Labshake search
Citations for Corning :
401 - 450 of 469 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... All buffers used during protein purification were filtered with a 0.22 μm bottle-top vacuum filter (Corning) or with a 0.22 μm Millex syringe filter (Millipore) ...
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bioRxiv - Biochemistry 2020Quote: ... + SAM was set up as 2 µl crystallization drops in (1µl protein:1µl reservoir solution) in 96-well plates (Corning) and equilibrated using commercially available Classics II ...
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bioRxiv - Biophysics 2021Quote: ... The bead/protein mixture was then moved to a 0.22µm cellulose acetate centrifuge filter tube (Spin-X, Corning) and spun for 30s at 13,000xG ...
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bioRxiv - Microbiology 2021Quote: Purified OXA-14 or OXA-935 proteins were set up as 2 µl crystallization drops (1µl protein:1µl reservoir solution) in 96-well plates (Corning) using commercially available Classics II ...
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bioRxiv - Immunology 2022Quote: ... The eluted protein was further purified by SEC with Phosphate-Buffered Saline (PBS, 1×, Corning, 21-040-CM) as the mobile phase on the Superose 6 Increase 10/300 GL (Millipore Sigma ...
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bioRxiv - Microbiology 2020Quote: All NA activity measurements were performed in a 96-well low protein binding black clear bottom plate (Corning). Each sample was mixed with 37°C reaction buffer (0.1 M KH2PO4 pH 6.0 and 1 mM CaCl2 ...
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bioRxiv - Microbiology 2021Quote: ... Purified ZIKV E protein was diluted and added to 96-well microtiter plates (Corning-Costar, Corning, NY, USA) at 0.5 μg/well ...
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bioRxiv - Microbiology 2021Quote: ... Purified ZIKV E protein was diluted and added to 96-well microtiter plates (Corning-Costar, Corning, NY, USA) at 0.5 μg/well ...
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bioRxiv - Immunology 2022Quote: ... 250 ng/well of StrepTag-free tri-S and RBD proteins were coated on ELISA plates (Costar, Corning), which were then blocked ...
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bioRxiv - Bioengineering 2020Quote: Green fluorescent protein positive (GFP+) mosaic analysis with double markers (MADM) OPCs were expanded on T75 (Corning CellBind) treated tissue culture plates ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated using a 5 kDa cut-off concentrator (Corning® Spin-X® UF, England) and flash-frozen in liquid nitrogen for long term storage ...
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bioRxiv - Immunology 2020Quote: ... His-S1 or ecto-spike proteins were plated on half-area high-binding 96-well polystyrene plates (Corning) at 1 μg/mL or 0.5 μg/mL ...
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bioRxiv - Pathology 2023Quote: ... Additional protein blocking was performed by incubating slides in 1X Phosphate buffered saline (PBS) (Corning, 21- 040-CM) containing 5% horse serum and 2.5% Bovine Serum Albumin for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... cells (20 µg of protein) were distributed in 96-well microplates (Corning 3600, white plates with white bottom) and were incubated for 10 min with antagonists ...
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bioRxiv - Microbiology 2021Quote: 200 ng S or RBD protein in coating buffer (pH 9.6) was used to coat 96-well plates (cat.# 9018, Corning) at 4 °C overnight ...
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bioRxiv - Immunology 2021Quote: ... Solubilized CHO cell membrane protein (SMP) was coated onto 96-well half-area high-binding ELISA plates (Corning, 3690) at 5ug/mL in PBS overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: Purified MN100 protein constructs at 1 μg/mL were coated in the wells of 96-well microtiter plates (Corning) in carbonate buffer (50 mM Na2CO3-NaHCO3 ...
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bioRxiv - Cancer Biology 2022Quote: ... protein at 50 nM was prepared and 12.5 μL of this protein solution was aliquoted into 384 well NBS-plates (Corning) containing compound and allowed to incubate for 30 min at room temperature ...
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bioRxiv - Plant Biology 2023Quote: Crystallisation screening was performed using Mosquito-LCP robot (SPT Labtech) in three-drop 96-well Protein Crystallography Plate (Corning). Crystals were grown in a RockImager 1000 system (Formulatrix ...
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bioRxiv - Cell Biology 2023Quote: Cellular immunofluorescence for nuclear envelope proteins was performed using 1205Lu cells plated on cleaned coverslips (#1.5, 22 mm x 22 mm, Corning) for 24-72 hours depending on experimental conditions ...
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bioRxiv - Microbiology 2023Quote: ... and Matrigel® (consisting of a mixture of proteins, mainly laminin, collagen IV, entactin, and heparan sulfate proteoglycan) (Corning). Bacteria were grown on COH plates for 48 hours at 37°C under anaerobic conditions ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1% BSA) and a serial dilution of HQ461.The protein-compound mix was transferred to 384-well plates (Corning 3572); 15 μL per well ...
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bioRxiv - Microbiology 2021Quote: ... was set up at 6-8 mg/mL in loading buffer containing 0 or 500 mM NaCl as 2 μL crystallization drops (1 μL protein: 1 μL reservoir solution) in 96-well plates (Corning) using commercial Classics II ...
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bioRxiv - Cancer Biology 2020Quote: ... cells transfected with pWZL-p53-R175H to overexpress mutant p53 variant R175H (FNEmutp53-R175H) and green-fluorescence protein (GFP) were seeded in ultra low-adhesion plates (Corning). 2% Matrigel was added to the suspended culture after 24 hours to support basement membrane adhesion ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was eluted with Lysis buffer supplemented with 2.5 mM desthiobiotin and concentrated (10k MWCO Corning Spin-X concentrators) at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... DNA and DNA binding proteins were incubated for 30 minutes at room temperature and then transferred to a 384 well plate (Corning). Fluorescence anisotropy was measured using an excitation wavelength of 500 nm and an emission wavelength of 577 nm on a Tecan Spark plate reader fitted with a 50% dichroic 510 mirror ...
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bioRxiv - Synthetic Biology 2022Quote: Excitation and emission spectra of the fluorescent proteins were conducted using a BMG Clariostar Plus microplate reader in sealed plates (Corning, 3370 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded on Biocoat invasion chambers with 8 µm pore size PET membrane precoated with extracellular matrix proteins (Catalog # 354480, Corning). Invasion assays were conducted as per the manufacturer’s protocol using the same seeding conditions as in transwell migration ...
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bioRxiv - Neuroscience 2024Quote: ... The viral supernatant was filtered through a 0.45 µm cellulose acetate low protein binding filter (Corning, Cat # 09-761-35) and stored at 4°C for 2 to 24 hours before further processing ...
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bioRxiv - Microbiology 2024Quote: ... were set up for crystallization as 2 µl crystallization drops (1-µl protein:1-µl reservoir solution) in 96-well (Corning) plates using commercially available Classics II ...
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bioRxiv - Microbiology 2024Quote: The optical density (OD600) measurements for small protein overexpression and deletion constructs were performed in a clear flat bottom 96-well microplate (Corning) containing 150 µL culture per well using a microplate reader (Agilent BioTek Synergy Neo2S) ...
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bioRxiv - Cancer Biology 2024Quote: ... HUVECs of passages four to five were used and cultured in plates coated with bovine collagen type I protein (354231, Corning). All cell lines were grown in a humidified chamber at 37°C supplied with 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... 5,000 cells after three hours phagocytosis assay in the presence of 200 nM hybrid protein were dispensed in a 96-well plate (Corning #3596) at a total volume of 100 µL in serum-free and phenol red-free DMEM ...
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bioRxiv - Biophysics 2022Quote: ... Samples were prepared at room temperature using 0.3 μM labeled protein within the bulk of unlabeled materials and loaded into 96-well plates (Corning nonbinding surface). Sample buffer consisted of 50 mM Tris-HCl buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Mixtures (200 μL) containing YjoB and target proteins in buffer A were placed onto a 96-well microtiter plate (Corning Incorporated). Absorbance at 340 nm was recorded for 150 min with 3 min intervals using a SpectraMax iD5 microplate reader (Molecular Devices ...
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bioRxiv - Physiology 2022Quote: ... samples containing 30 μg (liver) or 15 μg (gonad) of protein were placed in a solid black 96-well plate (Corning 3912), then the reaction was initiated out of direct light by the addition of buffer containing lucigenin and NADPH ...
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bioRxiv - Microbiology 2020Quote: ... Purified NS1 protein from D2Y98P or 16681 (10 ng/well) diluted in PBS was coated onto 96-well EIA plates (Corning costar) overnight at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... 5 μg/ml RBD-SpyTag protein produced in our lab was coated onto 96-well high binding Costar® Assay plates (CORNING) at 4 °C overnight ...
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bioRxiv - Immunology 2020Quote: ELISAs to evaluate antibodies binding to SARS-CoV-2 RBD and trimeric spike proteins were performed by coating of high binding 96 half well plates (Corning #3690) with 50 μL per well of a 1μg/mL protein solution in PBS overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: Peroxidase activity was measured by diluting 20 uL of sample protein extract with 20 uL of 10 mM PBS (pH 6.0) in a 96-well plate (Costar, Corning, Kennebunk, ME). Then ...
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bioRxiv - Immunology 2023Quote: ... was measured by diluting 20 uL of coral protein extract in 20 uL of 50 mM PBS (pH 7.0) in a 96 well plate (Costar, Corning, Kennebunk, ME). In order to convert prophenoloxidase to phenoloxidase ...
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bioRxiv - Bioengineering 2024Quote: ... multilayer Matrigel constructs were made using the same methods described above except replacing the custom hydrogel formulations with Growth Factor Reduced Basement Membrane Matrigel in both layers (9.2 mg/mL protein concentration, Corning, MA, USA).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells expressing fluorescent proteins (mEGFP and mCherry) were plated in 384-well flat clear bottom white polystyrene TC-treated microplates (Corning, Corning, USA) and allowed to attach to plates overnight ...
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bioRxiv - Microbiology 2024Quote: ... Purified NS1 protein from D2Y98P (10 ng/well) diluted in PBS was coated onto 96-well enzyme immunoassay plates (Corning Costar) overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted protein sample was concentrated to a volume of 250 µl using CORNING SPIN-X concentrators (50 kDa cutoff) (Corning, 431490), followed by centrifugation 21,130 g for 15 min ...
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bioRxiv - Bioengineering 2024Quote: ... except the custom hydrogel formulations were replaced with growth factor deficient (GFD) Matrigel basement membrane matrix (9.2 mg/mL protein concentration, Corning, MA, USA) in both layers ...
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bioRxiv - Synthetic Biology 2024Quote: ... NanoLuc activity assays were initiated by adding serially diluted fluorofurimazine to the protein samples prepared in flat white bottom non-binding surface 96-well plates (Corning 3990). The final concentrations of fluorofurimazine ranged from 0.0977 μM to 12.5 μM ...
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bioRxiv - Synthetic Biology 2024Quote: ... FP assays were initiated by adding either SNAP-Cell 647-SiR or SNAP-Surface 549 to the protein samples prepared in flat black bottom non-binding surface 96-well plates (Corning 3991). FP measurements were conducted in 20 second intervals for 80 min at room temperature with a final reaction volume of 100 μL ...
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bioRxiv - Molecular Biology 2021Quote: A BioTek Synergy H1 microplate reader was used to analyze expression kinetics or protein stability of dox-preinduced cells seeded confluent (100 % monolayer) in Costar 96 flat bottom plate (Corning™ 3596) ± dox with Leibovitz’s L-15 phenol red-free (CO2 independent media ...
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bioRxiv - Bioengineering 2020Quote: ... A hydrogel consisting of extracellular matrix (ECM) proteins made of a final concentration of 7.0 mg/ml Type I rat tail collagen (Corning, 354249, MA, USA) was used in all experiments ...