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Citations for Cytoskeleton :
51 - 100 of 108 citations for Bone Gamma Carboxyglutamate Protein BGLAP Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... and Tyr-phosphorylated proteins were collected using Anti-phosphotyrosine Affinity Beads (Cytoskeleton, USA). Proteins were concentrated using the TCA/acetone precipitation method (Fic et al. ...
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bioRxiv - Biochemistry 2024Quote: ... we used a MT binding protein spin-down assay kit (# BK029, Cytoskeleton, Inc.) according to the manufacturer’s instructions ...
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Gα13 loss promotes tumor progression in the KPC transgenic mouse model of advanced pancreatic cancerbioRxiv - Cancer Biology 2021Quote: ... and the protein concentration was determined using Precision Red solution (Cytoskeleton, Inc., Denver, CO) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... MT assays were performed using the MT Binding Protein Spin-down kit from Cytoskeleton, Inc ...
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bioRxiv - Cell Biology 2023Quote: Actin binding and polymerization were assessed using the actin binding protein kit (Cytoskeleton, BK013). Actin pellet gel bands were quantified by densitometry using FIJI.
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bioRxiv - Pathology 2023Quote: ... and the protein concentration was determined using Precision Red solution (Cytoskeleton, Inc., Denver, CO). Equal amounts of protein were separated with a 10% SDS-PAGE electrophoresis gel ...
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal Mouse anti-RhoA antibody (Cytoskeleton inc. Cat#ARH05) was used at 1:500 dilution in TBST overnight.
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bioRxiv - Cell Biology 2020Quote: ... Monoclonal Mouse anti-RhoA antibody (Cytoskeleton inc. Cat#ARH05) was obtained through the Cytoskeleton inc Rho A activation Assay Biochem Kit and used at 1:500 ...
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bioRxiv - Immunology 2022Quote: ... Monoclonal Rac1 antibodies (Cat. # ARC03) were purchased from Cytoskeleton.
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bioRxiv - Immunology 2022Quote: Monoclonal Rac1 antibodies (Cat. # ARC03) were purchased from Cytoskeleton.
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bioRxiv - Plant Biology 2020Quote: ... 13 nM Arp2/3 protein complex and 15 nMWASp-VCA domain-GST purified (Cytoskeleton, USA) along with freshly purified ArF-BAR protein in concentrations of 200 ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal protein levels were confirmed by a control western blot of rabbit anti-Kinesin (Cytoskeleton) from the cleared lysates ...
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bioRxiv - Cell Biology 2022Quote: The Microtubule Binding Protein Spin-down assay was performed according to the manufacturers’ protocol (Cytoskeleton). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... Pyrene-labeled actin was incubated with recombinant ARP2/3 protein complex alone (Cat# RP01P, Cytoskeleton) or along with the human recombinant proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolation of ubiquitinated proteins was performed with the Signal-Seeker Ubiquitin Enrichment kit (Cytoskeleton). Cell fractionation was performed with the Qproteome Cell Compartment kit (Qiagen).
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bioRxiv - Molecular Biology 2019Quote: Mouse antibodies against Myc-tag (M185-3L/M047-3, MBL, Nagoya, Japan), rabbit antibody against GFP-tag (D110008, BBI, Shanghai, China) and sheep antibody against Tubulin (ATN02, Cytoskeleton, Denver, United States) were subjected to Western blot according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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bioRxiv - Neuroscience 2023Quote: ... or Cdc42 activation in vivo assay biochemical kits for the respective protein (Cytoskeleton Inc., Denver, CO). The tissues were lysed in lysis buffer (50mM Tris-HCl pH7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred to a clean tube and quantified using PrecisionRed (Cytoskeleton; ADV02-A) at OD600.
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bioRxiv - Biochemistry 2024Quote: ... The cells were lysed and protein lysates were used for G-LISA analysis per manufacturer’s (Cytoskeleton) instructions ...
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bioRxiv - Plant Biology 2020Quote: The actin polymerization modulation activity of the proteins was checked using Actin Polymerization Biochem Kit (Cytoskeleton, USA), using the manufacturer’s instruction ...
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bioRxiv - Biophysics 2024Quote: ... GST-tagged WCA domain of human WASP protein (GST-WCA) was purchased from Cytoskeleton (Cat. #VCG03-A) and used without further purification ...
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bioRxiv - Cell Biology 2020Quote: Primary antibodies used were rabbit anti-actin polyclonal (Cytoskeleton, Denver, CO) and mouse anti–MYO-3 monoclonal (5–6 ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with a polyclonal antibody against actin (1:500; Cytoskeleton Inc.) and developed by chemiluminescence ...
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal mouse anti-Cdc42 monoclonal antibody (1:250, Cytoskeleton, Cat. # ACD03), anti-Rac1 (1:250 ...
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bioRxiv - Microbiology 2020Quote: The microtubules binding experiments were performed with the Microtubule Binding Protein Spin-Down Assay Biochem Kit (Cytoskeleton, Inc.) following manufacturer protocol.
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bioRxiv - Neuroscience 2023Quote: Actin bundling assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem Kit (Cytoskeleton Inc.) according to protocol of the manufacturer.
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bioRxiv - Cell Biology 2021Quote: ... and incubation in secondary antibody and Acti-Stain 670 Phalloidin (Cytoskeleton, inc), plus Hoechst (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... moderate amounts of anti-ac-K antibody agarose beads (Cytoskeleton, Inc., USA) were added to the lysate and incubated for 12 h at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... 300µg of total cell protein was used to pull-down GTP-bound RAS/RAF-RBD complexes according to the manufacturer’s instructions (Cytoskeleton). Activated RAS or 20µg of total cell protein were separated using NuPAGE Novex Bis-Tris gels (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: F-actin disruption assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem kit (Cytoskeleton, USA) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were spun at maximum speed for 10mins before protein level was measured using Precision Red (Cytoskeleton, Inc., #ADV02). Samples were run using precast 4-12% NuPAGE Bis-Tris Acrylamide Gels (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies used were as follows: mouse anti-RAC1 (Cytoskeleton #ARC03, 1:250); mouse anti-RHOA (Cytoskeleton #ARH05 ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used: rabbit anti-cofilin (Cytoskeleton ACFL02; 1:500), rabbit anti-Ser3-phosphorylated cofilin (Cell Signaling Technology #3311 ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies used were mouse anti-Rac1 (1:500, Cytoskeleton Inc, #ARC03) and rabbit anti-GAPDH (1:3000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... In vitro microtubule-binding assay was performed using the microtubule-binding protein spin down assay kit (Cytoskeleton, Denver, CO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... F-actin and G-actin protein samples were collected according to the manufacturer’s instructions (#BK037, Cytoskeleton Inc., Denver, CO, USA). F-actin and G-actin were quantified by SDS-PAGE and western blot using actin antibody (#BK037 ...
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bioRxiv - Cell Biology 2020Quote: Muscle homogenates were prepared in buffer A as described above and an equal amount of protein from each sample was incubated with 20 µL of Rhotekin-RBD beads (Cytoskeleton) for 2 hours at 4°C and washed 4 times in ice-cold buffer containing 50 mM Tris (pH 7.4) ...
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bioRxiv - Cell Biology 2022Quote: ... and Rac or Rho activity was assessed in duplicate using the G-LISA small G-protein activation assay kit (BK128, BK124, Cytoskeleton) according to the manufacturer’s directions ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected upon 15min of centrifugation at >15000 rpm at 4°C and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). Equal amounts of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used included anti-β-actin (1:1,000, AAN01, Cytoskeleton, Inc., Denver, CO, USA), anti-AMPKα (1:1,000 ...
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bioRxiv - Microbiology 2019Quote: ... 5 μL of the filtered supernatant was pipetted into a clear flat-bottom UV plate and 250 μL of 1x Advanced Protein Assay Reagent (Cytoskeleton Inc., CO) was added and mixed ...
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bioRxiv - Biochemistry 2022Quote: ... The integrated signal measured from the area of the dynein bands is extracted using ImageJ and calibrated using known masses of purified dynein protein (CS-DN01, Cytoskeleton, Denver, CO, USA) that serve as standards ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated with monoclonal mouse anti-human RhoA primary antibody (# GL01A; 1:250; Cytoskeleton Inc.), followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02 ...
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bioRxiv - Cell Biology 2019Quote: ... followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02; 1:62.5; Cytoskeleton Inc.) on a shaker at 300 rpm at room temperature ...
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bioRxiv - Cell Biology 2020Quote: We used the following monoclonal antibodies: mouse anti-ubiquitin clone P4D1 (Cytoskeleton, Inc, Denver, Co, USA), mouse anti-β-actin clone BA3R (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was probed using the iBind flex kit and a primary anti-RhoA antibody (Cytoskeleton) at 1:200 and HRP-conjugated secondary (1:4000) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were analyzed by western blot using anti-ubiquitin antibody (P4D1, Cytoskeleton AUB01-HRP, 1:1000) and visualized with Clarity Western ECL Substrate (BioRad).
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bioRxiv - Cell Biology 2021Quote: ... MT affinity/dead-head spin was performed by binding motor protein to newly polymerized MTs (unlabeled, 2uM Paclitaxel (Taxol (Cytoskeleton, Denver, CO, US, TXD01) -stabilized ...