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Citations for Cytoskeleton :
201 - 237 of 237 citations for 6 4 Methoxy phenyl 3 phenyl pyrazolo 1 5 a pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02; 1:62.5; Cytoskeleton Inc.) on a shaker at 300 rpm at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... 0.025% saponin and 1 mM ATP were added followed by 0.45 μM rhodamine actin (Cytoskeleton Inc). The culture medium was carefully removed by pipette and enough permeabilization buffer was added to cover cells ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were incubated in primary antibody diluted in TBST (1:500 anti-RHOA antibody, Cytoskeleton ARH04) or diluted in block (1:30,000 anti-a-alpha tubulin antibody ...
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bioRxiv - Cell Biology 2022Quote: ... Microtubules were made by mixing 1 μl of HiLyte Fluor 488 tubulin (2 mg/ml, Cytoskeleton), 2 μl biotinylated tubulin (2 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were analyzed by western blot using anti-ubiquitin antibody (P4D1, Cytoskeleton AUB01-HRP, 1:1000) and visualized with Clarity Western ECL Substrate (BioRad).
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... actin filaments (Acti-stain 670 phalloidin, 1:600 dilution; Cat: SKU PHDN1, Cytoskeleton, Denver, CO USA). Imaging was performed using a spinning disk confocal microscope (Yokogawa CSU-X1 ...
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bioRxiv - Biophysics 2020Quote: ... F-actin filaments and the nuclei were labeled with 1 µM of SiR-actin (Cytoskeleton, Denver, USA) and 1 µg/ml of Hoechst 33342 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoblots were performed using the following primary antibodies: rabbit anti-KIF15 (1:1000; Cytoskeleton Inc., Cat#AKIN13), rabbit anti-KIF2A (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... 0.2 μl of 1 mg/ml HiLyte Fluor 647 or X-rhodamine-labelled tubulin (#TL670M, #TL6290M, Cytoskeleton) and 0.5 μl of 0.5 mg/ml biotin tubulin (#T333P ...
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bioRxiv - Biophysics 2019Quote: ... the primary antibodies used in the study were sheep anti-tubulin (2.5 μg mL−1, ATN02, Cytoskeleton) and mouse anti-TOM20 (2.5 μg mL−1 ...
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bioRxiv - Cell Biology 2023Quote: Stock actin (10 mg/ml) was diluted 1:10 into ATP-supplemented general actin buffer from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were stored at −20°C prior to western blot analysis for actin (Cytoskeleton 1:1000). Standards (10 ...
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bioRxiv - Developmental Biology 2023Quote: ... The explants were then incubated overnight with a 1:100 dilution of Alexa488 Acti-stain (Cytoskeleton PHDG1) and imaged using a Nikon AX confocal microscope and a10x objective.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were stained in suspension with a 1:300 dilution of Anti-Stain 488 Fluorescent Phalloidin (Cytoskeleton, #PHDG1), gently mixed ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The blocked tissue samples were then transferred to Acti-stain ™ 670 Phalloidin (1:1200, Cytoskeleton Cat#: PHDN1) reagent diluted in PBT and normal donkey serum ...
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bioRxiv - Microbiology 2023Quote: ... the samples were washed 2× with IF buffer and incubated with rhodamine phalloidin (PHDR1) (1:500) (Cytoskeleton, Inc.) for 30 minutes in the dark at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: GMPCPP-stabilized microtubules were assembled using 50 μM unlabeled porcine tubulin containing 1:12 biotin-labeled porcine tubulin (Cytoskeleton) and polymerized in the presence of 1mM GMPCPP (Jena Bioscience ...
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bioRxiv - Cell Biology 2021Quote: ... Seeds were formed by shearing larger MTs (GMPCPP-stabilized, labeled 1:40 with HiLyte 488 tubulin (Cytoskeleton, TL488M-B) with a Hamilton syringe (Avanti Polar Lipids ...
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bioRxiv - Microbiology 2022Quote: ... 2% BSA) prior to immunostaining for 20 min with a 1:500 dilution of Acti-stain-555 (Cytoskeleton Inc). The ratio of cytoplasmic actin to cortical actin was measured using ImageJ (Fiji 2.1.0/1.53c ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed 2 times with 1xPBS and then incubated with rhodamine phalloidin (PHDR1; 1:500) (Cytoskeleton Inc.) in IF wash buffer for 30 min at RT in the dark ...
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bioRxiv - Cell Biology 2019Quote: ... of each was incubated for 15 min at 37°C in General Tubulin Buffer with 2.25 mg/mL of 99% pure porcine tubulin and 1 mM GTP (all from Cytoskeleton Inc.), in a final volume of 50 μL ...
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bioRxiv - Cell Biology 2021Quote: ... Tubulin was labeled in 1:10 proportions with biotin or X-rhodamine according to published protocols or mixed with prelabeled tubulin purchased from Cytoskeleton, Inc.
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bioRxiv - Cell Biology 2022Quote: ... Cells were cultured for nine days and fluorescent labeling of matrices for direct visualization during live cell microscopy was achieved by adding 1 µg/ml rhodamine-coupled fibronectin (Cytoskeleton) at day three ...
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bioRxiv - Biochemistry 2023Quote: ... was added to either supplemented actin buffer alone or in the presence of 0.4 mg mL-1 G-actin (cat. AKL99, Cytoskeleton Inc.). The final ligand concentration was 2.5 μM ...
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bioRxiv - Cell Biology 2023Quote: ... The isolated muscles were then incubated in PBS with 1:1000 488-Phalloidin or 555-Phalloidin (Acti-stain, Cytoskeleton Inc.) to stain the actin filaments ...
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bioRxiv - Cell Biology 2023Quote: ... The coverslips were then rinsed in water and coated with 20 µg/mL fibronectin (1/10 Rhodamine Fibronectin (Cytoskeleton, FNR01), 9/10 fibronectin (FC010 ...
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bioRxiv - Neuroscience 2020Quote: ... Starved flies were then moved to tubes with standard corn meal and sugar cane syrup supplemented with taxol (10 nM, 100 nM, 1 µM or 10 µM, Paclitaxel Cytoskeleton Inc.) or DMSO (0.01% ...
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bioRxiv - Cell Biology 2021Quote: ... Chromosomes were labeled in the inducible DHC-KO cell line (Figures 1C, 1I, 1J) by incubating cells in 1 µM SiR-DNA and 10 µM verapamil (CY-SC007, Cytoskeleton Inc.) for 60 min prior to imaging ...
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bioRxiv - Biophysics 2022Quote: ... the microstamps were inked for 1 hour at room temperature with 25 μg/mL rhodamine FN solution (Cytoskeleton Inc., Colorado, United States). Deformable silicone Flexwell membranes (Flexcell inc. ...
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bioRxiv - Neuroscience 2021Quote: ... washed and the immunoprecipitated resolved on 12% SDS-PAGE and detected via Western blot analysis using Cdc42 (mouse anti-Cdc42, ACD03 Cytoskeleton; 1:250), Rac1 (mouse anti-Rac1 ...
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bioRxiv - Neuroscience 2020Quote: ... probed with the rabbit anti-myosin 18A polyclonal antibody directed against the C-terminal 18 residues of myosin 18Aα/β (1:1000, cite JB Cytoskeleton 2017) and with a mouse anti-RACK1 monoclonal antibody (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were grown at high density (150,000 cells/cm2) for 48 hours and were then treated with Rho-activator-II (Cytoskeleton: 1 µg/ml) for 3 hours ...
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bioRxiv - Bioengineering 2023Quote: ... The COS-7 cells were labelled with PKmito RED (Nanjing Genvivo Biotech Co., Ltd. PKMR-1, CY-SC052)/PKmito DEEP RED (Cytoskeleton, CY-SC055) 15 minutes in DMEM ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were resolved on 12% SDS-PAGE and detected via Western blot analysis using actin (rabbit anti-actin, BK037 Cytoskeleton Inc.; 1:500) and visualized by ECL (Pierce ...
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bioRxiv - Cell Biology 2019Quote: ... HCLE cells were pre-loaded with either CellMask™ Deep Red Plasma membrane stain as described above or 1 μM of SiR-Actin Spirochrome probe (Cytoskeleton Inc., Denver, CO) for 10 minutes at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: ... Lipids accumulated in HLOs and cytoskeleton were stained with 2 μM BODIPY® 493/503 and 1 μM SiR-Actin (Cytoskeleton Inc., CO, USA), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... GMPCPP-stabilised microtubules were polymerised by resuspending 1 μl aliquots of HiLyte-647 labelled tubulin mixes (60% unlabelled tubulin, 20% HiLyte-647 tubulin, 20% biotinylated tubulin, Cytoskeleton, Inc. T240, TL670M, T333, respectively) in 50 μl of warm (80 mM PIPES ...