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Citations for Cytoskeleton :
51 - 97 of 97 citations for 7 Chloro 9 methyl 3 4 dihydro 2H benzo b oxepin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Non-muscle actin (5 µM, APHL99, Cytoskeleton Inc.) was included in the pipette to prevent wash-out of plasticity63 ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μM β-actin (human platelets; Cytoskeleton) whose pH was adjusted to 7.25 with KOH ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 μg/mL RhoA Activator II (Cytoskeleton, Inc.) or 2 μg/mL Rho A Inhibitor I (Cytoskeleton ...
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bioRxiv - Developmental Biology 2020Quote: ... were microinjected into wild type embryos at one-cell stage together with 1.3 ng HiLyte Fluor™ 488-Tubulin (TL488M, Cytoskeleton, Inc.) or 1.3 ng TAMRA Rhodamine Tubulin (TL590M ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μl of soluble TRITC-labelled tubulin (Cytoskeleton, TL590M) in phase separation buffer containing 500 ng ml−1 nocodazole was added for a final tubulin concentration of 5 μM ...
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bioRxiv - Biophysics 2023Quote: 5 μM porcine brain tubulin (Cytoskeleton, Cat No. T240) and 5 μM MAP7 MTBD were mixed with 2 mM GTP (final concentration ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were fixed in PFA (4% PFA in Cytoskeleton Buffer (CB) – in mM ...
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bioRxiv - Biophysics 2021Quote: ... 5 μM F-actin (mixture 20% Biotinylated actin (Cytoskeleton Inc.)) was added to the mixture and incubated at RT for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... around 3× 107 trophozoites were incubated in cell lysis buffer (Cytoskeleton Inc, Colorado, USA) with vigorous agitation at 4°C for 30 min and homogenized by a 23-gauge needle on a 5-ml syringe ...
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bioRxiv - Cell Biology 2021Quote: ... 22 μl of 5 mg ml−1 tubulin protein (Cytoskeleton, T240) was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton ...
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bioRxiv - Microbiology 2023Quote: ... and ∼5% Alexa488-labeled or Alexa647-labeled fluorescent tubulin (Cytoskeleton inc.) were prepared as described64 and stabilized with final 20µM taxol (Cytoskeleton inc.) ...
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bioRxiv - Plant Biology 2020Quote: ... 13 nM Arp2/3 protein complex and 15 nMWASp-VCA domain-GST purified (Cytoskeleton, USA) along with freshly purified ArF-BAR protein in concentrations of 200 ...
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bioRxiv - Cell Biology 2022Quote: ... Pyrene-labeled actin was incubated with recombinant ARP2/3 protein complex alone (Cat# RP01P, Cytoskeleton) or along with the human recombinant proteins ...
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bioRxiv - Microbiology 2024Quote: Microtubules were made by mixing 4 μg of HiLyte Fluor 488 tubulin (Cytoskeleton), 4 μg of biotinylated tubulin (Cytoskeleton ...
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bioRxiv - Biophysics 2020Quote: ... 5 μL of 10x polymerization buffer solution (BSA02; Cytoskeleton, Inc., Denver, CO) mixed with 45 μL of the previously produced solution were left for 1h at room temperature to polymerize ...
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bioRxiv - Cell Biology 2022Quote: ... 0.4 µl of 5 mg/ml biotin labeled tubulin (Cytoskeleton, #T333P-A), 20% glycerol ...
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bioRxiv - Biophysics 2019Quote: MTs were prepared by polymerizing 5 mg/ml tubulin (Cytoskeleton, Denver, CO) in BRB80 buffer (80 mM PIPES ...
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bioRxiv - Cell Biology 2022Quote: ... in a ratio of 1:5 and then stabilizing with paclitaxel (Cytoskeleton). MTs and test proteins were incubated in binding buffer A (80 mM Pipes ...
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bioRxiv - Biochemistry 2023Quote: ... cells were pre-extracted for 5 minutes with cold CSK buffer (‘Cytoskeleton buffer’ ...
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bioRxiv - Plant Biology 2019Quote: ... The seven protein subunits of the Arp2/3 protein complex were purified from bovine brain (Cytoskeleton). The GST-VCA fusion protein associated with the glutathione-agarose 4B (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... cell lysate (125 μg) was added to 5 μg GGA3-PBD beads (Cytoskeleton, USA) to pull down ARFs-GTP and incubated at 4°C on a rotator for 1h and then the beads were extensively washed to remove unbound protein ...
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bioRxiv - Biochemistry 2023Quote: ... microtubules were polymerized from 5 mg mL-1 porcine tubulin (Cytoskeleton Inc., Denver, CO) at 37 °C for 20 min in BRB80 supplemented with 1 mM GTP and then diluted 1:10 in BRB80 + 20 µM taxol ...
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bioRxiv - Biophysics 2021Quote: ... Increasing concentrations of tropomyosins (0.2-3.5 μM) were mixed and incubated with 3 μM rabbit skeletal-muscle F-actin (Cytoskeleton, Inc.) in T buffer at a final reaction volume of 50 μL for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... a reaction vessel was prepared on ice containing 3 mg/ml of >97% purified porcine tubulin (Cytoskeleton, Inc. (#T240)) and the substances being tested ...
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bioRxiv - Cell Biology 2023Quote: ... rhodamine-labeled MTs were polymerized using a mixture containing 5% rhodamine-labeled tubulin (Cytoskeleton, TL590M), 5% biotin-labeled tubulin (Cytoskeleton ...
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bioRxiv - Cell Biology 2020Quote: In order to constitutively activate RhoA vessels at day 4 were perfused with Rho Activator II (Cytoskeleton) at a concentration of 2 µg/mL for four hours prior to use in permeability tests or fixation for immunocytochemistry ...
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bioRxiv - Microbiology 2020Quote: ... Samples were fixed in 4% (v/v) Paraformaldehyde Aqueous Solution (15710-s, EM Grade) in PEM (Cytoskeleton-preserving buffer PIPES-EGTA-Magnesium ...
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bioRxiv - Microbiology 2020Quote: ... recombinant human cofilin-1 (CFL1 P23528) and arp2/3 complex (ARPC4 Q148J6) from bovine brain were all purchased from Cytoskeleton Inc ...
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bioRxiv - Bioengineering 2022Quote: ... The beads were cleaned 3 times in PBS and resuspended in a solution of PBS with 20 μg/ml biotinylated fibronectin (Cytoskeleton) or biotinylated BSA (Pierce ...
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bioRxiv - Molecular Biology 2022Quote: ... or 60min-old Tau:RNA or Tau:RNA:PEG coacervates were mixed with 5 μM porcine tubulin-Alexa594 (Cytoskeleton), and samples were pipetted onto the glass surface of an imaging dish (MaTek ...
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bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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bioRxiv - Cell Biology 2023Quote: ... which was 5% fluorescently labelled with HiLyte 488 or HiLyte 647 conjugated porcine brain tubulin (Cytoskeleton), was assembled at 40 μM at 1 mM GTP for 30 mins at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... and secondary antibody at 1 h (4 °C) facilitated by an automated western blot liquid handler (Cytoskeleton GOBlot). Antibodies utilised included anti-eIF2α (abcam ...
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bioRxiv - Bioengineering 2019Quote: HUVECs were seeded on the PEG islands and control SiO2 substrates for 3 h and then flooded with cell culture medium containing 1:1000 dilution of SiR-DNA (Cytoskeleton Inc). An additional hour was required for cells to uptake SiR-DNA to be trackable through SiR-DNA probe using Leica DMI6000 microscope ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... the samples were preincubated for either 3 h with Rho/Rac/Cdc42 Activator I (1 µg/ml, Catalog number CN04, Cytoskeleton Inc), 30 min with MEK inhibitor PD 98059 (50 µM ...
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bioRxiv - Cell Biology 2020Quote: A solution of 5 μg/mL Fibronectin labeled with HiLyte fluor 488 (FNR02-A, Cytoskeleton, Inc. USA) in PBS was incubated on glass bottom petri dish for 30 min at 4°C and rinsed with cold PBS thrice ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates were centrifugated for 15 min at > 15000 rpm at 4°C and supernatant was collected and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... actomyosin contractility of MECs stably expressing an inducible ROCK construct was induced overnight using 4-hydroxytamoxifen and the actin cortex of these cells was labelled with SiR-actin dye (Cytoskeleton) according to manufacturer’s instructions prior to laser ablation.
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bioRxiv - Developmental Biology 2023Quote: ... Supernatant was collected upon 15min of centrifugation at >15000 rpm at 4°C and quantified by Precision Red Advanced Protein Assay (Cytoskeleton). Equal amounts of protein were boiled in NuPAGE LDS sample buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: Mouse antibodies against Myc-tag (M185-3L/M047-3, MBL, Nagoya, Japan), rabbit antibody against GFP-tag (D110008, BBI, Shanghai, China) and sheep antibody against Tubulin (ATN02, Cytoskeleton, Denver, United States) were subjected to Western blot according to the manufacturer’s protocol ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: Dynamic MT seed assays were performed by polymerizing GMPCPP stabilized Cy5-MT seeds from 4 µM HiLyte 647 tubulin (Cytoskeleton, #TL670M) in BD buffer + 0.5 mM GMPCPP at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Imaging solution consisted of indicated concentration of porcine brain tubulin (5% or 15% rhodamine-labeled, Cytoskeleton Inc., Denver CO) and Alexa488-labeled tau in BRB80 supplemented with 1 mM GTP ...
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bioRxiv - Cell Biology 2022Quote: ... before incubating for 5 minutes at room temperature in 40 ng/µl rhodamine-labeled actin (Cat# AR05, Cytoskeleton, Inc.) freshly prepared in cytoskeletal buffer ...
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bioRxiv - Biochemistry 2021Quote: Porcine Tubulin at 1:5 ratio (rhodamine-labeled: unlabeled) (cat. no. T240 and TL590M-A respectively, Cytoskeleton, Inc., Denver, USA) was reconstituted to 4.0 mg/mL in cold polymerization buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM guanidine carbonate and 1.2 mM ATP (from Actin Polymerization Buffer, cat. BSA02, and ATP, cat. BSA04, Cytoskeleton Inc.). Samples were incubated at 37 °C for 2 h.
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bioRxiv - Biophysics 2019Quote: ... This is done by mixing 5 μL sonicated GMPCPP microtubule (1 mg/mL short microtubules prepared from 97% pure tubulin (HTS03-A, Cytoskeleton, Inc), stored at −80 °C and thawed right before use ...
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bioRxiv - Molecular Biology 2022Quote: ... or 60 min-old Tau:polyA or Tau:PEG:polyA condensates containing 10% Cy3-labeled polyA were imaged prior and after the addition of 5 μM tubulin (bovine cycled tubulin, PurSolutions-PUR-032005) containing 10% HiLyte647-labeled tubulin (porcine tubulin, Cytoskeleton-TL670M). For tubulin bundle formation ...