Labshake search
Citations for Cytoskeleton :
51 - 94 of 94 citations for Recombinant Human RHOA His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... Cell lysates were harvested as outlined in the G-lisa RhoA Activation Assay Kit (Cytoskeleton Inc) using the supplied lysis buffer ...
-
bioRxiv - Developmental Biology 2021Quote: ... Membranes were incubated in primary antibody diluted in TBST (1:500 anti-RHOA antibody, Cytoskeleton ARH04) or diluted in block (1:30,000 anti-a-alpha tubulin antibody ...
-
bioRxiv - Cell Biology 2020Quote: ... the membrane was probed using the iBind flex kit and a primary anti-RhoA antibody (Cytoskeleton) at 1:200 and HRP-conjugated secondary (1:4000) ...
-
bioRxiv - Biophysics 2023Quote: ... we performed additional tests: incubation with 1.0 μg/ml C3-transferase (RhoA inhibitor, Cytoskeleton, cat. CT04) and with 1.0 μg/ml of RhoA activator (Cytoskeleton ...
-
bioRxiv - Neuroscience 2023Quote: ... then rinsed and sequentially incubated with primary antibodies to RhoA followed by HRP secondary antibodies (Cytoskeleton), and the colorimetric reaction was performed for 15 min at 37 °C ...
-
bioRxiv - Cell Biology 2020Quote: In order to constitutively activate RhoA vessels at day 4 were perfused with Rho Activator II (Cytoskeleton) at a concentration of 2 µg/mL for four hours prior to use in permeability tests or fixation for immunocytochemistry ...
-
bioRxiv - Molecular Biology 2022Quote: ... The levels of RhoA and RhoB activity were measured using the Rho Activation Assay Biochem Kit (Cytoskeleton) with Rhotekin pulldown according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2022Quote: ... The membrane was then probed with a mouse monoclonal anti-RhoA antibody (supplied with the Cytoskeleton Kit) at a working dilution of 1:500 overnight at 4 °C with constant agitation ...
-
bioRxiv - Cancer Biology 2021Quote: ... GTP-pulldown and western blot procedures were performed according to RHOA/RAC1/CDC42 Activation Assay Combo kit (Cytoskeleton) manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... cells lysates were prepared according to manufacturer’s instructions (RhoA Pull-Down Activation Assay Biochem Kit; Cytoskeleton Inc., BK036). Specifically ...
-
bioRxiv - Immunology 2020Quote: ... Rac and Cdc42 was measured in lysates from negatively sorted neutrophils from all genotypes by performing respective activation G-LISA assays (RhoA, Rac1,2,3 and Cdc42 G-LISA Activation Assays; Cytoskeleton) on fibronectin-coated plates as per manufacturer’s protocol.
-
bioRxiv - Cancer Biology 2021Quote: ... GTP-bound RhoA and Rac1 were immunoprecipitated from cells according to manufacturers’ protocols (BK-036, BK-128, Cytoskeleton).
-
bioRxiv - Cell Biology 2024Quote: ... Rho-GTP was measured using the RhoA G-LISA Activation Assay Kit (Colorimetric Format; Cat# BK124) from Cytoskeleton, according to the manufacturer’s protocol.
-
bioRxiv - Microbiology 2020Quote: ... recombinant human cofilin-1 (CFL1 P23528) and arp2/3 complex (ARPC4 Q148J6) from bovine brain were all purchased from Cytoskeleton Inc ...
-
bioRxiv - Systems Biology 2022Quote: The Rho activity was measured and quantified by using the RhoA Activation Assay Biochem Kit (cat. no. BK036; Cytoskeleton) based on the Rhotekin pull-down assay as per the manufacturer’s instructions as described previously(39) ...
-
bioRxiv - Biochemistry 2022Quote: ... colorimetric Rac1 and RhoA G-LISA Activation Assay Kits (BK128-S and BK124-S Cytoskeleton Inc., Denver CO, USA) were used according to the manufacturer’s instructions.
-
bioRxiv - Biophysics 2020Quote: ... Rac1-GTP and Cdc42-GTP using the RhoA and Cdc42 pull-down activation assay biochem kit (PN:BK036-S and BK034-S, Cytoskeleton, Inc.) following the protocol provided by the manufacturer ...
-
bioRxiv - Cell Biology 2023Quote: RhoAGTP and Rac1GTP pull down as-says were performed using the RhoA and Rac1 Activation Assay Biochem Kit (Cytoskeleton BK030) according to the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2020Quote: RhoA activation was assessed with the G-LISA activation kit in accordance with the manufacturer’s instructions (Cytoskeleton Inc., catalog no. BK124-S). Active GTP-bound RhoA was detected with HRP-conjugated anti-RhoA antibodies ...
-
bioRxiv - Cancer Biology 2024Quote: ... The RhoActivity assay was performed using the RhoA pull-down activation assay biochem kit (Bead pull-down format) (Cytoskeleton, Cat No. BK036). The assay was carried out by incubating 800 µg of total cell lysate with 50 µg of Rhotekin as described in the manufacturers protocol following which western blots were performed ...
-
bioRxiv - Neuroscience 2019Quote: ... cells were rinsed with warmed PBS and cell lysates prepared in cell lysis buffer as instructed and provided by the RhoA or Rac1 pull-down instruction manual and kit (Cytoskeleton, Inc. Denver, CO). Protein quantitation was performed by BCA method and 600 μg of protein was incubated with 50 μg Rhotekin-RBD beads for RhoA pull-down or with 10 µg of PAK-PBD beads for Rac1 pull-down at 4°C for 1 h ...
-
bioRxiv - Biochemistry 2024Quote: ... and His-DBS (DH/PH domain) were purchased from Cytoskeleton, Inc ...
-
bioRxiv - Biochemistry 2023Quote: ... rhodamine- and Hi-Lyte647-labelled porcine tubulin were purchased from Cytoskeleton (T240 ...
-
bioRxiv - Cancer Biology 2023Quote: ... NCI-H295R cells were fluorescently tagged using MemGlow 560 (Cytoskeleton, Inc., Denver, CO) following manufacturer instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... the purified His-MBP-NRIPs were incubated with F-actin (AD99, Cytoskeleton) in 1 ml of IP buffer (50 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2022Quote: ... Solutions of 434 nM His-CLASP2α or 1 μM α-actinin (Cytoskeleton, Inc.) were incubated with and without 15 μM phalloidin-stabilized F-actin for 20 minutes at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: To analyze the competition between actin and His-Rap1b (Cytoskeleton Inc, cat. no. RR02-A) biding to GST SPN some modifications were made to the assay ...
-
bioRxiv - Cell Biology 2023Quote: Human platelet actin (Cytoskeleton Inc.) was polymerized in F-actin buffer (5 mM Tris-HCl ...
-
bioRxiv - Biophysics 2022Quote: ... were cultured on fiber scaffolds (for training data set generation, treatment with rhodamine tagged fibronectin (Cytoskeleton Inc, CO)) in 5% CO2 and 37°C ...
-
bioRxiv - Cancer Biology 2019Quote: Human non muscle actin (Cytoskeleton, Inc.) in general actin buffer (5 mM Tris-HCl [pH 8.0] and 0.2 mM CaCl2 ...
-
bioRxiv - Biochemistry 2020Quote: ... human platelet actin (Cytoskeleton Inc., APHL99), were mixed in equimolar ratios at 50 μM and incubated on ice for 30 minutes prior to measurement.
-
bioRxiv - Cell Biology 2020Quote: Purified human platelet actin (APHL99, Cytoskeleton) was polymerized following the manufacturer’s protocol and attached to polylysine coated coverslips by incubation with 2 µg/ml actin in actin polymerization solution ...
-
bioRxiv - Cancer Biology 2023Quote: Human non-muscle actin (Cytoskeleton, # APHL99) was polymerized or de-polymerized according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... Pyrene-labeled actin was incubated with recombinant ARP2/3 protein complex alone (Cat# RP01P, Cytoskeleton) or along with the human recombinant proteins ...
-
bioRxiv - Neuroscience 2023Quote: ∼30µg of purified recombinant TDP-43 and 10µg of rabbit skeletal muscle actin (Cytoskeleton, #BK037) were incubated with Ni-NTA beads at 4°C overnight on a rotating wheel ...
-
bioRxiv - Genetics 2022Quote: Purified (>99%) human actin monomers (Cytoskeleton, APLC99) were prepared (5 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Cell Biology 2023Quote: Stable HeLa cells that had been induced to express GFP-tagged ESCO1 transgenes for 24 hours were incubated with 250nM SIR-DNA (Cytoskeleton Inc.) and 2µg/ml doxycycline in Opti-MEM (Gibco ...
-
bioRxiv - Neuroscience 2019Quote: ... and 5 μM β-actin (human platelets; Cytoskeleton) whose pH was adjusted to 7.25 with KOH ...
-
bioRxiv - Biophysics 2022Quote: ... Lyophilized human cofilin 1 was purchased from Cytoskeleton (CF01) and reconstituted in MB buffer (20 mM MOPS pH 7.4 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Human full-length gelsolin was purchased from Cytoskeleton (US).
-
bioRxiv - Biophysics 2021Quote: ... the buffer also contained 50 µM non-muscle human actin protein (Cytoskeleton, Inc) and 0.2 mM ATP and 0.2 mM CaCl2.
-
bioRxiv - Biochemistry 2024Quote: ... Taxol-stabilized human unmodified microtubules assembled with 1.5% biotinylated tubulin (Cytoskeleton, Inc. #T333P) and 1% HiLyte FluorTM 488-labeled tubulin (Cytoskeleton ...
-
bioRxiv - Biochemistry 2020Quote: ... For measurements including actin (human platelet actin, 99% pure, from Cytoskeleton, Inc., catalog nr: APHL99), the buffer was 5 mM Tris ...
-
bioRxiv - Cell Biology 2021Quote: ... Lysates were then incubated with 30 µg of Ras-GTP Binding Domain (RBD of human c-Raf kinase) fused with GST associated with beads (#RF02, Cytoskeleton) at 4°C for 1h ...