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Citations for Cytoskeleton :
151 - 197 of 197 citations for 2 Methoxy 2 3 4 5 Tetrabde Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... recombinant human cofilin-1 (CFL1 P23528) and arp2/3 complex (ARPC4 Q148J6) from bovine brain were all purchased from Cytoskeleton Inc ...
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bioRxiv - Molecular Biology 2022Quote: ... or 60min-old Tau:RNA or Tau:RNA:PEG coacervates were mixed with 5 μM porcine tubulin-Alexa594 (Cytoskeleton), and samples were pipetted onto the glass surface of an imaging dish (MaTek ...
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bioRxiv - Cell Biology 2023Quote: ... which was 5% fluorescently labelled with HiLyte 488 or HiLyte 647 conjugated porcine brain tubulin (Cytoskeleton), was assembled at 40 μM at 1 mM GTP for 30 mins at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... and secondary antibody at 1 h (4 °C) facilitated by an automated western blot liquid handler (Cytoskeleton GOBlot). Antibodies utilised included anti-eIF2α (abcam ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1 μg/ml RhoA inhibitor-I (Cytoskeleton, Inc., #CT-04), 2 μM FK-506 (Cayman No ...
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bioRxiv - Cell Biology 2023Quote: ... Rhosin (30 µM) and RhoCN03 (0.25 µg/ml) (Cytoskeleton, Inc.) were added on cells for 4 h prior to IFN-γ stimulation ...
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bioRxiv - Bioengineering 2019Quote: HUVECs were seeded on the PEG islands and control SiO2 substrates for 3 h and then flooded with cell culture medium containing 1:1000 dilution of SiR-DNA (Cytoskeleton Inc). An additional hour was required for cells to uptake SiR-DNA to be trackable through SiR-DNA probe using Leica DMI6000 microscope ...
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bioRxiv - Cell Biology 2021Quote: ... and 0.5 μl of 0.5 mg/ml biotin tubulin (#T333P, Cytoskeleton) in 15 µl MRB80 (80 mM PIPES pH 6.8 ...
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bioRxiv - Neuroscience 2019Quote: ... C3 exoenzyme from Clostridium Botulinum (0.5 µg/ml, Cytoskeleton Inc., #CT04) was infused in aCSF for 4 h before any combined treatment following instructions of the manufacturer.
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bioRxiv - Neuroscience 2019Quote: ... 1.0 µg/mL of RhoA inhibitor C3 transferase (Cytoskeleton, Cat# CT04) for 16 hours ...
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bioRxiv - Biophysics 2023Quote: ... and with 1.0 μg/ml of RhoA activator (Cytoskeleton, cat. CN03). Drugs led to decrease and increase of activities respectively (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... and 1 μg ml-1 for Rho activator II (CNO3, Cytoskeleton). Cells were treated at least 2h at 37 °C and 5% CO2 ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... Small GTPases (Rho/Rac/Cdc42) activator CN04 (Cytoskeleton, Inc.; 1μg/mL).
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... actomyosin contractility of MECs stably expressing an inducible ROCK construct was induced overnight using 4-hydroxytamoxifen and the actin cortex of these cells was labelled with SiR-actin dye (Cytoskeleton) according to manufacturer’s instructions prior to laser ablation.
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected upon 15min of centrifugation at >15000 rpm at 4°C and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). Equal amounts of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: Mouse antibodies against Myc-tag (M185-3L/M047-3, MBL, Nagoya, Japan), rabbit antibody against GFP-tag (D110008, BBI, Shanghai, China) and sheep antibody against Tubulin (ATN02, Cytoskeleton, Denver, United States) were subjected to Western blot according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The following inhibitors were used: C3 exoenzyme (1 mg/ml; Cytoskeleton, Inc), NSC23766 (50 mM ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: Dynamic MT seed assays were performed by polymerizing GMPCPP stabilized Cy5-MT seeds from 4 µM HiLyte 647 tubulin (Cytoskeleton, #TL670M) in BD buffer + 0.5 mM GMPCPP at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Imaging solution consisted of indicated concentration of porcine brain tubulin (5% or 15% rhodamine-labeled, Cytoskeleton Inc., Denver CO) and Alexa488-labeled tau in BRB80 supplemented with 1 mM GTP ...
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bioRxiv - Cell Biology 2022Quote: ... before incubating for 5 minutes at room temperature in 40 ng/µl rhodamine-labeled actin (Cat# AR05, Cytoskeleton, Inc.) freshly prepared in cytoskeletal buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM guanidine carbonate and 1.2 mM ATP (from Actin Polymerization Buffer, cat. BSA02, and ATP, cat. BSA04, Cytoskeleton Inc.). Samples were incubated at 37 °C for 2 h.
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bioRxiv - Cell Biology 2021Quote: ... Pharmacological treatments included 1 unit/ml Rho Activator I (CN01; Cytoskeleton, Denver, CO), 1 mM TAK165 (Mubritinib ...
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bioRxiv - Cell Biology 2020Quote: Microtubule polymerization assays were performed using 1.5 mg/mL purified tubulin (Cytoskeleton, Inc) that was taken from a 10 mg/mL flash frozen stock ...
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bioRxiv - Molecular Biology 2022Quote: ... or 60 min-old Tau:polyA or Tau:PEG:polyA condensates containing 10% Cy3-labeled polyA were imaged prior and after the addition of 5 μM tubulin (bovine cycled tubulin, PurSolutions-PUR-032005) containing 10% HiLyte647-labeled tubulin (porcine tubulin, Cytoskeleton-TL670M). For tubulin bundle formation ...
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bioRxiv - Biophysics 2022Quote: ... then coated with 6μg/ml of Rhodamine Fibronectin (Cat. # FNR01, CYTOSKELETON, Denver, CO, USA) by incubating at 37°C for 1hr ...
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bioRxiv - Cell Biology 2021Quote: ... Porcine brain microtubules were prepared from frozen aliquots of 5mg/ml tubulin (Cytoskeleton, Denver, CO) in polymerization buffer (BRB80 ...
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bioRxiv - Cell Biology 2023Quote: ... taxol-stabilised microtubules were prepared by mixing 2.5 ml of 100 μM porcine tubulin (Cytoskeleton) resuspended in BRB80 with 1 mM GTP (Cytoskeleton) ...
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bioRxiv - Cell Biology 2020Quote: ... and then placed in 1.5 ml imaging media containing 1 μM SiR-DNA (CY-SC007, Cytoskeleton) + 10 μM Verapamil (for nucleus staining ...
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bioRxiv - Biophysics 2023Quote: ... we performed additional tests: incubation with 1.0 μg/ml C3-transferase (RhoA inhibitor, Cytoskeleton, cat. CT04) and with 1.0 μg/ml of RhoA activator (Cytoskeleton ...
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bioRxiv - Biophysics 2021Quote: ... 0.2 mg ml−1 κ-casein and) lacking taxol but including 1 μM SiR-tubulin (Cytoskeleton inc.) and the mixture was allowed to bind to coverslips for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... 0.2 μl of 1 mg/ml HiLyte Fluor 647 or X-rhodamine-labelled tubulin (#TL670M, #TL6290M, Cytoskeleton) and 0.5 μl of 0.5 mg/ml biotin tubulin (#T333P ...
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bioRxiv - Biophysics 2021Quote: ... for 24 hours and treated with 5ng/ml TGFβ1 and 100nM SiR-actin kit (Cytoskeleton.Inc, CY-SC001).
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bioRxiv - Biophysics 2019Quote: ... the primary antibodies used in the study were sheep anti-tubulin (2.5 μg mL−1, ATN02, Cytoskeleton) and mouse anti-TOM20 (2.5 μg mL−1 ...
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bioRxiv - Biophysics 2023Quote: ... They are next incubated with a 10 μg/mL rhodamine-labelled fibronectin solution (Cytoskeleton, cat. FNR01-A) for 1h and then dried at room temperature for about 5min ...
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bioRxiv - Cell Biology 2023Quote: Stock actin (10 mg/ml) was diluted 1:10 into ATP-supplemented general actin buffer from Cytoskeleton Inc ...
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bioRxiv - Bioengineering 2023Quote: ... we incubated the glass coverslip with a 500 µg/mL solution of green fluorescent laminin (Cytoskeleton, Inc., LMN02) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Gels were then incubated with a 10 μg/ml laminin solution (1:1 rhodamine labeled (LMN01-A, Cytoskeleton, Inc.) to non-labeled laminin-1 (L2020 ...
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bioRxiv - Cell Biology 2019Quote: ... The PDMS stamps were inked either with 100 μg/ml 1:1 ratio of rhodamine-conjugated fibronectin (Cytoskeleton FNR01) and non-labelled fibronectin (Sigma-Aldrich F2006) ...
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bioRxiv - Molecular Biology 2021Quote: ... the pathway was first inhibited with fasudil 50µM (Bio-Techne, Minnesota, USA) or C3-exoenzyme 2µg/ml (Cytoskeleton, Denver, USA) for 15 hours and then activated with PDGF-AA at 50ng/ml for 20 minutes and proceed to the G-LISA or IF assay of myosin light chain phosphorylation (p-MLC) ...
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bioRxiv - Cell Biology 2019Quote: ... of each was incubated for 15 min at 37°C in General Tubulin Buffer with 2.25 mg/mL of 99% pure porcine tubulin and 1 mM GTP (all from Cytoskeleton Inc.), in a final volume of 50 μL ...
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bioRxiv - Cell Biology 2022Quote: ... the medium was replaced by a standard growth medium supplemented with 2% (vol/vol) GFR Matrigel and 10 µg/ml of a fluorescently labeled ECM molecule (HiLyte Fluor 488-labeled fibronectin, Cytoskeleton, Cat Number FNR02 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were cultured for nine days and fluorescent labeling of matrices for direct visualization during live cell microscopy was achieved by adding 1 µg/ml rhodamine-coupled fibronectin (Cytoskeleton) at day three ...
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bioRxiv - Biochemistry 2023Quote: ... was added to either supplemented actin buffer alone or in the presence of 0.4 mg mL-1 G-actin (cat. AKL99, Cytoskeleton Inc.). The final ligand concentration was 2.5 μM ...
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bioRxiv - Cell Biology 2023Quote: ... The coverslips were then rinsed in water and coated with 20 µg/mL fibronectin (1/10 Rhodamine Fibronectin (Cytoskeleton, FNR01), 9/10 fibronectin (FC010 ...
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bioRxiv - Biophysics 2022Quote: ... the microstamps were inked for 1 hour at room temperature with 25 μg/mL rhodamine FN solution (Cytoskeleton Inc., Colorado, United States). Deformable silicone Flexwell membranes (Flexcell inc. ...
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bioRxiv - Cell Biology 2020Quote: ... cells were grown at high density (150,000 cells/cm2) for 48 hours and were then treated with Rho-activator-II (Cytoskeleton: 1 µg/ml) for 3 hours ...