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Citations for Cytoskeleton :
101 - 150 of 251 citations for Mouse Two pore calcium channel protein 2 Tpcn2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... 13 nM Arp2/3 protein complex and 15 nMWASp-VCA domain-GST purified (Cytoskeleton, USA) along with freshly purified ArF-BAR protein in concentrations of 200 ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal protein levels were confirmed by a control western blot of rabbit anti-Kinesin (Cytoskeleton) from the cleared lysates ...
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bioRxiv - Cell Biology 2022Quote: The Microtubule Binding Protein Spin-down assay was performed according to the manufacturers’ protocol (Cytoskeleton). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... Pyrene-labeled actin was incubated with recombinant ARP2/3 protein complex alone (Cat# RP01P, Cytoskeleton) or along with the human recombinant proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... Rabbit polyclonal antibody recognizing the Kif11/Eg5 protein (Cat. #AKIN03, discontinued) was purchased from Cytoskeleton, Inc ...
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bioRxiv - Biophysics 2022Quote: ... provided along with the kit (Cytoskeleton) was used to detect G- and F-actins ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μM rabbit skeletal muscle actin (90% unlabeled and 10% rhodomine-actin) (Cytoskeleton Inc.), 1 μM Shi were incubated in 6.5 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... Cochlea were incubated for 2 minutes at RT in 0.5 µM TMR-actin (Cytoskeleton) diluted in labelling buffer (concentrations in mM) ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred to a clean tube and quantified using PrecisionRed (Cytoskeleton; ADV02-A) at OD600.
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bioRxiv - Biochemistry 2024Quote: ... The cells were lysed and protein lysates were used for G-LISA analysis per manufacturer’s (Cytoskeleton) instructions ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated with monoclonal mouse anti-human RhoA primary antibody (# GL01A; 1:250; Cytoskeleton Inc.), followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02 ...
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bioRxiv - Cell Biology 2019Quote: ... followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02; 1:62.5; Cytoskeleton Inc.) on a shaker at 300 rpm at room temperature ...
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bioRxiv - Cell Biology 2020Quote: We used the following monoclonal antibodies: mouse anti-ubiquitin clone P4D1 (Cytoskeleton, Inc, Denver, Co, USA), mouse anti-β-actin clone BA3R (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... GST-tagged WCA domain of human WASP protein (GST-WCA) was purchased from Cytoskeleton (Cat. #VCG03-A) and used without further purification ...
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bioRxiv - Biochemistry 2020Quote: ... provided as a lyophilized powder and resuspended at 2 mM anhydrous DMSO was purchased from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2022Quote: ... Microtubules were made by mixing 1 μl of HiLyte Fluor 488 tubulin (2 mg/ml, Cytoskeleton), 2 μl biotinylated tubulin (2 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: Microtubules were made by mixing 1.5 μL of 2 mg/mL HiLyte Fluor 488 tubulin (Cytoskeleton), 2 μL of 2 mg/mL ...
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bioRxiv - Cancer Biology 2021Quote: Rac1 and RhoA GTPase actitation was measured by G-LISA Rac1 activation assay Kit and G-LISA RhoA activation Kit (Cytoskeleton), according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... assays were carried out using kits from Cytoskeleton, Inc ...
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bioRxiv - Cell Biology 2020Quote: Activated RhoA pulldown kits were purchased from Cytoskeleton. Sample lysates were prepared similar to the protocol used for G-LISAs ...
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bioRxiv - Physiology 2022Quote: ... the Cdc42 activation kit (Cytoskeleton Inc., Denver, USA) was used according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... 300µg of total cell protein was used to pull-down GTP-bound RAS/RAF-RBD complexes according to the manufacturer’s instructions (Cytoskeleton). Activated RAS or 20µg of total cell protein were separated using NuPAGE Novex Bis-Tris gels (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were spun at maximum speed for 10mins before protein level was measured using Precision Red (Cytoskeleton, Inc., #ADV02). Samples were run using precast 4-12% NuPAGE Bis-Tris Acrylamide Gels (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Fluorescent MTs were polymerized at 2 mg/ml by incubating tubulin and rhodamine labeled tubulin (Cytoskeleton Inc.) at 10:1 ratio in BRB80 with 1 mM DTT and 1mM GTP for 5 min on ice ...
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bioRxiv - Biochemistry 2020Quote: Biotin-inactivated myosin was prepared by reacting 2 mg of rabbit skeletal muscle myosin (Cytoskeleton Cat # MYO2) with 5 μL of 250 mM EZ-Link-Maleimide-PEG11-Biotin dissolved in DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... 2 mM EGTA) supplemented with 4.51% of sucrose to adjust osmotic pressure (Cytoskeleton buffer with sucrose; CBS) and then fixed in 3% PFA in CBS for 15 min ...
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bioRxiv - Neuroscience 2022Quote: Rac1 activation was measured by using a Rac1 activation assay kit (Rac1 Pull-down Activation Assay Biochem Kit bead pulldown format, Cytoskeleton, catalog #BK035), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: RAS Activation Assay Biochem Kit™ (BK008; Cytoskeleton, Inc) was used to assess KRAS activity following manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... the microtubules were labeled by SiR700-tubulin Kit (Cytoskeleton) at a final concentration of 800 nM in cell culture medium for 2 h before imaging.
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Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavHbioRxiv - Molecular Biology 2023Quote: ... Actin polymerization biochem kit (BK003) was purchased from Cytoskeleton Inc ...
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bioRxiv - Cancer Biology 2022Quote: ... the RAS-GTP pull-down assay kit (Cytoskeleton #BK008) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... G-LISA RhoA or Rac1 activation kits from Cytoskeleton were used according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... F-actin and G-actin protein samples were collected according to the manufacturer’s instructions (#BK037, Cytoskeleton Inc., Denver, CO, USA). F-actin and G-actin were quantified by SDS-PAGE and western blot using actin antibody (#BK037 ...
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bioRxiv - Cell Biology 2020Quote: Muscle homogenates were prepared in buffer A as described above and an equal amount of protein from each sample was incubated with 20 µL of Rhotekin-RBD beads (Cytoskeleton) for 2 hours at 4°C and washed 4 times in ice-cold buffer containing 50 mM Tris (pH 7.4) ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected upon 15min of centrifugation at >15000 rpm at 4°C and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). Equal amounts of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... the samples were washed 2× with IF buffer and incubated with rhodamine phalloidin (PHDR1) (1:500) (Cytoskeleton, Inc.) for 30 minutes in the dark at 4 °C ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were then stimulated with vehicle or 100ng/ml EGF for 15 minutes or 2 hours and lysed with Cell Lysis Buffer (50mM Tris pH 7.5, 10mM MgCl2, 0.5M NaCl, 2% Igepal) (Cytoskeleton Inc.) containing protease and phoshatase inhibitors (Pierce/Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... 2% BSA) prior to immunostaining for 20 min with a 1:500 dilution of Acti-stain-555 (Cytoskeleton Inc). The ratio of cytoplasmic actin to cortical actin was measured using ImageJ (Fiji 2.1.0/1.53c ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed 2 times with 1xPBS and then incubated with rhodamine phalloidin (PHDR1; 1:500) (Cytoskeleton Inc.) in IF wash buffer for 30 min at RT in the dark ...
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bioRxiv - Microbiology 2022Quote: Proteins were collected in cell lysis buffer (50mM Tris pH 7.5, 10mM MgCl2, 0.5M NaCl, 2% Igepal) supplemented with 1X Protease inhibitor cocktail (Cytoskeleton, PIC02). Lysates were cleared by 1min centrifugation at 10,000g at 4°C and the collected supernatants were snap-frozen in liquid nitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... phenol-red free DMEM and incubated with MemGlow dyes (MemGlow 560, MG02-2 and MemGlow 590, MG03-10; Cytoskeleton) in serum-free ...
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bioRxiv - Cell Biology 2020Quote: ... the Cd42 G-LISA kit (Cytoskeleton Inc., Denver, CO, USA) was used according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... For ubiquitin pulldown assays using the SignalSeeker kit (Cytoskeleton Inc), cells were lysed in provided BlastR buffer with protease inhibitor and NEM ...
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bioRxiv - Genomics 2024Quote: ... and 500 nM SiR-tubulin Kit (Cytoskeleton, Denver, Colorado, U.S.) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... 5 μL of the filtered supernatant was pipetted into a clear flat-bottom UV plate and 250 μL of 1x Advanced Protein Assay Reagent (Cytoskeleton Inc., CO) was added and mixed ...
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bioRxiv - Cell Biology 2019Quote: ... colorimetric G-LISA activity assay kit (BK128-S, Cytoskeleton, Denver, Colorado) was used according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: Bulk Rac1 activity was measured using an assay kit (BK035, Cytoskeleton), as previously described (Hara et al. ...
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bioRxiv - Neuroscience 2022Quote: ... G-LISA RhoA activation assay biochem kit (Absorbance based) (Cytoskeleton, BK124) was used according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: The RhoA Activation Assay Biochem Kit (bead pull-down format, Cytoskeleton) was used for performing the pull-down assay on 2D monolayers that were either uninduced or induced for the (DN ...