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Citations for Cytoskeleton :
701 - 750 of 1141 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 10 nM of Rho Activator II CN03 (Cytoskeleton, Inc), or 2 μM of CytoD (22144-77-0 ...
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bioRxiv - Cancer Biology 2022Quote: ... the RAS-GTP pull-down assay kit (Cytoskeleton #BK008) was used ...
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bioRxiv - Cancer Biology 2022Quote: ... the colorimetric-based RAS G-LISA activation assay (Cytoskeleton #BK131) was used ...
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bioRxiv - Cell Biology 2022Quote: ... tEVs were labeled using 200nM of MemGlow-Cy5 (Cytoskeleton Inc.) lipidic dye between the 1st and the 2nd 100 000g ultracentrifugation as described previously ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with MemGlow-488 (Cytoskeleton Inc.) at 200nM to label the plasma membrane.
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bioRxiv - Cell Biology 2022Quote: ... Actin-stain™ 488 Phalloidin (Cytoskeleton, PHDG1) was used at the ratio of 1:2000.
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bioRxiv - Biophysics 2022Quote: ... The PDMS replicates were then coated with 50 μM fibronectin in PBS (Cytoskeleton, Inc. Green Fluorescent, HiLyte 488).
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bioRxiv - Cell Biology 2022Quote: ... before incubating for 5 minutes at room temperature in 40 ng/µl rhodamine-labeled actin (Cat# AR05, Cytoskeleton, Inc.) freshly prepared in cytoskeletal buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein was quantified using the Precision Red (#ADV02; Cytoskeleton) advanced protein assay and 10-20μg was used ...
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bioRxiv - Cancer Biology 2022Quote: ... a Ras GTP-ase immunosorbent assay (G-LISA) Activation Assay Kit (#BK131, Cytoskeleton) was used ...
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bioRxiv - Cell Biology 2022Quote: Rho activator II (Cytoskeleton, CN03) was used at the concentration of 1 μg/mL for 3-4 h before experiments ...
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bioRxiv - Cell Biology 2022Quote: ... Actin in SIM images was stained by Actin-stain™ 488 phalloidin (Cytoskeleton, PHDG1) at 100 nM at room temperature for 30 min.
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with a 1:1000 dilution of SPY555-Actin Probe (CY-SC202, Cytoskeleton) in complete DMEM at 37°C for three hours prior to imaging ...
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bioRxiv - Biophysics 2021Quote: ... HiLyte Fluor-488-labeled tubulin (Cytoskeleton TL488M-A), rabbit skeletal actin (Cytoskeleton AKL99) ...
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bioRxiv - Biophysics 2021Quote: ... rabbit skeletal actin (Cytoskeleton AKL99), Alexa-568-labeled actin (ThermoFisher A12374) ...
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bioRxiv - Biophysics 2021Quote: Lyophilized rabbit skeletal muscle G-actin (Cytoskeleton, Inc. AKL99) was resuspended to 5 mg/mL (119 μM ...
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bioRxiv - Biophysics 2021Quote: Actin was either purchased from Cytoskeleton Inc ...
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bioRxiv - Biophysics 2021Quote: The following cytoskeleton proteins are purchased: porcine brain tubulin (Cytoskeleton T240), HiLyte Fluor-488-labeled tubulin (Cytoskeleton TL488M-A) ...
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bioRxiv - Biophysics 2021Quote: ... purified tubulin from bovine brain was utilized in the present study (catalog No. T238, Cytoskeleton, Denver, CO). Tubulin was polymerized with a “hybrid” protocol in the absence of taxol ...
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bioRxiv - Biophysics 2021Quote: ... Unlabelled porcine tubulin was obtained commercially (Cytoskeleton Inc., USA). All fine chemicals were sourced from Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... or porcine brain tubulin (Cytoskeleton Inc., CO, USA) were incubated with 1 mM GTP ...
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bioRxiv - Biophysics 2021Quote: ... Increasing concentrations of tropomyosins (0.2-3.5 μM) were mixed and incubated with 3 μM rabbit skeletal-muscle F-actin (Cytoskeleton, Inc.) in T buffer at a final reaction volume of 50 μL for 1 h at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... and stained with rhodamine phalloidin (cat # PHDR1, Cytoskeleton) for 45 min followed by three additional PBS washes ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies used were as follows: mouse anti-RAC1 (Cytoskeleton #ARC03, 1:250); mouse anti-RHOA (Cytoskeleton #ARH05 ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-RHOA (Cytoskeleton #ARH05, 1:500); rabbit anti-ROCK2 (Cell Signaling #9029 ...
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bioRxiv - Cancer Biology 2021Quote: ... GTP-pulldown and western blot procedures were performed according to RHOA/RAC1/CDC42 Activation Assay Combo kit (Cytoskeleton) manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: The microtubule polymerisation assay was performed using porcine neuronal tubulin (Cytoskeleton, Inc, BK006P) as an adaptation of the original method of Shelanski et al ...
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bioRxiv - Cancer Biology 2021Quote: ... the Rac1- and cdc42 Small GTPase Activation Assay kits (G-LISA) were used (027BK127-S, 027BK128-S, Cytoskeleton, Inc) following the company’s guidelines.
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bioRxiv - Immunology 2021Quote: ... cell lysate (125 μg) was added to 5 μg GGA3-PBD beads (Cytoskeleton, USA) to pull down ARFs-GTP and incubated at 4°C on a rotator for 1h and then the beads were extensively washed to remove unbound protein ...
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bioRxiv - Immunology 2021Quote: ... anti-ARF6 (ARF-06; Cytoskeleton, 1:500), anti-p44/42 MAPK (CST #4695 ...
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bioRxiv - Immunology 2021Quote: Activation of ARF6 (ARF6-GTP) was quantitated using the Arf6 Pull-Down Activation Assay Biochem Kit from Cytoskeleton (BK033-S, USA) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... G-actin and biotinylated actin were bought from Cytoskeleton, Inc ...
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bioRxiv - Biophysics 2021Quote: ... pH 7.5 (actin polymerization buffer, Cytoskeleton, Inc. BSA02) for an hour ...
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bioRxiv - Cell Biology 2021Quote: ... Pharmacological treatments included 1 unit/ml Rho Activator I (CN01; Cytoskeleton, Denver, CO), 1 mM TAK165 (Mubritinib ...
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bioRxiv - Developmental Biology 2021Quote: ... a highly specific fluorescent dye for F-actin (1:250, Cytoskeleton, Inc., CY-SC001), for 3 hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... then stained with Rhodamine-Phalloidin (Cytoskeleton, Inc., Cat. # PHDR1) according to manufacturer protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Acti-stain 555 phalloidin (1:140, Cytoskeleton, PHDH1-A), Actin-stain 670 phalloidin (1:140 ...
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bioRxiv - Developmental Biology 2021Quote: ... C3 (1mg/ml, Cytoskeleton), and GO4 (100 μM ...
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bioRxiv - Neuroscience 2021Quote: ... p-ERK1/2 (CST, 9101, 1:1000), AKT (CST, 9272, 1:1000), p-AKT (CST, 9275, 1:1000) and Cofilin (Cytoskeleton, ACFL02, 1:500) were used to quantify protein levels ...
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bioRxiv - Neuroscience 2021Quote: Effects of tau and nocodazole on MT polymerization were tested using a Tubulin Polymerization Assay (Cytoskeleton Inc., Denver, CO). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... 1 unit (100ng)/mL EGF (Cytoskeleton, Inc.), 5 μM pirenzepine (Tocris) ...
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bioRxiv - Neuroscience 2021Quote: ... hippocampal slices previously stabilized for 1 h with oxygenated ACSF were lysed and homogenized in the presence of conditions that stabilize F-actin and G-actin using an F/G actin commercial assay (F/G actin in vivo assay BK037, Cytoskeleton Inc., USA). Then ...
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bioRxiv - Neuroscience 2021Quote: ... and RhoA was measured using a Rho GTPase Activation Assay Combo Biochem Kit (BK030, Cytoskeleton, USA), according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2021Quote: ... F-actin pellet was resuspended in a depolymerizing buffer (BK037, Cytoskeleton Inc., USA) and then F- and G-actin fractions were diluted in loading buffer (50 mM Tris–HCl ...
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bioRxiv - Neuroscience 2021Quote: ... washed and the immunoprecipitated resolved on 12% SDS-PAGE and detected via Western blot analysis using Cdc42 (mouse anti-Cdc42, ACD03 Cytoskeleton; 1:250), Rac1 (mouse anti-Rac1 ...
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bioRxiv - Neuroscience 2021Quote: ... and RhoA (mouse anti-RhoA, ARH05 Cytoskeleton; 1:500), and visualized by ECL (Pierce ...
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bioRxiv - Neuroscience 2021Quote: ... Rac1 (mouse anti-Rac1, ARC03 Cytoskeleton; 1:500) and RhoA (mouse anti-RhoA ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were resolved on 12% SDS-PAGE and detected via Western blot analysis using actin (rabbit anti-actin, BK037 Cytoskeleton Inc.; 1:500) and visualized by ECL (Pierce ...
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bioRxiv - Molecular Biology 2021Quote: Rho-GTPase pull-down assays were performed following the manufacturer’s protocol (Cytoskeleton, Inc.). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... the pathway was first inhibited with fasudil 50µM (Bio-Techne, Minnesota, USA) or C3-exoenzyme 2µg/ml (Cytoskeleton, Denver, USA) for 15 hours and then activated with PDGF-AA at 50ng/ml for 20 minutes and proceed to the G-LISA or IF assay of myosin light chain phosphorylation (p-MLC) ...