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Citations for Cytoskeleton :
1 - 50 of 218 citations for 7 methoxy 4 4 dimethylisochroman 1 3 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... and ∼4% rhodamine-tubulin (Cytoskeleton) were incubated with 1 mM GMPCPP or 1 mM GTP with 10 mM taxol at 37°C for 3 h ...
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bioRxiv - Microbiology 2024Quote: ... 4 μg of biotinylated tubulin (Cytoskeleton) and 100 μg of unlabelled pig tubulin in BRB80 buffer (80 mM PIPES pH 6.8 ...
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bioRxiv - Physiology 2021Quote: ... podocytes were fixed in 4% paraformaldehyde and 4% sucrose and stained with Acti-stain™ 488 Phalloidin (Cytoskeleton) and 50 cells per condition were assessed ...
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bioRxiv - Biophysics 2023Quote: ... or α4A/β2A tubulins ∼4% biotin-tubulin (Cytoskeleton) and ∼4% rhodamine-tubulin (Cytoskeleton ...
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bioRxiv - Molecular Biology 2023Quote: ... SiR-Tubulin incubation was performed for 4 h (Cytoskeleton, with 2 μM SiR-Tubulin and 10 μM verapamil) ...
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bioRxiv - Neuroscience 2020Quote: ... and secondary antibody at 1 h (4 °C) facilitated by an automated western blot liquid handler (Cytoskeleton GOBlot). Antibodies utilised included anti-eIF2α (abcam ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were fixed in PFA (4% PFA in Cytoskeleton Buffer (CB) – in mM ...
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bioRxiv - Cell Biology 2020Quote: ... Actin (2µM; 7% pyrene-labeled) was then polymerized in the presence of 20nM bovine Arp2/3 complex (Cytoskeleton Inc.) plus MBP-tagged fusion proteins in control buffer supplemented with 0.2mM ATP and 0.5mM DTT ...
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bioRxiv - Microbiology 2024Quote: Microtubules were made by mixing 4 μg of HiLyte Fluor 488 tubulin (Cytoskeleton), 4 μg of biotinylated tubulin (Cytoskeleton ...
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bioRxiv - Microbiology 2023Quote: ... IP of total SUMOylated protein fractions used either SUMO-1 or SUMO-2/3 Ab included with the Signal Seeker SUMOylation 1 or 2/3 Detection Kit (Cytoskeleton) and IPs were carried out according to the manufacturer instructions ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: MTs were polymerized by incubating 5 µM tubulin (4% HyLite 647 tubulin (Cytoskeleton #TL670M), ∼1% biotin tubulin (Cytoskeleton #T333P) ...
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bioRxiv - Cell Biology 2024Quote: ... anti-SUMO2/3 (Asm23, Cytoskeleton, 1:200 dilution), and anti-PML (sc966 ...
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bioRxiv - Cell Biology 2022Quote: ... NRIP-C-ΔWD6/7 and NRIP-WD6/7) from bacteria were incubated with F-actin (AD99, Cytoskeleton) in the binding buffer (10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: In order to constitutively activate RhoA vessels at day 4 were perfused with Rho Activator II (Cytoskeleton) at a concentration of 2 µg/mL for four hours prior to use in permeability tests or fixation for immunocytochemistry ...
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bioRxiv - Microbiology 2020Quote: ... Samples were fixed in 4% (v/v) Paraformaldehyde Aqueous Solution (15710-s, EM Grade) in PEM (Cytoskeleton-preserving buffer PIPES-EGTA-Magnesium ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... actomyosin contractility of MECs stably expressing an inducible ROCK construct was induced overnight using 4-hydroxytamoxifen and the actin cortex of these cells was labelled with SiR-actin dye (Cytoskeleton) according to manufacturer’s instructions prior to laser ablation.
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected upon 15min of centrifugation at >15000 rpm at 4°C and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). Equal amounts of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... microtubule seeds containing 7% rhodamine-tubulin (Cytoskeleton Inc., TL590M-B,) were polymerised in BRB80 (80 mM PIPES pH 6.9 ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... microtubule seeds containing 7% rhodamine-tubulin (Cytoskeleton Inc., TL590M-B) were polymerized in BRB80 (80 mM PIPES pH 6.9 ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: Dynamic MT seed assays were performed by polymerizing GMPCPP stabilized Cy5-MT seeds from 4 µM HiLyte 647 tubulin (Cytoskeleton, #TL670M) in BD buffer + 0.5 mM GMPCPP at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: ... The COS-7 cells were labelled with PKmito RED (Nanjing Genvivo Biotech Co., Ltd. PKMR-1, CY-SC052)/PKmito DEEP RED (Cytoskeleton, CY-SC055) 15 minutes in DMEM ...
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bioRxiv - Bioengineering 2021Quote: ... Acti-stain 488 phalloidin (7/1000 from stock, Cytoskeleton PHDG1-A). A 50 μL droplet of primary antibody solution was added to the recessed loading well of each substrate ...
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bioRxiv - Biophysics 2022Quote: ... COS-7 cells were incubated with SiR-Tubulin (Cytoskeleton, CY-SC002) for ~20 mins before imaging without washing.
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bioRxiv - Cell Biology 2020Quote: ... Arp2/3 complex (Cytoskeleton, Inc. RP01P), and GST-WASP-VCA (Cytoskeleton ...
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bioRxiv - Cell Biology 2019Quote: ... 50 nM Arp2/3 (Cytoskeleton Inc.), 1 μM VCA (Cytoskeleton Inc. ...
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bioRxiv - Biophysics 2020Quote: ... 3 μM Rhodamine tubulin (TL590M, Cytoskeleton), and 3 mM GMPCPP (NU-405S ...
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bioRxiv - Cell Biology 2023Quote: ... 50 nM Arp2/3 (Cytoskeleton; RP01P), 1 μM VCA domain of WASP (Cytoskeleton ...
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bioRxiv - Microbiology 2020Quote: ... recombinant human cofilin-1 (CFL1 P23528) and arp2/3 complex (ARPC4 Q148J6) from bovine brain were all purchased from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-SUMO2/3 (#12F3, Cytoskeleton Inc), Guinea Pig anti-SUMO2/3 (1:300 ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse anti-SUMO2/3 (#12F3, Cytoskeleton Inc), and Guinea Pig anti-SUMO2/3 (1:300 ...
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bioRxiv - Bioengineering 2019Quote: HUVECs were seeded on the PEG islands and control SiO2 substrates for 3 h and then flooded with cell culture medium containing 1:1000 dilution of SiR-DNA (Cytoskeleton Inc). An additional hour was required for cells to uptake SiR-DNA to be trackable through SiR-DNA probe using Leica DMI6000 microscope ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... the samples were preincubated for either 3 h with Rho/Rac/Cdc42 Activator I (1 µg/ml, Catalog number CN04, Cytoskeleton Inc), 30 min with MEK inhibitor PD 98059 (50 µM ...
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bioRxiv - Cell Biology 2021Quote: ... Microtubule growth was initiated by flowing in 10.7 μM tubulin containing 7% Hilyte647–labeled tubulin (Cytoskeleton Inc.) together with 36 nM motor proteins in the reaction buffer (BRB80 with 1 mM GTP ...
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bioRxiv - Biophysics 2019Quote: ... Porcine Arp2/3 complex is purchased from Cytoskeleton and used with no further purification ...
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bioRxiv - Biophysics 2021Quote: ... and Arp2/3 complex were purchased from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2019Quote: ... Microtubule growth was then initiated by flowing in 20 μM tubulin containing 7% Hilyte647-labeled tubulin (Cytoskeleton Inc.) together with cell extracts in the reaction buffer [2.5 mM GTP ...
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bioRxiv - Molecular Biology 2020Quote: ... and 60 nM Arp2/3 protein complex (#RP01P, Cytoskeleton) were also added to the actin polymerization buffer ...
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bioRxiv - Cell Biology 2021Quote: ... followed by polymerization of GMPCPP-caps for 1.5 min [7 uM bovine brain tubulin (gift of R. Ohi, University of Michigan) with 11% HiLyte647-tubuiln (Cytoskeleton. Inc.) in BRB80 with 2.6 mM GMPCPP ...
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bioRxiv - Biophysics 2023Quote: ... cell clusters on Day 7 were incubated overnight with 100 nM Acti-stainTM 488 Phalloidin (Cat. #PHDG1, Cytoskeleton Inc., USA) and washed thrice with PBS ...
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bioRxiv - Bioengineering 2020Quote: ... or 3 μg/mL C3 transferase (Cytoskeleton, Inc, Denver, CO).
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bioRxiv - Cell Biology 2020Quote: ... 3 G-LISA™ Activation Assay (purchased from Cytoskeleton Inc.), according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... The purified Arp2/3 protein complex from porcine brain (Cytoskeleton) and GST-tagged VCA domain of human WASP protein (Cytoskeleton ...
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bioRxiv - Cell Biology 2022Quote: ... 20 nM bovine brain Arp2/3 complex (Cytoskeleton; cat#RP01P), and 20 nM GST-WASP were mixed with purified Fbp17SH3 ...
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bioRxiv - Cell Biology 2022Quote: ... Cdc42 and ARP3 proteins or ARP2/3 protein complex (Cytoskeleton) were used as bait and full-length GST-ERK3 (SignalChem ...
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bioRxiv - Cell Biology 2021Quote: ... GDP-lattice microtubules were assembled in the chamber by polymerization of GTP-tubulin for 30 min [7 uM bovine brain tubulin (gift of R. Ohi, University of Michigan) with 6% HiLyte488-tubulin (Cytoskeleton Inc.) in BRB80 with GTP ...
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bioRxiv - Microbiology 2022Quote: ... around 3× 107 trophozoites were incubated in cell lysis buffer (Cytoskeleton Inc, Colorado, USA) with vigorous agitation at 4°C for 30 min and homogenized by a 23-gauge needle on a 5-ml syringe ...
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bioRxiv - Plant Biology 2020Quote: ... 13 nM Arp2/3 protein complex and 15 nMWASp-VCA domain-GST purified (Cytoskeleton, USA) along with freshly purified ArF-BAR protein in concentrations of 200 ...
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bioRxiv - Cell Biology 2022Quote: ... Pyrene-labeled actin was incubated with recombinant ARP2/3 protein complex alone (Cat# RP01P, Cytoskeleton) or along with the human recombinant proteins ...
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bioRxiv - Plant Biology 2019Quote: ... The seven protein subunits of the Arp2/3 protein complex were purified from bovine brain (Cytoskeleton). The GST-VCA fusion protein associated with the glutathione-agarose 4B (GE Healthcare ...