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Citations for Cytoskeleton :
301 - 350 of 374 citations for Human Nucleoredoxin like protein 1 NXNL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: G/F-actin assay was performed according to the manufacturer’s instructions (G/F-actin In Vivo Assay Kit, Cytoskeleton, Denver, CO). G-and F-actin bands on western blots were scanned by densitometry and the ratios of free G-actin to F-actin were calculated.
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bioRxiv - Microbiology 2022Quote: ... 110 μl of LAS2 buffer was prepared according to the protocol of a G-actin/F-actin In Vivo Assay Biochem Kit (Cytoskeleton, Inc.) and mixed with the pellet ...
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bioRxiv - Cell Biology 2023Quote: The F-actin/G-actin ratio was measured using G-Actin/F-Actin In Vivo Assay Biochem Kit (#BK037, Cytoskeleton, Inc.), following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: F- and G-actin fractions from lysates were prepared using a G-Actin/F-Actin in Vivo Assay Biochem kit (Cytoskeleton, #BK037) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... NIH3T3 cells were imaged using four wavelengths (405 nm for tagBFP; 488 nm for Citrine; 561 nm for mCherry or FusionRed; 674 nm for SiR700-Tubulin Kit, #CY-SC014, Cytoskeleton Inc.).
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bioRxiv - Cell Biology 2023Quote: Western blots to determine the ratio of G-actin to F-actin were performed on retinal lysates that were processed using a G-actin/F-actin In Vivo Assay Kit (Cytoskeleton, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Active GTP-bound GTPase levels in brain lysates were measured using G-LISA activation assay kits for Rac1 (Cytoskeleton, Inc., BK128) and RhoA (Cytoskeleton ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated for 30 min with 0.5 μM of SiR-actin or 60 min with 0.5 μM of SiR-tubulin and 10 μM of verapamil in culture medium (SiR Cytoskeleton Kit, Spirochrome SC006).
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bioRxiv - Neuroscience 2020Quote: RhoA activation was assessed with the G-LISA activation kit in accordance with the manufacturer’s instructions (Cytoskeleton Inc., catalog no. BK124-S). Active GTP-bound RhoA was detected with HRP-conjugated anti-RhoA antibodies ...
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bioRxiv - Cell Biology 2023Quote: Measurements of the microtubule-stimulated ATPase activities of KIF1A MD-NC-CC1-FHA and various mutants were performed by using the HTS Kinesin ATPase Endpoint Assay Biochem Kit (Cytoskeleton, Inc., BK053). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Total protein was extracted from multiple cell cultures (G411, G729, G797) using the RAC1 Activation Assay Biochem Kit per manufacturer’s instructions (Cytoskeleton Inc, #BK035-S) five days post transduction with lentiviral non-targeting or GJB2 shRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... The RhoActivity assay was performed using the RhoA pull-down activation assay biochem kit (Bead pull-down format) (Cytoskeleton, Cat No. BK036). The assay was carried out by incubating 800 µg of total cell lysate with 50 µg of Rhotekin as described in the manufacturers protocol following which western blots were performed ...
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bioRxiv - Cell Biology 2019Quote: ... Taxol-stabilized microtubules assembled from unlabeled and fluorescently-labeled porcine tubulin (10:1 ratio; Cytoskeleton) were introduced into the chamber ...
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bioRxiv - Biophysics 2021Quote: ... Washed cells were diluted 1:9 and stained with 50 nM SiR-actin (Spirochrome/Cytoskeleton) for 30 minutes (Lukinavicius et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Taxol-stabilized microtubules assembled from unlabeled and fluorescently-labeled porcine tubulin (10:1 ratio; Cytoskeleton) were introduced into the chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used included anti-β-actin (1:1,000, AAN01, Cytoskeleton, Inc., Denver, CO, USA), anti-AMPKα (1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with a 1:1000 dilution of SPY555-Actin Probe (CY-SC202, Cytoskeleton) in complete DMEM at 37°C for three hours prior to imaging ...
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bioRxiv - Biophysics 2023Quote: ... F-actin was labeled with rhodamine-phalloidin (PHDR1; Cytoskeleton, Inc., Denver, CO, USA; 1:167).
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bioRxiv - Bioengineering 2023Quote: ... with cell morphology and nucleus visualized using Acti-stain 555 phalloidin (1:2000, Cytoskeleton, PHDH1A) and DAPI (2ng/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Phalloidin (Alexa Fluor 488 and 670, Cytoskeleton Jomar, PHDG1-A, PHDN1-A, ICC, 1:500).
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bioRxiv - Developmental Biology 2024Quote: ... Cysts were incubated in a culture medium containing SPY650-actin (Cytoskeleton, Inc., 1:2000 dilution) for 3 h before indentation ...
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bioRxiv - Cancer Biology 2019Quote: The G-actin/F-actin assay was performed with a G-actin/F-actin in vivo assay kit (Cytoskeleton, Inc., Denver, CO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Active Rac levels were determined by pull-down assay using GST-PAK-CRIB immobilised to Glutathione beads according to manufacturer’s instructions (Cytoskeleton, Inc, Active Rac Kit). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: The G-actin/F-actin ratio in 229 R cells was assayed using the G-actin/F-actin In Vivo Assay Kit (Cytoskeleton, Denver, CO, USA). 229 R cells were scraped in LAS2 buffer and lysed ...
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bioRxiv - Cell Biology 2019Quote: Soluble G-actin was separated from filamentous F-actin using the In Vivo G-actin/F-actin Assay Kit (Cat# BK037, Cytoskeleton, Inc., Denver, CO) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... cells were rinsed with warmed PBS and cell lysates prepared in cell lysis buffer as instructed and provided by the RhoA or Rac1 pull-down instruction manual and kit (Cytoskeleton, Inc. Denver, CO). Protein quantitation was performed by BCA method and 600 μg of protein was incubated with 50 μg Rhotekin-RBD beads for RhoA pull-down or with 10 µg of PAK-PBD beads for Rac1 pull-down at 4°C for 1 h ...
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bioRxiv - Neuroscience 2023Quote: F-actin and G-actin content was measured using the G-actin/F-actin in vivo assay biochemical kit (Cytoskeleton Inc., Denver, CO) as previously described in (Laguesse et al. ...
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bioRxiv - Cell Biology 2020Quote: ... and then placed in 1.5 ml imaging media containing 1 μM SiR-DNA (CY-SC007, Cytoskeleton) + 10 μM Verapamil (for nucleus staining ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μm carboxylate beads (01-02-103, micromer) were coated with biotinylated laminin (LMN03, Cytoskeleton, Inc.) or FN7-10 (a four-domain segment of fibronectin responsible for cell binding and containing the RGD and PHSRN motifs102 ...
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bioRxiv - Biochemistry 2020Quote: ... Fixed tissues were washed in PBS (and stained with SiR-actin (1:1000 dilution, Cytoskeleton Inc.) for 3 hours at room temperature (RT ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained with 1 µM SiR-actin and 10 µM verapamil (Cytoskeleton, Inc., CY-SC001) for 1-3 hours before imaging ...
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bioRxiv - Microbiology 2020Quote: ... The cells were washed twice with PBS and incubated with rhodamine phalloidin (1:500) (Cytoskeleton Inc.) in IF washing buffer for 30 minutes at room temperature in the dark ...
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bioRxiv - Cell Biology 2019Quote: ... followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02; 1:62.5; Cytoskeleton Inc.) on a shaker at 300 rpm at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... 0.025% saponin and 1 mM ATP were added followed by 0.45 μM rhodamine actin (Cytoskeleton Inc). The culture medium was carefully removed by pipette and enough permeabilization buffer was added to cover cells ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were incubated in primary antibody diluted in TBST (1:500 anti-RHOA antibody, Cytoskeleton ARH04) or diluted in block (1:30,000 anti-a-alpha tubulin antibody ...
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bioRxiv - Cell Biology 2022Quote: ... Microtubules were made by mixing 1 μl of HiLyte Fluor 488 tubulin (2 mg/ml, Cytoskeleton), 2 μl biotinylated tubulin (2 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were analyzed by western blot using anti-ubiquitin antibody (P4D1, Cytoskeleton AUB01-HRP, 1:1000) and visualized with Clarity Western ECL Substrate (BioRad).
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... actin filaments (Acti-stain 670 phalloidin, 1:600 dilution; Cat: SKU PHDN1, Cytoskeleton, Denver, CO USA). Imaging was performed using a spinning disk confocal microscope (Yokogawa CSU-X1 ...
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bioRxiv - Biophysics 2020Quote: ... F-actin filaments and the nuclei were labeled with 1 µM of SiR-actin (Cytoskeleton, Denver, USA) and 1 µg/ml of Hoechst 33342 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoblots were performed using the following primary antibodies: rabbit anti-KIF15 (1:1000; Cytoskeleton Inc., Cat#AKIN13), rabbit anti-KIF2A (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... 0.2 μl of 1 mg/ml HiLyte Fluor 647 or X-rhodamine-labelled tubulin (#TL670M, #TL6290M, Cytoskeleton) and 0.5 μl of 0.5 mg/ml biotin tubulin (#T333P ...
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bioRxiv - Biophysics 2019Quote: ... the primary antibodies used in the study were sheep anti-tubulin (2.5 μg mL−1, ATN02, Cytoskeleton) and mouse anti-TOM20 (2.5 μg mL−1 ...
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bioRxiv - Cell Biology 2023Quote: Stock actin (10 mg/ml) was diluted 1:10 into ATP-supplemented general actin buffer from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were stored at −20°C prior to western blot analysis for actin (Cytoskeleton 1:1000). Standards (10 ...
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bioRxiv - Developmental Biology 2023Quote: ... The explants were then incubated overnight with a 1:100 dilution of Alexa488 Acti-stain (Cytoskeleton PHDG1) and imaged using a Nikon AX confocal microscope and a10x objective.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were stained in suspension with a 1:300 dilution of Anti-Stain 488 Fluorescent Phalloidin (Cytoskeleton, #PHDG1), gently mixed ...
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bioRxiv - Neuroscience 2020Quote: ... and secondary antibody at 1 h (4 °C) facilitated by an automated western blot liquid handler (Cytoskeleton GOBlot). Antibodies utilised included anti-eIF2α (abcam ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The blocked tissue samples were then transferred to Acti-stain ™ 670 Phalloidin (1:1200, Cytoskeleton Cat#: PHDN1) reagent diluted in PBT and normal donkey serum ...
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bioRxiv - Microbiology 2023Quote: ... the samples were washed 2× with IF buffer and incubated with rhodamine phalloidin (PHDR1) (1:500) (Cytoskeleton, Inc.) for 30 minutes in the dark at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: GMPCPP-stabilized microtubules were assembled using 50 μM unlabeled porcine tubulin containing 1:12 biotin-labeled porcine tubulin (Cytoskeleton) and polymerized in the presence of 1mM GMPCPP (Jena Bioscience ...