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Citations for Cytoskeleton :
101 - 150 of 197 citations for 4 Methoxy 2 2 3 4 5 5 Hexacb Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 5 mM guanidine carbonate and 1.2 mM ATP (from Actin Polymerization Buffer, cat. BSA02, and ATP, cat. BSA04, Cytoskeleton Inc.). Samples were incubated at 37 °C for 2 h.
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bioRxiv - Cell Biology 2023Quote: ... a reaction vessel was prepared on ice containing 3 mg/ml of >97% purified porcine tubulin (Cytoskeleton, Inc. (#T240)) and the substances being tested ...
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bioRxiv - Molecular Biology 2021Quote: ... the pathway was activated with PDGF-AA at 50 ng/ml for 20 minutes at 37°C and the G-LISA assay (Cytoskeleton) was performed following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the samples were washed 2× with IF buffer and incubated with rhodamine phalloidin (PHDR1) (1:500) (Cytoskeleton, Inc.) for 30 minutes in the dark at 4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... or 60 min-old Tau:polyA or Tau:PEG:polyA condensates containing 10% Cy3-labeled polyA were imaged prior and after the addition of 5 μM tubulin (bovine cycled tubulin, PurSolutions-PUR-032005) containing 10% HiLyte647-labeled tubulin (porcine tubulin, Cytoskeleton-TL670M). For tubulin bundle formation ...
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bioRxiv - Bioengineering 2022Quote: ... The beads were cleaned 3 times in PBS and resuspended in a solution of PBS with 20 μg/ml biotinylated fibronectin (Cytoskeleton) or biotinylated BSA (Pierce ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were then stimulated with vehicle or 100ng/ml EGF for 15 minutes or 2 hours and lysed with Cell Lysis Buffer (50mM Tris pH 7.5, 10mM MgCl2, 0.5M NaCl, 2% Igepal) (Cytoskeleton Inc.) containing protease and phoshatase inhibitors (Pierce/Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... 2% BSA) prior to immunostaining for 20 min with a 1:500 dilution of Acti-stain-555 (Cytoskeleton Inc). The ratio of cytoplasmic actin to cortical actin was measured using ImageJ (Fiji 2.1.0/1.53c ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed 2 times with 1xPBS and then incubated with rhodamine phalloidin (PHDR1; 1:500) (Cytoskeleton Inc.) in IF wash buffer for 30 min at RT in the dark ...
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bioRxiv - Microbiology 2022Quote: Proteins were collected in cell lysis buffer (50mM Tris pH 7.5, 10mM MgCl2, 0.5M NaCl, 2% Igepal) supplemented with 1X Protease inhibitor cocktail (Cytoskeleton, PIC02). Lysates were cleared by 1min centrifugation at 10,000g at 4°C and the collected supernatants were snap-frozen in liquid nitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... phenol-red free DMEM and incubated with MemGlow dyes (MemGlow 560, MG02-2 and MemGlow 590, MG03-10; Cytoskeleton) in serum-free ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... the samples were preincubated for either 3 h with Rho/Rac/Cdc42 Activator I (1 µg/ml, Catalog number CN04, Cytoskeleton Inc), 30 min with MEK inhibitor PD 98059 (50 µM ...
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bioRxiv - Cancer Biology 2020Quote: ... Fixed gelatin-coated coverslips were washed six times with CMF-PBS at room temperature and coated with 50 µg/ml HiLyte488 FITC-conjugated fibronectin (Cytoskeleton, Inc., Denver, CO) for one hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Arp2/3 complex (Cytoskeleton, Inc. RP01P), and GST-WASP-VCA (Cytoskeleton ...
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bioRxiv - Biophysics 2020Quote: ... 3 μM Rhodamine tubulin (TL590M, Cytoskeleton), and 3 mM GMPCPP (NU-405S ...
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bioRxiv - Neuroscience 2021Quote: ... p-ERK1/2 (CST, 9101, 1:1000), AKT (CST, 9272, 1:1000), p-AKT (CST, 9275, 1:1000) and Cofilin (Cytoskeleton, ACFL02, 1:500) were used to quantify protein levels ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-SUMO2/3 (#12F3, Cytoskeleton Inc), Guinea Pig anti-SUMO2/3 (1:300 ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse anti-SUMO2/3 (#12F3, Cytoskeleton Inc), and Guinea Pig anti-SUMO2/3 (1:300 ...
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bioRxiv - Biophysics 2021Quote: ... the cells were rinsed thoroughly and stained with 1:200 solution of secondary antibody in PBS for 2 hours followed by staining with Acti-Stain™ 670 Fluorescent Phalloidin (Cytoskeleton.Inc, catalog no. PHDN1). Then the cover-slides were mounted on glass slides using ProLong Diamond Antifade Mountant (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... Lipids accumulated in HLOs and cytoskeleton were stained with 2 μM BODIPY® 493/503 and 1 μM SiR-Actin (Cytoskeleton Inc., CO, USA), respectively ...
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bioRxiv - Biophysics 2019Quote: ... Porcine Arp2/3 complex is purchased from Cytoskeleton and used with no further purification ...
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bioRxiv - Biophysics 2021Quote: ... and Arp2/3 complex were purchased from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2024Quote: ... anti-SUMO2/3 (Asm23, Cytoskeleton, 1:200 dilution), and anti-PML (sc966 ...
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bioRxiv - Developmental Biology 2019Quote: ... containing 50 nM silicone-rhodamine tubulin (SirTubulin) (Cytoskeleton CY-SC002) in final culture media (described above) ...
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bioRxiv - Cell Biology 2019Quote: ... or Acti-stain Phalloidin (PHDG1-A, 1:50, Cytoskeleton Inc.), followed by washes in 0.1% PBS-Triton X-100 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 60 nM Arp2/3 protein complex (#RP01P, Cytoskeleton) were also added to the actin polymerization buffer ...
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bioRxiv - Cell Biology 2021Quote: Soluble tubulin polymerization mix containing 50 μM tubulin protein (Cytoskeleton, T240), 1 μM TRITC-labeled tubulin protein (Cytoskeleton ...
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bioRxiv - Cell Biology 2020Quote: ... 3 G-LISA™ Activation Assay (purchased from Cytoskeleton Inc.), according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... The purified Arp2/3 protein complex from porcine brain (Cytoskeleton) and GST-tagged VCA domain of human WASP protein (Cytoskeleton ...
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bioRxiv - Cell Biology 2022Quote: ... 20 nM bovine brain Arp2/3 complex (Cytoskeleton; cat#RP01P), and 20 nM GST-WASP were mixed with purified Fbp17SH3 ...
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bioRxiv - Cell Biology 2022Quote: ... Cdc42 and ARP3 proteins or ARP2/3 protein complex (Cytoskeleton) were used as bait and full-length GST-ERK3 (SignalChem ...
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bioRxiv - Cell Biology 2020Quote: ... and mixed with 50 μL of 12 μM pyrene muscle actin (Cytoskeleton Inc.) in G-Buffer (5 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... the buffer also contained 50 µM non-muscle human actin protein (Cytoskeleton, Inc) and 0.2 mM ATP and 0.2 mM CaCl2.
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bioRxiv - Developmental Biology 2021Quote: ... C3 (1mg/ml, Cytoskeleton), and GO4 (100 μM ...
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bioRxiv - Biophysics 2021Quote: ... After a 9 minute incubation with 50 nM SiR-Actin (Cytoskeleton Inc., CY-SC001), the sam-ple was incubated with Prolong Live Antifade Reagent (In-vitrogen P36975 ...
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bioRxiv - Biophysics 2021Quote: ... Washed cells were diluted 1:9 and stained with 50 nM SiR-actin (Spirochrome/Cytoskeleton) for 30 minutes (Lukinavicius et al. ...
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bioRxiv - Microbiology 2022Quote: ... around 3× 107 trophozoites were incubated in cell lysis buffer (Cytoskeleton Inc, Colorado, USA) with vigorous agitation at 4°C for 30 min and homogenized by a 23-gauge needle on a 5-ml syringe ...
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bioRxiv - Plant Biology 2020Quote: ... 13 nM Arp2/3 protein complex and 15 nMWASp-VCA domain-GST purified (Cytoskeleton, USA) along with freshly purified ArF-BAR protein in concentrations of 200 ...
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bioRxiv - Cell Biology 2022Quote: ... Pyrene-labeled actin was incubated with recombinant ARP2/3 protein complex alone (Cat# RP01P, Cytoskeleton) or along with the human recombinant proteins ...
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bioRxiv - Biochemistry 2023Quote: 10 mg/mL tubulin (Cytoskeleton T240) and 1 mg/mL biotin-tubulin (Cytoskeleton T333P ...
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bioRxiv - Cancer Biology 2023Quote: ... Pig microtubules (60μg/ml, Cytoskeleton, MT002) and ATP (25μM ...
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bioRxiv - Plant Biology 2019Quote: ... The seven protein subunits of the Arp2/3 protein complex were purified from bovine brain (Cytoskeleton). The GST-VCA fusion protein associated with the glutathione-agarose 4B (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... The PDMS replicates were then coated with 50 μM fibronectin in PBS (Cytoskeleton, Inc. Green Fluorescent, HiLyte 488).
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bioRxiv - Neuroscience 2021Quote: ... 1 unit (100ng)/mL EGF (Cytoskeleton, Inc.), 5 μM pirenzepine (Tocris) ...
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bioRxiv - Cell Biology 2021Quote: ... 1.2 mL 10 mM Taxol (Cytoskeleton TXD01), 5 mL DMSO (Cytoskeleton)] was added to the stock microtubule solution at room temperature.
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bioRxiv - Cell Biology 2021Quote: ... or 1μg/ml C3 Transferase (Cytoskeleton, Inc) for at least 4 hours before fixing and staining to analyze E-cadherin localization ...
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bioRxiv - Cell Biology 2021Quote: ... and 4µL 10mg/mL TMR-tubulin (Cytoskeleton) at 37°C for 40min ...
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bioRxiv - Cell Biology 2021Quote: ... and 1.5µL 1mg/mL TMR-tubulin (Cytoskeleton) was incubated at 37°C for 1min ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... Rho activator CN03 (Cytoskeleton, Inc.; 1μg/mL), Small GTPases (Rho/Rac/Cdc42 ...
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bioRxiv - Cell Biology 2024Quote: ... Rho-Activator II (Cytoskeleton CN03, 2µg/ml), or ROCK inhibitor y-s7632 (Peprotech 1293823 ...