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Citations for Cytoskeleton :
1 - 50 of 162 citations for Cyclic AMP Direct Chemiluminescent ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Commercial ELISA kits were purchased from Cytoskeleton (G-LISA) to quantify RhoA and Rac1 activation (Cytoskeleton) ...
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bioRxiv - Cancer Biology 2022Quote: RAS activation was assessed by ELISA using a commercially available kit (Cytoskeleton: BK131) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and RAC1 were performed through the use of an ELISA-based Rho-GTPase activation assay kit (Cytoskeleton, Inc, catalog# BK124, BK127, BK128). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were cultured for nine days and fluorescent labeling of matrices for direct visualization during live cell microscopy was achieved by adding 1 µg/ml rhodamine-coupled fibronectin (Cytoskeleton) at day three ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mL DMSO (Cytoskeleton)] was added to the stock microtubule solution at room temperature.
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bioRxiv - Biophysics 2022Quote: ... and 5 μM tubulin-AlexaFluor647 (Cytoskeleton) were combined with 1mM DTT and 0.6mM GMP-CPP in M2B buffer and incubated spun at 300,000 g to remove aggregates ...
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bioRxiv - Cell Biology 2023Quote: ... 5% biotin-labeled tubulin (Cytoskeleton, T333P), and 90% unlabeled tubulin (Cytoskeleton ...
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bioRxiv - Microbiology 2023Quote: ... 5 µg of purified porcine tubulin (Cytoskeleton) was present in protein mixtures prior to Flag Ab immunoprecipitation.
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bioRxiv - Microbiology 2023Quote: Microtubules with ∼5% biotin–tubulin (Cytoskeleton inc.) and ∼5% Alexa488-labeled or Alexa647-labeled fluorescent tubulin (Cytoskeleton inc. ...
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bioRxiv - Neuroscience 2020Quote: ... Non-muscle actin (5 µM, APHL99, Cytoskeleton Inc.) was included in the pipette to prevent wash-out of plasticity63 ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μM β-actin (human platelets; Cytoskeleton) whose pH was adjusted to 7.25 with KOH ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 μg/mL RhoA Activator II (Cytoskeleton, Inc.) or 2 μg/mL Rho A Inhibitor I (Cytoskeleton ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μl of soluble TRITC-labelled tubulin (Cytoskeleton, TL590M) in phase separation buffer containing 500 ng ml−1 nocodazole was added for a final tubulin concentration of 5 μM ...
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bioRxiv - Biophysics 2023Quote: 5 μM porcine brain tubulin (Cytoskeleton, Cat No. T240) and 5 μM MAP7 MTBD were mixed with 2 mM GTP (final concentration ...
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bioRxiv - Biophysics 2021Quote: ... 5 μM F-actin (mixture 20% Biotinylated actin (Cytoskeleton Inc.)) was added to the mixture and incubated at RT for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... 22 μl of 5 mg ml−1 tubulin protein (Cytoskeleton, T240) was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton ...
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bioRxiv - Microbiology 2023Quote: ... and ∼5% Alexa488-labeled or Alexa647-labeled fluorescent tubulin (Cytoskeleton inc.) were prepared as described64 and stabilized with final 20µM taxol (Cytoskeleton inc.) ...
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bioRxiv - Biophysics 2020Quote: ... 5 μL of 10x polymerization buffer solution (BSA02; Cytoskeleton, Inc., Denver, CO) mixed with 45 μL of the previously produced solution were left for 1h at room temperature to polymerize ...
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bioRxiv - Cell Biology 2022Quote: ... 0.4 µl of 5 mg/ml biotin labeled tubulin (Cytoskeleton, #T333P-A), 20% glycerol ...
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bioRxiv - Biophysics 2019Quote: MTs were prepared by polymerizing 5 mg/ml tubulin (Cytoskeleton, Denver, CO) in BRB80 buffer (80 mM PIPES ...
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bioRxiv - Cell Biology 2022Quote: ... in a ratio of 1:5 and then stabilizing with paclitaxel (Cytoskeleton). MTs and test proteins were incubated in binding buffer A (80 mM Pipes ...
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bioRxiv - Biochemistry 2023Quote: ... cells were pre-extracted for 5 minutes with cold CSK buffer (‘Cytoskeleton buffer’ ...
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bioRxiv - Cell Biology 2019Quote: ... Equalized protein extractions were transferred to a Rho-GTP-binding protein pre-coated plate (Cytoskeleton, Inc.). The plate was placed on a microplate shaker at 300 rpm for 30 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... cell lysate (125 μg) was added to 5 μg GGA3-PBD beads (Cytoskeleton, USA) to pull down ARFs-GTP and incubated at 4°C on a rotator for 1h and then the beads were extensively washed to remove unbound protein ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: MTs were polymerized by incubating 5 µM tubulin (4% HyLite 647 tubulin (Cytoskeleton #TL670M), ∼1% biotin tubulin (Cytoskeleton #T333P) ...
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bioRxiv - Biochemistry 2023Quote: ... microtubules were polymerized from 5 mg mL-1 porcine tubulin (Cytoskeleton Inc., Denver, CO) at 37 °C for 20 min in BRB80 supplemented with 1 mM GTP and then diluted 1:10 in BRB80 + 20 µM taxol ...
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bioRxiv - Cell Biology 2021Quote: Actin Polymerization Biochem Kit (Cytoskeleton) was used to investigate F-actin depolymerization by cofilin in the absence or presence of control protein BSA ...
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bioRxiv - Cell Biology 2023Quote: ... rhodamine-labeled MTs were polymerized using a mixture containing 5% rhodamine-labeled tubulin (Cytoskeleton, TL590M), 5% biotin-labeled tubulin (Cytoskeleton ...
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bioRxiv - Biophysics 2022Quote: ... provided along with the kit (Cytoskeleton) was used to detect G- and F-actins ...
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bioRxiv - Molecular Biology 2022Quote: ... or 60min-old Tau:RNA or Tau:RNA:PEG coacervates were mixed with 5 μM porcine tubulin-Alexa594 (Cytoskeleton), and samples were pipetted onto the glass surface of an imaging dish (MaTek ...
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bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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bioRxiv - Cell Biology 2023Quote: ... which was 5% fluorescently labelled with HiLyte 488 or HiLyte 647 conjugated porcine brain tubulin (Cytoskeleton), was assembled at 40 μM at 1 mM GTP for 30 mins at 37°C ...
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bioRxiv - Cell Biology 2020Quote: A solution of 5 μg/mL Fibronectin labeled with HiLyte fluor 488 (FNR02-A, Cytoskeleton, Inc. USA) in PBS was incubated on glass bottom petri dish for 30 min at 4°C and rinsed with cold PBS thrice ...
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bioRxiv - Microbiology 2019Quote: ... 5 μL of the filtered supernatant was pipetted into a clear flat-bottom UV plate and 250 μL of 1x Advanced Protein Assay Reagent (Cytoskeleton Inc., CO) was added and mixed ...
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bioRxiv - Cancer Biology 2021Quote: Rac1 and RhoA GTPase actitation was measured by G-LISA Rac1 activation assay Kit and G-LISA RhoA activation Kit (Cytoskeleton), according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... assays were carried out using kits from Cytoskeleton, Inc ...
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bioRxiv - Cell Biology 2020Quote: Activated RhoA pulldown kits were purchased from Cytoskeleton. Sample lysates were prepared similar to the protocol used for G-LISAs ...
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bioRxiv - Physiology 2022Quote: ... the Cdc42 activation kit (Cytoskeleton Inc., Denver, USA) was used according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Imaging solution consisted of indicated concentration of porcine brain tubulin (5% or 15% rhodamine-labeled, Cytoskeleton Inc., Denver CO) and Alexa488-labeled tau in BRB80 supplemented with 1 mM GTP ...
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bioRxiv - Cell Biology 2022Quote: ... before incubating for 5 minutes at room temperature in 40 ng/µl rhodamine-labeled actin (Cat# AR05, Cytoskeleton, Inc.) freshly prepared in cytoskeletal buffer ...
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bioRxiv - Neuroscience 2022Quote: Rac1 activation was measured by using a Rac1 activation assay kit (Rac1 Pull-down Activation Assay Biochem Kit bead pulldown format, Cytoskeleton, catalog #BK035), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: Porcine Tubulin at 1:5 ratio (rhodamine-labeled: unlabeled) (cat. no. T240 and TL590M-A respectively, Cytoskeleton, Inc., Denver, USA) was reconstituted to 4.0 mg/mL in cold polymerization buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM guanidine carbonate and 1.2 mM ATP (from Actin Polymerization Buffer, cat. BSA02, and ATP, cat. BSA04, Cytoskeleton Inc.). Samples were incubated at 37 °C for 2 h.
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bioRxiv - Cancer Biology 2022Quote: RAS Activation Assay Biochem Kit™ (BK008; Cytoskeleton, Inc) was used to assess KRAS activity following manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... the microtubules were labeled by SiR700-tubulin Kit (Cytoskeleton) at a final concentration of 800 nM in cell culture medium for 2 h before imaging.
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Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavHbioRxiv - Molecular Biology 2023Quote: ... Actin polymerization biochem kit (BK003) was purchased from Cytoskeleton Inc ...
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bioRxiv - Cancer Biology 2022Quote: ... the RAS-GTP pull-down assay kit (Cytoskeleton #BK008) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... G-LISA RhoA or Rac1 activation kits from Cytoskeleton were used according to the manufacturer’s protocol.
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bioRxiv - Biophysics 2019Quote: ... This is done by mixing 5 μL sonicated GMPCPP microtubule (1 mg/mL short microtubules prepared from 97% pure tubulin (HTS03-A, Cytoskeleton, Inc), stored at −80 °C and thawed right before use ...
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bioRxiv - Molecular Biology 2022Quote: ... or 60 min-old Tau:polyA or Tau:PEG:polyA condensates containing 10% Cy3-labeled polyA were imaged prior and after the addition of 5 μM tubulin (bovine cycled tubulin, PurSolutions-PUR-032005) containing 10% HiLyte647-labeled tubulin (porcine tubulin, Cytoskeleton-TL670M). For tubulin bundle formation ...