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Citations for Cytoskeleton :
1 - 50 of 201 citations for 6 6 dimethyl 1 phenyl 6 7 dihydro 1H indazol 4 5H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... 6 μM Biotin tubulin (T333P, Cytoskeleton), 3 μM Rhodamine tubulin (TL590M ...
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bioRxiv - Cell Biology 2021Quote: ... consisting of 6% biotinylated-tubulin (Cytoskeleton Inc) and 6% fluorescent (X-Rhodamine or HiLyte647 ...
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bioRxiv - Cell Biology 2021Quote: ... GDP-lattice microtubules were assembled in the chamber by polymerization of GTP-tubulin for 30 min [7 uM bovine brain tubulin (gift of R. Ohi, University of Michigan) with 6% HiLyte488-tubulin (Cytoskeleton Inc.) in BRB80 with GTP ...
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bioRxiv - Cell Biology 2021Quote: ... and 6% fluorescent (X-Rhodamine or HiLyte647) tubulin (Cytoskeleton Inc) in the presence of the nonhydrolyzable GTP analogue GMPCPP (Jena Bioscience ...
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bioRxiv - Cell Biology 2022Quote: ... NRIP-C-ΔWD6/7 and NRIP-WD6/7) from bacteria were incubated with F-actin (AD99, Cytoskeleton) in the binding buffer (10 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... microtubule seeds containing 7% rhodamine-tubulin (Cytoskeleton Inc., TL590M-B,) were polymerised in BRB80 (80 mM PIPES pH 6.9 ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... microtubule seeds containing 7% rhodamine-tubulin (Cytoskeleton Inc., TL590M-B) were polymerized in BRB80 (80 mM PIPES pH 6.9 ...
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bioRxiv - Bioengineering 2023Quote: ... The COS-7 cells were labelled with PKmito RED (Nanjing Genvivo Biotech Co., Ltd. PKMR-1, CY-SC052)/PKmito DEEP RED (Cytoskeleton, CY-SC055) 15 minutes in DMEM ...
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bioRxiv - Bioengineering 2021Quote: ... Acti-stain 488 phalloidin (7/1000 from stock, Cytoskeleton PHDG1-A). A 50 μL droplet of primary antibody solution was added to the recessed loading well of each substrate ...
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bioRxiv - Biophysics 2022Quote: ... COS-7 cells were incubated with SiR-Tubulin (Cytoskeleton, CY-SC002) for ~20 mins before imaging without washing.
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bioRxiv - Neuroscience 2020Quote: ... and secondary antibody at 1 h (4 °C) facilitated by an automated western blot liquid handler (Cytoskeleton GOBlot). Antibodies utilised included anti-eIF2α (abcam ...
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bioRxiv - Biophysics 2023Quote: ... and ∼4% rhodamine-tubulin (Cytoskeleton) were incubated with 1 mM GMPCPP or 1 mM GTP with 10 mM taxol at 37°C for 3 h ...
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bioRxiv - Cell Biology 2021Quote: ... Microtubule growth was initiated by flowing in 10.7 μM tubulin containing 7% Hilyte647–labeled tubulin (Cytoskeleton Inc.) together with 36 nM motor proteins in the reaction buffer (BRB80 with 1 mM GTP ...
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bioRxiv - Microbiology 2024Quote: ... 4 μg of biotinylated tubulin (Cytoskeleton) and 100 μg of unlabelled pig tubulin in BRB80 buffer (80 mM PIPES pH 6.8 ...
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bioRxiv - Cell Biology 2019Quote: ... Microtubule growth was then initiated by flowing in 20 μM tubulin containing 7% Hilyte647-labeled tubulin (Cytoskeleton Inc.) together with cell extracts in the reaction buffer [2.5 mM GTP ...
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bioRxiv - Physiology 2021Quote: ... podocytes were fixed in 4% paraformaldehyde and 4% sucrose and stained with Acti-stain™ 488 Phalloidin (Cytoskeleton) and 50 cells per condition were assessed ...
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bioRxiv - Cell Biology 2020Quote: ... Actin (2µM; 7% pyrene-labeled) was then polymerized in the presence of 20nM bovine Arp2/3 complex (Cytoskeleton Inc.) plus MBP-tagged fusion proteins in control buffer supplemented with 0.2mM ATP and 0.5mM DTT ...
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bioRxiv - Cell Biology 2021Quote: ... followed by polymerization of GMPCPP-caps for 1.5 min [7 uM bovine brain tubulin (gift of R. Ohi, University of Michigan) with 11% HiLyte647-tubuiln (Cytoskeleton. Inc.) in BRB80 with 2.6 mM GMPCPP ...
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bioRxiv - Biophysics 2023Quote: ... cell clusters on Day 7 were incubated overnight with 100 nM Acti-stainTM 488 Phalloidin (Cat. #PHDG1, Cytoskeleton Inc., USA) and washed thrice with PBS ...
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bioRxiv - Biophysics 2023Quote: ... or α4A/β2A tubulins ∼4% biotin-tubulin (Cytoskeleton) and ∼4% rhodamine-tubulin (Cytoskeleton ...
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bioRxiv - Molecular Biology 2023Quote: ... SiR-Tubulin incubation was performed for 4 h (Cytoskeleton, with 2 μM SiR-Tubulin and 10 μM verapamil) ...
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bioRxiv - Developmental Biology 2021Quote: ... cleaved proteins (quantified via a Bradford Assay) were incubated at room temperature for one hour with 0 or 5μM polymerized chicken F-actin (Cytoskeleton, Inc.). BSA was used a negative control ...
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bioRxiv - Developmental Biology 2020Quote: ... were microinjected into wild type embryos at one-cell stage together with 1.3 ng HiLyte Fluor™ 488-Tubulin (TL488M, Cytoskeleton, Inc.) or 1.3 ng TAMRA Rhodamine Tubulin (TL590M ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were fixed in PFA (4% PFA in Cytoskeleton Buffer (CB) – in mM ...
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bioRxiv - Microbiology 2024Quote: Microtubules were made by mixing 4 μg of HiLyte Fluor 488 tubulin (Cytoskeleton), 4 μg of biotinylated tubulin (Cytoskeleton ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: MTs were polymerized by incubating 5 µM tubulin (4% HyLite 647 tubulin (Cytoskeleton #TL670M), ∼1% biotin tubulin (Cytoskeleton #T333P) ...
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bioRxiv - Cell Biology 2020Quote: In order to constitutively activate RhoA vessels at day 4 were perfused with Rho Activator II (Cytoskeleton) at a concentration of 2 µg/mL for four hours prior to use in permeability tests or fixation for immunocytochemistry ...
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bioRxiv - Microbiology 2020Quote: ... Samples were fixed in 4% (v/v) Paraformaldehyde Aqueous Solution (15710-s, EM Grade) in PEM (Cytoskeleton-preserving buffer PIPES-EGTA-Magnesium ...
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bioRxiv - Neuroscience 2021Quote: ... p-ERK1/2 (CST, 9101, 1:1000), AKT (CST, 9272, 1:1000), p-AKT (CST, 9275, 1:1000) and Cofilin (Cytoskeleton, ACFL02, 1:500) were used to quantify protein levels ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... actomyosin contractility of MECs stably expressing an inducible ROCK construct was induced overnight using 4-hydroxytamoxifen and the actin cortex of these cells was labelled with SiR-actin dye (Cytoskeleton) according to manufacturer’s instructions prior to laser ablation.
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected upon 15min of centrifugation at >15000 rpm at 4°C and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). Equal amounts of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... and 1 μg ml-1 for Rho activator II (CNO3, Cytoskeleton). Cells were treated at least 2h at 37 °C and 5% CO2 ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: Dynamic MT seed assays were performed by polymerizing GMPCPP stabilized Cy5-MT seeds from 4 µM HiLyte 647 tubulin (Cytoskeleton, #TL670M) in BD buffer + 0.5 mM GMPCPP at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... MCAK (1:1000, Cytoskeleton), EB1 (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μM VCA (Cytoskeleton Inc.) and 250 nM rhodamine-actin were loaded onto flow chambers with or without 1mM GTP and subjected to TIRF imaging immediately ...
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bioRxiv - Cell Biology 2021Quote: ... SPY555-DNA (Cytoskeleton, 1:3,000) was used to label nuclear regions.
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bioRxiv - Microbiology 2020Quote: ... 1 μM SiR-tubulin (Cytoskeleton) was added to the medium 30 min prior to imaging ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... ∼1% biotin tubulin (Cytoskeleton #T333P), 95% unlabeled cycled bovine tubulin ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Rac1 (1:250, Cytoskeleton, Cat ...
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bioRxiv - Genetics 2019Quote: ... or DAPI (1:1,000, Cytoskeleton) at room temperature for 2 h ...
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bioRxiv - Cell Biology 2023Quote: ... or SirDNA (1 µM; Cytoskeleton) at 37°C at 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: ... SPY555-Actin (Cytoskeleton; 1:1,000), and NucSpot Live 650 (Biotium ...
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bioRxiv - Physiology 2020Quote: ... Ubiquitin (Cytoskeleton Inc., AUB01, 1:40). After washing four times with PBS ...
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bioRxiv - Physiology 2020Quote: ... Ubiquitin (Cytoskeleton Inc., AUB01, 1:400), CHIP/STUB1 (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 µM SiR-actin (Cytoskeleton) for 60 min ...
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bioRxiv - Systems Biology 2020Quote: 1: 2000 SiR-Actin (Cytoskeleton, Inc).
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bioRxiv - Developmental Biology 2023Quote: ... phalloidin labelling (Cytoskeleton, PHDR1; 1/500) or EdU labelling.
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bioRxiv - Cell Biology 2023Quote: ... rabbit anti-Khc (1:150, Cytoskeleton), rabbit anti-GFP (1:200 ...
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bioRxiv - Bioengineering 2023Quote: ... Rhodamine Phalloidin (1:500, Cytoskeleton, PHDR1), Alexa Fluor 488 Phalloidin (1:500 ...