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Citations for Cytoskeleton :
51 - 100 of 283 citations for 6H 1 3 5 Trioxepino 6 7 f benzimidazole 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Alexa Fluor 568 phalloidin was used to stain filamentous actin (F-actin) (3.5:500, Cytoskeleton). For detection of mucus secreting goblet cells ...
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bioRxiv - Cell Biology 2019Quote: ... Rhodamine Phalloidin used for F-actin florescence staining was purchased from Cytoskeleton (Denver, CO, USA). For immunofluorescence staining ...
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bioRxiv - Neuroscience 2023Quote: ... we used the G-actin/F-actin in vivo assay kit (Cytoskeleton Inc., Denver, USA) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... microtubules were polymerized from 5 mg mL-1 porcine tubulin (Cytoskeleton Inc., Denver, CO) at 37 °C for 20 min in BRB80 supplemented with 1 mM GTP and then diluted 1:10 in BRB80 + 20 µM taxol ...
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bioRxiv - Molecular Biology 2019Quote: Co-pelleting assay was performed according to the F-actin binding co-sedimentation assay from Cytoskeleton Inc ...
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bioRxiv - Immunology 2020Quote: ... Permeabilization and cytoskeleton staining was then performed using an F-actin Visualization Biochem Kit (Cytoskeleton Inc.). To stain for GBM-associated antigens ...
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bioRxiv - Cell Biology 2023Quote: ... All samples were processed using the “G-actin / F-actin In Vivo Assay Kit” (Cytoskeleton Inc., #BK037) which involves lysing cells in a filament-supporting buffer ...
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bioRxiv - Cell Biology 2021Quote: ... was mixed with 2 μl of 5 mg ml−1 TRITC-labeled tubulin protein (Cytoskeleton, TL590M) and 1 μl of 5 mg ml−1 Biotin-XX-labeled tubulin (Pursolutions ...
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bioRxiv - Cell Biology 2020Quote: ... Arp2/3 complex (Cytoskeleton, Inc. RP01P), and GST-WASP-VCA (Cytoskeleton ...
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bioRxiv - Cell Biology 2019Quote: ... 50 nM Arp2/3 (Cytoskeleton Inc.), 1 μM VCA (Cytoskeleton Inc. ...
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bioRxiv - Biophysics 2020Quote: ... 3 μM Rhodamine tubulin (TL590M, Cytoskeleton), and 3 mM GMPCPP (NU-405S ...
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bioRxiv - Cell Biology 2023Quote: ... 50 nM Arp2/3 (Cytoskeleton; RP01P), 1 μM VCA domain of WASP (Cytoskeleton ...
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bioRxiv - Cancer Biology 2021Quote: RHOA activity was measured on LN-F lysates using the RHOA G-LISA Activation Assay kit (#BK124, Cytoskeleton). Data are the mean ± SEM of arbitrary units of RHOA activity.
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bioRxiv - Developmental Biology 2019Quote: Actin pelleting experiments were performed using the G-Actin/F-actin In Vivo Assay Biochem Kit (Cytoskeleton, Inc.) with modifications ...
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bioRxiv - Cell Biology 2021Quote: ... and 6% fluorescent (X-Rhodamine or HiLyte647) tubulin (Cytoskeleton Inc) in the presence of the nonhydrolyzable GTP analogue GMPCPP (Jena Bioscience ...
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bioRxiv - Microbiology 2020Quote: ... recombinant human cofilin-1 (CFL1 P23528) and arp2/3 complex (ARPC4 Q148J6) from bovine brain were all purchased from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mL DMSO (Cytoskeleton)] was added to the stock microtubule solution at room temperature.
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bioRxiv - Plant Biology 2021Quote: F-actin disruption assays were carried out using the Actin Binding Protein Spin-Down Assay Biochem kit (Cytoskeleton, USA) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Actin protein levels were calculated using a G-actin/F-actin in vivo Assay Biochem Kit as per manufacturer’s instructions (Cytoskeleton). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... F-actin and G-actin were quantified by SDS-PAGE and western blot using actin antibody (#BK037, Cytoskeleton Inc.).
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-SUMO2/3 (#12F3, Cytoskeleton Inc), Guinea Pig anti-SUMO2/3 (1:300 ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse anti-SUMO2/3 (#12F3, Cytoskeleton Inc), and Guinea Pig anti-SUMO2/3 (1:300 ...
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bioRxiv - Bioengineering 2019Quote: HUVECs were seeded on the PEG islands and control SiO2 substrates for 3 h and then flooded with cell culture medium containing 1:1000 dilution of SiR-DNA (Cytoskeleton Inc). An additional hour was required for cells to uptake SiR-DNA to be trackable through SiR-DNA probe using Leica DMI6000 microscope ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... the samples were preincubated for either 3 h with Rho/Rac/Cdc42 Activator I (1 µg/ml, Catalog number CN04, Cytoskeleton Inc), 30 min with MEK inhibitor PD 98059 (50 µM ...
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bioRxiv - Cell Biology 2021Quote: ... Microtubule growth was initiated by flowing in 10.7 μM tubulin containing 7% Hilyte647–labeled tubulin (Cytoskeleton Inc.) together with 36 nM motor proteins in the reaction buffer (BRB80 with 1 mM GTP ...
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bioRxiv - Neuroscience 2022Quote: ... Day 8 iTF-Microglia were stained for F-actin using 25 nM SiR-actin (Cytoskeleton, Inc; Cat. No. CY-SC001) probe ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: Globular and filamentous actin ratios were determined by western blot as described by commercially available G-actin/ F-actin In Vivo Assay Kit (Cytoskeleton). Globular and filamentous immunocytochemistry was performed as previously described [53] ...
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bioRxiv - Microbiology 2022Quote: G- and F-actin were fractionated and enriched using a commercial in vivo assay biochem kit (Cytoskeleton Inc, Colorado, USA), according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Cancer Biology 2021Quote: ... F-actin and G-actin protein samples were collected according to the manufacturer’s instructions (#BK037, Cytoskeleton Inc., Denver, CO, USA). F-actin and G-actin were quantified by SDS-PAGE and western blot using actin antibody (#BK037 ...
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bioRxiv - Developmental Biology 2021Quote: ... cleaved proteins (quantified via a Bradford Assay) were incubated at room temperature for one hour with 0 or 5μM polymerized chicken F-actin (Cytoskeleton, Inc.). BSA was used a negative control ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volumes of G-actin and F-actin lysates were analysed by SDS-PAGE and immunoblotting with anti-actin rabbit polyclonal antibody (Cat# AANO1, Cytoskeleton). Densitometric analyses of the G-actin and F-actin levels were performed using ImageJ software ...
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bioRxiv - Biophysics 2019Quote: ... Porcine Arp2/3 complex is purchased from Cytoskeleton and used with no further purification ...
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bioRxiv - Biophysics 2021Quote: ... and Arp2/3 complex were purchased from Cytoskeleton Inc ...
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bioRxiv - Cell Biology 2019Quote: ... Microtubule growth was then initiated by flowing in 20 μM tubulin containing 7% Hilyte647-labeled tubulin (Cytoskeleton Inc.) together with cell extracts in the reaction buffer [2.5 mM GTP ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was carefully removed and the pellet was resuspended in a volume of F-actin depolymerization buffer (FAD-02, Cytoskeleton Inc.) matching the volume of the supernatant ...
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bioRxiv - Microbiology 2022Quote: ... 110 μl of LAS2 buffer was prepared according to the protocol of a G-actin/F-actin In Vivo Assay Biochem Kit (Cytoskeleton, Inc.) and mixed with the pellet ...
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bioRxiv - Neuroscience 2023Quote: F- and G-actin fractions from lysates were prepared using a G-Actin/F-Actin in Vivo Assay Biochem kit (Cytoskeleton, #BK037) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Quantification of structural change (i.e., neurite shape and growth) was performed using f-actin labeling with rhodamine phalloidin (Cytoskeleton PHDG1-A). All morphometric measures were conducted and quantified using ImageJ (NIH ...
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bioRxiv - Cell Biology 2023Quote: Western blots to determine the ratio of G-actin to F-actin were performed on retinal lysates that were processed using a G-actin/F-actin In Vivo Assay Kit (Cytoskeleton, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Porcine Tubulin at 1:5 ratio (rhodamine-labeled: unlabeled) (cat. no. T240 and TL590M-A respectively, Cytoskeleton, Inc., Denver, USA) was reconstituted to 4.0 mg/mL in cold polymerization buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... and 60 nM Arp2/3 protein complex (#RP01P, Cytoskeleton) were also added to the actin polymerization buffer ...
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bioRxiv - Biophysics 2022Quote: ... and 5 μM tubulin-AlexaFluor647 (Cytoskeleton) were combined with 1mM DTT and 0.6mM GMP-CPP in M2B buffer and incubated spun at 300,000 g to remove aggregates ...
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bioRxiv - Cell Biology 2023Quote: ... 5% biotin-labeled tubulin (Cytoskeleton, T333P), and 90% unlabeled tubulin (Cytoskeleton ...
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bioRxiv - Biophysics 2019Quote: ... This is done by mixing 5 μL sonicated GMPCPP microtubule (1 mg/mL short microtubules prepared from 97% pure tubulin (HTS03-A, Cytoskeleton, Inc), stored at −80 °C and thawed right before use ...
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bioRxiv - Cell Biology 2021Quote: ... followed by polymerization of GMPCPP-caps for 1.5 min [7 uM bovine brain tubulin (gift of R. Ohi, University of Michigan) with 11% HiLyte647-tubuiln (Cytoskeleton. Inc.) in BRB80 with 2.6 mM GMPCPP ...
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bioRxiv - Biophysics 2023Quote: ... cell clusters on Day 7 were incubated overnight with 100 nM Acti-stainTM 488 Phalloidin (Cat. #PHDG1, Cytoskeleton Inc., USA) and washed thrice with PBS ...
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bioRxiv - Bioengineering 2020Quote: ... or 3 μg/mL C3 transferase (Cytoskeleton, Inc, Denver, CO).
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bioRxiv - Cell Biology 2020Quote: ... 3 G-LISA™ Activation Assay (purchased from Cytoskeleton Inc.), according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... The purified Arp2/3 protein complex from porcine brain (Cytoskeleton) and GST-tagged VCA domain of human WASP protein (Cytoskeleton ...
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bioRxiv - Cell Biology 2022Quote: ... 20 nM bovine brain Arp2/3 complex (Cytoskeleton; cat#RP01P), and 20 nM GST-WASP were mixed with purified Fbp17SH3 ...