Labshake search
Citations for Corning :
201 - 250 of 261 citations for Recombinant Mouse CTSB Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The number of cells to be injected per mouse was resuspended in PBS and mixed in a 1:1 ratio with Growth Factor Reduced Matrigel (Corning) to a final volume of 50μL per injection ...
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bioRxiv - Molecular Biology 2021Quote: ... 5,000 cells after three hours phagocytosis assay in the presence of 200 nM hybrid protein were dispensed in a 96-well plate (Corning #3596) at a total volume of 100 µL in serum-free and phenol red-free DMEM ...
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bioRxiv - Biophysics 2022Quote: ... Samples were prepared at room temperature using 0.3 μM labeled protein within the bulk of unlabeled materials and loaded into 96-well plates (Corning nonbinding surface). Sample buffer consisted of 50 mM Tris-HCl buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Mixtures (200 μL) containing YjoB and target proteins in buffer A were placed onto a 96-well microtiter plate (Corning Incorporated). Absorbance at 340 nm was recorded for 150 min with 3 min intervals using a SpectraMax iD5 microplate reader (Molecular Devices ...
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bioRxiv - Physiology 2022Quote: ... samples containing 30 μg (liver) or 15 μg (gonad) of protein were placed in a solid black 96-well plate (Corning 3912), then the reaction was initiated out of direct light by the addition of buffer containing lucigenin and NADPH ...
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bioRxiv - Microbiology 2020Quote: ... Purified NS1 protein from D2Y98P or 16681 (10 ng/well) diluted in PBS was coated onto 96-well EIA plates (Corning costar) overnight at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... 5 μg/ml RBD-SpyTag protein produced in our lab was coated onto 96-well high binding Costar® Assay plates (CORNING) at 4 °C overnight ...
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bioRxiv - Immunology 2020Quote: ELISAs to evaluate antibodies binding to SARS-CoV-2 RBD and trimeric spike proteins were performed by coating of high binding 96 half well plates (Corning #3690) with 50 μL per well of a 1μg/mL protein solution in PBS overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: Peroxidase activity was measured by diluting 20 uL of sample protein extract with 20 uL of 10 mM PBS (pH 6.0) in a 96-well plate (Costar, Corning, Kennebunk, ME). Then ...
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bioRxiv - Immunology 2023Quote: ... was measured by diluting 20 uL of coral protein extract in 20 uL of 50 mM PBS (pH 7.0) in a 96 well plate (Costar, Corning, Kennebunk, ME). In order to convert prophenoloxidase to phenoloxidase ...
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bioRxiv - Bioengineering 2024Quote: ... except the custom hydrogel formulations were replaced with growth factor deficient (GFD) Matrigel basement membrane matrix (9.2 mg/mL protein concentration, Corning, MA, USA) in both layers ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted protein sample was concentrated to a volume of 250 µl using CORNING SPIN-X concentrators (50 kDa cutoff) (Corning, 431490), followed by centrifugation 21,130 g for 15 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells expressing fluorescent proteins (mEGFP and mCherry) were plated in 384-well flat clear bottom white polystyrene TC-treated microplates (Corning, Corning, USA) and allowed to attach to plates overnight ...
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bioRxiv - Bioengineering 2024Quote: ... multilayer Matrigel constructs were made using the same methods described above except replacing the custom hydrogel formulations with Growth Factor Reduced Basement Membrane Matrigel in both layers (9.2 mg/mL protein concentration, Corning, MA, USA).
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bioRxiv - Microbiology 2024Quote: ... Purified NS1 protein from D2Y98P (10 ng/well) diluted in PBS was coated onto 96-well enzyme immunoassay plates (Corning Costar) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... MC38 cells (5 × 105 cells/mouse) were prepared in HBSS−/− containing 1:1 v:v of growth-factor reduced matrigel (Corning Life Sciences) and injected subcutaneously in the flank of female C57BL/6j mice ...
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bioRxiv - Immunology 2020Quote: Human iPSCs were initially transferred from mouse embryonic feeder layers to feeder-free conditions and thereafter maintained on a selected batch of Matrigel® (Corning) in mTeSR™1 (STEMCELL Technologies ...
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bioRxiv - Microbiology 2022Quote: Human antibody concentrations in mouse sera after passive immunization was measured by ELISA using 96-well half-area plates (Corning, #3690) coated with 2 µg/ml sheep anti-Human-IgG (ICN ...
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bioRxiv - Cancer Biology 2022Quote: ... NCI-H1048 (H1048) tumor cells at a density of 2.5×106 per mouse in 50% Matrigel (Cat. No. 356237, Corning, Corning, NY) and PBS mixture were injected subcutaneously (s.c. ...
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bioRxiv - Microbiology 2023Quote: ... cell culture vessels were coated with either 1 µg/ml (EVT) or 2.5 µg/ml (STB) of mouse Collagen IV (Corning™ 354233). For EVT differentiation ...
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bioRxiv - Cancer Biology 2024Quote: Luciferase-expressing mouse ovarian cancer ID8-Luc2 cells were maintained in high glucose Dulbecco’s Modification of Eagle’s Medium (DMEM; Corning, 10-017-CV), 4% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: A BioTek Synergy H1 microplate reader was used to analyze expression kinetics or protein stability of dox-preinduced cells seeded confluent (100 % monolayer) in Costar 96 flat bottom plate (Corning™ 3596) ± dox with Leibovitz’s L-15 phenol red-free (CO2 independent media ...
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bioRxiv - Bioengineering 2020Quote: ... A hydrogel consisting of extracellular matrix (ECM) proteins made of a final concentration of 7.0 mg/ml Type I rat tail collagen (Corning, 354249, MA, USA) was used in all experiments ...
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bioRxiv - Cell Biology 2022Quote: ... 5 μL of the prepared protein standards and lysed MV samples were loaded on a Corning® 96-well plate (Corning, UK). 25 μL working reagent A and 200 μL reagent B were also added ...
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bioRxiv - Biochemistry 2020Quote: ... and 10 μL of protein solution was aliquoted into wells of a 384-well low-volume optical plate (Corning Inc., Corning, New York), and 10 μL of mineral oil was then overlaid onto the protein solution ...
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bioRxiv - Developmental Biology 2020Quote: Wells of 24-well plates were coated with undiluted Matrigel® hESC-Qualified Matrix (protein concentration approximates 10mg/ml)(cat. 354277, Corning®). Matrigel was solidified by incubation for at least 10 minutes at 37°C ...
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bioRxiv - Immunology 2020Quote: ... ZIKV envelope or HIV-1 proteins were coated overnight at 4°C on the wells of 384-well plates (Corning Life Sciences). Each protein was coated at 2 μg/mL in 15 μl in sodium bicarbonate ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were started by adding protein to DNA in a Corning® 384-well Low Volume Black Round Bottom Polystyrene NBS Microplate (Corning #4514). Data are collected for 50 cycles each lasting 20 seconds ...
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bioRxiv - Bioengineering 2022Quote: ... 1×107 pfu of purified baculovirus or 1 μg of NA9 protein were two-fold serial diluted by 1x NA-Flour Assay buffer in black flat-bottom 96-well plates (Corning Inc., NY). Fifty μl of NA-Fluor substrate (Final concentration of 200 μM ...
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bioRxiv - Synthetic Biology 2023Quote: ... The fluorescence intensity (excitation = 485, emission = 510) of 150 μL prey proteins in 96 well plates (Corning® 96 Well CellBIND® Microplates, Corning) using a microplate reader (Agilent BioTek Synergy H4 Hybrid ...
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bioRxiv - Bioengineering 2023Quote: Bone-marrow derived Sprague-Dawley rat mesenchymal stem cells that express red fluorescent protein (RFP-MSCs, P2, Creative Bioarray, Shirley, NY) were cultured in DMEM with 10% FBS and 1% penicillin-streptomycin (Corning, Corning, NY). The wraps were subjected to ethylene oxide gas sterilization and then seeded with 1 x 105 RFP-MSCs ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells expressing fluorescent proteins (mEGFP and mCherry) were plated in 384-well flat clear bottom white polystyrene TC-treated microplates (Corning, Corning, USA) and allowed to attach to plates overnight ...
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bioRxiv - Neuroscience 2023Quote: ... diluted in 0.05 M borate buffer (for protein extraction) or in 24-well plates containing 0 thickness glass coverslips (Assistant Cat#01105209 coated with Matrigel (Corning Cat#356235). On DIV1 80% of medium was exchanged to neuron growth medium (5% fetal bovine serum (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... 10×106 cells/mouse were suspended in a 1:1 solution of serum-free medium and growth factor reduced Matrigel (Corning, NY, USA). 50 µl of cell suspension were injected orthotopically into the anterior prostate lobe of 10-weeks old CD1-nude male mice (Charles River Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor models of parental B16-F10-luciferase or PD-L1-knockout B16-F10-luciferase cells were established by injecting 7.5 × 104 cells per mouse mixed with an equal volume of Matrigel (Corning Inc., Corning, NY) subcutaneously into the flanks of four-to-six-week-old C57BL6 mice ...
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bioRxiv - Neuroscience 2020Quote: ... and plated on a labtek (Starstedt, Nümbrecht, Germany) (1 mouse brain is needed to seed 1 labtek) previously coated with 2 % matrigel (Corning, Steuben, NY, USA) diluted in DMEM 1 g/L glucose ...
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bioRxiv - Biochemistry 2021Quote: NIH-3T3 mouse embryo fibroblast (NIH-3T3 ATCC® CRL-1658) cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Corning 10-013-CV) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... 100 µL of blood was added from each mouse to each well within a 96-well flat bottom plate (Corning Costar, Corning, New York). A dihydrorhodamine (DHR ...
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bioRxiv - Bioengineering 2022Quote: To validate the apoptotic nature of miR-29b delivery to cancer cells human osteosarcoma cells (SaOS2) and mouse osteosarcoma cells (K7M2) were seeded onto 24 well plate with polymer cover slip (Corning, P24-1.5P) at a seeding density of 2×104 cells/cm3 ...
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bioRxiv - Cancer Biology 2023Quote: ... RRM2- and RRM2B- manipulated HepG2 cells were surgically injected into the liver of two-month-old NSG (NOD scid gamma) mice (JAX) at 5 × 104/mouse in 2 µl cold growth factor-reduced (GFR) matrigel (Corning, Corning, New York) using a 5-µl Hamilton syringe and a 27-gauge needle (Hamilton Company ...
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bioRxiv - Developmental Biology 2023Quote: ... The already established H156 and RUNX1C-EGFP H930 hESCs lines were grown on irradiated mouse embryonic fibroblast (MEF) feeders in hES medium defined as DMEM/F12 medium (Corning, L022046-10092CVR) supplemented with 25% of KnockOut™ Serum Replacement (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: Human iPSCs were grown on irradiated mouse embryonic fibroblast (MEF) feeders in hES medium defined as DMEM/F12 medium (Corning, L022046-10092CVR) supplemented with 25% of KnockOut™ Serum Replacement (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... the gRNA complex was mixed with Cas9 protein (IDT, 1081059; 10 μg/μl) in 1x phosphate-buffered saline (1xPBS; Corning, 21-040-CV) to final concentrations of 1.5 μM gRNA complex and 0.25 μg/μl Cas9 protein ...
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bioRxiv - Biochemistry 2020Quote: ... and 10 μL of protein solution was aliquoted into wells of a 384-well low-volume optical plate (Corning Inc., Corning, New York), and 10 μL of mineral oil was then overlaid onto the protein solution ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 µL of the cell-free synthesized fluorescent protein and 45 µL of Milli-Q water were mixed in a 96-well half-area black plate (Corning Incorporated, Corning, NY). The plate was mixed in the plated reader orbitally at medium speed for 15 s and read at the height of 1.5 mm with a gain of 48 ...
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bioRxiv - Synthetic Biology 2020Quote: HEK 293 cells (ATCC) stably over-expressing full-length human ACE2 protein were seeded in 96 well white polystyrene microplates (Corning, Cat. No. CLS3610) at 0.03×106 cells/well in DMEM (10% FBS and 1% Pen-Strep) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 μL of the cell-free synthesized fluorescent protein and 45 μL of Milli-Q water were mixed in a 96-well half-area black plate (Corning Incorporated, Corning, NY). The plate was mixed in the plated reader orbitally at medium speed for 15 s and read at the height of 1.5 mm with a gain of 50 ...
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bioRxiv - Bioengineering 2022Quote: Live mouse chondrogenic cells (ATDC5) (passage 19, 1.0 × 106 cells) were expanded in 100 mm × 20 mm dishes (Corning Inc., New York, USA) using 10 mL DMEM containing 10% FBS and 1% PS and cultured at 37°C under 5% CO2 for 1 week ...
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bioRxiv - Bioengineering 2022Quote: Human embryonic kidney HEK293T cells and RAW264.7 mouse macrophage cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Corning 10-013-CV; Corning, NY, USA) supplemented with 10% EV-depleted fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... RRM2- and RRM2B- manipulated HepG2 cells were surgically injected into the liver of two-month-old NSG (NOD scid gamma) mice (JAX) at 5 × 104/mouse in 2 µl cold growth factor-reduced (GFR) matrigel (Corning, Corning, New York) using a 5-µl Hamilton syringe and a 27-gauge needle (Hamilton Company ...