-actin using an F/G actin commercial assay (F/G actin in vivo assay BK037, Cytoskeleton Inc., USA). Then ...
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bioRxiv - Cell Biology 2024Quote: ... Alpha-SMA protein was polymerized into filaments (F-actin) together with Rhodamine muscle actin (Cytoskeleton) by adding 10x polymerization buffer (250 mM Tris-HCl ...
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bioRxiv - Neuroscience 2024Quote: ... G- and F-actin was detected by G-Actin/F-Actin In Vivo Assay Biochem Kit (Cytoskeleton) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: The F-actin/G-actin ratio was measured using G-Actin/F-Actin In Vivo Assay Biochem Kit (#BK037, Cytoskeleton, Inc.), following the manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: Mouse podocytes were lysed and examined for their total G-actin and F-actin levels using a G-actin/F-actin In vivo Biochemical Assay Kit (Cytoskeleton, CO). Briefly ...
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bioRxiv - Cell Biology 2024Quote: Intracellular G-actin and F-actin was separated using the G-Actin/F-Actin In Vivo Assay Biochem Kit (Cytoskeleton, BK037) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... F-actin and G-actin were quantified by SDS-PAGE and western blot using actin antibody (#BK037, Cytoskeleton Inc.).
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bioRxiv - Neuroscience 2024Quote: F-actin and G-actin content was measured using the G-actin/F-actin in vivo assay biochemical kit (Cytoskeleton Inc., Denver, CO) as previously described in (Laguesse et al. ...
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bioRxiv - Immunology 2020Quote: The protocol of G and F actin measurement mostly followed the instructions of the “G-actin /F-actin In Vivo Assay Kit” from Cytoskeleton Inc ...
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bioRxiv - Biophysics 2021Quote: ... 5 μM F-actin (mixture 20% Biotinylated actin (Cytoskeleton Inc.)) was added to the mixture and incubated at RT for 10 min ...
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bioRxiv - Plant Biology 2021Quote: F-actin disruption assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem kit (Cytoskeleton, USA) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... F-actin and G-actin protein samples were collected according to the manufacturer’s instructions (#BK037, Cytoskeleton Inc., Denver, CO, USA). F-actin and G-actin were quantified by SDS-PAGE and western blot using actin antibody (#BK037 ...
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bioRxiv - Immunology 2024Quote: ... The G-actin/F-actin assay biochem kit (#BK037; Cytoskeleton, Inc.) was used for F/G actin fractionation experiments ...
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bioRxiv - Cancer Biology 2021Quote: The G-actin/F-actin ratio in 229 R cells was assayed using the G-actin/F-actin In Vivo Assay Kit (Cytoskeleton, Denver, CO, USA). 229 R cells were scraped in LAS2 buffer and lysed ...
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bioRxiv - Biophysics 2022Quote: The G-actin/F-actin In Vivo Assay Kit (cat. #BK037, Cytoskeleton) was used to determine the F/G-actin ratio ...
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bioRxiv - Bioengineering 2022Quote: ... For F-actin staining by SiR-Actin Kit (#CY-SC001, Cytoskeleton, Inc.), the staining solution was prepared by diluting SiR-actin and verapamil (a broad-spectrum efflux pump inhibitor ...
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bioRxiv - Cell Biology 2022Quote: ... F-actin was prepared by first pre-clarifying G-actin (Cytoskeleton, Inc.) by spinning the sample at 13,300 rpm at 4C for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volumes of G-actin and F-actin lysates were analysed by SDS-PAGE and immunoblotting with anti-actin rabbit polyclonal antibody (Cat# AANO1, Cytoskeleton). Densitometric analyses of the G-actin and F-actin levels were performed using ImageJ software ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 1xPBS and lysed in appropriate volume of lysis and F-actin stabilization buffer supplemented with 1mM ATP and protease inhibitor mixture for F-actin stabilization (Cytoskeleton). Lysates were centrifuged at 100 000 x g at 37°C for 1 h to separate the G-actin fraction in the supernatants and pelleted F- actin ...
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bioRxiv - Cell Biology 2022Quote: ... F-actin from bovine cardiac muscle (AD99, Cytoskeleton) and purified His-MBP-NRIP mixed in polymerization buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... All iPSC-CMs were labeled for F-actin with SiR-Actin (Cytoskeleton, Inc.), prior to imaging (0.5 μM ...
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bioRxiv - Cell Biology 2020Quote: G- and F-actin were prepared from rabbit skeletal muscle actin (AKL99, Cytoskeleton) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... F-actin was polymerized from chicken gizzard (smooth muscle) muscle actin (Cytoskeleton, APHL99).
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bioRxiv - Biophysics 2024Quote: ... To prepare 25% biotin-F-actin seeds biotinylated actin monomers (Cytoskeleton, AB07-C) were co-polymerized at a 1:4 molar ration with unlabeled monomers with a total concentration of 10 μM for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: Hippocampal and cortical tissue from WT and HTZ mice were lysed and homogenized in conditions that stabilize filamentous (F-) and monomeric (G-) actin using an F/G actin commercial assay (Cytoskeleton Inc.). Lysed extracts were ultracentrifuged at 100,000 g for 1 h at 37 °C in order to separate pellets (F-actin ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... Actin-F was labelled with Sir-Actin dye (1:5000, Cytoskeleton, Denver, CO, USA) for 4 h prior to infection.
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bioRxiv - Cell Biology 2022Quote: ... phalloidin-stabilized F-actin was prepared by incubating 20 μM G-actin (Cytoskeleton, Inc.) with equimolar phalloidin ...
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bioRxiv - Cell Biology 2023Quote: ... F-Actin was stained using Actistain (1:150, Cytoskeleton) for 1h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... stained for F-actin (100 nM, phalloidin, PHDH1, Cytoskeleton), E-cadherin (5 µg/ml ...
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bioRxiv - Bioengineering 2022Quote: ... Alexa Fluor 568 phalloidin was used to stain filamentous actin (F-actin) (3.5:500, Cytoskeleton). For detection of mucus secreting goblet cells ...
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bioRxiv - Neuroscience 2023Quote: ... we used the G-actin/F-actin in vivo assay kit (Cytoskeleton Inc., Denver, USA) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... F-actin was labeled with rhodamine-phalloidin (Cytoskeleton, Inc. PHDR1) for 20 min and followed by 3x PBS wash ...
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bioRxiv - Cell Biology 2021Quote: ... Tissue sections were stained for F-actin by phalloidin (Cytoskeleton), ezrin (Santa Cruz Biotechnology) ...
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bioRxiv - Neuroscience 2023Quote: F- and G-actin fractions from lysates were prepared using a G-Actin/F-Actin in Vivo Assay Biochem kit (Cytoskeleton, #BK037) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Western blots to determine the ratio of G-actin to F-actin were performed on retinal lysates that were processed using a G-actin/F-actin In Vivo Assay Kit (Cytoskeleton, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... F-actin filaments and the nuclei were labeled with 1 µM of SiR-actin (Cytoskeleton, Denver, USA) and 1 µg/ml of Hoechst 33342 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... All samples were processed using the “G-actin / F-actin In Vivo Assay Kit” (Cytoskeleton Inc., #BK037) which involves lysing cells in a filament-supporting buffer ...
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bioRxiv - Cell Biology 2024Quote: In vitro binding of Maspin to F-actin was performed using rabbit skeletal muscle actin (Cytoskeleton, #AKL99). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... and F-actin with Acti-stain 670 phalloidin (#PHDN1-A, Cytoskeleton).
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bioRxiv - Cell Biology 2020Quote: Purified actin protein (Cytoskeleton, Inc.) was used for the in vitro actin polymerization reaction ...
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bioRxiv - Neuroscience 2021Quote: ... The F-actin pellet was resuspended in a depolymerizing buffer (Cytoskeleton Inc) and then F-and G-actin fractions were diluted in loading buffer (50 mM Tris–HCl ...
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bioRxiv - Cell Biology 2022Quote: ... the purified His-MBP-NRIPs were incubated with F-actin (AD99, Cytoskeleton) in 1 ml of IP buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: Actin protein levels were calculated using a G-actin/F-actin in vivo Assay Biochem Kit as per manufacturer’s instructions (Cytoskeleton). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... Filamentous actin (F-actin) was labeled using Acti-stain 488 phalloidin or Acti-stain 555 phalloidin (1:200; Cytoskeleton). This thorough immunofluorescence staining protocol ensures accuracy and reliability ...
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bioRxiv - Neuroscience 2022Quote: ... Day 8 iTF-Microglia were stained for F-actin using 25 nM SiR-actin (Cytoskeleton, Inc; Cat. No. CY-SC001) probe ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: Globular and filamentous actin ratios were determined by western blot as described by commercially available G-actin/ F-actin In Vivo Assay Kit (Cytoskeleton). Globular and filamentous immunocytochemistry was performed as previously described [53] ...
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bioRxiv - Neuroscience 2021Quote: ... F-actin pellet was resuspended in a depolymerizing buffer (BK037, Cytoskeleton Inc., USA) and then F- and G-actin fractions were diluted in loading buffer (50 mM Tris–HCl ...
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bioRxiv - Microbiology 2022Quote: ... 110 μl of LAS2 buffer was prepared according to the protocol of a G-actin/F-actin In Vivo Assay Biochem Kit (Cytoskeleton, Inc.) and mixed with the pellet ...
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bioRxiv - Developmental Biology 2021Quote: ... a highly specific fluorescent dye for F-actin (1:250, Cytoskeleton, Inc., CY-SC001), for 3 hours at room temperature ...