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Citations for Cytoskeleton :
51 - 77 of 77 citations for 5 NITRO BENZO B THIOPHENE 2 CARBOXYLIC ACID METHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... which was 5% fluorescently labelled with HiLyte 488 or HiLyte 647 conjugated porcine brain tubulin (Cytoskeleton), was assembled at 40 μM at 1 mM GTP for 30 mins at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μM rabbit skeletal muscle actin (90% unlabeled and 10% rhodomine-actin) (Cytoskeleton Inc.), 1 μM Shi were incubated in 6.5 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... Cochlea were incubated for 2 minutes at RT in 0.5 µM TMR-actin (Cytoskeleton) diluted in labelling buffer (concentrations in mM) ...
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bioRxiv - Cell Biology 2020Quote: A solution of 5 μg/mL Fibronectin labeled with HiLyte fluor 488 (FNR02-A, Cytoskeleton, Inc. USA) in PBS was incubated on glass bottom petri dish for 30 min at 4°C and rinsed with cold PBS thrice ...
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bioRxiv - Biochemistry 2020Quote: ... provided as a lyophilized powder and resuspended at 2 mM anhydrous DMSO was purchased from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2022Quote: ... Microtubules were made by mixing 1 μl of HiLyte Fluor 488 tubulin (2 mg/ml, Cytoskeleton), 2 μl biotinylated tubulin (2 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: Microtubules were made by mixing 1.5 μL of 2 mg/mL HiLyte Fluor 488 tubulin (Cytoskeleton), 2 μL of 2 mg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Imaging solution consisted of indicated concentration of porcine brain tubulin (5% or 15% rhodamine-labeled, Cytoskeleton Inc., Denver CO) and Alexa488-labeled tau in BRB80 supplemented with 1 mM GTP ...
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bioRxiv - Cell Biology 2022Quote: ... before incubating for 5 minutes at room temperature in 40 ng/µl rhodamine-labeled actin (Cat# AR05, Cytoskeleton, Inc.) freshly prepared in cytoskeletal buffer ...
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bioRxiv - Cell Biology 2021Quote: Fluorescent MTs were polymerized at 2 mg/ml by incubating tubulin and rhodamine labeled tubulin (Cytoskeleton Inc.) at 10:1 ratio in BRB80 with 1 mM DTT and 1mM GTP for 5 min on ice ...
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bioRxiv - Biochemistry 2020Quote: Biotin-inactivated myosin was prepared by reacting 2 mg of rabbit skeletal muscle myosin (Cytoskeleton Cat # MYO2) with 5 μL of 250 mM EZ-Link-Maleimide-PEG11-Biotin dissolved in DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... 2 mM EGTA) supplemented with 4.51% of sucrose to adjust osmotic pressure (Cytoskeleton buffer with sucrose; CBS) and then fixed in 3% PFA in CBS for 15 min ...
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bioRxiv - Biochemistry 2021Quote: Porcine Tubulin at 1:5 ratio (rhodamine-labeled: unlabeled) (cat. no. T240 and TL590M-A respectively, Cytoskeleton, Inc., Denver, USA) was reconstituted to 4.0 mg/mL in cold polymerization buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM guanidine carbonate and 1.2 mM ATP (from Actin Polymerization Buffer, cat. BSA02, and ATP, cat. BSA04, Cytoskeleton Inc.). Samples were incubated at 37 °C for 2 h.
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bioRxiv - Microbiology 2023Quote: ... the samples were washed 2× with IF buffer and incubated with rhodamine phalloidin (PHDR1) (1:500) (Cytoskeleton, Inc.) for 30 minutes in the dark at 4 °C ...
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bioRxiv - Biophysics 2019Quote: ... This is done by mixing 5 μL sonicated GMPCPP microtubule (1 mg/mL short microtubules prepared from 97% pure tubulin (HTS03-A, Cytoskeleton, Inc), stored at −80 °C and thawed right before use ...
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bioRxiv - Molecular Biology 2022Quote: ... or 60 min-old Tau:polyA or Tau:PEG:polyA condensates containing 10% Cy3-labeled polyA were imaged prior and after the addition of 5 μM tubulin (bovine cycled tubulin, PurSolutions-PUR-032005) containing 10% HiLyte647-labeled tubulin (porcine tubulin, Cytoskeleton-TL670M). For tubulin bundle formation ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were then stimulated with vehicle or 100ng/ml EGF for 15 minutes or 2 hours and lysed with Cell Lysis Buffer (50mM Tris pH 7.5, 10mM MgCl2, 0.5M NaCl, 2% Igepal) (Cytoskeleton Inc.) containing protease and phoshatase inhibitors (Pierce/Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... 2% BSA) prior to immunostaining for 20 min with a 1:500 dilution of Acti-stain-555 (Cytoskeleton Inc). The ratio of cytoplasmic actin to cortical actin was measured using ImageJ (Fiji 2.1.0/1.53c ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed 2 times with 1xPBS and then incubated with rhodamine phalloidin (PHDR1; 1:500) (Cytoskeleton Inc.) in IF wash buffer for 30 min at RT in the dark ...
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bioRxiv - Microbiology 2022Quote: Proteins were collected in cell lysis buffer (50mM Tris pH 7.5, 10mM MgCl2, 0.5M NaCl, 2% Igepal) supplemented with 1X Protease inhibitor cocktail (Cytoskeleton, PIC02). Lysates were cleared by 1min centrifugation at 10,000g at 4°C and the collected supernatants were snap-frozen in liquid nitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... phenol-red free DMEM and incubated with MemGlow dyes (MemGlow 560, MG02-2 and MemGlow 590, MG03-10; Cytoskeleton) in serum-free ...
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bioRxiv - Neuroscience 2021Quote: ... p-ERK1/2 (CST, 9101, 1:1000), AKT (CST, 9272, 1:1000), p-AKT (CST, 9275, 1:1000) and Cofilin (Cytoskeleton, ACFL02, 1:500) were used to quantify protein levels ...
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bioRxiv - Cell Biology 2022Quote: ... embryos were washed three times with PBS and stained with 2 μg/mL Hoechst 33342 (Nacalai Tesque, 04929-82) and 20 nM Acti-stain™ 555 phalloidin (Cytoskeleton, PHDH1-A) in PBS containing 0.05% Triton X-100 at 4°C overnight in the dark ...
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bioRxiv - Biophysics 2021Quote: ... the cells were rinsed thoroughly and stained with 1:200 solution of secondary antibody in PBS for 2 hours followed by staining with Acti-Stain™ 670 Fluorescent Phalloidin (Cytoskeleton.Inc, catalog no. PHDN1). Then the cover-slides were mounted on glass slides using ProLong Diamond Antifade Mountant (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... Lipids accumulated in HLOs and cytoskeleton were stained with 2 μM BODIPY® 493/503 and 1 μM SiR-Actin (Cytoskeleton Inc., CO, USA), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended in DMEM-1%BSA and incubated in suspension for 30 min at 37°C in 10% CO2 and 2 μg/ml CT04 (#CT04-A, Cytoskeleton or 10 μM EHT1864 (#3872, Tocris). The vehicle control included the corresponding dilution of DMSO as needed ...