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Citations for Cytoskeleton :
101 - 150 of 175 citations for 6 PYRROLIDIN 1 YLNICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Pharmacological treatments included 1 unit/ml Rho Activator I (CN01; Cytoskeleton, Denver, CO), 1 mM TAK165 (Mubritinib ...
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bioRxiv - Cell Biology 2021Quote: ... pH adjusted to 6.9 with KOH] supplemented with 1 mM GTP (Cytoskeleton BST06) and kept on ice ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used: rabbit anti-cofilin (Cytoskeleton ACFL02; 1:500), rabbit anti-Ser3-phosphorylated cofilin (Cell Signaling Technology #3311 ...
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bioRxiv - Biophysics 2021Quote: Cells were incubated with 1-2 μM of SiR-Actin (Cytoskeleton, Inc. Spirochrome) for 15-30 min in calcium-free buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Solutions of 434 nM His-CLASP2α or 1 μM α-actinin (Cytoskeleton, Inc.) were incubated with and without 15 μM phalloidin-stabilized F-actin for 20 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... The following stains were also used: ActiStain Phalloidin (Phdh1, Cytoskeleton Inc, 1:500), DAPI (Thermoscientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies used were mouse anti-Rac1 (1:500, Cytoskeleton Inc, #ARC03) and rabbit anti-GAPDH (1:3000 ...
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bioRxiv - Neuroscience 2023Quote: Cultured ependymocytes were treated with 1 μM Sir-Actin (Cytoskeleton, Denver, CO, USA) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in MEDIA supplemented with 1 μM SiR-Tubulin (Cytoskeleton, Inc) for 30-60 minutes prior to washing out the SiR-Tubulin with fresh DMEM before imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... The media was replaced with fresh media containing MemGlow 488 (Cytoskeleton; 1:200) and cells were incubated for 20 min at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... a highly specific fluorescent dye for F-actin (1:250, Cytoskeleton, Inc., CY-SC001), for 3 hours at room temperature ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... labeled at a 1:40 ratio with HiLyte Fluor 647 tubulin (Cytoskeleton, Denver, CO), were flowed into the chamber and immobilized by interaction with rigor kinesin-1E236A ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... Actin-F was labelled with Sir-Actin dye (1:5000, Cytoskeleton, Denver, CO, USA) for 4 h prior to infection.
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bioRxiv - Cell Biology 2023Quote: ... labeled at a 1:40 ratio with HiLyte Fluor 488 tubulin (Cytoskeleton, Denver, CO), were flowed into the chamber and immobilized by interaction with rigor kinesin-1E236A.
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bioRxiv - Biochemistry 2023Quote: ... microtubules were polymerized from 5 mg mL-1 porcine tubulin (Cytoskeleton Inc., Denver, CO) at 37 °C for 20 min in BRB80 supplemented with 1 mM GTP and then diluted 1:10 in BRB80 + 20 µM taxol ...
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bioRxiv - Neuroscience 2023Quote: ... F-actin staining was done using Acti-stain 555 phalloidin (1:2000, Cytoskeleton, PHDH1A) and counter stained with DAPI.
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bioRxiv - Cell Biology 2019Quote: ... Taxol-stabilized microtubules assembled from unlabeled and fluorescently-labeled porcine tubulin (10:1 ratio; Cytoskeleton) were introduced into the chamber ...
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bioRxiv - Biophysics 2021Quote: ... Washed cells were diluted 1:9 and stained with 50 nM SiR-actin (Spirochrome/Cytoskeleton) for 30 minutes (Lukinavicius et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Taxol-stabilized microtubules assembled from unlabeled and fluorescently-labeled porcine tubulin (10:1 ratio; Cytoskeleton) were introduced into the chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used included anti-β-actin (1:1,000, AAN01, Cytoskeleton, Inc., Denver, CO, USA), anti-AMPKα (1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with a 1:1000 dilution of SPY555-Actin Probe (CY-SC202, Cytoskeleton) in complete DMEM at 37°C for three hours prior to imaging ...
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bioRxiv - Biophysics 2023Quote: ... F-actin was labeled with rhodamine-phalloidin (PHDR1; Cytoskeleton, Inc., Denver, CO, USA; 1:167).
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bioRxiv - Bioengineering 2023Quote: ... with cell morphology and nucleus visualized using Acti-stain 555 phalloidin (1:2000, Cytoskeleton, PHDH1A) and DAPI (2ng/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Phalloidin (Alexa Fluor 488 and 670, Cytoskeleton Jomar, PHDG1-A, PHDN1-A, ICC, 1:500).
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bioRxiv - Developmental Biology 2024Quote: ... Cysts were incubated in a culture medium containing SPY650-actin (Cytoskeleton, Inc., 1:2000 dilution) for 3 h before indentation ...
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bioRxiv - Cell Biology 2020Quote: ... and then placed in 1.5 ml imaging media containing 1 μM SiR-DNA (CY-SC007, Cytoskeleton) + 10 μM Verapamil (for nucleus staining ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μm carboxylate beads (01-02-103, micromer) were coated with biotinylated laminin (LMN03, Cytoskeleton, Inc.) or FN7-10 (a four-domain segment of fibronectin responsible for cell binding and containing the RGD and PHSRN motifs102 ...
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bioRxiv - Biochemistry 2020Quote: ... Fixed tissues were washed in PBS (and stained with SiR-actin (1:1000 dilution, Cytoskeleton Inc.) for 3 hours at room temperature (RT ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained with 1 µM SiR-actin and 10 µM verapamil (Cytoskeleton, Inc., CY-SC001) for 1-3 hours before imaging ...
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bioRxiv - Microbiology 2020Quote: ... The cells were washed twice with PBS and incubated with rhodamine phalloidin (1:500) (Cytoskeleton Inc.) in IF washing buffer for 30 minutes at room temperature in the dark ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated with monoclonal mouse anti-human RhoA primary antibody (# GL01A; 1:250; Cytoskeleton Inc.), followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02 ...
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bioRxiv - Cell Biology 2019Quote: ... followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02; 1:62.5; Cytoskeleton Inc.) on a shaker at 300 rpm at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... 0.025% saponin and 1 mM ATP were added followed by 0.45 μM rhodamine actin (Cytoskeleton Inc). The culture medium was carefully removed by pipette and enough permeabilization buffer was added to cover cells ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were incubated in primary antibody diluted in TBST (1:500 anti-RHOA antibody, Cytoskeleton ARH04) or diluted in block (1:30,000 anti-a-alpha tubulin antibody ...
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bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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bioRxiv - Cell Biology 2022Quote: ... Microtubules were made by mixing 1 μl of HiLyte Fluor 488 tubulin (2 mg/ml, Cytoskeleton), 2 μl biotinylated tubulin (2 mg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were analyzed by western blot using anti-ubiquitin antibody (P4D1, Cytoskeleton AUB01-HRP, 1:1000) and visualized with Clarity Western ECL Substrate (BioRad).
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... actin filaments (Acti-stain 670 phalloidin, 1:600 dilution; Cat: SKU PHDN1, Cytoskeleton, Denver, CO USA). Imaging was performed using a spinning disk confocal microscope (Yokogawa CSU-X1 ...
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bioRxiv - Biophysics 2020Quote: ... F-actin filaments and the nuclei were labeled with 1 µM of SiR-actin (Cytoskeleton, Denver, USA) and 1 µg/ml of Hoechst 33342 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoblots were performed using the following primary antibodies: rabbit anti-KIF15 (1:1000; Cytoskeleton Inc., Cat#AKIN13), rabbit anti-KIF2A (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... 0.2 μl of 1 mg/ml HiLyte Fluor 647 or X-rhodamine-labelled tubulin (#TL670M, #TL6290M, Cytoskeleton) and 0.5 μl of 0.5 mg/ml biotin tubulin (#T333P ...
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bioRxiv - Biophysics 2019Quote: ... the primary antibodies used in the study were sheep anti-tubulin (2.5 μg mL−1, ATN02, Cytoskeleton) and mouse anti-TOM20 (2.5 μg mL−1 ...
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bioRxiv - Cell Biology 2023Quote: Stock actin (10 mg/ml) was diluted 1:10 into ATP-supplemented general actin buffer from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were stored at −20°C prior to western blot analysis for actin (Cytoskeleton 1:1000). Standards (10 ...
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bioRxiv - Developmental Biology 2023Quote: ... The explants were then incubated overnight with a 1:100 dilution of Alexa488 Acti-stain (Cytoskeleton PHDG1) and imaged using a Nikon AX confocal microscope and a10x objective.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were stained in suspension with a 1:300 dilution of Anti-Stain 488 Fluorescent Phalloidin (Cytoskeleton, #PHDG1), gently mixed ...
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bioRxiv - Neuroscience 2020Quote: ... and secondary antibody at 1 h (4 °C) facilitated by an automated western blot liquid handler (Cytoskeleton GOBlot). Antibodies utilised included anti-eIF2α (abcam ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The blocked tissue samples were then transferred to Acti-stain ™ 670 Phalloidin (1:1200, Cytoskeleton Cat#: PHDN1) reagent diluted in PBT and normal donkey serum ...
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bioRxiv - Microbiology 2023Quote: ... the samples were washed 2× with IF buffer and incubated with rhodamine phalloidin (PHDR1) (1:500) (Cytoskeleton, Inc.) for 30 minutes in the dark at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: GMPCPP-stabilized microtubules were assembled using 50 μM unlabeled porcine tubulin containing 1:12 biotin-labeled porcine tubulin (Cytoskeleton) and polymerized in the presence of 1mM GMPCPP (Jena Bioscience ...