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Citations for Cytoskeleton :
151 - 200 of 254 citations for Human G Protein Coupled Receptor Family C Group 6 Member A GPRC6A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... and then incubated with monoclonal mouse anti-human RhoA primary antibody (# GL01A; 1:250; Cytoskeleton Inc.), followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02 ...
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bioRxiv - Cancer Biology 2021Quote: ... F-actin and G-actin were quantified by SDS-PAGE and western blot using actin antibody (#BK037, Cytoskeleton Inc.).
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bioRxiv - Biophysics 2021Quote: The following cytoskeleton proteins are purchased: porcine brain tubulin (Cytoskeleton T240), HiLyte Fluor-488-labeled tubulin (Cytoskeleton TL488M-A) ...
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bioRxiv - Cell Biology 2020Quote: ... reserving a small amount for protein quantification using Precision Red (Cytoskeleton). Prior to measuring GTPase activity ...
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bioRxiv - Cell Biology 2021Quote: Soluble tubulin polymerization mix containing 50 μM tubulin protein (Cytoskeleton, T240), 1 μM TRITC-labeled tubulin protein (Cytoskeleton ...
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bioRxiv - Cell Biology 2021Quote: ... 22 μl of 5 mg ml−1 tubulin protein (Cytoskeleton, T240) was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton ...
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bioRxiv - Biochemistry 2023Quote: ... was added to either supplemented actin buffer alone or in the presence of 0.4 mg mL-1 G-actin (cat. AKL99, Cytoskeleton Inc.). The final ligand concentration was 2.5 μM ...
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bioRxiv - Cell Biology 2021Quote: ... GDP-lattice microtubules were assembled in the chamber by polymerization of GTP-tubulin for 30 min [7 uM bovine brain tubulin (gift of R. Ohi, University of Michigan) with 6% HiLyte488-tubulin (Cytoskeleton Inc.) in BRB80 with GTP ...
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bioRxiv - Genetics 2024Quote: ... Protein concentration was determined using APA assay (Cytoskeleton Inc. Denver, CO, USA) and 50 μg total protein were separated on SDS-PAGE followed by western blot ...
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bioRxiv - Biochemistry 2023Quote: ... and protein concentrations were estimated using the Precision Red reagent (Cytoskeleton Inc). Protein samples were prepared by mixing 1X LDS (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... A mix of 20 μM of porcine brain tubulin protein (Cytoskeleton/Tebu-Bio) with 1 mM GMPCPP (Jena Bioscience ...
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bioRxiv - Biophysics 2020Quote: 0.4 µM Myosin Motor protein S1 Fragment (Rabbit Skeletal Muscle, Cytoskeleton #CS-MYS04) was incubated either alone or with 2 µM UCS proteins for 10 minutes either at 42°C or on ice in Mg-buffer (10mM Hepes-KOH ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... and Tyr-phosphorylated proteins were collected using Anti-phosphotyrosine Affinity Beads (Cytoskeleton, USA). Proteins were concentrated using the TCA/acetone precipitation method (Fic et al. ...
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bioRxiv - Cell Biology 2022Quote: ... NRIP-C-ΔWD6/7 and NRIP-WD6/7) from bacteria were incubated with F-actin (AD99, Cytoskeleton) in the binding buffer (10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were stored at −20°C prior to western blot analysis for actin (Cytoskeleton 1:1000). Standards (10 ...
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bioRxiv - Microbiology 2020Quote: ... recombinant human cofilin-1 (CFL1 P23528) and arp2/3 complex (ARPC4 Q148J6) from bovine brain were all purchased from Cytoskeleton Inc ...
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bioRxiv - Biophysics 2020Quote: The data for the linear actin filaments was taken from 18 so the preparation procedure is identical [as described therein]: 250 μg of rabbit skeletal muscle G-actin (AKL99-A; Cytoskeleton, Inc., Denver, CO) were resuspended to using 250 μL of General Actin Buffer (BSA01 ...
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Gα13 loss promotes tumor progression in the KPC transgenic mouse model of advanced pancreatic cancerbioRxiv - Cancer Biology 2021Quote: ... and the protein concentration was determined using Precision Red solution (Cytoskeleton, Inc., Denver, CO) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... and the protein concentration was determined using Precision Red solution (Cytoskeleton, Inc., Denver, CO). Equal amounts of protein were separated with a 10% SDS-PAGE electrophoresis gel ...
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bioRxiv - Neuroscience 2020Quote: ... and secondary antibody at 1 h (4 °C) facilitated by an automated western blot liquid handler (Cytoskeleton GOBlot). Antibodies utilised included anti-eIF2α (abcam ...
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bioRxiv - Cell Biology 2021Quote: Actin Polymerization Biochem Kit (Cytoskeleton) was used to investigate F-actin depolymerization by cofilin in the absence or presence of control protein BSA ...
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bioRxiv - Plant Biology 2020Quote: ... 13 nM Arp2/3 protein complex and 15 nMWASp-VCA domain-GST purified (Cytoskeleton, USA) along with freshly purified ArF-BAR protein in concentrations of 200 ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal protein levels were confirmed by a control western blot of rabbit anti-Kinesin (Cytoskeleton) from the cleared lysates ...
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bioRxiv - Cell Biology 2022Quote: The Microtubule Binding Protein Spin-down assay was performed according to the manufacturers’ protocol (Cytoskeleton). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... Pyrene-labeled actin was incubated with recombinant ARP2/3 protein complex alone (Cat# RP01P, Cytoskeleton) or along with the human recombinant proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... Rabbit polyclonal antibody recognizing the Kif11/Eg5 protein (Cat. #AKIN03, discontinued) was purchased from Cytoskeleton, Inc ...
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bioRxiv - Biophysics 2022Quote: ... provided along with the kit (Cytoskeleton) was used to detect G- and F-actins ...
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bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred to a clean tube and quantified using PrecisionRed (Cytoskeleton; ADV02-A) at OD600.
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bioRxiv - Neuroscience 2020Quote: ... probed with the rabbit anti-myosin 18A polyclonal antibody directed against the C-terminal 18 residues of myosin 18Aα/β (1:1000, cite JB Cytoskeleton 2017) and with a mouse anti-RACK1 monoclonal antibody (1:1000 ...
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bioRxiv - Biophysics 2021Quote: ... labeled tubulin with 9 μL unlabeled MTs and transferred to a 37°C water bath for 20 min and stabilized by adding 10 μM paclitaxel (Cytoskeleton, Denver, CO). MTs were stored at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... assays were carried out using kits from Cytoskeleton, Inc ...
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bioRxiv - Cell Biology 2020Quote: Activated RhoA pulldown kits were purchased from Cytoskeleton. Sample lysates were prepared similar to the protocol used for G-LISAs ...
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bioRxiv - Physiology 2022Quote: ... the Cdc42 activation kit (Cytoskeleton Inc., Denver, USA) was used according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... 300µg of total cell protein was used to pull-down GTP-bound RAS/RAF-RBD complexes according to the manufacturer’s instructions (Cytoskeleton). Activated RAS or 20µg of total cell protein were separated using NuPAGE Novex Bis-Tris gels (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were spun at maximum speed for 10mins before protein level was measured using Precision Red (Cytoskeleton, Inc., #ADV02). Samples were run using precast 4-12% NuPAGE Bis-Tris Acrylamide Gels (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: Rac1 activation was measured by using a Rac1 activation assay kit (Rac1 Pull-down Activation Assay Biochem Kit bead pulldown format, Cytoskeleton, catalog #BK035), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: RAS Activation Assay Biochem Kit™ (BK008; Cytoskeleton, Inc) was used to assess KRAS activity following manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... the microtubules were labeled by SiR700-tubulin Kit (Cytoskeleton) at a final concentration of 800 nM in cell culture medium for 2 h before imaging.
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Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavHbioRxiv - Molecular Biology 2023Quote: ... Actin polymerization biochem kit (BK003) was purchased from Cytoskeleton Inc ...
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bioRxiv - Cancer Biology 2022Quote: ... the RAS-GTP pull-down assay kit (Cytoskeleton #BK008) was used ...
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bioRxiv - Cell Biology 2020Quote: Muscle homogenates were prepared in buffer A as described above and an equal amount of protein from each sample was incubated with 20 µL of Rhotekin-RBD beads (Cytoskeleton) for 2 hours at 4°C and washed 4 times in ice-cold buffer containing 50 mM Tris (pH 7.4) ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended in DMEM-1%BSA and incubated in suspension for 30 min at 37°C in 10% CO2 and 2 μg/ml CT04 (#CT04-A, Cytoskeleton or 10 μM EHT1864 (#3872, Tocris). The vehicle control included the corresponding dilution of DMSO as needed ...
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bioRxiv - Microbiology 2023Quote: ... For ubiquitin pulldown assays using the SignalSeeker kit (Cytoskeleton Inc), cells were lysed in provided BlastR buffer with protease inhibitor and NEM ...
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bioRxiv - Genomics 2024Quote: ... and 500 nM SiR-tubulin Kit (Cytoskeleton, Denver, Colorado, U.S.) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... 5 μL of the filtered supernatant was pipetted into a clear flat-bottom UV plate and 250 μL of 1x Advanced Protein Assay Reagent (Cytoskeleton Inc., CO) was added and mixed ...
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bioRxiv - Developmental Biology 2019Quote: Bulk Rac1 activity was measured using an assay kit (BK035, Cytoskeleton), as previously described (Hara et al. ...
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bioRxiv - Biophysics 2022Quote: The RhoA Activation Assay Biochem Kit (bead pull-down format, Cytoskeleton) was used for performing the pull-down assay on 2D monolayers that were either uninduced or induced for the (DN ...
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bioRxiv - Cell Biology 2023Quote: RhoA GAP assays were performed using a kit provided by Cytoskeleton Inc (Denver Colorado Cat no ...
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bioRxiv - Biochemistry 2022Quote: ... The integrated signal measured from the area of the dynein bands is extracted using ImageJ and calibrated using known masses of purified dynein protein (CS-DN01, Cytoskeleton, Denver, CO, USA) that serve as standards ...