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Citations for Cytoskeleton :
51 - 71 of 71 citations for Recombinant Human X Linked Inhibitor of Apoptosis AVI tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... the buffer also contained 50 µM non-muscle human actin protein (Cytoskeleton, Inc) and 0.2 mM ATP and 0.2 mM CaCl2.
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bioRxiv - Biochemistry 2024Quote: ... Taxol-stabilized human unmodified microtubules assembled with 1.5% biotinylated tubulin (Cytoskeleton, Inc. #T333P) and 1% HiLyte FluorTM 488-labeled tubulin (Cytoskeleton ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell-permeable C3 transferase-based Rho inhibitor 1 was from Cytoskeleton. Saracatinib and defactinib were from Selleck Chemicals ...
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bioRxiv - Cell Biology 2022Quote: ... The following inhibitors were used: C3 exoenzyme (1 mg/ml; Cytoskeleton, Inc), NSC23766 (50 mM ...
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bioRxiv - Cell Biology 2022Quote: ... and 1x De-Ubiquitination/ SUMOylation inhibitor (N-ethylmaleimide and TPEN, NEM09BB, Cytoskeleton)) ...
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bioRxiv - Microbiology 2020Quote: ... The treatment consisted of 2 µg/ml of Rho inhibitor I (Cytoskeleton, CT04) for 2 h or 20 µM of Y27632 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were stored in G-LISA lysis buffer with manufacturer’s protease inhibitors (Cytoskeleton). Protein concentrations were determined using Precision Red Advanced Protein Assay Reagent with absorbance at 600 nm measured spectrophotometer ...
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bioRxiv - Cell Biology 2021Quote: ... 0.2 μl of 1 mg/ml HiLyte Fluor 647 or X-rhodamine-labelled tubulin (#TL670M, #TL6290M, Cytoskeleton) and 0.5 μl of 0.5 mg/ml biotin tubulin (#T333P ...
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bioRxiv - Biochemistry 2020Quote: ... For measurements including actin (human platelet actin, 99% pure, from Cytoskeleton, Inc., catalog nr: APHL99), the buffer was 5 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... Y27632 from Calbiochem (Cat Number: 688000) and C3 toxin (Rho Inhibitor I) from Cytoskeleton (Cat Number: CT04).
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bioRxiv - Cell Biology 2019Quote: ... and then incubated with monoclonal mouse anti-human RhoA primary antibody (# GL01A; 1:250; Cytoskeleton Inc.), followed by a polyclonal goat anti-mouse horseradish-conjugated secondary antibody (# GL02 ...
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bioRxiv - Cell Biology 2021Quote: ... Tubulin was labeled in 1:10 proportions with biotin or X-rhodamine according to published protocols or mixed with prelabeled tubulin purchased from Cytoskeleton, Inc.
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bioRxiv - Biophysics 2023Quote: ... we performed additional tests: incubation with 1.0 μg/ml C3-transferase (RhoA inhibitor, Cytoskeleton, cat. CT04) and with 1.0 μg/ml of RhoA activator (Cytoskeleton ...
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bioRxiv - Biophysics 2021Quote: ... or biotin labelled Sus scrofa brain tubulin - except X-rhodamine which was from Bos taurus -(catalogue numbers T240C, TL620M T333P, respectively, all >99% pure, Cytoskeleton Inc.) was reconstituted to 10 mg/mL in BRB80 (80 mM PIPES pH 6.8 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then permeabilized for 10 minutes with 0.1% Triton X-100 and stained with Rhodamine Phalloidin for 30 minutes (Cytoskeleton Inc., Denver, CO, USA). Cells were then washed in DPBS and mounted with DAPI-containing Vectashield mounting media (Vector Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were then incubated with 30 µg of Ras-GTP Binding Domain (RBD of human c-Raf kinase) fused with GST associated with beads (#RF02, Cytoskeleton) at 4°C for 1h ...
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bioRxiv - Microbiology 2022Quote: Proteins were collected in cell lysis buffer (50mM Tris pH 7.5, 10mM MgCl2, 0.5M NaCl, 2% Igepal) supplemented with 1X Protease inhibitor cocktail (Cytoskeleton, PIC02). Lysates were cleared by 1min centrifugation at 10,000g at 4°C and the collected supernatants were snap-frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... the Rho Inhibitor I ADP ribosylation of Rho Asn-41 (CT04) and Rho Activator II (CN03) were obtained from Cytoskeleton. The phosphor-ezrin inhibitor NSC668394 was from Calbiochem ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 1xPBS and lysed in appropriate volume of lysis and F-actin stabilization buffer supplemented with 1mM ATP and protease inhibitor mixture for F-actin stabilization (Cytoskeleton). Lysates were centrifuged at 100 000 x g at 37°C for 1 h to separate the G-actin fraction in the supernatants and pelleted F- actin ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were lysed in 250 µl cell lysis buffer supplemented with protease inhibitors and subjected to a pull-down assay for activated Rac1 (#BK035, Cytoskeleton). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... The exoenzyme C3 transferase (Rho inhibitor I) and the Rho activator II (CN03) were purchased from Cytoskeleton (#CT04 and #CN03, respectively). Coelenterazine 400a was purchased from NanoLight Technology (#340).